公开(公告)号：KR101150826B1

公开(公告)日：2012-06-11

申请号：KR1020100046741

申请日：2010-05-19

Applicant: 한림대학교 산학협력단 ,  인하대학교 산학협력단 ,  대한민국(농촌진흥청장)

Inventor： 최수영 ,  음원식 ,  박진서 ,  장상호 ,  김대원 ,  조용준 ,  조성민 ,  신승훈 ,  권해용 ,  이광길 ,  강석우 ,  진형준 ,  조세연

IPC: A61F2/02 ,  A61L27/22 ,  C08J3/205 ,  C08L89/00

Abstract: 목적: 본 발명은 뇌척수액이 누수되지 않고, 종래 인공경막과는 달리 생체적합성이 우수하여 세포독성이 없고 수술시 염증을 유발하지 않으면서 투명하여 수술시 안전성을 담보할 수 있는 실크피브로인을 주요 구성 요소로 하는 인공경막을 제공하는 것이다.
방법: 본 발명자들은 누에(
Bombyx mori )로부터 얻은 실크 피브로인을 이용하여 투명한 인공 뇌경막을 제조하였고, 인비트로 및 인비보에서 세포독성 및 염증저해효과를 시험하였다. 실크 피브로인 인공 뇌경막을 가지고 뇌경막재생수술을 동물 모델에 실시하였다. 염증은 조직학적 시험 및 웨스턴 블랏팅으로 측정하였다. 본 발명의 실크 피브로인을 이용한 새로운 인공 뇌경막은 세포 내에서 세포 독성을 나타내지 않았고, 래트에서 일반적인 종래의 뇌경막 재료인 음성대조군과 비교하여 차이가 없었다. 또한, 실크 피브로인 뇌경막은 래트에서 COX-2와 iNOS 발현을 효과적으로 감소시켰다.

公开(公告)号：KR1020120044167A

公开(公告)日：2012-05-07

申请号：KR1020100105590

申请日：2010-10-27

Applicant: 대한민국(농촌진흥청장)

Inventor： 김성렬 ,  강석우 ,  구태원 ,  박승원 ,  이광길 ,  황재삼 ,  윤은영

IPC: C12N15/70 ,  C12N15/12 ,  C12N1/21 ,  C12P21/02

Abstract: PURPOSE: A method for preparing a large amount of papiliocin using recombinant E.coli is provided to ensure high antibacterial activity against various bacteria. CONSTITUTION: A recombinant expression vector pET-KSI/papiliocin contains papiliocin gene. The gene is derived from Papilio xuthus larva. The E.coli BL21(DE3) is prepared by transforming by papiliocin gene-containing recombinant expression vector pET-KSI/Papiliocin. A method for preparing a large amount of recombinant papiliocin comprises: a step of preparing transformed recombinant E.coli; a step of culturing the transformed E.coli to express insoluble recombinant fusion protein; and a step of collecting papiliocin. A pharmaceutical composition for antibiotics contains the recombinant papiliocin as an active ingredient.

Abstract translation: 目的：提供使用重组大肠杆菌制备大量生物素的方法，以确保对各种细菌的高抗菌活性。 构成：重组表达载体pET-KSI / papiliocin含有乳糜酶基因。 该基因衍生自Papilio xuthus幼虫。 通过含有重组体表达载体pET-KSI / Papiliocin的重组表达载体转化制备大肠杆菌BL21（DE3）。 制备大量重组体生物素的方法包括：制备转化重组大肠杆菌的步骤; 培养转化的大肠杆菌以表达不溶性重组融合蛋白的步骤; 和收集乳木素的步骤。 用于抗生素的药物组合物含有重组体乳酸菌素作为活性成分。

公开(公告)号：KR1020100121169A

公开(公告)日：2010-11-17

申请号：KR1020090040200

申请日：2009-05-08

Applicant: 한림대학교 산학협력단 ,  대한민국(농촌진흥청장)

Inventor： 박찬흠 ,  김진 ,  김춘회 ,  권해용 ,  이광길 ,  강석우 ,  여주홍 ,  조유영 ,  우순옥 ,  한상미

IPC: A61F2/18 ,  A61L27/22

CPC classification number: A61L27/3641 ,  A61F2/18 ,  A61F11/00 ,  A61F2002/183 ,  A61L27/3604 ,  A61L27/38 ,  A61L2430/14

Abstract: PURPOSE: An artificial eardrum using a silk protein and a method for manufacturing the same are provided to have strengths in bio compatibility and transparency. CONSTITUTION: An artificial eardrum using a silk protein comprises a silk film shape by desalinating and drying silk protein or silk protein compound solution obtained after removing ceracin from cocoons or fibroin Silk protein or silk protein compound solution are dissolved using chaotropic salt or one compound of bromination lithium, lithium chloride, zinc chloride, or calcium chloride. At least one additive of gelatin, collagen, chitosan, alginic acid, hyaluronic acid, pluronic 127 or PEG is added to the silk film.

Abstract translation: 目的：提供使用丝蛋白的人造耳膜及其制造方法，以具有生物相容性和透明度的优点。 构成：使用丝蛋白的人造鼓膜包括通过脱盐和干燥从茧或丝心蛋白中除去ceracin后获得的丝蛋白或丝蛋白复合溶液的丝膜形状丝蛋白或丝蛋白复合溶液使用离液盐或一种溴化化合物溶解 锂，氯化锂，氯化锌或氯化钙。 将至少一种明胶，胶原，壳聚糖，海藻酸，透明质酸，pluronic 127或PEG的添加剂加入到丝膜中。

公开(公告)号：KR1020100100246A

公开(公告)日：2010-09-15

申请号：KR1020090019022

申请日：2009-03-05

Applicant: 대한민국(농촌진흥청장)

Inventor： 김성렬 ,  황재삼 ,  박관호 ,  이광길 ,  강석우

IPC: C07K14/00

Abstract: PURPOSE: A novel antibacterial peptide gene and antibacterial peptide isolated from Papilio xuthus larva are provided to ensure antibacterial activity against fungi causing candidiasis. CONSTITUTION: An antibacterial peptide gene isolated from Papilio xuthus larva has a base sequence of sequence number 1. An antibacterial peptide encoded by the gene of sequence number 1 has an amino acid sequence of sequence number 2. A pharmaceutical composition contains the antibacterial peptide of sequence number 2 or 3 as an active ingredient.

Abstract translation: 目的：提供从Papilio xuthus幼虫分离的新型抗菌肽基因和抗菌肽，以确保对引起念珠菌病的真菌的抗菌活性。 构成：从Papilio xuthus幼虫分离的抗菌肽基因具有序列号1的碱基序列。由序列号1的基因编码的抗菌肽具有序列号2的氨基酸序列。药物组合物含有序列的抗菌肽 2或3为有效成分。

公开(公告)号：KR1020090072684A

公开(公告)日：2009-07-02

申请号：KR1020070140876

申请日：2007-12-28

Applicant: 대한민국(농촌진흥청장)

Inventor： 구태원 ,  윤은영 ,  최광호 ,  김용보 ,  강석우 ,  강민욱 ,  권오유

IPC: C12N15/31 ,  C07K14/315

Abstract: A promoter and entire genome sequence of heat shock protein isolated from a silk worm is provided to express a excess amount foreign gene and easily select transformant of silk worm. A gene sequence of heat shock protein 70 gene denoted by the sequence 1 comprises gene area and promoter area of heat shock protein 70 isolated silk worm. The Cdna sequence of heat shock protein 70 is denoted by the sequence 2. The amino acid sequence denoted by the sequenc 3 is deduced by cDNA sequence. The maximum promoter activation area of heat shock protein 70 gene comprises 367-1517 sequences.

Abstract translation: 提供从丝虫分离的热休克蛋白的启动子和全基因组序列，以表达过量的外源基因，并容易选择蚕丝的转化体。 由序列1表示的热休克蛋白70基因的基因序列包含热休克蛋白70分离的丝虫的基因面积和启动子面积。 热休克蛋白70的Cdna序列由序列2表示。由序列3表示的氨基酸序列由cDNA序列推导出。 热休克蛋白70基因的最大启动子活化区域包含367-1517个序列。

公开(公告)号：KR1020090032487A

公开(公告)日：2009-04-01

申请号：KR1020070097789

申请日：2007-09-28

Applicant: 대한민국(농촌진흥청장)

Inventor： 김기영 ,  강필돈 ,  김익수 ,  정이연 ,  손봉희 ,  김미자 ,  강석우

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C12Q1/686

Abstract: Provided is a method for discriminating domestic silkworm powder from imported silkworm powder through direct base sequencing analysis. Nine introns are used as silkworm discrimination markers. The nine introns indicate A4Intron1, A4Intron2, PTTHIntron3, LCP30Intron3, LCP30Intron4, SPIIntron3, VDPIntron4, FLCIntron3 and XDHIntron3. A method for discriminating domestic silkworm powder using the direct base sequencing analysis comprises the following steps of: extracting a genome DNA from the silkworm powder; offering information about DNA amplification existence or nonexistence in a specific intron region through PCR(polymerase chain reaction); offering information about discriminable intron regions based on species and primer in the regions through the direct base sequencing analysis; and comparing the species in the intron regions using the silkworm discrimination marker.

Abstract translation: 提供了一种通过直接碱基测序分析鉴别家蚕粉与进口蚕粉的方法。 九个内含子用作蚕鉴别标记。 九个内含子表示A4Intron1，A4Intron2，PTTHIntron3，LCP30Intron3，LCP30Intron4，SPIIntron3，VDPIntron4，FLCIntron3和XDHIntron3。 使用直接碱基测序分析鉴别家蚕粉的方法包括以下步骤：从蚕粉中提取基因组DNA; 通过PCR（聚合酶链反应）提供有关DNA扩增存在或不存在于特定内含子区域的信息; 通过直接碱基测序分析，提供区域内基于物种和引物的可区分内含子区域的信息; 并使用家蚕鉴别标记物比较内含子区域中的物种。

公开(公告)号：KR100790807B1

公开(公告)日：2008-01-04

申请号：KR1020060124569

申请日：2006-12-08

Applicant: 대한민국(농촌진흥청장)

Inventor： 황재삼 ,  윤은영 ,  안미영 ,  황석조 ,  방혜선 ,  강희윤 ,  김연주 ,  김익수 ,  구태원 ,  강석우

IPC: C07K14/00

Abstract: A novel sequence and an antibacterial peptide isolated from larvae of Copris tripartitus are provided to be usefully used as a prophylactic and therapeutic agent of bacterial diseases of human and plant and show antibacterial activity against various pathogenic bacteria. A sequence of an antibacterial peptide isolated from larvae of Copris tripartitus(SEQ ID : NO. 3) is represented by the following : AACGGTTCTG AATTCTTAAC CAGTTCATAC CCAGCTATAA TTTGAATTAC 50 GTCTTTTAAA AAGATGGCAA AATTAATTGC TTTTGCATTA GTAGCTTCGT 100 TATGCCTGTC TATGGTGTTG TGCAATCCGT TACCAGAAGA AGTCCAAGAA 150 GAGGGACTTG TCAGACAAAA AAGGGTAACC TGTGACGTGT TAAGTTTCGA 200 AGCCAAAGGA ATAGCTGTAA ATCATTCGGC TTGTGCGCTA CATTGTATTG 250 CCTTACGTAA AAAAGGTGGT TCTTGTCAAA ATGGTGTGTG TGTTTGTAGA 300 AATTAATTAA CAAGCTGATT GTCCTTTTTT TTAATTGTGG CCGGATGATA 350 TTATTTTCAG TGTAATAAGA ACAAAAATAA AGATTTATAA CTATTAAAAA 400 AAAAAAAAAA AA 412. A novel antibacterial peptide(SEQ ID : NO. 4) is encoded by the sequence and consists of an amino acid sequence of Met Ala Lys Leu Ile Ala Phe Ala Leu Val Ala Ser Leu Cys Leu 15 Ser Met Val Leu Cys Asn Pro Leu Pro Glu Glu Val Gln Glu Glu 30 Gly Leu Val Arg Gln Lys Arg Val Thr Cys Asp Val Leu Ser Phe 45 Glu Ala Lys Gly Ile Ala Val Asn His Ser Ala Cys Ala Leu His 60 Cys Ile Ala Leu Arg Lys Lys Gly Gly Ser Cys Gln Asn Gly Val 75 Cys Val Cys Arg Asn 80.

Abstract translation: 提供从Copris triartae的幼虫分离的新的序列和抗菌肽，有效地用作人和植物的细菌性疾病的预防和治疗剂，并且对各种致病菌具有抗菌活性。 （SEQ ID：NO 3）从Copris tripartitus的幼虫中分离的抗菌肽的序列：AACGGTTCTG AATTCTTAAC CAGTTCATAC CCAGCTATAA TTTGAATTAC 50 GTCTTTTAAA AAGATGGCAA AATTAATTGC TTTTGCATTA GTAGCTTCGT 100 TATGCCTGTC TATGGTGTTG TGCAATCCGT TACCAGAAGA AGTCCAAGAA 150 GAGGGACTTG TCAGACAAAA AAGGGTAACC TGTGACGTGT TAAGTTTCGA 200由下式表示 AGCCAAAGGA ATAGCTGTAA ATCATTCGGC TTGTGCGCTA CATTGTATTG 250 CCTTACGTAA AAAAGGTGGT TCTTGTCAAA ATGGTGTGTG TGTTTGTAGA 300 AATTAATTAA CAAGCTGATT GTCCTTTTTT TTAATTGTGG CCGGATGATA 350 TTATTTTCAG TGTAATAAGA ACAAAAATAA AGATTTATAA CTATTAAAAA 400 AAAAAAAAAA AA 412的新型抗菌肽（SEQ ID：NO：4）是由序列编码，由一个氨基的 Met Ala Lys Leu的氨基酸序列Ile Ala Phe Ala Leu Val Ala Ser Leu Cys Leu 15 Ser Met Val Leu Cys Asn Pro Leu Pro Glu Glu Val Gln Glu Glu 30 Gly Leu Val Arg Gln Lys Arg Val Thr Cys Asp Val Leu Ser Phe 45 Glu Ala Lys Gly Ile Ala Val Asn His Ser Ala Cys Ala Leu His 60 Cys Ile Ala Leu Arg Lys Lys Gly Gly Ser Cys Gln Asn Gly Val 75 Cys Val Cys Arg Asn 80。

公开(公告)号：KR100758813B1

公开(公告)日：2007-09-19

申请号：KR1020050133381

申请日：2005-12-29

Applicant: 대한민국(농촌진흥청장)

Inventor： 남성희 ,  강석우 ,  이상한 ,  홍인표 ,  지상덕 ,  성규병 ,  송병권

IPC: A01G1/00 ,  A01G1/04 ,  A23B4/00

CPC classification number: A01G2/00 ,  A01G18/00 ,  A23B4/00

Abstract: A method for preserving pupa infected with Cordyceps spp, entomopathogenic fungi for a long time and a method for regenerating the fungi are provided to dispense with an expensive equipment or high technology for preserving the fungi, and also to produce fruit bodies on consumer demands by taking out the preserved pupa at desired time. The cultivation time of Cordyceps spp, entomopathogenic fungi can be controlled voluntarily, thereby solving the concentration of production time and the surplus or stock of production. A long-term preserving method of pupa infected with Cordyceps spp, entomopathogenic fungi comprises: preparing the pupa infected with Cordyceps spp, entomopathogenic fungi, controlling the water content of the pupa body to 4%, and then preserving the pupa at 4°C or less, or at -70°C after freeze-drying. The fungi regenerating method comprises soaking the preserved pupa in distilled water for 1~3hr, followed by saturating the pupa with water. The infected pupa can be preserved stably in a water content of 4% at a storage temperature of 4°C or less for 135day, and the regeneration ratio is to about 97%. In addition, a preservation period is more increased at lower temperature in freezing, and is up to 1year in preserving at -70°C or less after freeze-drying.

Abstract translation: 提供了一种用于保存长时间感染冬虫夏草，昆虫病原真菌的蛹的方法和用于再生真菌的方法，以省去用于保存真菌的昂贵的设备或高技术，以及通过采取消费者需求来生产果实 在所需的时间出来保存的蛹。 冬虫夏草，昆虫病原菌的培养时间可以自愿控制，从而解决生产时间和生产过剩或库存的集中。 昆虫病原真菌感染虫蛹的长期保存方法包括：制备感染冬虫夏草，昆虫病原真菌的蛹，将蛹体的水含量控制在4％，然后在4℃保存蛹，或 较少，或在-70℃下冷冻干燥。 真菌再生方法包括将保存的蛹在蒸馏水中浸泡1〜3小时，然后用水饱和蛹。 感染蛹可以在4℃或更低的储存温度下稳定保存在4％的水含量中，135天，再生率为约97％。 另外，冷冻时的温度保持时间更长，冷冻干燥后在-70℃以下保存1年左右。

公开(公告)号：KR1020030002924A

公开(公告)日：2003-01-09

申请号：KR1020010039221

申请日：2001-07-02

Applicant: 대한민국(농촌진흥청장)

Inventor： 구태원 ,  윤은영 ,  황재삼 ,  강석우 ,  권오유

IPC: C12N15/10

Abstract: PURPOSE: Provided are a nucleotide sequence of cDNA coding protein disulfide isomerase(PDI) isolated from the cultured cell of a silkworm, and an amino acid sequence thereof, thereby increasing the expression of useful proteins and consequently mass-producing them economically. CONSTITUTION: The nucleotide sequence of cDNA coding protein disulfide isomerase(PDI) isolated from the cultured cell of a silkworm, is represented by the SEQ ID NO:1, and its amino acid sequence is represented by the SEQ ID NO:2.

Abstract translation: 目的：提供从蚕的培养细胞分离的cDNA编码蛋白质二硫键异构酶（PDI）的核苷酸序列及其氨基酸序列，从而增加有用蛋白质的表达，从而经济地批量生产它们。 构成：从蚕的培养细胞分离的cDNA编码蛋白质二硫键异构酶（PDI）的核苷酸序列由SEQ ID NO：1表示，其氨基酸序列由SEQ ID NO：2表示。

公开(公告)号：KR100331169B1

公开(公告)日：2002-04-06

申请号：KR1019990054812

申请日：1999-12-03

Applicant: 대한민국(농촌진흥청장)

Inventor： 김근영 ,  서동상 ,  황재삼 ,  이상몽 ,  강석우

IPC: C12N15/00

Abstract: 본발명은미세유리주사침을이용하여 DNA를누에알에직접주입하는방법에관한것이다. 상기의방법은미세유리관끝을사면으로예리하게갈아날카롭게다듬은미세유리주사침으로, 외래유전자를함유하는 DNA용액을누에알껍질을뚫고, 알내부로한번에직접주사하는것으로서, 외래유전자를생식세포에직접발현시키는뛰어난효과가있다.