Abstract:
PURPOSE: A non-destructive analysis method for measuring protein contents of brown and milled rice of wet-paddy rice by a near infrared spectroscopy is provided to simply evaluate the wet-paddy rice by analyzing and predicting the protein content of brown and milled rice. CONSTITUTION: A non-destructive analysis method for measuring protein contents of brown and milled rice of wet-paddy rice by a near infrared spectroscopy comprises the following steps. A near-infrared spectrum is obtained from test sample wet-paddy rice. The polished rice is made by polishing the test sample wet-paddy rice and the protein content of the polished rice is measured. A measurement formula is derived using obtained correlation of the near-infrared spectrum and the protein content of the polished rice. The near-infrared spectrum is obtained from the sample wet-paddy rice. The protein content of the polished rice is predicted using the measuring formula.
Abstract:
A method is provided to extract high quality DNA stably by decomposing gelatinized starch of boiled rice, thereby being utilized to identify varieties of the rice during distribution and consumption steps. A method for identifying rice varieties comprises the steps of: (a) decomposing boiled rice obtained by gelatinizing rice with heat-resistant alpha-amylase in a 2% CTAB(cetyltrimethylammonium bromide) buffer solution to extract DNA therefrom; (b) amplifying the extracted DNA through PCR using at least one SSR primer selected from the group consisting of an OSR20 primer consisting of SEQ ID : NOs. 1 and 2, an RM240 primer consisting of SEQ ID : NOs. 3 and 4; and an RM257 primer consisting of SEQ ID : NOs. 5 and 6; and (c) identifying variety from the PCR product through DNA finger printing.
Abstract translation:提供一种通过分解煮熟米饭糊化淀粉来稳定提取高质量DNA的方法,从而用于在分配和消费步骤中鉴定水稻品种。 一种鉴定水稻品种的方法包括以下步骤:(a)将2%CTAB(十六烷基三甲基溴化铵)缓冲溶液中的耐热α-淀粉酶将米糊化得到的煮熟的水稻分解,从而提取DNA; (b)使用至少一种SSR引物通过PCR扩增提取的DNA,所述SSR引物选自由SEQ ID NO:组成的OSR20引物组成的组。 1和2,RM240引物,其由SEQ ID NO: 3和4; 和由SEQ ID:NO组成的RM257引物。 5和6; 和(c)通过DNA指纹印刷从PCR产物鉴定品种。