公开(公告)号：KR1020030062126A

公开(公告)日：2003-07-23

申请号：KR1020020002561

申请日：2002-01-16

Applicant: (주)케비젠

Inventor： 엄수종 ,  장판식 ,  김소미 ,  박찬수 ,  한혜숙 ,  신원필 ,  신홍식 ,  박시호 ,  김동명

IPC: C12N15/53

CPC classification number: C12N9/0069 ,  C12P7/02 ,  C12Y113/11063

Abstract: PURPOSE: Mutant genes of human beta, beta-carotene 15, 15'-dioxygenase having improved enzyme activity are provided, so that enzyme proteins expressed from the mutant genes can be used in various fields related to the vitamin A production. CONSTITUTION: Mutant genes of human beta, beta-carotene 15, 15'-dioxygenase are produced by error-prone PCR using a human beta, beta-carotene 15, 15'-dioxygenase gene as templates, and have the enzyme activity of the expressed protein of 1100 pmol/h. A method for producing vitamin A comprises digesting beta-carotene using the expressed human beta, beta-carotene 15, 15'-dioxygenase. A method for producing vitamin A comprises transforming a host cell using the mutant genes of human beta, beta-carotene 15, 15'-dioxygenase, and culturing the transformed host cell in a medium, wherein the host cell is a plant cell, a prokaryotic cell, a yeast cell, an insect cell, a mammal cell or a poultry cell.

Abstract translation: 目的：提供具有改善的酶活性的人β，β-胡萝卜素15,15'-双加氧酶的突变基因，使得从突变基因表达的酶蛋白可用于与维生素A生产相关的各种领域。 构成：使用人β，β-胡萝卜素15,15'-双加氧酶基因作为模板，通过易错PCR产生人β，β-胡萝卜素15,15'-双加氧酶的突变基因，并具有表达的酶活性 蛋白质为1100 pmol / h。 生产维生素A的方法包括使用表达的人β，β-胡萝卜素15,15'-双加氧酶消化β-胡萝卜素。 生产维生素A的方法包括使用人β，β-胡萝卜素15,15'-双加氧酶的突变基因转化宿主细胞，并在培养基中培养转化的宿主细胞，其中宿主细胞是植物细胞，原核 细胞，酵母细胞，昆虫细胞，哺乳动物细胞或家禽细胞。