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公开(公告)号：KR100860001B1
公开(公告)日：2008-09-25
申请号：KR1020070039899
申请日：2007-04-24
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
CPC classification number: C12Q1/6806 , C12Q1/6844
Abstract: A method is provided to extract high quality DNA stably by decomposing gelatinized starch of boiled rice, thereby being utilized to identify varieties of the rice during distribution and consumption steps. A method for identifying rice varieties comprises the steps of: (a) decomposing boiled rice obtained by gelatinizing rice with heat-resistant alpha-amylase in a 2% CTAB(cetyltrimethylammonium bromide) buffer solution to extract DNA therefrom; (b) amplifying the extracted DNA through PCR using at least one SSR primer selected from the group consisting of an OSR20 primer consisting of SEQ ID : NOs. 1 and 2, an RM240 primer consisting of SEQ ID : NOs. 3 and 4; and an RM257 primer consisting of SEQ ID : NOs. 5 and 6; and (c) identifying variety from the PCR product through DNA finger printing.
Abstract translation: 提供一种通过分解煮熟米饭糊化淀粉来稳定提取高质量DNA的方法，从而用于在分配和消费步骤中鉴定水稻品种。 一种鉴定水稻品种的方法包括以下步骤：（a）将2％CTAB（十六烷基三甲基溴化铵）缓冲溶液中的耐热α-淀粉酶将米糊化得到的煮熟的水稻分解，从而提取DNA; （b）使用至少一种SSR引物通过PCR扩增提取的DNA，所述SSR引物选自由SEQ ID NO：组成的OSR20引物组成的组。 1和2，RM240引物，其由SEQ ID NO： 3和4; 和由SEQ ID：NO组成的RM257引物。 5和6; 和（c）通过DNA指纹印刷从PCR产物鉴定品种。
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公开(公告)号：KR1019990065336A
公开(公告)日：1999-08-05
申请号：KR1019980000581
申请日：1998-01-12
Applicant: 대한민국(농촌진흥청장)
IPC: A61K36/804 , A61K36/258 , A61K36/076
Abstract: 본 발명은 편이식 경옥고 제조방법 및 그 경옥고에 관한 것으로, 생지황즙액, 인삼가루, 복령가루, 꿀 등을 배합비에 따라 혼합하여 스테인레스 용기에 넣고 70∼80℃의 수욕상에서 5일간 숙성시켜 반고형 죽상태의 종래 경옥고를 제조한 후 여기에 2배량의 물을 넣고 10분간 끓인후 경옥고 용액을 압착추출하고, 이 용액에 한천, 설탕, 팟앙금 또는 한천, 설탕, 올리고당 등을 배합비에 따라 넣고 가열하여 졸임으로써 일정형상을 갖는 양갱형 또는 젤리형 경옥고를 제조함으로써, 건강기능성 식품을 선호하는 현대인들의 욕구에 부응할 수 있는 새로운 형태의 즉석 편이식 경옥고를 제공하는 한편, 약용작물 재배농가의 소득증대에도 기여할 수 있는 특징이 있다.
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公开(公告)号：KR1020010016876A
公开(公告)日：2001-03-05
申请号：KR1019990032076
申请日：1999-08-05
Applicant: 대한민국(농촌진흥청장)
IPC: A23F3/00
Abstract: PURPOSE: An instant tea product made from powdered kyoungokko, a kind of Korean traditional restorative, glucose, ginseng flavor and lactic acid is provided to promote metabolism and reactivity of body by the kyoungokko ingredient. CONSTITUTION: The instant tea is prepared by the steps of: (i) making kyoungokko by compounding 52.6wt% of Rehmanniae Radix juice, 5.3wt% of ginseng powder, 10.5w% of powdered hoelen and 31.6wt% of honey in a stainless container and aging by heating the mixture in a water bath of 70-80deg.C for 5days; (ii) boiling the blackish brown viscous kyoungokko with 2times of water for 10min and getting a solution of kyoungokko by compressing the boiled kyoungokko in a sack; (iii) freezing the resulting solution at -60 to -70deg.C for a day and drying the frozen solution in a vacuum drier under a condition of 30-40deg.C and 90-110mmHg; and then (iv) mixing 30wt% of the lyophilized kyoungokko powder with 66-66.7wt% of glucose, 0.1-3.0wt% of ginseng flavor and 2.9-3.0wt% of lactic acid. The tea composition of kyoungokko is then melted in hot water to be taken.
Abstract translation: 目的：提供由kyoungokko粉末制成的一种速溶茶产品，一种韩国传统修复剂，葡萄糖，人参风味和乳酸，以促进kyoungokko成分的身体代谢和反应性。 构成：速溶茶是通过以下步骤制备的：（i）通过在不锈钢容器中混合地塞米松52.6重量％的人参粉末，5.3重量％的人参粉末，10.5w的蜂巢粉和31.6重量％的蜂蜜，制成kyoungokko 并将混合物在70-80℃的水浴中加热5天; （ii）用2次水煮沸黑褐色粘稠的kyoungokko 10分钟，通过在一袋中压制煮沸的kyoungokko得到kyoungokko的溶液; （iii）将所得溶液在-60℃-70℃冷冻一天，并在真空干燥器中在30-40℃和90-110mmHg的条件下干燥冷冻溶液; 然后（iv）将30重量％的冻干的kyoungokko粉末与66-66.7重量％的葡萄糖，0.1-3.0重量％的人参风味和2.9-3.0重量％的乳酸混合。 然后将kyoungokko的茶组合物在热水中熔化以进行摄取。
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公开(公告)号：KR1019990065617A
公开(公告)日：1999-08-05
申请号：KR1019980001000
申请日：1998-01-15
Applicant: 대한민국(농촌진흥청장)
IPC: C12P19/14
Abstract: 본 발명은 보리, 호밀, 귀리와 같은 곡물류로부터 천연식이섬유이며 생리활성물질인 β-글루칸을 추출하는 방법에 관한 것으로, β-글루칸을 함유한 곡물분말을 가수(加水) 용해시켜 β-글루칸을 추출하고 불순물을 제거한 후 주정 또는 알코올류를 이용하여 분리농축하여, 쌀보리의 경우 100g당 순도 80∼90％의 β-글루칸을 약 3∼4g정도 수득할 수 있는 특징이 있다.
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Patent Agency Ranking
公开(公告)号：KR100354962B1
公开(公告)日：2002-10-05
申请号：KR1019990032076
申请日：1999-08-05
Applicant: 대한민국(농촌진흥청장)
IPC: A23F3/00
Abstract: 본발명은기호성이우수하고음용하기쉬운경옥고즉석차및 그제조방법에관한것으로서, 동의보감처방에의해제조된반고형죽상태의경옥고에물을혼합하여가열후 압착여과시켜경옥고용액을추출한후 추출된경옥고용액을동결시켜진공동결건조기로건조하고, 동결건조된경옥고분말에포도당, 인삼향및 젖산을배합하여즉석차를제조함으로써, 휴대및 보관이용이하고분산성이높아음용이편리한경옥고즉석차를제공하는한편, 생약재의소비를촉진하여약용작물재배농가의소득증대에도기여할수 있다.
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7.发明授权
公开(公告)号：KR100341797B1
公开(公告)日：2002-06-26
申请号：KR1019990032077
申请日：1999-08-05
Applicant: 대한민국(농촌진흥청장)
IPC: C07D209/04 , A61K31/4045
Abstract: 본발명은인체에무해하고항산화력이우수한천연항산화제를제공하기위하여홍화씨에함유되어있는항산화물질인 N-페룰로일세로토닌(N-feruloylserotonin)을분리정제하는방법에관한것으로서, 홍화씨를분쇄하여메탄올(MeOH) 용액으로항산화물질을추출하는단계와, 상기추출물을에틸아세테이트, 노르말부탄올물을및 물로순차분획하는단계와, 상기분획물중 용매를제거한에틸아세테이트분획유기용매에용해시킨후 칼럼크로마토그래피를통과시켜일정량씩분취하는단계와, 상기분취된각 분획물을박층크로마토그래피(TLC)를통과시켜전개된물질의높이가같은분획물끼리합하여유사물질끼리분취하는단계와, 상기분취된각 유사물질들의 DPPH 라디칼활성을측정하여항산화물질을추적분리하는단계를포함하여구성되어홍화씨에함유된 N-페룰로일세로토닌을간단한공정에의해분리추출할수 있으며, 크로마토그래피에의해분리된분획물의 DPPH 라디컬소거활성을측정하여활성물질을추적분리하므로간편하고신속하게고순도의정제된항산화제를얻을수 있는장점이있다.
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公开(公告)号：KR100261045B1
公开(公告)日：2000-07-01
申请号：KR1019980001000
申请日：1998-01-15
Applicant: 대한민국(농촌진흥청장)
IPC: C12P19/14
Abstract: PURPOSE: Provided is a method for simply extracting beta-glucan from grains at high yields. CONSTITUTION: The method for extracting beta-glucan comprises the steps of: grinding grains containing beta-glucan such as barley, rye or oats; adding hot water into the grain powder; agitating the grain mixture for 1 to 2 hours and centrifuging it; collecting the supernatant therefrom and heating it at 60 to 100 deg. C to deactivate beta-glucanase and coagulate proteins; adding alpha-amylase into the solution and cooling it; filtering the solution to remove coagulated proteins; adding alcohol into the filtrate and slowly agitating the solution to precipitate solid components; and centrifuging the solution and removing the supernatant to collect the solid beta-glucan.
Abstract translation: 目的：提供以高产率从谷物中简单提取β-葡聚糖的方法。 构成：提取β-葡聚糖的方法包括以下步骤：研磨含有β-葡聚糖如谷物，黑麦或燕麦的谷物; 将热水加入谷物粉末; 搅拌谷物混合物1至2小时并离心; 收集上清液并加热至60至100度。 C使β-葡聚糖酶失活并凝结蛋白质; 向溶液中加入α-淀粉酶并冷却; 过滤溶液以去除凝固的蛋白质; 向滤液中加入酒精并缓慢搅拌溶液以沉淀固体组分; 并离心溶液并除去上清液以收集固体β-葡聚糖。
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公开(公告)号：KR100439318B1
公开(公告)日：2004-07-07
申请号：KR1020010075954
申请日：2001-12-03
Applicant: 대한민국(농촌진흥청장) , 주식회사 한일씨에프에스
IPC: A23B7/02
Abstract: PURPOSE: A precooling apparatus capable of shortening a precooling time is provided which reduces labor power and consumption energy for the precooling process and preserves high quality vegetables or fruits for a long period of time. CONSTITUTION: In a tunnel type precooling apparatus, an inlet door(1) and outlet door, which are slid, opened and closed up and downwardly, are installed on both sides of a precooling chamber(3), a transfer conveyor(3a) for transferring each precooling product is installed in plural rows inside the inlet and outlet door and at the interior of the precooling chamber. A cold air through-hole(8a) also is formed at the interior of the cooling chamber to cause the circulation of cold air between the upper and lower space. A differential pressure fan(4) for forcibly circulating cold air is arranged on both sides of the transfer conveyor. A unit cooler(5) forcibly circulating cold air and generating cold air is arranged at the lower part of the transfer conveyor.
Abstract translation: 目的：提供一种能够缩短预冷时间的预冷装置，该预冷装置降低了预冷过程中的劳动力和消耗能量，并且长时间保存优质的蔬菜或水果。 本发明提供一种隧道式预冷却装置，在预冷室（3）的两侧安装上下滑动开闭的入口门（1）和出口门（3），传送带（3a） 每个预冷产品的传输在入口和出口门的内部以及预冷室的内部安装成多排。 冷空气通孔（8a）也形成在冷却室的内部，以引起上部空间和下部空间之间的冷空气循环。 传送输送机的两侧设有强制循环冷气的差压风机（4）。 在传送输送机的下部设有单元冷却器（5），用于强制循环冷空气并产生冷空气。
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公开(公告)号：KR1020030045327A
公开(公告)日：2003-06-11
申请号：KR1020010075954
申请日：2001-12-03
Applicant: 대한민국(농촌진흥청장) , 주식회사 한일씨에프에스
IPC: A23B7/02
Abstract: PURPOSE: A precooling apparatus capable of shortening a precooling time is provided which reduces labor power and consumption energy for the precooling process and preserves high quality vegetables or fruits for a long period of time. CONSTITUTION: In a tunnel type precooling apparatus, an inlet door(1) and outlet door, which are slid, opened and closed up and downwardly, are installed on both sides of a precooling chamber(3), a transfer conveyor(3a) for transferring each precooling product is installed in plural rows inside the inlet and outlet door and at the interior of the precooling chamber. A cold air through-hole(8a) also is formed at the interior of the cooling chamber to cause the circulation of cold air between the upper and lower space. A differential pressure fan(4) for forcibly circulating cold air is arranged on both sides of the transfer conveyor. A unit cooler(5) forcibly circulating cold air and generating cold air is arranged at the lower part of the transfer conveyor.
Abstract translation: 目的：提供能够缩短预冷时间的预冷却装置，其能够降低预冷工艺的劳动力和消耗能量，并且能够长时间保持高质量的蔬菜或水果。 构成：在隧道式预冷装置中，在预冷室（3）的两侧安装入口门（1）和出口门，所述入口门（1）和出口门被上下滑动，打开和关闭，传送输送机（3a）用于 传送每个预冷却产品安装在入口和出口门内部以及预冷室内部的多排中。 冷空气通孔（8a）也形成在冷却室的内部，以使冷空气在上部和下部空间之间循环。 用于强制循环冷气的差压风扇（4）布置在输送机的两侧。 在输送机的下部配置有强制循环冷气并产生冷空气的单元冷却器（5）。
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