公开(公告)号：WO2014066733A3

公开(公告)日：2014-06-19

申请号：PCT/US2013066765

申请日：2013-10-25

Applicant: LIFE TECHNOLOGIES CORP

Inventor： AGNEW BRIAN ,  AGGELER ROBERT ,  KANG HEE CHOL ,  CHEN AIMEI ,  GEE KYLE

IPC: G01N33/50

CPC classification number: A61K51/10 ,  C07K14/435 ,  C07K16/18 ,  C12P21/005 ,  C12P21/06 ,  G01N33/534 ,  G01N33/582 ,  G01N33/6854

Abstract: Provided herein are methods, compositions and kits for use in the site-specific labeling of glycoproteins comprising a combination of enzyme-mediated incorporation of modified sugars comprising a chemical handle and cycloaddition chemistry with a labeling molecule comprising a reactive group, a metal ion chelator, and/or a fluorophore.

Abstract translation: 本文提供了用于糖蛋白的位点特异性标记的方法，组合物和试剂盒，其包括酶介导的包含化学处理和环加成化学的修饰的糖的组合与包含反应性基团的标记分子，金属离子螯合剂， 和/或荧光团。

公开(公告)号：CA3107758A1

公开(公告)日：2020-02-13

申请号：CA3107758

申请日：2019-08-08

Applicant: LIFE TECHNOLOGIES CORP

Inventor： YANG JONGTAE ,  WANG XIN ,  HU YI-ZHEN ,  GEE KYLE ,  CHEN AIMEI ,  BENSON SCOTT ,  EVANS BRIAN ,  RUKAVISHNIKOV ALEKSEY

IPC: C09B69/00 ,  C09B11/14 ,  C12Q1/6869 ,  G01N33/58

Abstract: Silicon-substituted rhodamine compounds are disclosed herein. Also described herein are Si R dyes comprising at least one vinyl groupattached to the Si atom (10 position) of the SiR dye. Derivatives, functionalized versions, conjugates, kits, related synthetic methods and uses of Si R compounds also are provided. Silicon-rhodamine (SiR) dyes can provide bright fluorescence at far red wavelengths and exhibit good photostability. The compounds described herein can be useful for fluorescent labeling and detection of biological samples. The compounds have the general structure (I) below.

公开(公告)号：CL2021000340A1

公开(公告)日：2021-07-02

申请号：CL2021000340

申请日：2021-02-09

Applicant: LIFE TECHNOLOGIES CORP

Inventor： EVANS BRIAN ,  YANG JONGTAE ,  WANG XIN ,  HU YI-ZHEN ,  GEE KYLE ,  CHEN AIMEI ,  BENSON SCOTT ,  RUKAVISHNIKOV ALEKSEY

IPC: C09B11/14 ,  C09B69/00 ,  C12Q1/6869 ,  G01N33/58

Abstract: Los compuestos de rodamina sustituidos con silicio se describen en la presente. En la presente se describen también tintes de SiR que comprenden al menos un grupo vinilo unido al átomo de Si (posición 10) del tinte de SiR. También se proporcionan derivados, versiones funcionalizadas, conjugados, kits, métodos sintéticos relacionados y usos de compuestos de SiR. Los tintes de silicio-rodamina (SiR) pueden proporcionar una fluorescencia brillante en longitudes de onda de rojo lejano y mostrar buena fotoestabilidad. Los compuestos descritos en la presente pueden ser útiles para el marcado fluorescente y la detección de muestras biológicas.

公开(公告)号：SG11202101282QA

公开(公告)日：2021-03-30

申请号：SG11202101282Q

申请日：2019-08-08

Applicant: LIFE TECHNOLOGIES CORP

Inventor： YANG JONGTAE ,  WANG XIN ,  HU YI-ZHEN ,  GEE KYLE ,  CHEN AIMEI ,  BENSON SCOTT ,  EVANS BRIAN ,  RUKAVISHNIKOV ALEKSEY

IPC: C09B69/00 ,  C09B11/14 ,  C12Q1/6869 ,  G01N33/58

Abstract: Silicon-substituted rhodamine compounds are disclosed herein. Also described herein are SiR dyes comprising at least one vinyl group attached to the Si atom (10 position) of the SiR dye. Derivatives, functionalized versions, conjugates, kits, related synthetic methods and uses of SiR compounds also are provided. Silicon-rhodamine (SiR) dyes can provide bright fluorescence at far red wavelengths and exhibit good photostability. The compounds described herein can be useful for fluorescent labeling and detection of biological samples.

公开(公告)号：AU2019319901B2

公开(公告)日：2022-07-07

申请号：AU2019319901

申请日：2019-08-08

Applicant: LIFE TECHNOLOGIES CORP

Inventor： YANG JONGTAE ,  WANG XIN ,  HU YI-ZHEN ,  GEE KYLE ,  CHEN AIMEI ,  BENSON SCOTT ,  EVANS BRIAN ,  RUKAVISHNIKOV ALEKSEY

IPC: C09B69/00 ,  C09B11/14 ,  C12Q1/6869 ,  G01N33/58

Abstract: Silicon-substituted rhodamine compounds are disclosed herein. Also described herein are Si R dyes comprising at least one vinyl groupattached to the Si atom (10 position) of the SiR dye. Derivatives, functionalized versions, conjugates, kits, related synthetic methods and uses of Si R compounds also are provided. Silicon-rhodamine (SiR) dyes can provide bright fluorescence at far red wavelengths and exhibit good photostability. The compounds described herein can be useful for fluorescent labeling and detection of biological samples. The compounds have the general structure (I) below.

公开(公告)号：AU2019319901A1

公开(公告)日：2021-02-18

申请号：AU2019319901

申请日：2019-08-08

Applicant: LIFE TECHNOLOGIES CORP

Inventor： YANG JONGTAE ,  WANG XIN ,  HU YI-ZHEN ,  GEE KYLE ,  CHEN AIMEI ,  BENSON SCOTT ,  EVANS BRIAN ,  RUKAVISHNIKOV ALEKSEY

IPC: C09B69/00 ,  C09B11/14 ,  C12Q1/6869 ,  G01N33/58

Abstract: Silicon-substituted rhodamine compounds are disclosed herein. Also described herein are Si R dyes comprising at least one vinyl groupattached to the Si atom (10 position) of the SiR dye. Derivatives, functionalized versions, conjugates, kits, related synthetic methods and uses of Si R compounds also are provided. Silicon-rhodamine (SiR) dyes can provide bright fluorescence at far red wavelengths and exhibit good photostability. The compounds described herein can be useful for fluorescent labeling and detection of biological samples. The compounds have the general structure (I) below.