公开(公告)号：AT484001T

公开(公告)日：2010-10-15

申请号：AT04725288

申请日：2004-04-02

Applicant: LEICA MICROSYSTEMS

Inventor： BIRK HOLGER ,  STORZ RAFAEL ,  SEYFRIED VOLKER

IPC: G02B21/06 ,  G01T1/29 ,  G02B21/26

公开(公告)号：DE50115464D1

公开(公告)日：2010-06-10

申请号：DE50115464

申请日：2001-06-01

Applicant: LEICA MICROSYSTEMS

Inventor： BIRK HOLGER ,  STORZ RAFAEL

IPC: G02B6/00 ,  G02B21/00 ,  G02B5/00 ,  G02B5/04 ,  G02B5/18 ,  G02B5/22 ,  G02B6/02 ,  G02B6/12 ,  G02B6/122 ,  G02B6/255 ,  G02B21/06 ,  G02B27/00 ,  G02F1/39 ,  H01S3/00 ,  H01S3/16

Abstract: The arrangement has a scanning microscope e.g. confocal microscope, and a laser formed as a pulsed laser (1). An optical component is positioned between the laser and a lens (12), where light generated by the laser is spectrally processed by a unique cycle such that a spectral illumination light is emitted from the laser. An optical fiber (20) forms the optical component, and is comprised of photonic-bad-gap material. An optical diode is provided between the laser and fiber for suppressing back reflection of light rays originated from ends of the fiber.

公开(公告)号：DE502004003515D1

公开(公告)日：2007-05-31

申请号：DE502004003515

申请日：2004-07-21

Applicant: LEICA MICROSYSTEMS

Inventor： STORZ RAFAEL ,  KNEBEL WERNER

IPC: G02B21/00 ,  G02B21/06

Abstract: The scanning microscope (1) has an incoupling apparatus (31) with which light (33) other than the light (17) proceeding from the sample (7) is coupled into the detection beam path and conveyed to the detector (21). A closure apparatus automatically closes off the incoupling apparatus from the outside, in largely light-tight fashion, when the light guide (37) guiding the other light to the incoupling apparatus is removed. An independent claim is also included for confocal scanning microscope.

公开(公告)号：DE10251345B4

公开(公告)日：2006-08-17

申请号：DE10251345

申请日：2002-11-05

Applicant: LEICA MICROSYSTEMS

Inventor： HOPPE MARTIN ,  KNEBEL WERNER ,  MOELLMANN KYRA ,  STORZ RAFAEL

IPC: A61B5/00 ,  A61B5/02 ,  G02B21/24

Abstract: The invention concerns a method and an apparatus for investigating layers ( 1 ) of tissues in living animals using a microscope ( 2 ). The microscope ( 2 ) is focused onto a layer ( 1 ), and images of the layer ( 1 ) are acquired or optical measurements are performed on it. Positional changes of the layer ( 1 ) are brought about by movements of the animal or of its organs. The positional changes are sensed, and corresponding signals are generated. The signals are stored, together with the corresponding images or measurement results, for later evaluation; or they are processed in such a way that the positional changes are compensated for in order to investigate the layer ( 1 ). As a result, the layer ( 1 ) can be qualitatively or quantitatively investigated microscopically, irrespective of the movement of the animal or its organs.

公开(公告)号：DE10357584B4

公开(公告)日：2006-06-14

申请号：DE10357584

申请日：2003-12-08

Applicant: LEICA MICROSYSTEMS

Inventor： SEYFRIED VOLKER ,  STORZ RAFAEL

IPC: G02B21/00 ,  G02B21/06 ,  G01J3/44 ,  G02B21/16

Abstract: Separating different emission wavelengths in a scanning microscope, is new. Separating different emission wavelengths in a scanning microscope comprises: (a) scanning a specimen with an illuminating light beam by passing the illuminating light beam over the specimen using a beam deflector; (b) selectively applying each of excitation wavelengths (1, 2, 3) to the illuminating light beam during the scanning according to a pre-definable illumination scheme; (c) detecting emission light coming from the specimen using detector(s), where the detector is read out upon each selective applying of a respective excitation wavelength to provide respective corresponding detected signals; and (d) associating the detected signals with the respective excitation wavelength using the illumination scheme. The emission light includes emission wavelengths corresponding to the excitation wavelengths. An independent claim is also included for an apparatus for separating different emission wavelengths in a scanning microscope, comprising: (a) light source(s) configured to generate an illuminating light beam; (b) a beam deflector configured to scan a specimen with the illuminating light beam by passing the illuminating light beam over the specimen; (c) a light control device configured to selectively apply each of excitation wavelengths to the illuminating light beam during a scanning according to a pre-definable illumination scheme; (d) detector(s) configured to pick up emission light coming from the specimen; and (e) a processing device configured to associate the detected signals with the respective excitation wavelengths using the illumination scheme.

公开(公告)号：DE102004058565A1

公开(公告)日：2006-04-20

申请号：DE102004058565

申请日：2004-12-03

Applicant: LEICA MICROSYSTEMS

Inventor： BIRK HOLGER ,  STORZ RAFAEL ,  FEHRER DIRK-OLIVER ,  KRESS CLAUS

IPC: G02B21/00

Abstract: Scanning microscope, especially a confocal scanning microscope with scanning device (1), detector device (2), an electronic device (3) for operating the microscope, and a cooling device (4) for at least one of the components, which uses a liquid coolant, especially water, and includes a galvanometer.

公开(公告)号：DE10340020B4

公开(公告)日：2005-08-11

申请号：DE10340020

申请日：2003-08-28

Applicant: LEICA MICROSYSTEMS

Inventor： BIRK HOLGER ,  SEYFRIED VOLKER ,  STORZ RAFAEL

IPC: G01J3/14 ,  G01J3/28 ,  G01J3/36 ,  G01J3/44 ,  G01N21/64 ,  G02B21/00 ,  G01J3/02

公开(公告)号：DE10313988A1

公开(公告)日：2004-10-14

申请号：DE10313988

申请日：2003-03-27

Applicant: LEICA MICROSYSTEMS

Inventor： SEYFRIED VOLKER ,  STORZ RAFAEL

IPC: G02B21/00 ,  G01M11/02

Abstract: The microscope is tested using a phosphorescent probe (5). The probe is thermally stimulated until phosphorescent saturation. Light emitted from the probe is detected by a detector (7) and the detected signal is evaluated and compared with known data.

公开(公告)号：DE10261155A1

公开(公告)日：2004-07-15

申请号：DE10261155

申请日：2002-12-20

Applicant: LEICA MICROSYSTEMS

Inventor： STORZ RAFAEL

IPC: G02B21/00

Abstract: Method for imaging an object (3, 4) with a confocal scanning microscope, whereby the object is moved in a first direction with a predetermined scanning velocity and in a second direction with a slower scanning velocity in order to obtain information relating to the object. The assignment of object information to individual pixels (25) is dependent on the temporal execution of the raster process. An Independent claim is made for a confocal scanning microscope for imaging an object.

公开(公告)号：DE10159239A1

公开(公告)日：2003-06-26

申请号：DE10159239

申请日：2001-12-03

Applicant: LEICA MICROSYSTEMS

Inventor： ENGELHARDT JOHANN ,  STORZ RAFAEL

IPC: G02B7/04 ,  G02B21/00 ,  G02B21/02 ,  G02B21/24

Abstract: The microscope objective (1) has several lenses (7,9,11,13) together displaceable within an objective housing. A drive unit (17) displaces the lenses in the objective housing. Independent claims are included for the following: (a) the microscope; and (b) the imaging of a specimen using microscope objective.