公开(公告)号：KR1020120129851A

公开(公告)日：2012-11-28

申请号：KR1020120116121

申请日：2012-10-18

Applicant: 한국해양과학기술원

Inventor： 이정현 ,  이현숙 ,  강성균 ,  김윤재 ,  배승섭 ,  전정호 ,  임재규 ,  김상진 ,  권개경 ,  차선신

IPC: C12N9/16 ,  C12N15/55 ,  C12N15/63

Abstract: PURPOSE: An esterase KTL7 which is separated from the bottom of the sea is provided to enhance activities and to have stability in wide range of temperature. CONSTITUTION: An esterase KTL7 which is separated from the bottom of the sea has amino acid sequence of the sequence number 32(SEQ ID NO:32). A gene ciphering the esterase has the base sequence described in the sequence number 31. A manufacturing method of the esterase gene comprises the following steps: transforming the cells into recombinant vector which includes the esterase of the sequence number 31; culturing the transformed cells; and separating the esterase from the cultured cell. The novel esterase gene is obtained by the following steps: extracting environmental DNA from the deposit of the depth of 1,400 M; building a library by using fosmid vector; and separating the gene from a clone which is active in the tricaprulin flat medium.

Abstract translation: 目的：提供与海底分离的酯酶KTL7，以增强活性，并在宽温度范围内具有稳定性。 构成：从海底分离出的酯酶KTL7具有序列号32（SEQ ID NO：32）的氨基酸序列。 加密酯酶的基因具有序列号31中描述的碱基序列。酯酶基因的制备方法包括以下步骤：将细胞转化成包含序列号31的酯酶的重组载体; 培养转化细胞; 并从培养的细胞中分离酯酶。 通过以下步骤获得新型酯酶基因：从1400m深度的沉积物中提取环境DNA; 使用fosmid载体构建库; 并将该基因与三权纲平台培养基中活性的克隆分离。

公开(公告)号：KR1020120097647A

公开(公告)日：2012-09-05

申请号：KR1020110016983

申请日：2011-02-25

Applicant: 한국해양과학기술원

Inventor： 강성균 ,  진욱 ,  이현숙 ,  이정현 ,  권개경 ,  김상진 ,  김윤재 ,  황영옥 ,  임형순 ,  조용균

IPC: G01N33/574 ,  C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/158 ,  G01N33/574 ,  G01N33/57415 ,  G01N33/57438 ,  G01N33/5748 ,  C12Q1/6837 ,  G01N33/5302 ,  G01N2033/57403 ,  G01N2033/57461

Abstract: PURPOSE: A method for diagnosing cancer using MEST is provided to improve the accuracy and reliability of diagnosis for breast cancer and liver cancer. CONSTITUTION: A kit for diagnosing cancer contains an antibody or aptamer which specifically binds to MEST(mesoderm specific transcript homolog) protein, a nucleotide sequence encoding the MEST protein, a complementary sequence to the nucleotide, or a fragment of the nucleotide. The cancer is metastatic cancer. The kit is an immunoassay kit. A method for detecting a marker for diagnosing cancer or analyzing cancer prognosis comprises a step of detecting the expression of MEST protein or a nucleotide sequence encoding the protein from a biological sample.

Abstract translation: 目的：提供使用MEST诊断癌症的方法，以提高乳腺癌和肝癌诊断的准确性和可靠性。 构成：用于诊断癌症的试剂盒含有特异性结合MEST（中胚层特异性转录同系物）蛋白的抗体或适体，编码MEST蛋白的核苷酸序列，与核苷酸的互补序列或核苷酸的片段。 癌症是转移性癌症。 该试剂盒是免疫测定试剂盒。 用于检测用于诊断癌症或分析癌症预后的标志物的方法包括从生物样品检测MEST蛋白的表达或编码该蛋白质的核苷酸序列的步骤。

公开(公告)号：KR1020110126009A

公开(公告)日：2011-11-22

申请号：KR1020100045689

申请日：2010-05-14

Applicant: 한국해양과학기술원

Inventor： 이정현 ,  강성균 ,  김상진 ,  권개경 ,  이현숙 ,  김윤재 ,  배승섭 ,  임재규 ,  전정호 ,  조요나 ,  황영옥 ,  차선신

IPC: C12N9/12 ,  C12N15/54 ,  C12N15/63

Abstract: PURPOSE: A DNA polymerase P7 and genes thereof are provided to ensure excellent processivity, fidelity, and elongation length performance. CONSTITUTION: A DNA polymerase P7 contains an amino acid sequence of sequence number 2. A DNA polymerase P7 gene encodes an amino acid sequence of sequence number 2. The DNA polymerase P7 gene contains a base sequence of sequence number 1. A recombinant vector contains the P7 gene. A host cell is transformed with the recombinant vector containing the P7 gene. The DNA polymerase P7 is derived from Thermococcus sp.

Abstract translation: 目的：提供DNA聚合酶P7及其基因以确保优异的持续性，保真度和伸长长度性能。 构成：DNA聚合酶P7含有序列号2的氨基酸序列.DNA聚合酶P7基因编码序列号2的氨基酸序列.DNA聚合酶P7基因含有序列号1的碱基序列。重组载体含有 P7基因。 用含有P7基因的重组载体转化宿主细胞。 DNA聚合酶P7衍生自Thermococcus sp。

公开(公告)号：KR1020110093913A

公开(公告)日：2011-08-18

申请号：KR1020117014743

申请日：2009-09-07

Applicant: 한국해양과학기술원

Inventor： 이정현 ,  강성균 ,  이현숙 ,  김상진 ,  권개경 ,  차선신 ,  전정호 ,  임형순 ,  김윤재 ,  배승섭 ,  임재규 ,  김민식 ,  김태완 ,  류지영 ,  정재연

IPC: C12N9/02 ,  C12N15/53 ,  C12P3/00 ,  C12N1/21

CPC classification number: C12N9/0006 ,  C12N9/0067 ,  C12P3/00 ,  C12R1/01 ,  Y02P20/132 ,  Y02P20/52

Abstract: PURPOSE: A hydrogenase isolated from Thermococcus spp. is provided to generate hydrogen at room temperature and pressure without harmful by-products. CONSTITUTION: A hydrogenase contains an amino acid sequence of sequence number 2. A gene encoding the hydrogenase contains a base sequence of sequence number 13. A method for producing hydrogen from Thermococcus spp. comprises: a step of culturing Thermococcus spp. in a medium containing formate at 80°C under the anaerobic condition; and a step of isolating hydrogen from a culture container.

Abstract translation: 目的：从Thermococcus spp。分离的氢化酶。 被提供以在室温和压力下产生氢气而没有有害的副产物。 构成：氢化酶含有序列号2的氨基酸序列。编码氢化酶的基因含有序列号13的碱基序列。从Thermococcus spp。生产氢的方法。 包括：培养Thermococcus spp的步骤。 在含有甲酸盐的培养基中，在80℃，无氧条件下， 以及从培养容器中分离氢的步骤。

公开(公告)号：KR1020080108921A

公开(公告)日：2008-12-16

申请号：KR1020080054757

申请日：2008-06-11

Applicant: 한국해양과학기술원

Inventor： 김상진 ,  강성균 ,  권개경 ,  이정현 ,  이현숙 ,  강지현 ,  손재학

IPC: C12N9/50 ,  C12N9/00

CPC classification number: C12N9/50 ,  A01N63/00

Abstract: The proteinase with the algicidal activity is provided to inhibit the growth of red-tide plankton, thereby processing the red-tide generation region effectively. The proteinase with the algicidal activity has an amino acid sequence selected from SEQ ID NOs:1, 4-7, 26 and 27, and is produced from Kordia algicida OT1(KCTC 11320BP). A gene encoding the proteinase with the algicidal activity has a nucleotide sequence selected from SEQ ID NOs:2, 8-11, 28 and 29. An algicidal formulation comprises the proteinase with the algicidal activity.

Abstract translation: 提供具有杀藻活性的蛋白酶以抑制红潮浮游生物的生长，从而有效地处理红潮生成区域。 具有杀藻活性的蛋白酶具有选自SEQ ID NO：1,47,26和27的氨基酸序列，并且由Kordia algicida OT1（KCTC 11320BP）生产。 编码具有杀藻活性的蛋白酶的基因具有选自SEQ ID NO：2,8-11,28和29的核苷酸序列。杀藻剂制剂包含具有杀藻活性的蛋白酶。