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公开(公告)号：KR100423121B1
公开(公告)日：2004-03-18
申请号：KR1020000081428
申请日：2000-12-23
IPC: C12N1/20
Abstract: PURPOSE: Bacillus amyloliquefaciens CHO104 with antagonistic property and an antimicrobial compound produced thereby are provided. The novel antimicrobial compound can be useful in medicines, agricultural chemicals, foods, etc. CONSTITUTION: The microorganism Bacillus amyloliquefaciens CHO104(KCTC 18060P) isolated from the soil produces an antimicrobial compound. A microbial preparation containing Bacillus amyloliquefaciens CHO104 is prepared by culturing Bacillus amyloliquefaciens CHO104 in SDBP(soy dextrose beef ex. proteose peptone) medium at 30 to 35 deg. C. The novel antimicrobial compound, Amyloliquetone, is produced by the steps of: separating the supernatant of the cultured medium of Bacillus amyloliquefaciens CHO104; collecting an ethylacetate-soluble acidic·neutral fraction from the supernatant by adding 1N HCl; collecting an ethylacetate-soluble neutral fraction by adding 2 to 5% NaHCO3; subjecting the ethylacetate-soluble neutral fraction to an adsorption chromatography on silica gel to obtain an active fraction; and subjecting the active fraction to octadecylsilane column chromatography and HPLC, sequentially.
Abstract translation: 目的：提供具有拮抗特性的解淀粉芽孢杆菌CHO104和由此产生的抗微生物化合物。 该新型抗微生物化合物可用于医药，农业化学品，食品等。组成：从土壤中分离的微生物解淀粉芽孢杆菌CHO104（KCTC 18060P）产生抗微生物化合物。 含有解淀粉芽孢杆菌CHO104的微生物制剂通过在30至35℃下在SDBP（大豆葡萄糖牛奶，蛋白胨，大豆蛋白）培养基中培养解淀粉芽孢杆菌CHO104来制备。 C.新型抗微生物化合物淀粉水解酮通过以下步骤制备：分离解淀粉芽孢杆菌CHO104培养基的上清液; 通过加入1N HCl从上清液中收集可溶于乙酸乙酯的酸性＆amp;中性部分; 通过加入2至5％的NaHCO 3收集乙酸乙酯可溶的中性部分; 使乙酸乙酯可溶的中性级分在硅胶上进行吸附层析以获得活性级分; 并依次将活性部分进行十八烷基硅烷柱层析和HPLC。
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公开(公告)号：KR1020020051803A
公开(公告)日：2002-06-29
申请号：KR1020000081428
申请日：2000-12-23
IPC: C12N1/20
Abstract: PURPOSE: Bacillus amyloliquefaciens CHO104 with antagonistic property and an antimicrobial compound produced thereby are provided. The novel antimicrobial compound can be useful in medicines, agricultural chemicals, foods, etc. CONSTITUTION: The microorganism Bacillus amyloliquefaciens CHO104(KCTC 18060P) isolated from the soil produces an antimicrobial compound. A microbial preparation containing Bacillus amyloliquefaciens CHO104 is prepared by culturing Bacillus amyloliquefaciens CHO104 in SDBP(soy dextrose beef ex. proteose peptone) medium at 30 to 35 deg. C. The novel antimicrobial compound, Amyloliquetone, is produced by the steps of: separating the supernatant of the cultured medium of Bacillus amyloliquefaciens CHO104; collecting an ethylacetate-soluble acidic·neutral fraction from the supernatant by adding 1N HCl; collecting an ethylacetate-soluble neutral fraction by adding 2 to 5% NaHCO3; subjecting the ethylacetate-soluble neutral fraction to an adsorption chromatography on silica gel to obtain an active fraction; and subjecting the active fraction to octadecylsilane column chromatography and HPLC, sequentially.
Abstract translation: 目的：提供具有拮抗性的解淀粉芽孢杆菌CHO104和由此产生的抗微生物化合物。 新型抗菌化合物可用于药物，农药，食品等。构成：从土壤中分离的微生物解淀粉芽孢杆菌CHO104（KCTC 18060P）产生抗微生物化合物。 含有解淀粉芽孢杆菌CHO104的微生物制剂通过在30-35℃下在SDBP（大豆葡萄糖牛肉例如蛋白胨）中培养解淀粉芽孢杆菌CHO104来制备。 C.新型抗微生物化合物淀粉木粉是通过以下步骤制备的：分离解淀粉芽孢杆菌CHO104的培养基培养基的上清液; 通过加入1N HCl从上清液中收集乙酸乙酯可溶的酸性中性级分; 通过加入2至5％的NaHCO 3收集乙酸乙酯可溶的中性馏分; 使乙酸乙酯可溶性中性部分在硅胶上进行吸附层析，得到活性部分; 并依次将活性级分进行十八烷基硅烷柱色谱和HPLC。
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公开(公告)号：KR100525886B1
公开(公告)日：2005-11-02
申请号：KR1020040011176
申请日：2004-02-16
Applicant: 박근형
IPC: C07H1/08
Abstract: 본 발명은 지초(
Lithospermum erythrorhizon ) 뿌리의 에탄올추출물에 1-메틸-미오-이노시톨, 수크로오스, 1-케스토스, 니스토스등의 기능성 당화합물 존재확인 및 이들 물질의 분리방법에 관한 것이다.
지초뿌리를 에탄올 용매로 추출하여 얻어진 에탄올추출물을 농축하여 에탄올을 제거하고, 이어서 헥산과 에틸아세테이트로 세척한 다음, 메탄올로 용해하여 메탄올가용물을 얻은 후, 이 메탄올가용물을 실리카켈 흡착 컬럼 크로마토그라피, 옥타데실실란 컬럼 크로마토그라피, 세파덱스 LH-20 컬럼 크로마토그라피, GPC-HPLC등의 단계를 거쳐 최종적으로 4종의 화합물을 단리하였으며, FAB-MS, NMR 분석에 의해 상기 네가지 물질로 구조 결정하여, 이들 당화합물이 지초뿌리에 함유되어있음을 밝히고, 이들 당화합물의 분리방법을 제공한다.-
公开(公告)号：KR1020050081809A
公开(公告)日：2005-08-19
申请号：KR1020040011176
申请日：2004-02-16
Applicant: 박근형
IPC: C07H1/08
Abstract: 본 발명은 지초(
Lithospermum erythrorhizon ) 뿌리의 에탄올추출물에 1-메틸-미오-이노시톨, 수크로오스, 1-케스토스, 니스토스등의 기능성 당화합물 존재확인 및 이들 물질의 분리방법에 관한 것이다.
지초뿌리를 에탄올 용매로 추출하여 얻어진 에탄올추출물을 농축하여 에탄올을 제거하고, 이어서 헥산과 에틸아세테이트로 세척한 다음, 메탄올로 용해하여 메탄올가용물을 얻은 후, 이 메탄올가용물을 실리카켈 흡착 컬럼 크로마토그라피, 옥타데실실란 컬럼 크로마토그라피, 세파덱스 LH-20 컬럼 크로마토그라피, GPC-HPLC등의 단계를 거쳐 최종적으로 4종의 화합물을 단리하였으며, FAB-MS, NMR 분석에 의해 상기 네가지 물질로 구조 결정하여, 이들 당화합물이 지초뿌리에 함유되어있음을 밝히고, 이들 당화합물의 분리방법을 제공한다.-
公开(公告)号：KR1020020043131A
公开(公告)日：2002-06-08
申请号：KR1020000072613
申请日：2000-12-01
Applicant: 박근형
IPC: C07D213/62
Abstract: PURPOSE: A novel natural antimicrobial substance isolated from the stalk and leaf of Hovenia dulcis Thunb and an isolating method thereof are provided. Therefore, the novel natural antimicrobial substance, hovenidine B6, having pyridine structure can be found. CONSTITUTION: The novel natural antimicrobial substance, hovenidine B6 is isolated from the stalk and leaf of Hovenia dulcis Thunb by the steps of: heat-extracting the stalk and leaf of Hovenia dulcis Thunb; centrifuging the extract and freeze-drying the supernatant to obtain the extract powder; concentrating the methanol filtrate under reduced pressure to obtain methanol extract; adding a weak alkali buffer and ethylacetate into the methanol extract to divide a liquid fraction and an ethylacetate neutral fraction, and adding ethylacetate into the liquid fraction to obtain ethylacetate acid fraction; subjecting the above fractions to silica gel adsorption column chromatography using ethylacetate-methanol solution to obtain an active fraction; subjecting the active fraction to gel filtration chromatography using Sephadex LH-20 column; and subjecting the purified fraction to reverse HPLC using methanol-water solvent to isolate hovenidine B6.
Abstract translation: 目的：提供从枸杞子的茎和叶分离的新型天然抗菌物质及其分离方法。 因此，可以发现具有吡啶结构的新型天然抗菌物质赫非啶B6。 构成：通过以下步骤从枸杞子的茎和叶中分离出新颖的天然抗菌物质贝伐单菌B6：热提取枸杞子的茎和叶; 离心提取物并冷冻干燥上清液，得到提取物粉末; 减压浓缩甲醇滤液得甲醇萃取液; 在甲醇提取液中加入弱碱性缓冲液和乙酸乙酯，分离出液体馏分和乙酸乙酯中性馏分，并向乙酸乙酯中加入乙酸乙酯，得到乙酸乙酯酸馏分; 将上述级分用乙酸乙酯 - 甲醇溶液进行硅胶吸附柱层析，得到活性部分; 使用Sephadex LH-20柱对活性部分进行凝胶过滤色谱; 并使用甲醇 - 水溶剂对纯化的级分进行反相HPLC，以分离出霍尼定B6。
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Patent Agency Ranking6.发明公开항산화 활성을 갖는 루테올린 7-O-β-D-글루코피라노시드,퀘서틴 3-O-β-D-갈락토피라노시드, 퀘서틴3-O-β-D-글루코피라노시드, 퀘서틴3-O-α-D-아라비노피라노시드, 캠펠로3-O-α-D-아라비노피라노시드, 루테올린3-O-β-D-람노피라노실(1→6)-7-O-α-L-글루코피라노시드등의 천연 항산화물질을 포함하여 항산화기능을 갖는신선초조제물 및 이의제조방법 无效含有INTEOLIN 7-O-β-D-吡喃葡萄糖苷，3-O-β-D-吡喃葡萄糖苷，3-O-β-D-吡喃半乳糖苷，3-O-α-D-吡喃半乳糖苷奎宁酯的抗氧化功能的ANGELICA KEISKEI制备 ，KAEMPFEROL 3-O-α-D-阿拉伯糖苷，LUTEOLIN 3-O-β-D-RHAMNOPYRANOSYL（1-> 6）-7-O-α-L-吡喃葡萄糖苷及其制备方法
公开(公告)号：KR1020050081810A
公开(公告)日：2005-08-19
申请号：KR1020040011177
申请日：2004-02-16
Applicant: 박근형
IPC: A61K31/7048
CPC classification number: A61K31/7048 , A61K9/14 , A61K36/232 , A61K2236/55
Abstract: 본 발명은 천연항산화물질을 포함한 항산화 기능을 갖는 신선초조제물 및 제조에 관한 것이다. 보다 상세하게는 신선초를 열수로 추출한 추출물을 용매분획하여 얻어진 에칠아세테이트가용중성획분을 세파덱스 LH-20 컬럼 크로마토그라피 및 HPLC를 의해 루테올린 7-
O -β-D-글루코피라노시드, 퀘서틴 3-
O -β-D-갈락토피라노시드, 퀘서틴 3-
O -β-D-글루코피라노시드, 퀘서틴 3-
O -α-D-아라비노피라노시드, 캠펠로 3-
O -α-D-아라비노피라노시드, 루테올린 3-
O -β-D-람노피라노실(1→6)-7-
O -α-L-글루코피라노시드를 분리하고 이들 물질의 구조결정은 MS, NMR 분석에 의해 이루어졌다. 이들 물질들은 DPPH 자유라디칼소거에 대한 항산화 활성을 갖고 있음이 검정되어, 이들 천연항산화물질을 포함한 항산화 기능을 갖는 신선초조제물은 산화에 의한 질병예방 및 노화억제효력을 갖는 기능성소재로서의 용도를 제공한다.-
7.发明公开차엽으로부터 카페인, 에피갈로카테킨, 에피카테킨,에피갈로카테킨갈레이트, 에피카테킨갈레이트의 분리 및제조방법 无效椰子油，EPIGOCOCCHIN，EPICATECHIN，EPIGOCOCOCCHINGALLATE和茶叶叶片的分离和生产方法
公开(公告)号：KR1020030060723A
公开(公告)日：2003-07-16
申请号：KR1020020001836
申请日：2002-01-09
Applicant: 박근형
IPC: C07D311/62
Abstract: PURPOSE: Separation and production methods of caffeine, epigallocatechin, epicatechin, epigallocatechingallate, and epicatechingallate from tea leaves are provided, thereby simultaneously separating and producing the five kinds of materials. CONSTITUTION: Separation and production methods of caffeine, epigallocatechin, epicatechin, epigallocatechingallate, and epicatechingallate from tea leaves comprises the steps of: steaming tea leaves and drying them; heat-extracting the dried tea leaves or extracting the extract thereof with EtOH and freeze-drying it; subjecting the powdery extract to the sephadex column to obtain fractions of caffeine, epigallocatechin, epicatechin, epigallocatechingallate, and epicatechingallate; and subjecting the fractions to HPLC, removing an organic solvent and freeze-drying the HPLC fractions.
Abstract translation: 目的：提供茶叶中咖啡因，表没食子儿茶素，表儿茶素，表没食子儿茶素和表儿茶酸的分离和制备方法，同时分离和生产五种材料。 构成：茶叶中的咖啡因，表没食子儿茶素，表儿茶素，表没食子儿茶酸和表儿茶酸的分离和制备方法包括以下步骤：蒸煮茶叶并干燥; 用干燥的茶叶进行热提取或用EtOH萃取其提取物并冷冻干燥; 将粉末状提取物经过sephadex柱，得到咖啡因，表儿茶素，表儿茶素，表没食子儿茶素和表儿酸酯的部分; 并将级分进行HPLC，除去有机溶剂并冷冻干燥HPLC级分。
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公开(公告)号：KR1020010077323A
公开(公告)日：2001-08-17
申请号：KR1020000005033
申请日：2000-02-02
Applicant: 박근형
IPC: A23F3/00
Abstract: PURPOSE: A method for preparing a tea using the trunk and leaves of Hovenia dulcis and preparing a granule tea and a drink using their hot-water extract is provided. Thereby, the trunk and leaves of Hovenia dulcis is utilized well, which has fluent minerals, nutrition, effects to remove alcohol poisoning, and antibacterial and antioxidant effects. CONSTITUTION: A method for preparing a tea using the trunk and leaves of Hovenia dulcis comprises the steps of: (i) collecting the trunk and leaves of Hovenia dulcis from middle June to late July; (ii) heating with hot steam of 0.2kg/cm¬2 and 95deg.C for 20-45 minutes to inactivate the enzyme of leaves and trunk; (iii) cooling with cool air; (iv) destructing the leaves and trunk and drawing out water from them with a related device; (v) drying with a hot air dryer at 50-60deg.C for 10hours, and then pulverizing in 2mm size or less; and (vi) packing in tea bags. The prepared tea can be made into a granule tea and a drink using its hot-water extract.
Abstract translation: 目的：提供使用枸杞子的树干和叶子制备茶的方法，并使用其热水提取物制备颗粒茶和饮料。 因此，枸杞子的树干和叶片利用效果好，具有流动的矿物质，营养，去除酒精中毒的作用，抗菌和抗氧化作用。 构成：使用枸杞子的树干和叶子制备茶的方法包括以下步骤：（i）从6月中旬至7月下旬收集枸杞子的树干和叶子; （ii）用0.2kg / cm 2和95℃的热蒸汽加热20-45分钟以灭活叶和树干的酶; （iii）用冷空气冷却; （iv）破坏叶子和树干，用相关装置从中抽出水; （v）用50-60℃的热空气干燥器干燥10小时，然后粉碎至2mm或更小; 和（vi）包装在茶包中。 使用其热水提取物制成的茶可制成颗粒茶和饮料。
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公开(公告)号：KR100482933B1
公开(公告)日：2005-04-14
申请号：KR1020020077531
申请日：2002-12-04
Applicant: 박근형
IPC: G01N33/14
Abstract: 본 발명은 진도홍주색소의 감별 방법에 관한 것으로, 지초를 사용하여 제조된 진도홍주를 분액여두를 이용하여 에칠아세테이트계 용매계로 분배하면 진도홍주의 홍색소가 이들 유기용매계로 분배되어지는 성질을 이용하여 감별하는 1단계 감별방법, 분광광도계의 의한 진도홍주색소의 분광학적 성질을 이용하여 감별하는 2단계 감별방법, 그리고 옥타데실실란 컬럼이 장착된 고성능액체크로마토그라피에 의해 진도홍주 홍색소 용출양상을 검토하여 감별하는 3단계 감별방법으로 구성된 진도홍주색소의 1, 2, 3단계 감별방법에 의해 진도홍주 홍색소가 지초유래의 색소인지를 간편하고도 정확하게 식별할 수 있는 감별방법을 제공한다.
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10.发明授权
公开(公告)号：KR100439012B1
公开(公告)日：2004-07-03
申请号：KR1020010017769
申请日：2001-03-29
Applicant: 박근형
Abstract: PURPOSE: A natural antioxidant substance containing 4-hydroxy-3-methoxy benzoic acid, 4-hydroxy-3-methoxy cinnamic acid, quercetin-3-0-β-D-glucopyranoside and hovenidine B6 as a new natural antioxidant active material separated from Hovenia dulcis Thunb. is provided which is expected to be used as a material of food and medicine having an anti-aging function. CONSTITUTION: Leaves and stems of Hovenia dulcis Thunb. are extracted in water, freeze dried and then extracted in methanol, followed by solvent fractionation to produce an ethyl acetate acidic fraction and neural fraction. An active material contained in the extract is then purified with a purifying means such as silica gel column chromatography, chromatography with Sephadex LH-20, octadecylsilane column chromatography, a reversed-phase high-performance liquid chromatography or the like.
Abstract translation: 用途：含有4-羟基-3-甲氧基苯甲酸，4-羟基-3-甲氧基肉桂酸，槲皮素-3-O-β-D-吡喃葡萄糖苷和hovenidine B6作为新的天然抗氧化剂活性物质的天然抗氧化剂物质 从Hovenia dulcis Thunb分离的材料。 预计将被用作具有抗衰老功能的食物和药物的材料。 组成：Hovenia dulcis Thunb的叶和茎。 在水中萃取，冷冻干燥，然后用甲醇萃取，随后溶剂分馏以产生乙酸乙酯酸性部分和神经部分。 然后提取物中所含的活性物质用硅胶柱色谱等纯化手段，Sephadex LH-20色谱法，十八烷基硅烷柱色谱法，反相高效液相色谱法等纯化。
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