Abstract:
The present invention relates to a method for screening genes used for increasing 1,4-butanediol (BDO) production by using proteomics data. In the present invention, 1,4-BDO productivity is increased when a Ncgl0630 (citrate synthase) protein and a Ncgl2145 (hypothetical protein) protein are overly expressed wherein the Ncgl0630 (citrate synthase) protein and the Ncgl2145 (hypothetical protein) protein are screened via the method which is used to find proteins related to 1,4-BDO productivity and accordingly increases productivity, thereby being widely applicable industrially.
Abstract:
The present invention relates to a strain that produces 4-hydroxybutyrate (4HB) wherein the activity of malate quinone oxidoreductase or phosphoenolpyruvate carboxykinase is reduced or removed; or introduces a polynucleotide that codes alpha-ketoglutarate synthase or mutants thereof, or pyruvate carboxykinase or mutants thereof, thereby improving the productivity of 4HB. Also, the strain has an additonally improved function of producing 4HB by introducing polynucleotide that codes pyruvate dehydrogenase and formate dehydrogenase. In the present invention, the transgenic strain in an anaerobic fermentation is used for producing 4HB in a high yield rate, thus being very useful in industrial application.
Abstract:
Disclosed in the invention are a composite anode material, a negative electrode and a lithium battery comprising the same, and a preparation method of the composite anode material, wherein the composite anode material includes a shell containing a hollow carbon fiber; and a core which is located inside a hollow hole of the carbon fiber and include a first metal nanostructure and a conductive material.
Abstract:
One embodiment of the present invention relates to a metabolism network model of Corynebacterium glutamicum for producing 1,4-BDO by Corynebacterium glutamicum, which is a strain that does not produce 1,4-BDO in a natural state. On the basis of the network model, metabolic properties of Corynebacterium glutamicum are analyzed. By predicting effects of a deletion target enzyme (ldhA, mqo or mdh) in a state in which Cat1, sucD, 4hbD, cat2 and adhE are included, and removing them, production efficiency of 1,4-BDO and increase of 1,4-BDO under the same fermentation conditions are confirmed. In addition, transformation microorganism produced by the method can produce 1,4-BDO in high efficiency, and therefore, can be effectively used in the industries.
Abstract:
PURPOSE: A composite negative active material is provided to improve lifetime and structure stability, thereby improving lifetime of a lithium secondary battery. CONSTITUTION: A composite negative active material comprises a porous carbon material; and a metal nanostructure arranged in one or more pores inside or on the surface of the porous carbon material. The metal nanocomposite is grown on the basis of a metal catalyst particle arranged inside or on the surface of the porous carbon material. A lithium secondary battery comprises a positive electrode which includes a positive electrode active material, a negative electrode which includes a negative electrode active material; and electrolyte which is inserted between the positive electrode and negative electrode. The negative electrode active material comprises the composite negative electrode active material.