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公开(公告)号：WO2010075568A3
公开(公告)日：2010-10-28
申请号：PCT/US2009069502
申请日：2009-12-23
Applicant: LIFE TECHNOLOGIES CORP , LEHTO DENNIS
Inventor： LEHTO DENNIS
CPC classification number: G01N35/00029 , B01J2219/00286 , B01J2219/00585 , B01L3/502776 , B01L9/527 , B01L2200/027 , B01L2300/0816 , B01L2300/0877 , B01L2400/0427 , G01N2035/00148
Abstract: A device for performing biological sample reactions comprising a plurality of flow cells each with at least one port for receiving reaction fluids delivered to a chamber of each flow cell and a manifold configured to receive the plurality of flow cells, wherein the manifold is configured to receive at least one reaction fluid, and wherein each flow cell is configured with a sample holder wherein the sample holder contains biological sample.
Abstract translation: 一种用于执行生物样品反应的装置，该装置包括多个流动池，每个流动池具有至少一个用于接收输送到每个流动池的反应流体的端口和配置成接收多个流动池的歧管，其中该歧管配置成接收 至少一种反应流体，并且其中每个流动池都配置有样品架，其中样品架包含生物样品。
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2.发明申请QUANTITATIVE AMPLIFICATION AND DETECTION OF SMALL NUMBERS OF TARGET POLYNUCLEOTIDES 审中-公开定量放大和检测目标多核苷酸的小数字
公开(公告)号：WO2005066372A3
公开(公告)日：2006-01-19
申请号：PCT/US2004044052
申请日：2004-12-30
Applicant: APPLERA CORP , VANN CHARLES S , LAO KAIQIN , REED MARK , STERN SETH R , LEHTO DENNIS
Inventor： VANN CHARLES S , LAO KAIQIN , REED MARK , STERN SETH R , LEHTO DENNIS
CPC classification number: B01L7/52 , B01L3/502707 , B01L3/502715 , B01L2300/0861 , B01L2300/18 , B01L2400/06 , C12Q1/686 , C12Q2527/125
Abstract: Devices, assemblies, systems and methods described herein enable detection of as few as a single copy of a target nucleic acid molecule. Polynucleotides copied from a single or a small number of target nucleotide(s) within regions near to an initial copying site may be detected by optical or other methods as disclosed herein. Devices, assemblies and systems may comprise probes and/or primer molecules. Systems comprising optical assemblies, thermal assemblies and reaction assemblies (having reaction chambers for amplification of target nucleic acid molecules) are provided in which used reaction assemblies may be replaced to provide reusable devices. Systems comprising analytical assemblies and detection assemblies are provided in which an assay cartridge having assay chambers may engage a thermal assembly for amplification of target nucleic acid molecules. These devices, systems and methods offer the advantages of detection of as few as a single copy of a target nucleic acid molecule.
Abstract translation: 本文描述的装置，组件，系统和方法能够检测目标核酸分子的单拷贝数。 从初始复制部位附近的区域内单个或少数目标核苷酸复制的多核苷酸可以通过本文公开的光学或其它方法检测。 装置，组件和系统可以包括探针和/或引物分子。 提供了包括光学组件，热组件和反应组件（具有用于扩增靶核酸分子的反应室）的系统，其中可以更换所使用的反应组件以提供可重复使用的装置。 提供了包括分析组件和检测组件的系统，其中具有测定室的测定盒可以接合热组件以扩增靶核酸分子。 这些装置，系统和方法提供了检测目标核酸分子的单拷贝的优点。
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Patent Agency Ranking3.发明申请
公开(公告)号：WO2010117457A3
公开(公告)日：2011-03-24
申请号：PCT/US2010001053
申请日：2010-04-08
Applicant: APPLIED BIOSYSTEMS LLC , LIU DAVID , YANG JOON MO , SHARIATI MARYAM , WOO SAM , COX DAVID M , LEHTO DENNIS
Inventor： LIU DAVID , YANG JOON MO , SHARIATI MARYAM , WOO SAM , COX DAVID M , LEHTO DENNIS
CPC classification number: B01F3/0807 , B01F7/161 , B01F7/22 , B01F2215/0422 , B01F2215/0431 , B01F2215/0481
Abstract: A system and method are provided for large volume sample amplification adaptable for use with conventional PCR-based reactions as well as emulsion-based PCR reactions. A sample is retained in a pouch or flexible bag which permits bulk PCR amplification with efficient heat-transfer properties. For applications involving emulsion-based PCR amplification, the system and method provide improved uniformity in emulsion amplification and can be used to amplify large or small volume emulsions rapidly and reproducibly.
Abstract translation: 提供适用于常规基于PCR的反应以及基于乳液的PCR反应的大体积样品扩增的系统和方法。 将样品保留在袋或柔性袋中，其允许具有有效的热传递性质的大量PCR扩增。 对于涉及基于乳液的PCR扩增的应用，该系统和方法提供了乳液扩增的改进的均匀性，并可用于快速和可重复地扩增大体积或小体积的乳液。
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公开(公告)号：WO2007002588A3
公开(公告)日：2008-04-17
申请号：PCT/US2006024809
申请日：2006-06-23
Applicant: APPLERA CORP , SANDALL DONALD R , SOMACK RALPH , HARDING IAN A , LEHTO DENNIS
Inventor： SANDALL DONALD R , SOMACK RALPH , HARDING IAN A , LEHTO DENNIS
IPC: B01L3/02
CPC classification number: B01L3/502 , B01L3/0275 , B01L7/52 , B01L2200/0642 , B01L2300/0809 , B01L2300/0864 , B01L2400/0478 , B01L2400/0487 , B01L2400/0638 , B01L2400/0644 , G01N2035/103 , G01N2035/1032 , G01N2035/1034 , G01N2035/1062
Abstract: A pipette tip and system for aspiration of a biological sample, distribution to a plurality of sample chambers, and thermal cycling in the pipette tip.
Abstract translation: 用于抽吸生物样品的移液管尖端和系统，分配到多个样品室，以及移液管尖端中的热循环。
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公开(公告)号：WO2006116616A3
公开(公告)日：2007-09-27
申请号：PCT/US2006016060
申请日：2006-04-26
Applicant: APPLERA CORP , YUE MIN , LIU DAVID M , CHIANG YUH-MIN , YANG JOON MO , LEHTO DENNIS , VANN CHARLES S , BEARD NIGEL P , HARDING IAN A , VAN CAMP JOHN , DROMARETSKY ALEXANDER , ERMAKOV SERGEY V , OLDHAM MARK F , ROY JOY , SHARIATI MARYAM , ULMANELLA UMBERTO
Inventor： YUE MIN , LIU DAVID M , CHIANG YUH-MIN , YANG JOON MO , LEHTO DENNIS , VANN CHARLES S , BEARD NIGEL P , HARDING IAN A , VAN CAMP JOHN , DROMARETSKY ALEXANDER , ERMAKOV SERGEY V , OLDHAM MARK F , ROY JOY , SHARIATI MARYAM , ULMANELLA UMBERTO
IPC: C12M1/38
CPC classification number: C12Q1/6806 , B01J2219/00317 , B01J2219/00722 , B01L3/502707 , B01L3/502723 , B01L3/50853 , B01L2200/0684 , B01L2200/0689 , B01L2200/16 , B01L2300/041 , B01L2300/048 , B01L2300/0636 , B01L2300/0816 , B01L2300/0829 , B01L2300/0864 , B01L2300/087 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , B01L2400/0694 , G01N35/028 , Y10T436/2575
Abstract: Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials.
Abstract translation: 用于多个分析物检测的系统和方法包括用于分配包括衬底的生物样品的系统，其中所述衬底包括多个样品室，用于每个样品室的样品引入通道和用于每个样品室的排气通道。 该系统可以进一步包括包含在每个样品室中并被配置用于进入基底的生物样品的核酸分析的预加载试剂和被配置为与基底接触以密封每个样品室以便基本上防止 每个样品室中包含的样品从每个样品室流出。 底物可以由检测兼容和测定相容的材料构成。
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公开(公告)号：EP2057435A4
公开(公告)日：2011-04-20
申请号：EP07798040
申请日：2007-06-01
Applicant: LIFE TECHNOLOGIES CORP
Inventor： BOEGE STEVEN J , SHERIDAN JOHN ANDREW , ASLAM ALI P , LEHTO DENNIS , FOCARACCI JULIO , DROMARETSKY ALEXANDER , AU THOMAS C , OLDHAM MARK F , HENDERSON ERIC G , MIRCHANDANI VINOD , NORDMAN ERIC S , HABERSTROH MARC , SLUIS JOHANNES PAUL
CPC classification number: B01L7/52 , B01L2300/0636 , B01L2300/0829 , B01L2300/0877 , B01L2300/1822 , B01L2300/1844 , B01L2300/185
Abstract: A device for performing biological analysis may include at least one reaction chamber configured to receive at least one sample for biological analysis and a thermal system configured to modulate a temperature of the at least one reaction chamber to cycle a temperature of the at least one biological sample. The thermal system may include a cooling system configured to cool the at least one reaction chamber. The cooling system may include a cooling fluid source positioned distally from the at least one reaction chamber, the cooling fluid source being in flow communication with at least one conduit configured to flow cooling fluid from the cooling fluid source to at least one location in thermal communication with the at least one reaction chamber.
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7.发明公开SYSTEMS AND METHODS FOR MULTIPLE ANALYTE DETECTION 有权SYSTEME UND VERFAHREN ZUM NACHWEIS MEHRERER ANALYTE
公开(公告)号：EP1885839A4
公开(公告)日：2015-05-27
申请号：EP06751668
申请日：2006-04-26
Applicant: LIFE TECHNOLOGIES CORP
Inventor： YUE MIN , LIU DAVID M , CHIANG YUH-MIN , YANG JOON MO , LEHTO DENNIS , VANN CHARLES S , BEARD NIGEL P , HARDING IAN A , VAN CAMP JOHN , DROMARETSKY ALEXANDER , ERMAKOV SERGEY V , OLDHAM MARK F , ROY JOY , SHARIATI MARYAM , ULMANELLA UMBERTO
CPC classification number: C12Q1/6806 , B01J2219/00317 , B01J2219/00722 , B01L3/502707 , B01L3/502723 , B01L3/50853 , B01L2200/0684 , B01L2200/0689 , B01L2200/16 , B01L2300/041 , B01L2300/048 , B01L2300/0636 , B01L2300/0816 , B01L2300/0829 , B01L2300/0864 , B01L2300/087 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , B01L2400/0694 , G01N35/028 , Y10T436/2575
Abstract: Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials.
Abstract translation: 用于多个分析物检测的系统和方法包括用于分配包括衬底的生物样品的系统，其中所述衬底包括多个样品室，用于每个样品室的样品引入通道和用于每个样品室的排气通道。 该系统可以进一步包括包含在每个样品室中并被配置用于对进入基底的生物样品进行核酸分析的预加载试剂和被配置为与基底接触以密封每个样品室以便基本上防止 每个样品室中包含的样品不会从每个样品室流出。 底物可以由检测兼容和测定相容的材料构成。
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