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    SEQUENCING DEVICE
    1.
    发明申请
    SEQUENCING DEVICE 审中-公开
    顺序设备

    公开(公告)号：WO2015195831A1

    公开(公告)日：2015-12-23

    申请号：PCT/US2015/036277

    申请日：2015-06-17

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： SCHULTZ, Jonathan ,  ROSWECH, Todd ,  HOSHIZAKI, Jon ,  CARRILLO, Albert ,  BALL, James

    IPC: B01L3/00 ,  G01N35/10

    CPC classification number: C12Q1/6869 ,  B01F1/0027 ,  B01F5/0683 ,  B01F11/0048 ,  B01F11/0071 ,  B01L3/502 ,  B01L3/505 ,  B01L3/527 ,  B01L3/563 ,  B01L2200/04 ,  B01L2200/16 ,  B01L2300/022 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0655 ,  B01L2400/0683 ,  C12Q1/6806 ,  G01N27/4145 ,  G01N35/1002 ,  G01N35/1095 ,  Y10T436/11 ,  Y10T436/143333

    Abstract: A method of preparing reagents includes inserting a cartridge into an instrument. The cartridge includes a plurality of reagent enclosures disposed in a cavity of the cartridge and exposing a port to an exterior of the cartridge. Each reagent enclosure includes a reagent container including a reagent and an internal cavity defining a compressible volume, an opening defined through the reagent container to the internal cavity. The method further includes connecting a plurality of fluid ports to the openings of the plurality of reagent enclosures; applying a solution through the fluid ports to at least partially fill the plurality of reagent enclosures; and cycling a pressure of the cavity, whereby for each of the reagent enclosures, during increasing pressure, the solution enters the internal cavity of the reagent container, combines with the reagent, and compresses the compressible volume, and during decreasing pressure, the compressible volume decreases and the reagent is ejected through the opening.

    Abstract translation: 制备试剂的方法包括将药筒插入仪器中。 盒包括设置在盒的空腔中并将端口暴露于盒的外部的多个试剂盒。 每个试剂盒包括试剂容器，其包括试剂和限定可压缩体积的内腔，通过试剂容器限定到内腔的开口。 该方法还包括将多个流体端口连接到多个试剂盒的开口; 通过所述流体端口施加溶液以至少部分地填充所述多个试剂外壳; 并且循环空腔的压力，由此对于每个试剂外壳，在增加的压力期间，溶液进入试剂容器的内腔，与试剂结合，并压缩可压缩体积，并且在减压期间，可压缩体积 减少，试剂通过开口排出。

    SYSTEM AND METHODS FOR MAKING AND PROCESSING EMULSIONS
    3.
    发明申请
    SYSTEM AND METHODS FOR MAKING AND PROCESSING EMULSIONS 审中-公开
    制备和处理乳液的系统和方法

    公开(公告)号：WO2012139125A2

    公开(公告)日：2012-10-11

    申请号：PCT/US2012/032786

    申请日：2012-04-09

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  SCHULTZ, Jonathan ,  NOBILE, John ,  REED, Brian ,  THWAR, Prasanna ,  ROSWECH, Todd

    Inventor： SCHULTZ, Jonathan ,  NOBILE, John ,  REED, Brian ,  THWAR, Prasanna ,  ROSWECH, Todd

    IPC: B01F3/08 ,  B01F5/04 ,  B01F13/00 ,  C12Q1/68 ,  B04B5/04 ,  B04B7/02 ,  B01L3/00 ,  B01L7/00

    CPC classification number: B01F5/0602 ,  B01F3/0807 ,  B01F5/0485 ,  B01F13/0059 ,  B01F2215/0037 ,  B01L3/502784 ,  B04B5/0414 ,  B04B7/02

    Abstract: An automated on-touch template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, an emulsion PCR (ePCR) thermocycling plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermocycling subsystem, in automated fashion. The ePCR subsystem can continuously thermocycle an inverse emulsion passed therethrough and includes static temperature zones and a consumable thermocycling plate. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.

    Abstract translation: 提供了一种自动化的在线模板珠粒制备系统，包括膜基乳液生成子系统，乳液PCR（ePCR）热循环板和子系统，以及连续离心乳液破乳和模板珠收集子系统。 乳液生成子系统在制备反相乳液时提供均匀性，并可用于快速且可再现地产生大体积或小体积的反相乳液。 提供一种乳液生成装置，其能够以自动方式向逆循环子系统提供连续的反相乳液流。 ePCR子系统可以连续热循环通过的反相乳液，并包括静态温度区和可消耗的热循环板。 连续离心子系统可以连续地破坏热循环的反相乳液并收集形成在反相乳液的微量微反应器液滴中的模板珠粒。

    MODULES AND CALIBRATION METHOD FOR A ROBOTIC POLYNUCLEOTIDE SAMPLE PREPARATION SYSTEM
    5.
    发明申请
    MODULES AND CALIBRATION METHOD FOR A ROBOTIC POLYNUCLEOTIDE SAMPLE PREPARATION SYSTEM 审中-公开
    机器人聚合物样品制备系统的模块和校准方法

    公开(公告)号：WO2013165594A1

    公开(公告)日：2013-11-07

    申请号：PCT/US2013/031640

    申请日：2013-03-14

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： BARBEE, Kristopher ,  JONES, Ryan ,  MCCUSKER, Sean ,  CARPINO, Maximilian ,  LEAMON, John ,  SCHULTZ, Jonathan ,  HOSHIZAKI, Jon

    IPC: B01L7/00 ,  C12Q1/68 ,  G01N35/10 ,  G01N35/00

    CPC classification number: C12Q1/6806 ,  B04B5/0421 ,  B04B13/00 ,  C12Q1/6869 ,  G01N35/00693 ,  G01N35/1011 ,  G01N2035/00495 ,  G01N2035/1013 ,  G01N2035/103

    Abstract: An apparatus includes a robotic system providing movement in three orthogonal directions to an arm operable to receive a pipette tip and to facilitate movement of fluid into and out of the pipette tip. In addition, the apparatus can include a tray for receiving pipette tips, receptacles for receiving tubes, an apparatus for forming an emulsion, a centrifuge for breaking the emulsion, a device for forming particles that include copies of the polynucleotide, a device for enriching the particles and an apparatus for loading such particles onto a sensor array. The apparatus can further include receptacles for holding containers of reagent solutions. Optionally, the robot can include a gripper arm in addition to the pipette receiving arm. A method for calibrating the z-position of a translation device moving a pipette Hp is included. An imaging system is used for assessing attachement of pipette tip.

    Abstract translation: 一种装置包括机器人系统，该机器人系统在三个正交方向上提供可操作以接收移液管尖端的臂并促进流体进入和移出移液管尖端的运动。 此外，该装置可以包括用于接收移液管吸头的托盘，用于接收管的容器，用于形成乳液的装置，用于破乳的离心机，用于形成包括多核苷酸的拷贝的颗粒的装置，用于富集 颗粒和用于将这种颗粒装载到传感器阵列上的装置。 该装置还可以包括容纳试剂溶液容器的容器。 可选地，除了移液管接收臂之外，机器人还可以包括夹持臂。 包括用于校准移动移液管Hp的翻译装置的z位置的方法。 成像系统用于评估移液管尖端的附着。

    MODELS FOR ANALYZING DATA FROM SEQUENCING-BY-SYNTHESIS OPERATIONS
    6.
    发明申请
    MODELS FOR ANALYZING DATA FROM SEQUENCING-BY-SYNTHESIS OPERATIONS 审中-公开
    用于分析合成数据的模型

    公开(公告)号：WO2012092455A2

    公开(公告)日：2012-07-05

    申请号：PCT/US2011/067776

    申请日：2011-12-29

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  REARICK, Todd ,  SCHULTZ, Jonathan

    Inventor： REARICK, Todd ,  SCHULTZ, Jonathan

    IPC: C12Q1/68 ,  G06F17/10 ,  C40B30/00

    CPC classification number: G06F19/24 ,  C12Q1/6869 ,  C12Q2527/119 ,  C12Q2563/159 ,  C12Q2565/607

    Abstract: Mathematical models for the analysis of signal data generated by sequencing of a polynucleotide strand using a pH-based method of detecting nucleotide incorporation(s). In an embodiment, the measured output signal from the reaction confinement region of a reactor array is mathematically modeled. The output signal may be modeled as a linear combination of one or more signal components, including a background signal component. This model is solved to determine the nucleotide incorporation signal. In another embodiment, the incorporation signal from the reaction confinement region of a reactor array is mathematically modeled.

    Abstract translation: 用于通过使用基于pH的检测核苷酸掺入的方法测序多核苷酸链产生的信号数据分析的数学模型。 在一个实施例中，来自反应堆阵列的反应限制区域的测量的输出信号在数学上被建模。 输出信号可以被建模为包括背景信号分量的一个或多个信号分量的线性组合。 该模型被解决以确定核苷酸掺入信号。 在另一个实施方案中，来自反应器阵列的反应限制区域的掺入信号在数学上被建模。

    PINCH FLOW REGULATOR
    7.
    发明申请
    PINCH FLOW REGULATOR 审中-公开
    PINCH流量调节器

    公开(公告)号：WO2015195692A1

    公开(公告)日：2015-12-23

    申请号：PCT/US2015/036071

    申请日：2015-06-16

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： SCHULTZ, Jonathan

    IPC: F16K7/07 ,  G05D16/06 ,  G05D16/18

    CPC classification number: F16K7/07 ,  B01L3/567 ,  B01L2200/06 ,  C12Q1/6869 ,  F16K7/04 ,  G05D16/0638 ,  G05D16/185

    Abstract: A valve for regulating fluid flow includes a housing base defining a lower cavity and comprising a pinch structure within the lower cavity, a gas inlet providing external access to the lower cavity, a base fluid inlet, and a base fluid outlet. A housing cover defines an upper cavity and comprises a cover fluid inlet and a cover fluid outlet. The cover fluid inlet is in fluidic communication with the base fluid outlet between the upper cavity and the lower cavity, and the cover fluid outlet provides external access from the upper cavity. A diaphragm is disposed between the housing base and the housing cover. A pinch plate is disposed in the lower cavity and comprises a pinch point disposed opposite the pinch structure. A pinch tube is in fluidic communication between the base fluid inlet and the base fluid outlet in the lower cavity.

    Abstract translation: 用于调节流体流动的阀包括限定下腔的壳体底座，并且在下腔体内包括夹紧结构，提供对下腔室的外部通路的气体入口，基础流体入口和基础流体出口。 壳体盖限定上腔，并且包括盖流体入口和盖流体出口。 覆盖流体入口与上腔和下腔之间的基础流体出口流体连通，并且盖流体出口提供从上腔体的外部通路。 隔膜设置在壳体基座和壳体盖之间。 压板设置在下腔体中，并且包括与夹紧结构相对设置的夹点。 夹管在底部流体入口和下腔体中的基础流体出口之间流体连通。

    PHASE-PROTECTING REAGENT FLOW ORDERINGS FOR USE IN SEQUENCING-BY-SYNTHESIS
    8.
    发明申请
    PHASE-PROTECTING REAGENT FLOW ORDERINGS FOR USE IN SEQUENCING-BY-SYNTHESIS 审中-公开
    用于按顺序合成使用的相保护试剂流动顺序

    公开(公告)号：WO2012138921A1

    公开(公告)日：2012-10-11

    申请号：PCT/US2012/032418

    申请日：2012-04-05

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  HUBBELL, Earl ,  SCHULTZ, Jonathan

    Inventor： HUBBELL, Earl ,  SCHULTZ, Jonathan

    IPC: C12Q1/68

    CPC classification number: C12Q1/6869 ,  C12Q1/6874 ,  C12Q2533/101 ,  C12Q2565/301 ,  C12Q2565/607

    Abstract: A method for nucleic acid sequencing includes disposing a plurality of template polynucleotide strands in a plurality of defined spaces disposed on a sensor array, at least some of the template polynucleotide strands having a sequencing primer and a polymerase operably bound therewith; exposing the template polynucleotide strands with the sequencing primer and a polymerase operably bound therewith to a series of flows of nucleotide species flowed according to a predetermined ordering; and determining, for each of the series of flows of nucleotide species, how many nucleotide incorporations occurred for that particular flow to determine a predicted sequence of nucleotides corresponding to the template polynucleotide strands, wherein the predetermined ordering (a) is not a series of consecutive repetitions of a 4-flow permutation of four different nucleotide species, (b) is not specifically tailored to a particular combination of a particular template polynucleotide strand to be sequenced and a particular sequencing primer to be used, and (c) comprises a phase-protecting flow ordering.

    Abstract translation: 用于核酸测序的方法包括将多个模板多核苷酸链设置在设置在传感器阵列上的多个限定空间中，至少一些模板多核苷酸链具有测序引物和与之可操作地结合的聚合酶; 用测序引物和可操作地与其结合的聚合物模板多核苷酸链暴露于根据预定排序流动的一系列核苷酸类型流; 并且对于核苷酸种类的每一系列流程，确定针对该特定流出现多少个核苷酸掺入以确定对应于模板多核苷酸链的预测的核苷酸序列，其中预定顺序（a）不是一系列连续的 重复四种不同核苷酸物种的4流置换，（b）不是特定的针对待测序的特定模板多核苷酸链和特定的待测序列的特定组合而定制的，（c） 保护流量顺序。

    ALTERNATIVE NUCLEOTIDE FLOWS IN SEQUENCING-BY-SYNTHESIS METHODS
    9.
    发明申请
    ALTERNATIVE NUCLEOTIDE FLOWS IN SEQUENCING-BY-SYNTHESIS METHODS 审中-公开
    序列合成方法中的替代核酸流

    公开(公告)号：WO2011156707A2

    公开(公告)日：2011-12-15

    申请号：PCT/US2011/039973

    申请日：2011-06-10

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  SCHULTZ, Jonathan ,  DAVIDSON, John F.

    Inventor： SCHULTZ, Jonathan ,  DAVIDSON, John F.

    IPC: C12Q1/68

    CPC classification number: C12Q1/6869 ,  B01L3/5027 ,  C12Q1/6874 ,  C12Q2537/155 ,  C12Q2563/149 ,  C12Q2565/301 ,  C12Q2565/629

    Abstract: A method for sequencing a polynucleotide strand by using sequencing-by-synthesis techniques. To address the problem of incomplete extension (IE) and/or carry forward (CF) errors that can occur in sequencing-by-synthesis reactions, an alternative flow ordering of dNTPs is used. In contrast to conventional flow orderings, the dNTPs are flowed in an ordering that is not a continuous repeat of an ordering of the four different dNTPs. This alternate flow ordering may reduce the loss of phasic synchrony in the population of template polynucleotide strands that result from IE and/or CF errors.

    Abstract translation: 通过使用按顺序合成技术测序多核苷酸链的方法。 为了解决在逐个合成反应中可能发生的不完全扩展（IE）和/或结转（CF）错误的问题，使用了dNTP的替代流顺序。 与常规流顺序相反，dNTP以不是四种不同dNTP的顺序的连续重复的顺序流动。 这种交替流顺序可以减少由IE和/或CF错误导致的模板多核苷酸链群体中的相位同步损失。

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