公开(公告)号：AT107692T

公开(公告)日：1994-07-15

申请号：AT87830033

申请日：1987-01-28

Applicant: SCLAVO SPA

Inventor： RAPPUOLI RINO ,  NICOSIA ALFREDO ,  ARICO MARIA BEATRICE

IPC: C12N15/09 ,  A61K39/00 ,  A61K39/095 ,  A61K39/10 ,  C07K14/00 ,  C07K14/195 ,  C07K14/235 ,  C07K14/41 ,  C12N1/00 ,  C12N15/00 ,  C12N15/83 ,  C12P21/02 ,  C12Q1/68 ,  C12R1/19 ,  G01N33/50 ,  C12N1/20

Abstract: Cloning and sequencing of the EcoRI fragment of B. pertussis chromosomal DNA with 4696 base pairs, conta ining the genes which code for the five subunits of the pertussis toxin. A hybrid plasmid containing the DNA fragment or its further fragments and a micro-organism transformed by the hybrid plasmid and capable of expressing the cloned DNA fragment or further fragments thereof by synthesis of the pertussis toxin or one or more subunits of the pertussis toxin. The pertussis toxine or one or more subunits of the pertussis toxin so obtained are useful for the preparation of vaccines and diagnostic kits.

公开(公告)号：AU643190B2

公开(公告)日：1993-11-11

申请号：AU4515589

申请日：1989-10-20

Applicant: UNIV LELAND STANFORD JUNIOR ,  SCLAVO SPA

Inventor： RELMAN DAVID A ,  DOMENIGHINI MARIO ,  RAPPUOLI RINO ,  FALKOW STANLEY

IPC: C12N15/09 ,  A61K31/70 ,  A61K38/00 ,  A61K39/00 ,  A61K39/10 ,  A61K39/395 ,  A61P37/04 ,  C07K14/235 ,  C12N1/21 ,  C12N5/10 ,  C12N9/12 ,  C12P21/02 ,  C12R1/01 ,  C12R1/19 ,  C07K13/00 ,  C12N15/31

公开(公告)号：AT85811T

公开(公告)日：1993-03-15

申请号：AT88120994

申请日：1988-12-15

Applicant: ENIRICERCHE SPA ,  SCLAVO SPA

Inventor： PEDRONI PAOLA ,  RIBOLI BARBARA ,  DE FERRA FRANCESCA ,  GRANDI GUIDO ,  TOMA SALVATORE ,  ARICO BEATRICE ,  RAPPUOLI RINO

IPC: C12N1/20 ,  A61K38/00 ,  A61K39/00 ,  A61K39/10 ,  A61P31/04 ,  A61P37/04 ,  C07K14/00 ,  C07K14/195 ,  C07K14/235 ,  C07K14/41 ,  C12N1/19 ,  C12N1/21 ,  C12N15/30 ,  C12P21/02 ,  C12R1/01 ,  C12R1/19

Abstract: The cloning and sequencing of the gene which codes for a new pilinic subunit of Bordetella pertussis are described. The aminoacid sequence of the mature subunit, deduced from its necleotide sequence, is similar but not identical to that of the known pilins 2, 3 and 6. Polypeptides having the aminoacid sequence of the mature pilinic subunit or of regions thereof are particularly useful for the development of synthetic acellular vaccines against pertussis.

公开(公告)号：DK76191A

公开(公告)日：1991-06-14

申请号：DK76191

申请日：1991-04-25

Applicant: UNIV LELAND STANFORD JUNIOR ,  SCLAVO SPA

Inventor： RELMAN DAVID A ,  DOMENIGHINI MARIO ,  RAPPUOLI RINO ,  FALKOW STANLEY

IPC: C12N15/09 ,  A61K31/70 ,  A61K38/00 ,  A61K39/00 ,  A61K39/10 ,  A61K39/395 ,  A61P37/04 ,  C07K14/235 ,  C12N1/21 ,  C12N5/10 ,  C12N9/12 ,  C12P21/02 ,  C12R1/01 ,  C12R1/19 ,  C12N15/62 ,  C07K13/00 ,  C12N15/31 ,  C12N15/70

公开(公告)号：CA2017180A1

公开(公告)日：1990-11-19

申请号：CA2017180

申请日：1990-05-18

Applicant: SCLAVO SPA

Inventor： DE MAGISTRIS MARIA T ,  BARTOLONI ANTONELLA ,  RAPPUOLI RINO ,  TAGLIABUE ALDO

IPC: A61K38/00 ,  A61K39/10 ,  A61K39/39 ,  A61P37/00 ,  C07K7/06 ,  C07K7/08 ,  C07K14/235 ,  C07K19/00

Abstract: SYNTHETIC PEPTIDES CAPABLE OF STIMULATING A T-CELLULAR IMMUNITY The S1 subunit regions of pertussis toxin from Bordetella pertussis comprising the antigenic sites recognized by the human T helper clones anti-S1 are described and said sites are characterized. Synthetic peptides having an amino acid sequence corresponding to the sequence of said regions or of the T-epitopes comprised therein are novel. Said peptides, alone or in combination with the immunodominant Bepitope of the pertussis toxin S1 subunit from Bordetella pertussis, are particularly useful as immunogens for manufacturing acellular anti-pertussis vaccines.

公开(公告)号：IT1196453B

公开(公告)日：1988-11-16

申请号：IT2103186

申请日：1986-07-04

Applicant: SCLAVO SPA

Inventor： RAPPUOLI RINO ,  PERUGINI MARIA ,  RATTI GIULIO

IPC: C12N15/09 ,  C07K14/34 ,  C12N1/20 ,  C12N15/00 ,  C12Q1/68 ,  C12Q1/689 ,  C12R1/15 ,  C12R1/19 ,  C12P

公开(公告)号：CH660375A5

公开(公告)日：1987-04-15

申请号：CH28684

申请日：1984-01-23

Applicant: SCLAVO SPA

Inventor： RAPPUOLI RINO

IPC: C12N15/09 ,  A61K39/05 ,  C07K14/34 ,  C12N15/00 ,  C12N15/12 ,  C12P21/00 ,  C12R1/16

公开(公告)号：LU85197A1

公开(公告)日：1984-11-08

申请号：LU85197

申请日：1984-02-07

Applicant: SCLAVO SPA

Inventor： RAPPUOLI RINO

IPC: C12N15/09 ,  A61K39/05 ,  C07K14/34 ,  C12N15/00 ,  C12N15/12 ,  C12P21/00 ,  C12R1/16 ,  C12P ,  C12N ,  C12R

Abstract: Process for the production of a protein correlated with the diphtheric toxin which comprises culturing in a liquid nutrient medium having a concentration of iron ions of from 0.05 mu g/ml to 0.5 mu g/ml, at a temperature of from 30 DEG C. to 40 DEG C., in a neutral culturing environment and under aerobic conditions, a microorganism belonging to the Corynebacterium diphtheriae genus with two mutant phages encoding the protein correlated with the diphtheric toxin integrated in a nontandem mode in their chromosomes.