公开(公告)号：EP1403381A4

公开(公告)日：2005-01-12

申请号：EP02726495

申请日：2002-05-27

Applicant: FUSO PHARMACEUTICAL IND ,  OHNO TSUNEYA

Inventor： OHNO TSUNEYA ,  MATSUHISA AKIO

IPC: C12N15/31 ,  C12Q1/04 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/566 ,  G01N33/569

CPC classification number: C12Q1/689 ,  C12Q1/04

Abstract: A microorganism causative of an infection is quickly and highly sensitively detected and/or identified by obtaining phagocytes from a clinical specimen containing phagocytes originating in a living body, immobilizing the obtained phagocytes, treating the phagocytes so as to enhance the permeability of the phagocyte membrane and expose DNA of the microorganism causative of the infection which is anticipated as being contained in the phagocytes, and then using a detection DNA probe hybridizable with the DNA under stringent conditions.

公开(公告)号：EP0670373A4

公开(公告)日：1999-08-18

申请号：EP93923652

申请日：1993-10-25

Applicant: FUSO PHARMACEUTICAL IND ,  OHNO TSUNEYA

Inventor： OHNO TSUNEYA ,  HIROTSU TAKUO ,  KESHI HIROYUKI ,  MATSUHISA AKIO

IPC: C12N15/09 ,  C07H21/04 ,  C12Q1/04 ,  C12Q1/68 ,  C12R1/725

CPC classification number: C12Q1/6895

Abstract: The invention provides a probe useful for rapidly detecting and identifying fungi causative of infectious diseases by digesting completely a DNA extracted from a fungus Candidat albicans with EcoRI, inserting the same into an appropriate cloning vector, and picking up a probe containing DNA fragments unique to each fungus. The invention also provides a standard sequence suitable for making reference to a genomic DNA contained in a clinical specimen by elucidating the base sequence of the probe thus picked up.

公开(公告)号：EP1403369A4

公开(公告)日：2005-06-15

申请号：EP02778910

申请日：2002-05-27

Applicant: FUSO PHARMACEUTICAL IND

Inventor： OHNO TSUNEYA ,  MATSUHISA AKIO ,  KESHI HIROYUKI ,  ABE KANAKO ,  SUGIMOTO NORIHIKO ,  UEYAMA HIROSHI ,  EDA SOJI ,  UEHARA HIROTSUGU ,  IWAMI TAKAHISA ,  YAMAMOTO SEIJI ,  ARAKI HIROMASA

IPC: C12Q1/68 ,  G01N33/50 ,  C12N15/09 ,  C12N5/06 ,  C12N5/10 ,  G01N33/53

CPC classification number: G01N33/5047 ,  C12Q1/6841 ,  C12Q1/689 ,  G01N33/5005

Abstract: Phagocytes having taken a foreign microorganism which are prepared by bringing phagocytes into contact with the microorganism and then isolating the phagocytes; a process for producing the same; and a method and a kit with the use of the same. An experimental model whereby the phagocytosis function of phagocytes can be evaluated in vitro .

公开(公告)号：EP0652291A4

公开(公告)日：1999-08-18

申请号：EP93914968

申请日：1993-07-07

Applicant: FUSO PHARMACEUTICAL IND ,  OHNO TSUNEYA

Inventor： OHNO TSUNEYA ,  MATSUHISA AKIO ,  UEHARA HIROTSUGU ,  EDA SOJI

IPC: C12N15/09 ,  C12N15/11 ,  C12Q1/68 ,  C12R1/01 ,  C12R1/19 ,  C12R1/22 ,  C12R1/385 ,  G01N33/53 ,  G01N33/566

CPC classification number: C12Q1/689 ,  C12Q2600/156

Abstract: A probe useful for rapidly detecting and identifying bacteria causative of infectious diseases is provided by completely digesting DNAs extracted from principal bacteria causative of infectious diseases with Hind III, cloning the digestion product to a suitable vector, and selecting a probe containing DNA fragments peculiar to each of the bacteria. A standard DNA sequence suitable for comparison with that of a genomic DNA contained in a clinical specimen is provided by clarifying the base sequence of the selected probe.

公开(公告)号：DK1329519T3

公开(公告)日：2006-05-08

申请号：DK03075758

申请日：1993-07-07

Applicant: FUSO PHARMACEUTICAL IND ,  OHNO TSUNEYA

Inventor： MATSUHISA AKIO ,  UEHARA HIROTSUGU ,  OHNO TSUNEYA ,  EDA SOJI

IPC: C12N15/09 ,  C12N15/11 ,  C12Q1/68 ,  C12R1/01 ,  C12R1/19 ,  C12R1/22 ,  C12R1/385 ,  G01N33/53 ,  G01N33/566

Abstract: A probe for diagnosing infectious diseases in which the probe comprises the base sequence set out in SEQ. ID. No. 7 and specifically reacts with DNA of Staphylococcus epidermidis.

公开(公告)号：DE69333958D1

公开(公告)日：2006-02-02

申请号：DE69333958

申请日：1993-07-07

Applicant: FUSO PHARMACEUTICAL IND ,  OHNO TSUNEYA

Inventor： OHNO TSUNEYA ,  MATSUHISA AKIO ,  UEHARA HIROTSUGU ,  EDA SOJI

IPC: C12N15/09 ,  C12N15/11 ,  C12Q1/68 ,  C12R1/01 ,  C12R1/19 ,  C12R1/22 ,  C12R1/385 ,  G01N33/53 ,  G01N33/566

Abstract: A probe for diagnosing infectious diseases in which the probe comprises the base sequence set out in SEQ. ID. No. 7 and specifically reacts with DNA of Staphylococcus epidermidis.

公开(公告)号：AT314467T

公开(公告)日：2006-01-15

申请号：AT03075758

申请日：1993-07-07

Applicant: FUSO PHARMACEUTICAL IND ,  OHNO TSUNEYA

Inventor： OHNO TSUNEYA ,  MATSUHISA AKIO ,  UEHARA HIROTSUGU ,  EDA SOJI

IPC: C12N15/09 ,  C12N15/11 ,  C12Q1/68 ,  C12R1/01 ,  C12R1/19 ,  C12R1/22 ,  C12R1/385 ,  G01N33/53 ,  G01N33/566

Abstract: A probe for diagnosing infectious diseases in which the probe comprises the base sequence set out in SEQ. ID. No. 7 and specifically reacts with DNA of Staphylococcus epidermidis.

公开(公告)号：DE69333872D1

公开(公告)日：2005-10-20

申请号：DE69333872

申请日：1993-07-07

Applicant: FUSO PHARMACEUTICAL IND ,  OHNO TSUNEYA

Inventor： OHNO TSUNEYA ,  MATSUHISA AKIO ,  UEHARA HIROTSUGU ,  EDA SOJI

IPC: C12N15/09 ,  C12N15/11 ,  C12Q1/68 ,  C12R1/01 ,  C12R1/19 ,  C12R1/22 ,  C12R1/385 ,  G01N33/53 ,  G01N33/566

Abstract: A probe for diagnosing infectious diseases in which the probe comprises the base sequence set out in SEQ. ID. No. 7 and specifically reacts with DNA of Staphylococcus epidermidis.

公开(公告)号：DE69333008T2

公开(公告)日：2004-02-05

申请号：DE69333008

申请日：1993-07-07

Applicant: FUSO PHARMACEUTICAL IND ,  OHNO TSUNEYA

Inventor： OHNO TSUNEYA ,  MATSUHISA AKIO ,  UEHARA HIROTSUGU ,  EDA SOJI

IPC: C12N15/09 ,  C12N15/11 ,  C12Q1/68 ,  C12R1/01 ,  C12R1/19 ,  C12R1/22 ,  C12R1/385 ,  G01N33/53 ,  G01N33/566

Abstract: A probe for diagnosing infectious diseases in which the probe comprises the base sequence set out in SEQ. ID. No. 7 and specifically reacts with DNA of Staphylococcus epidermidis.

公开(公告)号：DE69334028T2

公开(公告)日：2007-05-10

申请号：DE69334028

申请日：1993-07-07

Applicant: FUSO PHARMACEUTICAL IND ,  OHNO TSUNEYA

Inventor： OHNO TSUNEYA ,  MATSUHISA AKIO ,  UEHARA HIROTSUGU ,  EDA SOJI

IPC: C12N15/09 ,  C12Q1/68 ,  C12N15/11 ,  C12R1/01 ,  C12R1/19 ,  C12R1/22 ,  C12R1/385 ,  G01N33/53 ,  G01N33/566

Abstract: A probe for diagnosing infectious diseases in which the probe comprises the base sequence set out in SEQ. ID. No. 7 and specifically reacts with DNA of Staphylococcus epidermidis.