公开(公告)号：CA1301646C

公开(公告)日：1992-05-26

申请号：CA541923

申请日：1987-07-13

Applicant: ABBOTT LAB

Inventor： PRY TERRY A ,  GRANADOS EDWARD N

IPC: G01N33/543 ,  G01N33/538

Abstract: A method for performing a diagnostic immunoassay by a solid phase separation. To a reaction mixture of a test sample and labeled antibody, which forms a complex of any analyte present in the test sample, is added a solid phase material having a compound capable of binding any excess labeled antibody. The solid phase material is chosen to rapidly settle whereby a solid and liquid phase is formed. The liquid phase can then be extracted to measure the amount of analyte-labeled antibody present therein. (53-2068g)

公开(公告)号：AT223053T

公开(公告)日：2002-09-15

申请号：AT97926857

申请日：1997-06-03

Applicant: ABBOTT LAB

Inventor： POPE MARK R ,  TARCHA PETER J ,  MEES DAVID R ,  JOSEPH MARY K ,  PRY TERRY A ,  PUTMAN C BRENT ,  SUBOTICH DANIEL T

IPC: G01N33/543 ,  G01N33/547 ,  G01N33/545 ,  G01N33/546

公开(公告)号：DE69016813D1

公开(公告)日：1995-03-23

申请号：DE69016813

申请日：1990-04-06

Applicant: ABBOTT LAB

Inventor： AUNET DIANE L ,  ELMORE KRISTIN D ,  GEORGEVICH GRADIMIR G ,  OOSTA GARY M ,  SIEGEL NEAL A ,  JENG TZYY-WEN ,  PRY TERRY A ,  SILVERMAN CATHY R

IPC: G01N33/48 ,  B01D39/00 ,  B01D39/16 ,  B01D39/18 ,  B01D39/20 ,  B01L3/00 ,  C12M1/12 ,  C12M1/34 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q1/44 ,  C12Q1/48 ,  G01N33/49 ,  G01N33/52 ,  G01N33/53

Abstract: A device and method for permitting the separation of plasma or serum from whole blood. The device comprises a matrix of hydrophilic sintered porous material to which at least one red blood cell agglutinating agent has been applied. According to a first method of using the device, a sample of whole blood is applied to a first end of the matrix and the red blood cells within the sample come in contact with the agglutinating agents present in the matrix. The blood cells agglutinate, and are entrapped in the interstices of the matrix, while substantially blood-cell-free serum or plasma accumulates near the outlet of the device. A filter means in liquid receiving relationship with the matrix functions to wick the serum or plasma from the matrix. According to an alternative aspect of the invention, a filter means in liquid receiving relationship with the outlet of the matrix functions to retain any blood cells which pass through the matrix as the filter means wicks the plasma or serum from the matrix. Additional agglutinating agents may be incorporated within the filter means to facilitate retention of blood cells which pass through the matrix. Another aspect of the present invention involves a device for the measurement of a serum or plasma sample, utilizing a matrix of sintered porous material, such as sintered glass, sintered steel, sintered ceramics, sintered plastics, and eguivalents thereof. The matrix is characterized by a reproducible fluid uptake capacity proportional to the fixed dimensions of said matrix, a minimal reactivity with plasma or serum components, and a hydrophilic internal surface which enables the matrix to collect and retain a predetermined volume of sample for analysis. A further aspect of the present invention provides a porous reagent delivery system comprising a matrix of sintered porous material containing at least one reagent in a freeze-dried state, and method for making same. The system allows the delivery of a unitized form of a freeze-dried reagent which is recoverable upon contact with a liquid.

公开(公告)号：AT118615T

公开(公告)日：1995-03-15

申请号：AT90106605

申请日：1990-04-06

Applicant: ABBOTT LAB

Inventor： AUNET DIANE L ,  ELMORE KRISTIN D ,  GEORGEVICH GRADIMIR G ,  OOSTA GARY M ,  SIEGEL NEAL A ,  JENG TZYY-WEN ,  PRY TERRY A ,  SILVERMAN CATHY R

IPC: G01N33/48 ,  B01D39/00 ,  B01D39/16 ,  B01D39/18 ,  B01D39/20 ,  B01L3/00 ,  C12M1/12 ,  C12M1/34 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q1/44 ,  C12Q1/48 ,  G01N33/49 ,  G01N33/52 ,  G01N33/53

Abstract: A device and method for permitting the separation of plasma or serum from whole blood. The device comprises a matrix of hydrophilic sintered porous material to which at least one red blood cell agglutinating agent has been applied. According to a first method of using the device, a sample of whole blood is applied to a first end of the matrix and the red blood cells within the sample come in contact with the agglutinating agents present in the matrix. The blood cells agglutinate, and are entrapped in the interstices of the matrix, while substantially blood-cell-free serum or plasma accumulates near the outlet of the device. A filter means in liquid receiving relationship with the matrix functions to wick the serum or plasma from the matrix. According to an alternative aspect of the invention, a filter means in liquid receiving relationship with the outlet of the matrix functions to retain any blood cells which pass through the matrix as the filter means wicks the plasma or serum from the matrix. Additional agglutinating agents may be incorporated within the filter means to facilitate retention of blood cells which pass through the matrix. Another aspect of the present invention involves a device for the measurement of a serum or plasma sample, utilizing a matrix of sintered porous material, such as sintered glass, sintered steel, sintered ceramics, sintered plastics, and eguivalents thereof. The matrix is characterized by a reproducible fluid uptake capacity proportional to the fixed dimensions of said matrix, a minimal reactivity with plasma or serum components, and a hydrophilic internal surface which enables the matrix to collect and retain a predetermined volume of sample for analysis. A further aspect of the present invention provides a porous reagent delivery system comprising a matrix of sintered porous material containing at least one reagent in a freeze-dried state, and method for making same. The system allows the delivery of a unitized form of a freeze-dried reagent which is recoverable upon contact with a liquid.

公开(公告)号：ES2185024T3

公开(公告)日：2003-04-16

申请号：ES97926857

申请日：1997-06-03

Applicant: ABBOTT LAB

Inventor： POPE MARK R ,  TARCHA PETER J ,  MEES DAVID R ,  JOSEPH MARY K ,  PRY TERRY A ,  PUTMAN C BRENT

IPC: G01N33/543 ,  G01N33/547 ,  G01N33/545 ,  G01N33/546

公开(公告)号：ES2070942T3

公开(公告)日：1995-06-16

申请号：ES90106605

申请日：1990-04-06

Applicant: ABBOTT LAB

Inventor： AUNET DIANE L ,  ELMORE KRISTIN D ,  GEORGEVICH GRADIMIR G ,  OOSTA GARY M ,  SIEGEL NEAL A ,  JENG TZYY-WEN ,  PRY TERRY A ,  SILVERMAN CATHY R

IPC: G01N33/48 ,  B01D39/00 ,  B01D39/16 ,  B01D39/18 ,  B01D39/20 ,  B01L3/00 ,  C12M1/12 ,  C12M1/34 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q1/44 ,  C12Q1/48 ,  G01N33/49 ,  G01N33/52 ,  G01N33/53

Abstract: A device and method for permitting the separation of plasma or serum from whole blood. The device comprises a matrix of hydrophilic sintered porous material to which at least one red blood cell agglutinating agent has been applied. According to a first method of using the device, a sample of whole blood is applied to a first end of the matrix and the red blood cells within the sample come in contact with the agglutinating agents present in the matrix. The blood cells agglutinate, and are entrapped in the interstices of the matrix, while substantially blood-cell-free serum or plasma accumulates near the outlet of the device. A filter means in liquid receiving relationship with the matrix functions to wick the serum or plasma from the matrix. According to an alternative aspect of the invention, a filter means in liquid receiving relationship with the outlet of the matrix functions to retain any blood cells which pass through the matrix as the filter means wicks the plasma or serum from the matrix. Additional agglutinating agents may be incorporated within the filter means to facilitate retention of blood cells which pass through the matrix. Another aspect of the present invention involves a device for the measurement of a serum or plasma sample, utilizing a matrix of sintered porous material, such as sintered glass, sintered steel, sintered ceramics, sintered plastics, and eguivalents thereof. The matrix is characterized by a reproducible fluid uptake capacity proportional to the fixed dimensions of said matrix, a minimal reactivity with plasma or serum components, and a hydrophilic internal surface which enables the matrix to collect and retain a predetermined volume of sample for analysis. A further aspect of the present invention provides a porous reagent delivery system comprising a matrix of sintered porous material containing at least one reagent in a freeze-dried state, and method for making same. The system allows the delivery of a unitized form of a freeze-dried reagent which is recoverable upon contact with a liquid.

公开(公告)号：CA2255909A1

公开(公告)日：1997-12-11

申请号：CA2255909

申请日：1997-06-03

Applicant: ABBOTT LAB

Inventor： SUBOTICH DANIEL T ,  MEES DAVID R ,  JOSEPH MARY K ,  POPE MARK R ,  PRY TERRY A ,  TARCHA PETER J ,  PUTMAN C BRENT

IPC: G01N33/543 ,  G01N33/547 ,  G01N33/545

Abstract: A method for increasing the binding activity of specific binding members bound to a solid phase material, e.g., a particle, that has been sterically stabilized. This increase in binding activity is brought about by degrading a steric stabilizer on the surface of the solid phase material. The method involves both immobilizing a specific binding member on the surface of a solid phase material and degrading a steric stabilizer on the surface of that solid phase material. In the preferred embodiment, the method involves the immobilization of a specific binding member on the surface of the sterically stabilized solid phase material, with subsequent degradation of the steric stabilizer.

公开(公告)号：DE69016813T2

公开(公告)日：1995-09-07

申请号：DE69016813

申请日：1990-04-06

Applicant: ABBOTT LAB

Inventor： AUNET DIANE L ,  ELMORE KRISTIN D ,  GEORGEVICH GRADIMIR G ,  OOSTA GARY M ,  SIEGEL NEAL A ,  JENG TZYY-WEN ,  PRY TERRY A ,  SILVERMAN CATHY R

IPC: G01N33/48 ,  B01D39/00 ,  B01D39/16 ,  B01D39/18 ,  B01D39/20 ,  B01L3/00 ,  C12M1/12 ,  C12M1/34 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q1/44 ,  C12Q1/48 ,  G01N33/49 ,  G01N33/52 ,  G01N33/53

Abstract: A device and method for permitting the separation of plasma or serum from whole blood. The device comprises a matrix of hydrophilic sintered porous material to which at least one red blood cell agglutinating agent has been applied. According to a first method of using the device, a sample of whole blood is applied to a first end of the matrix and the red blood cells within the sample come in contact with the agglutinating agents present in the matrix. The blood cells agglutinate, and are entrapped in the interstices of the matrix, while substantially blood-cell-free serum or plasma accumulates near the outlet of the device. A filter means in liquid receiving relationship with the matrix functions to wick the serum or plasma from the matrix. According to an alternative aspect of the invention, a filter means in liquid receiving relationship with the outlet of the matrix functions to retain any blood cells which pass through the matrix as the filter means wicks the plasma or serum from the matrix. Additional agglutinating agents may be incorporated within the filter means to facilitate retention of blood cells which pass through the matrix. Another aspect of the present invention involves a device for the measurement of a serum or plasma sample, utilizing a matrix of sintered porous material, such as sintered glass, sintered steel, sintered ceramics, sintered plastics, and eguivalents thereof. The matrix is characterized by a reproducible fluid uptake capacity proportional to the fixed dimensions of said matrix, a minimal reactivity with plasma or serum components, and a hydrophilic internal surface which enables the matrix to collect and retain a predetermined volume of sample for analysis. A further aspect of the present invention provides a porous reagent delivery system comprising a matrix of sintered porous material containing at least one reagent in a freeze-dried state, and method for making same. The system allows the delivery of a unitized form of a freeze-dried reagent which is recoverable upon contact with a liquid.

公开(公告)号：CA1326661C

公开(公告)日：1994-02-01

申请号：CA563413

申请日：1988-04-06

Applicant: ABBOTT LAB

Inventor： GRENIER FRANK C ,  PRY TERRY A ,  KOLACZKOWSKI LAWRENCE

IPC: C07J19/00 ,  G01N33/53 ,  G01N33/94

Abstract: A method for performing a diagnostic immunoassay by solid phase separation for digoxin. To a reaction mixture of a test sample and labeled anti-digoxin antibody, which forms a complex of any digoxin present in the test sample, is added a solid phase material having an immobilized ouabain triacetate derivative compound capable of binding any excess labeled antibody. The solid phase material is chosen to rapidly settle whereby a solid and liquid phase is formed. The liquid phase can then be extracted to measure the amount of digoxin-labeled antibody present therein. Ouabain triacetate derivative compounds possess sufficient affinity for anti-digoxin antibodies, and are therefore useful in a solid phase separation based digoxin immunoassay for settling out such antibodies without contributing to undesired background interference. These compounds are also less deleterious to assay performance because of low recognition when leached from the solid phase matrix thereby providing solid phase material with extended shelf life.