公开(公告)号：KR1020020085491A

公开(公告)日：2002-11-16

申请号：KR1020010025012

申请日：2001-05-08

Applicant: (주)케비젠 ,  신홍식

Inventor： 신홍식 ,  박명순 ,  노영쇠 ,  권윤자 ,  박시호 ,  엄수종 ,  장판식 ,  조성동

IPC: C07C227/18

Abstract: PURPOSE: A novel method for preparing α-L-aspartyl-L-phenylalanine methyl ester·HCl salt is provided, to reduce the amount of by-products, to allow the by-products to be reused after hydrolysis and to increase the production yield by 20% or more. CONSTITUTION: The method comprises the steps of reacting L-aspartic acid with trimethyl silyl chloride in the presence of an alcohol solvent to esterify the β-COOH selectively to obtain α-L-aspartyl-L-phenylalanine methyl ester·HCl. Preferably the alcohol solvent is selected from the group consisting of methyl alcohol, ethyl alcohol, allyl alcohol and benzyl alcohol. Preferably the method comprises further the steps of protecting an amine group with -CHO, Boc, benzyloxycarbonyl group, or -C(O)OR¬2 (R2 is methyl. ethyl or isobutyl group) by using acetic anhydride and formic acid.

Abstract translation: 目的：提供一种制备α-L-天冬氨酰-L-苯丙氨酸甲酯·HCl盐的新方法，以减少副产物的含量，使副产物在水解后再次使用，并提高产量 减少20％以上。 方法：该方法包括在醇溶剂存在下使L-天冬氨酸与三甲基甲硅烷基氯反应，选择性地酯化β-COOH，得到α-L-天冬氨酰-L-苯丙氨酸甲酯·HCl。 醇溶剂优选选自甲醇，乙醇，烯丙醇和苄醇。 优选地，所述方法还包括通过使用乙酸酐和甲酸保护具有-CHO，Boc，苄氧基羰基或-C（O）OR 2（R2为甲基，乙基或异丁基）的胺基的步骤。

公开(公告)号：KR100424251B1

公开(公告)日：2004-03-22

申请号：KR1020010025012

申请日：2001-05-08

Applicant: (주)케비젠 ,  신홍식

Inventor： 신홍식 ,  박명순 ,  노영쇠 ,  권윤자 ,  박시호 ,  엄수종 ,  장판식 ,  조성동

IPC: C07C227/18

Abstract: PURPOSE: A novel method for preparing α-L-aspartyl-L-phenylalanine methyl ester·HCl salt is provided, to reduce the amount of by-products, to allow the by-products to be reused after hydrolysis and to increase the production yield by 20% or more. CONSTITUTION: The method comprises the steps of reacting L-aspartic acid with trimethyl silyl chloride in the presence of an alcohol solvent to esterify the β-COOH selectively to obtain α-L-aspartyl-L-phenylalanine methyl ester·HCl. Preferably the alcohol solvent is selected from the group consisting of methyl alcohol, ethyl alcohol, allyl alcohol and benzyl alcohol. Preferably the method comprises further the steps of protecting an amine group with -CHO, Boc, benzyloxycarbonyl group, or -C(O)OR¬2 (R2 is methyl. ethyl or isobutyl group) by using acetic anhydride and formic acid.

Abstract translation: 目的：提供一种制备α-L-天冬氨酰-L-苯丙氨酸甲酯盐酸盐的新方法，以减少副产物的量，使副产物在水解后重新使用和 将产量提高20％以上。 构成：该方法包括以下步骤：在醇溶剂存在下使L-天冬氨酸与三甲基甲硅烷基氯化物反应以选择性酯化β-COOH以获得α-L-天冬氨酰-L-苯丙氨酸甲酯和amp ; middot; HCl中。 优选醇溶剂选自甲醇，乙醇，烯丙醇和苯甲醇。 优选地，该方法还包括通过使用乙酸酐和甲酸，用-CHO，Boc，苄氧基羰基或-C（O）OR 2（R 2为甲基，乙基或异丁基）保护胺基的步骤。

公开(公告)号：KR1020030062126A

公开(公告)日：2003-07-23

申请号：KR1020020002561

申请日：2002-01-16

Applicant: (주)케비젠

Inventor： 엄수종 ,  장판식 ,  김소미 ,  박찬수 ,  한혜숙 ,  신원필 ,  신홍식 ,  박시호 ,  김동명

IPC: C12N15/53

CPC classification number: C12N9/0069 ,  C12P7/02 ,  C12Y113/11063

Abstract: PURPOSE: Mutant genes of human beta, beta-carotene 15, 15'-dioxygenase having improved enzyme activity are provided, so that enzyme proteins expressed from the mutant genes can be used in various fields related to the vitamin A production. CONSTITUTION: Mutant genes of human beta, beta-carotene 15, 15'-dioxygenase are produced by error-prone PCR using a human beta, beta-carotene 15, 15'-dioxygenase gene as templates, and have the enzyme activity of the expressed protein of 1100 pmol/h. A method for producing vitamin A comprises digesting beta-carotene using the expressed human beta, beta-carotene 15, 15'-dioxygenase. A method for producing vitamin A comprises transforming a host cell using the mutant genes of human beta, beta-carotene 15, 15'-dioxygenase, and culturing the transformed host cell in a medium, wherein the host cell is a plant cell, a prokaryotic cell, a yeast cell, an insect cell, a mammal cell or a poultry cell.

Abstract translation: 目的：提供具有改善的酶活性的人β，β-胡萝卜素15,15'-双加氧酶的突变基因，使得从突变基因表达的酶蛋白可用于与维生素A生产相关的各种领域。 构成：使用人β，β-胡萝卜素15,15'-双加氧酶基因作为模板，通过易错PCR产生人β，β-胡萝卜素15,15'-双加氧酶的突变基因，并具有表达的酶活性 蛋白质为1100 pmol / h。 生产维生素A的方法包括使用表达的人β，β-胡萝卜素15,15'-双加氧酶消化β-胡萝卜素。 生产维生素A的方法包括使用人β，β-胡萝卜素15,15'-双加氧酶的突变基因转化宿主细胞，并在培养基中培养转化的宿主细胞，其中宿主细胞是植物细胞，原核 细胞，酵母细胞，昆虫细胞，哺乳动物细胞或家禽细胞。