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    HBV의 S표면항원에 결합하는 생쥐 항체의 가변영역을 암호화하는 cDNA 및 이를 포함하는 재조합 플라스미드
    4.
    发明授权
    HBV의 S표면항원에 결합하는 생쥐 항체의 가변영역을 암호화하는 cDNA 및 이를 포함하는 재조합 플라스미드 失效
    用于HBV抗体的重组DNA

    公开(公告)号：KR1019960001817B1

    公开(公告)日：1996-02-05

    申请号：KR1019920011992

    申请日：1992-07-06

    Applicant: 한국과학기술연구원

    Inventor： 한문희 ,  홍효정 ,  류춘제 ,  진병래 ,  정홍근

    IPC: C12N15/13

    Abstract: The cloning method for chimeric antibody to S surface antigen of Hepatitis B virus subtype (I) consists of (1) determining N-terminal amino acid sequences of purified murine antibody to (I) with H67 cell line, (2) PCR reacting cDNA from H67 with primers for heavy chain as 5'-CCC GAA TTC GAG GT(TC) CAG CTG CA(GA) CA(GA) TCT GG(GA) TCT GG(GA) CCT G-3' and 5-CGG TCG ACA GGG ATC CAG AGT TCC AGG GTC AC-3' to get plasmid pMHV-S (KCTC 0044BP), (3) same as (2) for light chain as 5'-CCC GAA TTC GAC ATT GTG CTG AC(CA) CA(GA) TCT CC(TA) GCT-3' and 5'-CCC GCC TTG GAC ATT GTG CTG AC(CA) CA(GA) TCT CC(TA) GCT-3' to get plasmid pMKV-S (KCTC 0045BP), and (4) sequencing the genes cloned.

    Abstract translation: 乙型肝炎病毒亚型（I）的S表面抗原嵌合抗体的克隆方法包括：（1）用H67细胞系确定（I）纯化的鼠抗体的N-末端氨基酸序列，（2）PCR反应来自 H67与重链引物作为5'-CCC GAA TTC GAG GT（TC）CAG CTG C​​A（GA）CA（GA）TCT GG（GA）TCT GG（GA）CCT G-3'和5-CGG TCG ACA GGG ATC CAG AGT TCC AGG GTC AC-3'获得质粒pMHV-S（KCTC 0044BP），（3​​）与（2）相同的轻链作为5'-CCC GAA TTC GAC ATT GTG CTG AC（CA）CA ）TCT CC（TA）GCT-3'和5'-CCC GCC TTG GAC ATT GTG CTG AC（CA）CA（GA）TCT CC（TA）GCT-3'获得质粒pMKV-S（KCTC 0045BP） （4）测序克隆的基因。

    헤파타이티스비바이러스의PRE-S2표면항원에대한생쥐항체를암호하는유전자들
    6.
    发明授权
    헤파타이티스비바이러스의PRE-S2표면항원에대한생쥐항체를암호하는유전자들 失效
    HBV PRE-S2抗体基因

    公开(公告)号：KR1019950004016B1

    公开(公告)日：1995-04-22

    申请号：KR1019920001692

    申请日：1992-02-06

    Applicant: 한국과학기술연구원

    Inventor： 한문희 ,  홍효정 ,  김지영 ,  류춘제 ,  박성섭 ,  남궁욱 ,  정홍근

    IPC: C12N15/51

    Abstract: A rat antibody encoding gene to pre-S2 surface antibody of hepatitis B virus having immuno-globulin gene is separated from H8 hydridoma cell producing rat monoclonal antibody to ad sub-type pre-S2. The chimeric antibody to pre-S2 antigen of hepatitis B virus Adr type/consists of producing a heavy chain from plasmid pMHG-S2 (KCTC 0029BP) and a light chain from plasmid pMKg-S2 (KCTC 0030BP).

    Abstract translation: 将具有免疫球蛋白基因的具有免疫球蛋白基因的乙型肝炎病毒前S2表面抗体的大鼠抗体编码基因从产生H8的水稻细胞产生的大鼠单克隆抗体与ad子型前S2分离。 对乙型肝炎病毒Adr型pre-S2抗原的嵌合抗体/由质粒pMHG-S2（KCTC 0029BP）产生重链和来自质粒pMKg-S2（KCTC 0030BP）的轻链）组成。

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