公开(公告)号：WO2022155554A1

公开(公告)日：2022-07-21

申请号：PCT/US2022/012644

申请日：2022-01-15

Applicant: GENOMATICA, INC.

Inventor： NAGARAJAN, Harish ,  YANG, Yae, Hoon ,  KHODAYARI, Ali ,  BACHAN, Shawn ,  GHATAK, Sankha ,  EAKLEY, Nicholas ,  SHAH, Amit ,  SHAH, Jinel ,  ZHANG, Bo

IPC: C12N1/21 ,  C12N15/52 ,  C12P7/18

Abstract: Disclosed are biosynthetic methods and engineered microorganisms that enhance or improve the biosynthesis of 6-aminocaproate, hexamethylenediamine, caproic acid, caprolactone, or caprolactam. The engineered microorganisms are modified to include, for example, upredulated and/or exogenous transporters for 6-aminocaproate, deletions and/or downregulated importers for 6-aminocaproate, upregulated and/or exogenous glutamate dehydrogenase, and/or deletions and/or downregulation of res A and/or cpsBG. Other engineered microorganisms may have disruptions of endogenous transporters for 6- aminocaproate.

公开(公告)号：WO2023076966A1

公开(公告)日：2023-05-04

申请号：PCT/US2022/078739

申请日：2022-10-26

Applicant: GENOMATICA, INC.

Inventor： SHAH, Amit, Mahendra ,  ZHANG, Deqiang ,  WARNER, Joseph, Roy ,  GRIFFIN, Benjamin, Matthew ,  SHAH, Jinel ,  COLQUITT, Justin, Robert

IPC: C12N9/02 ,  C12N15/63 ,  C12P7/24

Abstract: The invention provides an engineered carboxylic acid reductase (CAR) enzyme, a nucleic acid encoding the CAR enzyme, and a non-naturally occurring microbial organism comprising an exogenous nucleic acid encoding the CAR, an engineered transaminase (TA) enzyme, and/or a hexamethylenediamine (HMD) transaminase (TA2) enzyme. The invention provides a non-naturally occurring microbial organism that has a 1,6-hexanediol (HDO) pathway with a HDO pathway enzyme expressed in sufficient amounts to produce 6 aminocaproate semialdehyde, HDO, or both. The invention further provides a non-naturally occurring microbial organism that has an HMD pathway with a HMD pathway enzyme expressed in sufficient amounts to produce 6-aminocaproate semialdehyde, HMD, or both. The invention additionally provides bioderived HMD, 6-aminocaproate semialdehyde, and/or HDO and methods for producing bioderived HMD, 6-aminocaproate semialdehyde, and/or HDO.

公开(公告)号：EP4277976A1

公开(公告)日：2023-11-22

申请号：EP22740197.3

申请日：2022-01-15

Applicant: Genomatica, Inc.

Inventor： NAGARAJAN, Harish ,  YANG, Tae, Hoon ,  KHODAYARI, Ali ,  BACHAN, Shawn ,  GHATAK, Sankha ,  EAKLEY, Nicholas ,  SHAH, Amit ,  SHAH, Jinel ,  ZHANG, Bo

IPC: C12N1/21 ,  C12N15/52 ,  C12P7/18

公开(公告)号：EP4423259A1

公开(公告)日：2024-09-04

申请号：EP22888477.1

申请日：2022-10-26

Applicant: Genomatica, Inc.

Inventor： SHAH, Amit, Mahendra ,  ZHANG, Deqiang ,  WARNER, Joseph, Roy ,  GRIFFIN, Benjamin, Matthew ,  SHAH, Jinel ,  COLQUITT, Justin, Robert

IPC: C12N9/02 ,  C12N15/63 ,  C12P7/24

CPC classification number: C12N9/0008 ,  C12N9/1096 ,  C12Y206/01 ,  C12N15/52 ,  C12N15/70 ,  C12P7/18 ,  C12P13/001 ,  C12P13/005