고온성 절대 공생 미생물 심비오박테리움의 공생균주인 고온성 미생물 바실러스속 균주 유래의 내열성 글루타메이트라세마아제를 암호하는 유전자 및 이를 이용한 내열성 글루타메이트라세마아제의 제조방법

    公开(公告)号:KR100251523B1

    公开(公告)日:2000-04-15

    申请号:KR1019970060212

    申请日:1997-11-15

    Abstract: PURPOSE: Provided is a gene coding thermostable glutamateracemase(GluRA) originated from a thermophile Bacillus sp. strain, a symbiotic strain of thermophilic absolute symbiont, a Symbiobacterium. And a preparation method of thermostable glutamateracemase is also provided. The glutamateracemase has excellent activity and enzymatic stability at high temperature, and is thus useful as biological catalyst in preparation of D-glutamic acid. CONSTITUTION: A gene of glutamateracemase(GluRA) originated from a thermophile Bacillus sp. strain has base sequence represented by the sequence 1. The method for preparation of thermostable glutamateracemase is composed of the following steps of: (a) culturing microbes transformed with the expression vector pGSK23 to express GluRA; (b) heating stem cell eluate obtained from the expressed GluRA; and (c) separation GluRA by non ion exchange chromatography and nonhydrophilic chromatography.

    Abstract translation: 目的:提供源自嗜热芽孢杆菌属的热稳定性谷氨酰胺酶(GluRA)的基因。 菌株,嗜热绝对共生体的共生菌株,共生杆菌。 还提供了一种耐热谷氨酰胺酶的制备方法。 谷氨酰胺酶在高温下具有优异的活性和酶稳定性,因此可用作制备D-谷氨酸的生物催化剂。 构成:源自嗜热杆菌芽孢杆菌的谷氨酰胺酶(GluRA)基因。 菌株具有由序列1表示的碱基序列。制备耐热谷氨酰胺酶的方法由以下步骤组成:(a)培养用表达载体pGSK23转化的微生物表达GluRA; (b)加热从表达的GluRA获得的干细胞洗脱液; 和(c)通过非离子交换层析和非亲水层析分离GluRA。

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