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公开(公告)号：AU2012223526B2
公开(公告)日：2017-03-09
申请号：AU2012223526
申请日：2012-02-28
Applicant: HARVARD COLLEGE
Inventor： INGBER DONALD E , KIM HYUN JUNG
Abstract: The embodiments of the invention described herein relate to systems and methods for culturing and/or maintaining intestinal cells, tissues and/or organoids in vitro. The cells, tissues and/or organoids cultured according to the methods and systems described herein can mimic or reproduce natural intestinal epithelial structures and behavior as well as support co-culture of intestinal microflora.
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72.发明专利
公开(公告)号：CA2987271A1
公开(公告)日：2016-12-01
申请号：CA2987271
申请日：2016-05-22
Applicant: HARVARD COLLEGE , THE CHILREN'S MEDICAL CENTER CORP
Inventor： INGBER DONALD E , JAIN ABHISHEK , VAN DER MEER ANDRIES D , MICHELSON ALAN DAVID , FRELINGER ANDREW L III , BARRILE RICCARDO
Abstract: A method is directed to determining a thrombosis function and includes flowing a fluid sample over a surface having a fixed endothelial cell monolayer. The method further includes stimulating the fixed endothelial cell monolayer to induce formation of a clot, the clot being formed via interaction between the fixed endothelial cell monolayer and the fluid sample. In response to the clot formation, the method further includes determining a thrombosis function associated with the fluid sample and the fixed endothelial cell monolayer.
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公开(公告)号：CA2982749A1
公开(公告)日：2016-10-20
申请号：CA2982749
申请日：2016-04-08
Applicant: HARVARD COLLEGE
Inventor： DOMANSKY KAREL , HINOJOSA CHRISTOPHER DAVID , INGBER DONALD E , LEVNER DANIEL , THOMPSON GUY II
Abstract: A microfluidic device for determining a response of cells comprises a microchannel and a seeding channel. The microchannel is at least partially defined by a porous membrane having cells adhered thereto. The microchannel has a first cross-sectional area. The seeding channel delivers a working fluid to the cells within the microchannel. The seeding channel has a second cross-sectional area that is less than the first cross-sectional area such that a flow of the working fluid produces a substantially higher shear force within the seeding channel to inhibit the attachment of cells within the seeding channel. And when multiple seeding channels are used to deliver fluids to multiple microchannels that define an active cellular layer across the membrane, the seeding channels are spatially offset from each other such that fluid communication between the fluids occurs only at the active region via the membrane, not at the seeding channels.
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公开(公告)号：AU2015261681A1
公开(公告)日：2016-01-07
申请号：AU2015261681
申请日：2015-11-27
Applicant: HARVARD COLLEGE
Inventor： INGBER DONALD E , FERNANDEZ JAVIER GOMEZ
Abstract: The present invention is directed to a method of forming a composite laminar material comprising layers of a carbohydrate and protein with the preferred components being chitosan as the carbohydrate and silk fibroin as the protein. The method of producing this material results in a composite material of high mechanical strength. The invention also provides a method of attaching a medical implant device to tissue.
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公开(公告)号：CA2944220A1
公开(公告)日：2015-09-17
申请号：CA2944220
申请日：2014-12-19
Applicant: HARVARD COLLEGE , FERNANDEZ-ALCON JOSE , WEN NORMAN , NOVAK RICHARD
Inventor： FERNANDEZ-ALCON JOSE , INGBER DONALD E , HAMILTON GERALDINE A , HINOJOSA CHRISTOPHER , DOMANSKY KAREL , LEVNER DANIEL , THOMPSON GUY II , HAJIPOURAN BENAM KAMBEZ , VILLENAVE REMI , UMUNDUM THOMAS , PARIS ALFRED , BAUER GEORG , WEN NORMAN , NOVAK RICHARD
Abstract: An organomimetic device includes a microfluidic device that can be used to culture cells in its microfluidic channels. The organomimetic device can be part of dynamic system that can apply mechanical forces to the cells by modulating the microfluidic device and the flow of fluid through the microfluidic channels. The membrane in the organomimetic device can be modulated mechanically via pneumatic means and/or mechanical means. The organomimetic device can be manufactured by the fabrication of individual components separately, for example, as individual layers that can be subsequently laminated together.
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Patent Agency Ranking
公开(公告)号：CA2934662A1
公开(公告)日：2015-09-17
申请号：CA2934662
申请日：2014-12-19
Applicant: HARVARD COLLEGE
Inventor： INGBER DONALD E , HAJIPOURAN BENAM KAMBEZ , VILLENAVE REMI , HAMILTON GERALDINE A , HASSELL BRYAN , HINOJOSA CHRISTOPHER D
IPC: C12M3/02
Abstract: Provided herein relates to systems and methods for producing and using a body having a central channel separated by one or more membranes. The membrane(s) are configured to divide the central channel into at least one mesochannel and at least one microchannel. The height of the mesochannel is substantially greater than the height of the microchannel. A gaseous fluid can be applied through the mesochannel while a liquid fluid flowing through the microchannel. The systems and methods described herein can be used for various applications, including, e.g., growth and differentiation of primary cells such as human lung cells, as well as any other cells requiring low shear and/also stratified structures, or simulation of a microenvironment in living tissues and/or organs (to model physiology or disease states, and/or to identify therapeutic agents and/or vaccines). The systems and methods can also permit co-culture with one or more different cell types.
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77.发明专利
公开(公告)号：CA2917889A1
公开(公告)日：2015-01-15
申请号：CA2917889
申请日：2014-07-11
Applicant: HARVARD COLLEGE
Inventor： INGBER DONALD E , LEVNER DANIEL , THOMPSON GUY III , FERNANDEZ-ALCON JOSE , HINOJOSA CHRISTOPHER DAVID
Abstract: Systems and methods interconnect cell culture devices and/or fluidic devices by transferring discrete volumes of fluid between devices. A liquid-handling system collects a volume of fluid from at least one source device and deposits the fluid into at least one destination device. In some embodiments, a liquid-handling robot actuates the movement and operation of a fluid collection device in an automated manner to transfer the fluid between the at least one source device and the at least one destination device. In some cases, the at least one source device and the at least one destination device are cell culture devices. The at least one source device and the at least one destination device may be microfluidic or non-microfluidic devices. In some cases, the cell culture devices may be microfluidic cell culture devices. In further cases, the microfluidic cell culture devices may include organ-chips.
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公开(公告)号：SG194437A1
公开(公告)日：2013-12-30
申请号：SG2013072228
申请日：2012-04-02
Applicant: HARVARD COLLEGE , CHILDRENS MEDICAL CENTER
Inventor： COOPER RYAN M , INGBER DONALD E , SUPER MICHAEL , KANG JOO HUN , SCHULTE ALEXA , TERRY RICHARD , KALISH DAVID , DOMANSKY KAREL , YUNG CHONG WING
Abstract: A dialysis like therapeutic (DLT) device is provided. The DLT device includes at least one source channel connected at least one collection channels by one or more transfer channels. Fluid contacting surface of the channels can be an anti-fouling surface such as slippery liquid-infused porous surface (SLIPS). Fluids can be flown at high flow rates through the channels. The target components of the source fluid can be magnetic or bound to magnetic particles using an affinity molecule. A source fluid containing magnetically bound target components can be pumped through the source channel of the microfluidic device. A magnetic field gradient can be applied to the source fluid in the source channel causing the magnetically bound target components to migrate through the transfer channel into the collection channel. The collection channel can include a collection fluid to flush the target components out of the collection channel. The target components can be subsequently analyzed for detection and diagnosis. The source channel and the collection channels of the microfluidic device are analogous to the splenic arterioles and venules, respectively; the transfer channels mimic the vascular sinusoids of the spleen where opsonized particles are retained. Thus, the device acts as a dialysis like therapeutic device by combining fluidics and magnetics.
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公开(公告)号：AU2012236128A1
公开(公告)日：2013-10-31
申请号：AU2012236128
申请日：2012-04-02
Applicant: CHILDRENS MEDICAL CENTER , HARVARD COLLEGE
Inventor： COOPER RYAN M , INGBER DONALD E , SUPER MICHAEL , KANG JOO HUN , SCHULTE ALEXA , KALISH DAVID , TERRY RICHARD , DOMANSKY KAREL , YUNG CHONG WING
IPC: G01N33/569 , B81B1/00 , G01N35/08 , G01N35/10
Abstract: A dialysis like therapeutic (DLT) device is provided. The DLT device includes at least one source channel connected at least one collection channels by one or more transfer channels. Fluid contacting surface of the channels can be an anti-fouling surface such as slippery liquid-infused porous surface (SLIPS). Fluids can be flown at high flow rates through the channels. The target components of the source fluid can be magnetic or bound to magnetic particles using an affinity molecule. A source fluid containing magnetically bound target components can be pumped through the source channel of the microfluidic device. A magnetic field gradient can be applied to the source fluid in the source channel causing the magnetically bound target components to migrate through the transfer channel into the collection channel. The collection channel can include a collection fluid to flush the target components out of the collection channel. The target components can be subsequently analyzed for detection and diagnosis. The source channel and the collection channels of the microfluidic device are analogous to the splenic arterioles and venules, respectively; the transfer channels mimic the vascular sinusoids of the spleen where opsonized particles are retained. Thus, the device acts as a dialysis like therapeutic device by combining fluidics and magnetics.
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公开(公告)号：AU2012223526A1
公开(公告)日：2013-09-12
申请号：AU2012223526
申请日：2012-02-28
Applicant: HARVARD COLLEGE
Inventor： INGBER DONALD E , KIM HYUN JUNG
Abstract: The embodiments of the invention described herein relate to systems and methods for culturing and/or maintaining intestinal cells, tissues and/or organoids in vitro. The cells, tissues and/or organoids cultured according to the methods and systems described herein can mimic or reproduce natural intestinal epithelial structures and behavior as well as support co-culture of intestinal microflora.
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