公开(公告)号：CA2842321A1

公开(公告)日：2013-01-24

申请号：CA2842321

申请日：2012-07-18

Applicant: HARVARD COLLEGE

Inventor： INGBER DONALD E ,  SUPER MICHAEL ,  WAY JEFFREY CHARLES ,  CARTWRIGHT MARK J ,  BERTHET JULIA B ,  SUPER DINAH R ,  ROTTMAN MARTIN M ,  WATTERS ALEXANDER

IPC: G01N33/543 ,  A61K47/48 ,  C07K14/47 ,  C07K14/705 ,  G01N33/569

Abstract: Described herein are engineered microbe-targeting or microbe-binding molecules, kits comprising the same and uses thereof. Some particular embodiments of the microbe-targeting or microbe-binding molecules comprise a carbohydrate recognition domain of mannose-binding lectin, or a fragment thereof, linked to a portion of a Fc region. In some embodiments, the microbe-targeting molecules or microbe-binding molecules can be conjugated to a substrate, e.g., a magnetic microbead, forming a microbe-targeting substrate (e.g., a microbe-targeting magnetic microbead). Such microbe-targeting molecules and/or substrates and the kits comprising the same can bind and/or capture of a microbe and/or microbial matter thereof, and can thus be used in various applications, e.g., diagnosis and/or treatment of an infection caused by microbes such as sepsis in a subject or any environmental surface. Microbe-targeting molecules and/or substrates can be regenerated after use by washing with a low pH buffer or buffer in which calcium is insoluble.

公开(公告)号：AU2002306862A1

公开(公告)日：2002-11-05

申请号：AU2002306862

申请日：2002-03-25

Applicant: HARVARD COLLEGE ,  CHILDRENS MEDICAL CENTER

Inventor： INGBER DONALD E ,  OSTUNI EMANUELE ,  CHEN CHRISTOPHER S ,  WHITESIDES GEORGE M

IPC: B01L3/00 ,  C12N11/08 ,  C12N11/02 ,  C07K17/02 ,  C12N5/00 ,  C07K17/08

Abstract: The present invention is directed to a method of patterning materials, such as proteins, on a contoured surface by depositing them onto protrusions on the surface and to a cell containment device that may be constructed by this method. In one embodiment, the present invention is directed to a method of selectively depositing a material on a substrate including a contoured surface including a protrusion and a recess. The method includes applying a first fluid to the contoured surface of the substrate and allowing the first fluid to distribute across a portion of the contoured surface such that the first fluid contacts the protrusion and not the recess. The method also includes allowing a first material to deposit on the substrate where the substrate is in contact with the first fluid. Optionally, this method may further include applying a second fluid to the contoured surface of the substrate, allowing the second fluid to distribute across a portion of the contoured surface such that the second fluid contacts the recess, and allowing a second material to deposit on the substrate where the substrate is in contact with the second fluid. Optionally, the method may still further include applying a third fluid to the contoured surface of the substrate, allowing the third fluid to distribute across a portion of the contoured surface, and allowing a third material with an affinity for one of the first material and the second material to deposit on the substrate only where the one of the first material and the second material is deposited. In one embodiment of this method the first material is a cytophobic material, the second material is a cytophilic material and the third material is a cell. In another embodiment, the present invention is directed to a cell containment device, including a substrate having a contoured surface, a recess in the surface of the substrate, a cytophobic material connected to the surface of the substrate outside the recess and a cytophilic material connected to the recess.

公开(公告)号：AU2018307822B2

公开(公告)日：2024-06-27

申请号：AU2018307822

申请日：2018-07-27

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： HENRY OLIVIER YVES FREDERIC ,  INGBER DONALD E ,  SABATE DEL RIO JONATHAN ,  JOLLY PAWAN

IPC: C09D5/24 ,  C08K3/20

Abstract: Carbon nanotubes or graphene combined with proteinaceous material forming compositions that can be coated on surfaces are described. For example, the described compositions can be used as a coating on an electrode. The coatings can be functionalized with capture agents, targeting specific analytes. In addition to being conductive, the coatings prevent fouling and passivation of the electrodes by non-specific binding. This allows the coated electrodes to be used in complex matrices such as can be found in biological fluids and tissues. The coated electrodes can be regenerated and reused repeatedly.

公开(公告)号：AU2017326179B2

公开(公告)日：2022-12-08

申请号：AU2017326179

申请日：2017-09-13

Applicant: HARVARD COLLEGE

Inventor： INGBER DONALD E ,  KASENDRA MAGDALENA ,  SONTHEIMER-PHELPS ALEXANDRA ,  TOVAGLIERI ALESSIO

IPC: C12N5/071 ,  A61L27/36 ,  C12N5/078 ,  C12N5/28 ,  C12Q1/18

Abstract: Described herein are methods for providing an in vitro intestinal model system, e.g., using primary cells instead of cell lines and/or cancerous cells.

公开(公告)号：CA2842321C

公开(公告)日：2022-05-03

申请号：CA2842321

申请日：2012-07-18

Applicant: HARVARD COLLEGE

Inventor： INGBER DONALD E ,  SUPER MICHAEL ,  WAY JEFFREY CHARLES ,  CARTWRIGHT MARK J ,  BERTHET JULIA B ,  SUPER DINAH R ,  ROTTMAN MARTIN M ,  WATTERS ALEXANDER

IPC: C07K19/00 ,  A61K38/16 ,  A61K47/66 ,  A61P31/04 ,  C07K14/47 ,  C07K16/00 ,  C07K17/00 ,  C12Q1/68 ,  G01N33/569

Abstract: Described herein are engineered microbe-targeting or microbe-binding molecules, kits comprising the same and uses thereof. Some particular embodiments of the microbe-targeting or microbe-binding molecules comprise a carbohydrate recognition domain of mannose-binding lectin, or a fragment thereof, linked to a portion of a Fc region. In some embodiments, the microbe-targeting molecules or microbe-binding molecules can be conjugated to a substrate, e.g., a magnetic microbead, forming a microbe-targeting substrate (e.g., a microbe-targeting magnetic microbead). Such microbe-targeting molecules and/or substrates and the kits comprising the same can bind and/or capture of a microbe and/or microbial matter thereof, and can thus be used in various applications, e.g., diagnosis and/or treatment of an infection caused by microbes such as sepsis in a subject or any environmental surface. Microbe-targeting molecules and/or substrates can be regenerated after use by washing with a low pH buffer or buffer in which calcium is insoluble.

公开(公告)号：AU2016364932B2

公开(公告)日：2021-11-04

申请号：AU2016364932

申请日：2016-12-02

Applicant: HARVARD COLLEGE

Inventor： VARONE ANTONIO ,  WEN NORMAN ,  LEVNER DANIEL ,  NOVAK RICHARD ,  MCPARTLIN LORI ,  INGBER DONALD E ,  CHOE YOUNGJAE ,  LENG LIAN ,  NGUYEN JUSTIN K

IPC: C12M1/00 ,  C12M1/34 ,  C12M1/42 ,  C12M3/00 ,  C12M3/04 ,  C12Q1/02

Abstract: A device for simulating a function of a tissue includes a first structure, a second structure, and a membrane. The first structure defines a first chamber. The first chamber includes a matrix disposed therein and an opened region. The second structure defines a second chamber. The membrane is located at an interface region between the first chamber and the second chamber. The membrane includes a first side facing toward the first chamber and a second side facing toward the second chamber. The membrane separates the first chamber from the second chamber.

公开(公告)号：AU2019253903A1

公开(公告)日：2019-11-14

申请号：AU2019253903

申请日：2019-10-25

Applicant: HARVARD COLLEGE

Inventor： HAMILTON GERALDINE A ,  HINOJOSA CHRISTOPHER ,  DOMANSKY KAREL ,  LEVNER DANIEL ,  THOMPSON II GUY ,  HAJIPOURAN BENAM KAMBEZ ,  VILLENAVE REMI ,  UMUNDUM THOMAS ,  PARIS ALFRED ,  BAUER GEORG ,  FERNANDEZ-ALCON JOSE ,  WEN NORMAN ,  NOVAK RICHARD ,  INGBER DONALD E

IPC: B01L3/00 ,  C12M3/00

Abstract: An organomimetic device comprising: a. a first microchannel height-defining layer having a bottom surface and a first microchannel disposed in the bottom surface wherein the first microchannel height-defining layer comprises: i. a first lamination layer having a first microchannel aperture therein, wherein thickness of the first lamination layer defines the height of the first microchannel; and ii. a first sealing layer disposed on top of the first lamination layer, wherein the first sealing layer provides a top closure of the first microchannel aperture, thereby forming the first microchannel; b. a second microchannel height-defining layer having a top surface and a second microchannel disposed in the top surface; and c. a membrane layer having a membrane portion, the membrane layer being laminated between the bottom surface of the first microchannel height-defining layer and the top surface of the second microchannel height-defining layer, wherein a first surface portion of the membrane portion provides a lower boundary of the first microchannel and a second surface portion of the membrane portion provides an upper boundary of the second microchannel; and wherein at least a portion of the first microchannel is aligned with at least a portion of the second microchannel on an opposite side of the membrane portion. 242 200 244 207 206 202 209 230 -- ,232

公开(公告)号：AU2014386207B2

公开(公告)日：2019-08-22

申请号：AU2014386207

申请日：2014-12-19

Applicant: HARVARD COLLEGE

Inventor： VILLENAVE REMI ,  UMUNDUM THOMAS ,  PARIS ALFRED ,  BAUER GEORG ,  FERNANDEZ-ALCON JOSE ,  WEN NORMAN ,  NOVAK RICHARD ,  INGBER DONALD E ,  HAMILTON GERALDINE A ,  HINOJOSA CHRISTOPHER ,  DOMANSKY KAREL ,  LEVNER DANIEL ,  THOMPSON II GUY ,  HAJIPOURAN BENAM KAMBEZ

IPC: B01L3/00 ,  C12M3/00

Abstract: An organomimetic device includes a microfluidic device that can be used to culture cells in its microfluidic channels. The organomimetic device can be part of dynamic system that can apply mechanical forces to the cells by modulating the microfluidic device and the flow of fluid through the microfluidic channels. The membrane in the organomimetic device can be modulated mechanically via pneumatic means and/or mechanical means. The organomimetic device can be manufactured by the fabrication of individual components separately, for example, as individual layers that can be subsequently laminated together.

公开(公告)号：AU2017254907B2

公开(公告)日：2019-08-15

申请号：AU2017254907

申请日：2017-11-01

Applicant: HARVARD COLLEGE

Inventor： INGBER DONALD E ,  SUPER MICHAEL ,  WAY JEFFREY CHARLES ,  CARTWRIGHT MARK J ,  WATTERS ALEXANDER ,  BERTHET JULIA B ,  SUPER DINAH R ,  ROTTMAN MARTIN M

IPC: C07K14/47 ,  C07K14/705 ,  G01N33/543 ,  G01N33/569

Abstract: [005841 Described herein are engineered microbe-targeting or microbe-binding molecules, kits comprising the same and uses thereof. Some particular embodiments of the microbe targeting or microbe-binding molecules comprise a carbohydrate recognition domain of mannose-binding lectin, or a fragment thereof, linked to a portion of a Fc region. In some embodiments, the microbe-targeting molecules or microbe-binding molecules can be conjugated to a substrate, e.g., a magnetic microbead, forming a microbe-targeting substrate (e.g., a microbe-targeting magnetic microbead). Such microbe-targeting molecules and/or substrates and the kits comprising the same can bind and/or capture of a microbe and/or microbial matter thereof, and can thus be used in various applications, e.g., diagnosis and/or treatment of an infection caused by microbes such as sepsis in a subject or any environmental surface. Microbe-targeting molecules and/or substrates can be regenerated after use by washing with a low pH buffer or buffer in which calcium is insoluble.

公开(公告)号：CA3075534A1

公开(公告)日：2019-05-16

申请号：CA3075534

申请日：2018-09-18

Applicant: HARVARD COLLEGE

Inventor： GOYAL GIRIJA ,  HASSELL BRYAN ,  INGBER DONALD E

IPC: C12N5/07 ,  A61K35/36 ,  C12M1/34 ,  C12N5/0789

Abstract: Disclosed herein are devices and methods for generating orthotopic models of cancer. The devices and methods include providing a microfluidic device having a body, the body including a first microchannel separated from a second microchannel by an at least partially porous membrane, the membrane having a first side facing the first microchannel and a second side facing the second microchannel, seeding the first side of the membrane with healthy cells and cancer cells such that the cancer cells are seeded with a differentiated tissue layer, and culturing the healthy cells and the cancer cells within the microfluidic device by flowing medium through one or more of the first and second microchannels with or without endothelium in the second channel.