中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

21 records (took 0.029 seconds)

    MICROORGANISMS AND METHODS FOR REDUCING BY-PRODUCTS
    3.
    发明申请
    MICROORGANISMS AND METHODS FOR REDUCING BY-PRODUCTS 审中-公开

    公开(公告)号：WO2021086944A1

    公开(公告)日：2021-05-06

    申请号：PCT/US2020/057707

    申请日：2020-10-28

    Applicant: GENOMATICA, INC.

    Inventor： NAGARAJAN, Harish ,  SCALCINATI, Gionata ,  YANG, Tae, Hoon

    IPC: C07C31/20 ,  C07C59/01 ,  C08G18/28 ,  A23L3/3463 ,  C12N15/52 ,  C12P7/18 ,  C12P7/26 ,  C12P7/42 ,  C12P7/62 ,  C12P7/6436 ,  C12Y102/01 ,  C12Y102/01004 ,  C12Y104/99001 ,  C12Y202/01006 ,  C12Y203/01169 ,  C12Y501/01001

    Abstract: The present disclosure provides microbial organisms having decreased production of unwanted by-products (e.g, pyruvate-, CO2-, TCA-derived by-products; acetate; ethanol; and/or, alanine) to enhance carbon flux through acetyl-CoA, which can increase production of acetyl- CoA derived compounds (e.g, 1,3-BDO, MMA, and (3R)-hydroxybutyl (3R)-hydroxybutyrate, or any other acetyl-CoA derived compounds), and products made from any of these compounds. Also provided are one or more exogenous nucleic acids encoding enzymes that can decrease production of unwanted by-products (e.g, aldehyde dehydrogenase, acetyl-CoA synthase, D-amino acid dehydrogenase, alanine racemase, and/or citrate synthase), and/or one or more gene attenuations occurring in genes (e.g., acetolactate synthase) that result in decreased production of unwanted by-products. Various combinations of the exogenous nucleic acids and gene deletions are also provided in the present disclosure. Methods of making and using the same, including methods for culturing cells, and for the production of the various products are also provided.

    ALCOHOL DEHYDROGENASE VARIANTS
    4.
    发明申请
    ALCOHOL DEHYDROGENASE VARIANTS 审中-公开
    酒精脱水变异

    公开(公告)号：WO2015051298A2

    公开(公告)日：2015-04-09

    申请号：PCT/US2014/059135

    申请日：2014-10-03

    Applicant: GENOMATICA, INC.

    Inventor： ANDRAE, Stefan ,  KUCHINSKAS, Michael Patrick ,  LI, Jingyi ,  NAGARAJAN, Harish ,  PHARKYA, Priti

    IPC: C12P7/16 ,  C12N1/21

    CPC classification number: C12N9/0006 ,  C12P7/18 ,  C12P7/24 ,  C12Y101/01001 ,  C12Y101/01244 ,  Y02P20/127

    Abstract: Described herein are non-natural NAD+-dependent alcohol dehydrogenases (ADHs) capable of at least two fold greater conversion of methanol or ethanol to formaldehyde or acetaldehyde, respectively, as compared to its unmodified counterpart. Nucleic acids encoding the non-natural alcohol dehydrogenases, as well as expression constructs including the nucleic acids, and engineered cells comprising the nucleic acids or expression constructs are described. Also described are engineered cells expressing a non-natural NAD + -dependent alcohol dehydrogenase, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.

    Abstract translation: 本文描述了与其未修饰的对应物相比，能够分别甲醇或乙醇转化为甲醛或乙醛的至少两倍的非天然NAD +依赖性醇脱氢酶（ADH）。 描述了编码非天然醇脱氢酶的核酸，以及包含核酸的表达构建体和包含核酸或表达构建体的工程化细胞。 还描述了表达非天然NAD +依赖性醇脱氢酶的工程细胞，任选地包括一种或多种另外的代谢途径转基因，甲醇代谢途径基因，靶产物途径基因，包括细胞的细胞培养组合物，促进生产的方法 的靶产物或其中间体，包括靶产物或中间体的组合物，以及由目标产物或中间体制成的产品。

    3-HYDROXYBUTYRYL-COA DEHYDROGENASE VARIANTS AND METHODS OF USE
    6.
    发明申请
    3-HYDROXYBUTYRYL-COA DEHYDROGENASE VARIANTS AND METHODS OF USE 审中-公开

    公开(公告)号：WO2018183640A1

    公开(公告)日：2018-10-04

    申请号：PCT/US2018/025086

    申请日：2018-03-29

    Applicant: GENOMATICA, INC.

    Inventor： HOFF, Kevin ,  TRACEWELL, Cara, Ann ,  CHAN, Kui ,  KUCHINSKAS, Michael ,  NAGARAJAN, Harish

    IPC: C12N9/04 ,  C12P7/42

    Abstract: The present disclosure provides thiolases and polypeptide variants of 3-hydroxybutyryl- CoA dehydrogenase, nucleic acids encoding the same, vectors comprising the nucleic acids, and cells comprising the polypeptide variants and/or thiolase, the nucleic acids, and/or the vectors. The present disclosure also provides methods of making and using the same, including methods for culturing cells, and for the production of various products, including 3-hydroxybutyryl-CoA (3-HB-CoA), 3-hydroxybutyraldehyde (3-HBal), 3-hydroxybutyrate (3-HB), 1,3-butanediol (1,3-BDO), and esters and amides thereof, and products made from any of these.

    VINYLISOMERASE-DEHYDRATASES, ALKENOL DEHYDRATASES, LINALOOL DEHYDRATASES AND/ CROTYL ALCOHOL DEHYDRATASES AND METHODS FOR MAKING AND USING THEM
    7.
    发明申请
    VINYLISOMERASE-DEHYDRATASES, ALKENOL DEHYDRATASES, LINALOOL DEHYDRATASES AND/ CROTYL ALCOHOL DEHYDRATASES AND METHODS FOR MAKING AND USING THEM 审中-公开
    乙烯衍生物脱乙酰壳多糖，烷基脱氢ES ES，，，，ES ES ES ES M M M M M M M M M M M M M M M M M M M M

    公开(公告)号：WO2016196233A1

    公开(公告)日：2016-12-08

    申请号：PCT/US2016/034488

    申请日：2016-05-26

    Applicant: GENOMATICA, INC. ,  BRASKEM S.A.

    Inventor： CULLER, Stephanie J. ,  HASELBECK, Robert J. ,  NAGARAJAN, Harish ,  GOUVEA, Iuri Estrada ,  KOCH, Daniel Johannes ,  LOPES, Mateus Schreiner Garcez ,  PARIZZI, Lucas Pederson

    IPC: C08F36/06 ,  C12N1/21 ,  C12N15/60 ,  C12N15/63 ,  C12N9/88

    CPC classification number: C12N9/88 ,  C07K2319/02 ,  C07K2319/034 ,  C12N9/90 ,  C12Y402/01 ,  C12Y402/01127 ,  C12Y504/04004

    Abstract: In alternative embodiments, provided are non-natural or genetically engineered vinylisomerase-dehydratase enzymes, including alkenol dehydratases, linalool dehydratases and crotyl alcohol dehydratases. In alternative embodiments, provided are non-natural or genetically engineered polypeptides having an activity comprising, for example, a vinylisomerase-dehydratase, an alkenol dehydratase, a linalool dehydratase and/or a crotyl alcohol dehydratase activity, or a combination thereof. In alternative embodiments, also provided are non-natural or genetically engineered nucleic acids (polynucleotides) encoding polypeptides described herein, expression or cloning vehicles comprising or having contained therein nucleic acids as described herein, and non-natural or genetically engineered cells comprising or having contained therein nucleic acids as described herein. In alternative embodiments, also provided are are methods for making various organic compounds, including methyl vinyl carbinol and butadiene.

    Abstract translation: 在替代实施方案中，提供非天然或遗传工程乙烯基异构酶 - 脱水酶，包括链烯醇脱水酶，里哪醇脱水酶和巴豆醇脱水酶。 在替代实施方案中，提供了具有活性的非天然或遗传工程多肽，其包括例如乙烯基异构酶脱水酶，链烯醇脱水酶，芳樟醇脱水酶和/或巴豆醇脱水酶活性，或其组合。 在替代实施方案中，还提供了编码本文所述的多肽的非天然或遗传工程化的核酸（多核苷酸），包含或含有本文所述的核酸的表达或克隆载体，以及非天然或遗传工程改造的细胞，其包含或含有 其中如本文所述的核酸。 在替代实施方案中，还提供了制备各种有机化合物，包括甲基乙烯基甲醇和丁二烯的方法。

    MICROORGANISMS AND METHODS FOR INCREASING CO-FACTORS
    8.
    发明申请
    MICROORGANISMS AND METHODS FOR INCREASING CO-FACTORS 审中-公开

    公开(公告)号：WO2021081185A1

    公开(公告)日：2021-04-29

    申请号：PCT/US2020/056831

    申请日：2020-10-22

    Applicant: GENOMATICA, INC.

    Inventor： NAGARAJAN, Harish ,  SCALCINATI, Gionata ,  YANG, Tae, Hoon

    IPC: C12N9/00 ,  C12N9/12 ,  C12N15/52

    Abstract: The present disclosure provides microbial organisms having increased availability of co-factors, such as NADPH, for increasing production of various products, including 1,3-BDO, MMA, (3R)-hydroxybutyl (3R)-hydroxybutyrate, amino acids, 3HB-CoA, adipate, caprolactam, 6-ACA, HMD A, or MAA, and products made from any of these. Also provided are one or more exogenous nucleic acids encoding an enzyme expressed in a sufficient amount to increase availability of NADPH, where the exogenous nucleic acid includes one or more of ATP-NADH kinase, pntAB, nadK, and gapN. Also provided are one or more gene attenuations occurring in genes, such as NDH-2, that result in an increased ratio of NADPH to NADH. Various combinations of the exogenous nucleic acids and gene deletions are also provided in the present disclosure. The present disclosure also provides methods of making and using the same, including methods for culturing cells, and for the production of the various products.

    PRENYLTRANSFERASE VARIANTS AND METHODS FOR PRODUCTION OF PRENYLATED AROMATIC COMPOUNDS
    10.
    发明申请
    PRENYLTRANSFERASE VARIANTS AND METHODS FOR PRODUCTION OF PRENYLATED AROMATIC COMPOUNDS 审中-公开

    公开(公告)号：WO2019173770A1

    公开(公告)日：2019-09-12

    申请号：PCT/US2019/021448

    申请日：2019-03-08

    Applicant: GENOMATICA, INC.

    Inventor： NOBLE, Michael A. ,  HOFF, Kevin G. ,  LECHNER, Anna ,  NAGARAJAN, Harish

    IPC: C12N9/10 ,  C12N15/82 ,  C12P7/22

    Abstract: Described herein are prenyltransferases including non-natural variants thereof having at least one amino acid substitution as compared to its corresponding natural or unmodified prenyltransferases and that are capable of at least two-fold greater rate of formation of cannabinoids such as cannabigerolic acid, cannabigerovarinic acid, cannabigerorcinic acid, and cannabigerol, as compared to a wild type control. Prenyltransferase variants also demonstrated regioselectivity to desired cannabinoid isomers such as CDBA (3-GOLA), 3-GDVA, 3-GOSA, and CBG (2-GOL). The prenyltransferase variants can be used to form prenylated aromatic compounds, and can be expressed in an engineered microbe having a pathway to such compounds, which include 3-GOLA, 3-GDVA, 3-GOSA, and CBG. 3-GOLA can be used for the preparation of cannabigerol (CBG), which can be used in therapeutic compositions.

Patent Agency Ranking