公开(公告)号：WO2013009654A1

公开(公告)日：2013-01-17

申请号：PCT/US2012/045860

申请日：2012-07-06

Applicant: LIFE TECHNOLOGIES CORPORATION ,  BARBEE, Kristopher ,  CARPINO, Maximilian ,  WHEELING, Ray ,  BAJAK, Mik

Inventor： BARBEE, Kristopher ,  CARPINO, Maximilian ,  WHEELING, Ray ,  BAJAK, Mik

IPC: G01N35/00 ,  G01N35/10

CPC classification number: G01N35/0098 ,  G01N35/0099 ,  G01N35/10 ,  G01N35/1009 ,  Y10T436/255

Abstract: The present teachings provide apparatuses and methods for automated handling of samples, e.g., biological or chemical samples. The apparatuses and the methods of the present teachings allow automated performance of various sample manipulation steps without manual intervention. In a preferred embodiment, the present teachings provide apparatuses and methods for automated enrichment of templated beads produced by PCR.

Abstract translation: 本教导提供了用于自动处理样品（例如生物或化学样品）的设备和方法。 本教导的装置和方法允许自动执行各种样品操作步骤而无需手动干预。 在优选的实施方案中，本发明提供了通过PCR产生的模板化珠的自动富集的装置和方法。

公开(公告)号：WO2013055553A1

公开(公告)日：2013-04-18

申请号：PCT/US2012/058559

申请日：2012-10-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： BARBEE, Kristopher ,  DAVIDSON, John ,  HINZ, Wolfgang ,  LI, Shifeng ,  BUSTILLO, James

IPC: B01J19/00 ,  G01N27/414

CPC classification number: G01N27/4473 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00704 ,  B01J2219/00722 ,  C12Q1/6874 ,  G01N27/4145 ,  G01N27/4148 ,  G01N27/44743

Abstract: An apparatus includes a device substrate including an array of sensors. Each sensor of the array of sensors can include a electrode structure disposed at a surface of the device substrate. The apparatus further includes a wall structure overlying the surface of the device substrate and defining an array of wells at least partially corresponding with the array of sensors. The well structure including an electrode layer and an insulative layer.

Abstract translation: 一种装置包括包括传感器阵列的装置基板。 传感器阵列的每个传感器可以包括设置在器件基板的表面处的电极结构。 该装置还包括覆盖在装置基板的表面上的壁结构，并且限定至少部分对应于传感器阵列的一组阱阵列。 阱结构包括电极层和绝缘层。

公开(公告)号：WO2013165594A1

公开(公告)日：2013-11-07

申请号：PCT/US2013/031640

申请日：2013-03-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： BARBEE, Kristopher ,  JONES, Ryan ,  MCCUSKER, Sean ,  CARPINO, Maximilian ,  LEAMON, John ,  SCHULTZ, Jonathan ,  HOSHIZAKI, Jon

IPC: B01L7/00 ,  C12Q1/68 ,  G01N35/10 ,  G01N35/00

CPC classification number: C12Q1/6806 ,  B04B5/0421 ,  B04B13/00 ,  C12Q1/6869 ,  G01N35/00693 ,  G01N35/1011 ,  G01N2035/00495 ,  G01N2035/1013 ,  G01N2035/103

Abstract: An apparatus includes a robotic system providing movement in three orthogonal directions to an arm operable to receive a pipette tip and to facilitate movement of fluid into and out of the pipette tip. In addition, the apparatus can include a tray for receiving pipette tips, receptacles for receiving tubes, an apparatus for forming an emulsion, a centrifuge for breaking the emulsion, a device for forming particles that include copies of the polynucleotide, a device for enriching the particles and an apparatus for loading such particles onto a sensor array. The apparatus can further include receptacles for holding containers of reagent solutions. Optionally, the robot can include a gripper arm in addition to the pipette receiving arm. A method for calibrating the z-position of a translation device moving a pipette Hp is included. An imaging system is used for assessing attachement of pipette tip.

Abstract translation: 一种装置包括机器人系统，该机器人系统在三个正交方向上提供可操作以接收移液管尖端的臂并促进流体进入和移出移液管尖端的运动。 此外，该装置可以包括用于接收移液管吸头的托盘，用于接收管的容器，用于形成乳液的装置，用于破乳的离心机，用于形成包括多核苷酸的拷贝的颗粒的装置，用于富集 颗粒和用于将这种颗粒装载到传感器阵列上的装置。 该装置还可以包括容纳试剂溶液容器的容器。 可选地，除了移液管接收臂之外，机器人还可以包括夹持臂。 包括用于校准移动移液管Hp的翻译装置的z位置的方法。 成像系统用于评估移液管尖端的附着。

公开(公告)号：EP3068541B1

公开(公告)日：2020-10-28

申请号：EP14812319.3

申请日：2014-11-07

Applicant: Life Technologies Corporation

Inventor： BARBEE, Kristopher ,  ZHENG, Rui ,  MCCUSKER, Sean

IPC: B04B5/04 ,  B04B11/04 ,  G01N35/00 ,  G01N35/04

公开(公告)号：EP2844392A1

公开(公告)日：2015-03-11

申请号：EP13713643.8

申请日：2013-03-14

Applicant: Life Technologies Corporation

Inventor： BARBEE, Kristopher ,  JONES, Ryan ,  MCCUSKER, Sean ,  CARPINO, Maximilian ,  LEAMON, John ,  SCHULTZ, Jonathan ,  HOSHIZAKI, Jon

IPC: B01L7/00 ,  C12Q1/68 ,  G01N35/10 ,  G01N35/00

CPC classification number: C12Q1/6806 ,  B04B5/0421 ,  B04B13/00 ,  C12Q1/6869 ,  G01N35/00693 ,  G01N35/1011 ,  G01N2035/00495 ,  G01N2035/1013 ,  G01N2035/103

Abstract: An apparatus includes a robotic system providing movement in three orthogonal directions to an arm operable to receive a pipette tip and to facilitate movement of fluid into and out of the pipette tip. In addition, the apparatus can include a tray for receiving pipette tips, receptacles for receiving tubes, an apparatus for forming an emulsion, a centrifuge for breaking the emulsion, a device for forming particles that include copies of the polynucleotide, a device for enriching the particles and an apparatus for loading such particles onto a sensor array. The apparatus can further include receptacles for holding containers of reagent solutions. Optionally, the robot can include a gripper arm in addition to the pipette receiving arm. A method for calibrating the z-position of a translation device moving a pipette Hp is included. An imaging system is used for assessing attachement of pipette tip.