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公开(公告)号：KR1020130054498A
公开(公告)日：2013-05-27
申请号：KR1020110117788
申请日：2011-11-11
Applicant: 한국생명공학연구원 , 대한민국(농촌진흥청장)
CPC classification number: C12Q1/701 , C12Q1/04 , C12Q1/6837
Abstract: PURPOSE: A probe set for diagnosing or detecting CVX(cactus virus X) is provided to minimize damage due to viral diseases in agricultural products and to enhance product yield. CONSTITUTION: An oligonucleotide probe set for diagnosing or detecting CVX contains one or more oligonucleotides selected from the group consisting of oligonucleotides in sequence numbers 2203-2205 and complementary oligonucleotides thereof. A method for diagnosing or detecting CVX comprises: a step of isolating RNA from a sample; a step of synthesizing cDNA using the RNA; a step of hybridizing the cDNA with the probe set; and a step of detecting hybridization.
Abstract translation: 目的：提供诊断或检测CVX（仙人掌病毒X）的探针，以尽量减少农产品病毒性疾病造成的损害，并提高产品产量。 构成：用于诊断或检测CVX的寡核苷酸探针组含有选自序列号2203-2205的寡核苷酸和其互补寡核苷酸的一种或多种寡核苷酸。 用于诊断或检测CVX的方法包括：从样品中分离RNA的步骤; 使用RNA合成cDNA的步骤; 将cDNA与探针组杂交的步骤; 和检测杂交的步骤。
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公开(公告)号：KR1020130054497A
公开(公告)日：2013-05-27
申请号：KR1020110117784
申请日：2011-11-11
Applicant: 한국생명공학연구원 , 대한민국(농촌진흥청장)
CPC classification number: C12Q1/701 , C12Q1/04 , C12Q1/6837
Abstract: PURPOSE: A probe set for diagnosing or detecting CPMMV(cowpea mild mottle virus) is provided to minimize damage due to viral diseases in crops and to enhance production. CONSTITUTION: An oilgonucleotide probe set for diagnosing or detecting CPMMV contains one or more oligonucleotides selected from the group consisting of oligonucleotides in sequence numbers 578-587 and complementary oligonucleotides thereof. A method for diagnosing or detecting CPMMV comprises: a step of isolating RNA from a sample; a step of synthesizing cDNA using the RNA as a template; a step of hybridizing the cDNA with the probe set; and a step of identifying the hybridization. [Reference numerals] (AA) Green pea; (BB) Host: Green pea
Abstract translation: 目的：提供用于诊断或检测CPMMV（cow豆轻度斑驳病毒）的探针，以尽量减少由于作物病毒性疾病引起的损伤，并提高产量。 构成：用于诊断或检测CPMMV的石油核苷酸探针组含有选自序列号578-587的寡核苷酸和其互补寡核苷酸的一种或多种寡核苷酸。 一种用于诊断或检测CPMMV的方法包括：从样品中分离RNA的步骤; 使用RNA作为模板合成cDNA的步骤; 将cDNA与探针组杂交的步骤; 以及鉴定杂交的步骤。 （附图标记）（AA）绿豆; （BB）主持人：绿豆
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公开(公告)号：KR1020130054496A
公开(公告)日：2013-05-27
申请号：KR1020110117760
申请日：2011-11-11
Applicant: 한국생명공학연구원 , 대한민국(농촌진흥청장)
CPC classification number: C12Q1/701 , C12Q1/04 , C12Q1/6837
Abstract: PURPOSE: A probe set for diagnosing or detecting PSV(peanut stunt virus) is provided to minimize damage due to viral diseases in crops and to enhance production. CONSTITUTION: An oligonucleotide probe set for diagnosing or detecting PSV contains one or more oligonucleotides selected from the group consisting of oligonucleotides in sequence numbers 1025-1034, 1045-1049, and 2988, and complementary oligonucleotides thereof. A method for diagnosing or detecting PSV comprises: a step of isolating RNA from a sample; a step of synthesizing cDNA; a step of hybridizing the cDNA with the probe set; and a step of detecting hybridization of the cDNA and the probe set. [Reference numerals] (AA) Cowpea; (BB) Host: cowpea; (CC) Kidney bean; (DD) Host: Kidney bean
Abstract translation: 目的：提供用于诊断或检测PSV（花生特技病毒）的探针，以尽量减少作物病毒性疾病引起的损伤，并提高产量。 构成：用于诊断或检测PSV的寡核苷酸探针组含有选自序列号1025-1034,1045-1049和2988的寡核苷酸及其互补寡核苷酸的一种或多种寡核苷酸。 用于诊断或检测PSV的方法包括：从样品中分离RNA的步骤; 合成cDNA的步骤; 将cDNA与探针组杂交的步骤; 以及检测cDNA和探针组杂交的步骤。 （AA）Cow豆; （BB）主持人：cow豆; （CC）芸豆; （DD）主机：芸豆
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公开(公告)号：KR1020130054494A
公开(公告)日：2013-05-27
申请号：KR1020110117752
申请日：2011-11-11
Applicant: 한국생명공학연구원 , 대한민국(농촌진흥청장)
CPC classification number: C12Q1/701 , C12Q1/04 , C12Q1/6837
Abstract: PURPOSE: A probe set for diagnosing or detecting BBWV2(Broad bean wilt virus2) is provided to minimize damage due to viral diseases in crops and to increase product yield. CONSTITUTION: An oligonucleotide probe set for diagnosing or detecting BBWV2 contains one or more oligonucleotides selected from the group consisting of oligonucleotides in sequence numbers 1143-1152 and 1158-1164 and complementary oligonucleotides thereof. A method for diagnosing or detecting BBWV2 comprises: a step of isolating RNA from a sample; a step of synthesizing cDNA using RNA as a template; a step of hybridizing the probe set with cDNA; and a step of detecting hybridization.
Abstract translation: 目的：提供用于诊断或检测BBWV2（蚕豆枯萎病毒2）的探针，以尽量减少作物病毒性疾病造成的损伤，并提高产品产量。 构成：用于诊断或检测BBWV2的寡核苷酸探针组含有选自序列号1143-1152和1158-1164的寡核苷酸及其互补寡核苷酸的一种或多种寡核苷酸。 一种用于诊断或检测BBWV2的方法包括：从样品中分离RNA的步骤; 使用RNA作为模板合成cDNA的步骤; 探针组与cDNA杂交的步骤; 和检测杂交的步骤。
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公开(公告)号：KR1020130054484A
公开(公告)日：2013-05-27
申请号：KR1020110117611
申请日：2011-11-11
Applicant: 한국생명공학연구원 , 대한민국(농촌진흥청장)
CPC classification number: C12Q1/701 , C12Q1/04 , C12Q1/6837
Abstract: PURPOSE: A probe set for diagnosing Genus Carmovirus is provided to minimize viral diseases in crops and to enhance product yields. CONSTITUTION: An oligonucleotide probe set for diagnosing or detecting Genus Carmovirus contains one or more oligonucleotides selected from a group consisting of oligonucleotides of sequence numbers 674, 682-685, 691-692, 730-734, 746-748, 1452-1458, 1665-1672, 2371, and 2745-2754, and complementary oligonucleotides thereof. A method for diagnosing or detecting Genus Carmovirus comprises: a step of isolating RNA from a sample which is suspected to have Genus Carmovirus infection; a step of synthesizing cDNA; a step of hybridizing the cDNA and the probe set; and a step of detecting hybridization. [Reference numerals] (AA) Jangyeobkong; (BB) Host: Jangyeobkong
Abstract translation: 目的：提供用于诊断卡门诺病毒属的探针，以尽量减少作物中的病毒性疾病，并提高产品产量。 构成：用于诊断或检测属Carmovirus属的寡核苷酸探针组含有一种或多种选自序列号674,682-685,691-692,730-734,746-748,1452-1458,1665的寡核苷酸的寡核苷酸 -1672,2731和2745-2754及其互补寡核苷酸。 用于诊断或检测属Carmovirus的方法包括：从怀疑具有Carmovirus类病毒感染的样品中分离RNA的步骤; 合成cDNA的步骤; cDNA和探针组杂交的步骤; 和检测杂交的步骤。 （附图标记）（AA）Jangyeobkong; （BB）主持人：Jangyeobkong
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Patent Agency Ranking
公开(公告)号：KR101161284B1
公开(公告)日：2012-08-01
申请号：KR1020090003073
申请日：2009-01-14
Applicant: 대한민국(농촌진흥청장)
IPC: G01N33/569 , C12N15/33 , C12Q1/68
Abstract: 본 발병은 포도 등에 발생하고 있는 포도부채잎바이러스(GFLV)를 진단하기 위한 PCR 검사시스템에 관한 것이다. PCR 검사시스템은 GFLV 진단용 최적 프라이머 조합 및 이에 부합하는 검정용 프라이머 조합(nested primer)과 이를 뒷받침할 수 있는 종 특이 프라이머 조합 및 양성대조구를 포함하고 있다.
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公开(公告)号：KR101099617B1
公开(公告)日：2011-12-28
申请号：KR1020090004637
申请日：2009-01-20
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/70 , G01N33/569 , C12Q1/68
Abstract: 본 발명은 벼 줄무늬잎마름병 바이러스 감염 식물체에 애멸구를 접종시켜 바이러스를 보독시키는 단계; 전기 단계에 의해 얻어진 애멸구를 혼합하여 마쇄하는 단계를 포함하는 벼 줄무늬잎마름병 바이러스 보독충 진단용 양성대조군의 제조방법을 제공한다.
벼 줄무늬잎마름병 바이러스, 애멸구, 진단-
公开(公告)号：KR100994420B1
公开(公告)日：2010-11-15
申请号：KR1020080098927
申请日：2008-10-09
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 서열번호 1 내지 13으로 표시된 프라이머 중에서 선택된 프라이머 세트를 포함하는 GLV, LYSV, GCLV, OYDV 및 알렉시바이러스를 동시 진단하기 위한 프라이머 세트, 상기 프라이머 세트를 포함하는 GLV, LYSV, GCLV, OYDV 및 알렉시바이러스를 동시 진단하기 위한 키트, 및 상기 프라이머 세트를 이용하여 GLV, LYSV, GCLV, OYDV 및 알렉시바이러스를 동시 진단하는 방법에 관한 것이다.
본 발명의 진단법은 지금까지 사용된 진단 방법에서 불가능하던 마늘에 발생하는 모든 바이러스(13종)을 가장 경제적이고 간편한 방법으로 진단하며, 동시에 최고의 검출한계를 가지고 있다. 이 진단법은 마늘 바이러스 무병주 생산에 사용될 수 있다.
마늘, 바이러스, 진단법, 다중진단, multiplex RT-PCR, GLV, LYSV, GCLV, OYDV, Allexivirus-
公开(公告)号：KR1020100082962A
公开(公告)日：2010-07-21
申请号：KR1020090002284
申请日：2009-01-12
Applicant: 대한민국(농촌진흥청장) , 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: C12N15/10 , C12N15/11 , C12Q1/6851 , C12Q1/6853 , C12Q1/701
Abstract: PURPOSE: A primer combination for raspberry ringspot virus(RpRSV) and a method for diagnosing RpRSV are provided to diagnose with high detection limit. CONSTITUTION: A primer combination for diagnosing RpRSV comprises forward primer and reverse primer. The forward primer is selected from base sequences of sequence numbers 1, 2, 4, 5, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, and 19. The reverse primer is selected from base sequences of sequence numbers 23, 24, 25, 26, 28, 31, 32, 33, 34, 35, 36, 39, and 41. The primer combination for diagnosing RpRSV comprises a primer of sequence numbers 12 and 28, sequence number 11 and 28, or sequence numbers 8 and 31. A method for specifically diagnosing RpRSV comprises: a step of isolating total RNA from a plant sample; a step of amplifying a target seqeuence by RT-PCR; and a step of detecting the PCR product.
Abstract translation: 目的：提供覆盆子环斑病毒（RpRSV）的引物组合和诊断RpRSV的方法，以高检出限进行诊断。 构成：用于诊断RpRSV的引物组合包括正向引物和反向引物。 正向引物选自序列号1,2,4,5,7,8,9,10,11,12,13,14,15,16,17和19的碱基序列。反向引物选自 序列号23,24,25,26,28,31,32,33,34,35,36,39和41的碱基序列。用于诊断RpRSV的引物组合包含序列号12和28的引物，序列号 11和28，或序列号8和31.用于特异性诊断RpRSV的方法包括：从植物样品分离总RNA的步骤; 通过RT-PCR扩增靶序列的步骤; 以及检测PCR产物的步骤。
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公开(公告)号：KR1020100039933A
公开(公告)日：2010-04-19
申请号：KR1020080098924
申请日：2008-10-09
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/701 , C12Q1/6827
Abstract: PURPOSE: A specific primer for diagnosing garlic common latent virus(GCLV) is provided to simply and economically diagnose GCLV with improved detection ability. CONSTITUTION: A primer set for diagnosing GCLV comprises forward primer of sequence number 1 or 2 and reverse primer of sequence number 2 or 3. A primer set for diagnosing comprises a primer set of sequence numbers 1 and 2, primer set of sequence numbers 1 and 3, or primer set of sequence numbers 4 and 3. A kit for diagnosing GCLV comprises the primer set and reagent for RT-PCR.
Abstract translation: 目的：提供一种诊断大蒜常见潜伏病毒（GCLV）的特异性引物，简化和经济地诊断GCLV，提高检测能力。 构成：用于诊断GCLV的引物组包括序列号1或2的正向引物和序列号2或3的反向引物。用于诊断的引物组包括序列号1和2的引物组，序列号1的引物组和 3或序列号4和3的引物组。用于诊断GCLV的试剂盒包含用于RT-PCR的引物组和试剂。
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