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    POLYMER-BASED EMULSION BREAKING METHODS
    2.
    发明申请
    POLYMER-BASED EMULSION BREAKING METHODS 审中-公开
    基于聚合物的乳液破乳方法

    公开(公告)号：WO2013165579A1

    公开(公告)日：2013-11-07

    申请号：PCT/US2013/031097

    申请日：2013-03-13

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： JONES, Ryan ,  CARPINO, Maximilian

    IPC: B01D17/04 ,  C07K1/14 ,  C10G33/04 ,  B01D21/00

    CPC classification number: B01D17/047 ,  B01D21/00 ,  B01D21/0009 ,  C07H21/00 ,  C10G33/04 ,  C12Q1/68

    Abstract: A method for breaking emulsions includes applying a polymer mixture to an emulsion. The emulsion can be energized, such as through centrifugation or vibration. In particular, the polymer mixture can be in liquid form. The polymer mixture includes first and second liquid polymer, the second liquid polymer being less hydrophilic than the first liquid polymer. In a water-in-oil emulsion, the less hydrophilic polymer can preferentially reside within the oil phase.

    Abstract translation: 用于破乳的方法包括将聚合物混合物施加到乳液上。 乳液可以被通电，例如通过离心或振动。 特别地，聚合物混合物可以是液体形式。 聚合物混合物包括第一和第二液体聚合物，第二液体聚合物的亲水性低于第一液体聚合物。 在油包水乳液中，亲水性较差的聚合物优先存在于油相中。

    MODULES AND CALIBRATION METHOD FOR A ROBOTIC POLYNUCLEOTIDE SAMPLE PREPARATION SYSTEM
    3.
    发明申请
    MODULES AND CALIBRATION METHOD FOR A ROBOTIC POLYNUCLEOTIDE SAMPLE PREPARATION SYSTEM 审中-公开
    机器人聚合物样品制备系统的模块和校准方法

    公开(公告)号：WO2013165594A1

    公开(公告)日：2013-11-07

    申请号：PCT/US2013/031640

    申请日：2013-03-14

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： BARBEE, Kristopher ,  JONES, Ryan ,  MCCUSKER, Sean ,  CARPINO, Maximilian ,  LEAMON, John ,  SCHULTZ, Jonathan ,  HOSHIZAKI, Jon

    IPC: B01L7/00 ,  C12Q1/68 ,  G01N35/10 ,  G01N35/00

    CPC classification number: C12Q1/6806 ,  B04B5/0421 ,  B04B13/00 ,  C12Q1/6869 ,  G01N35/00693 ,  G01N35/1011 ,  G01N2035/00495 ,  G01N2035/1013 ,  G01N2035/103

    Abstract: An apparatus includes a robotic system providing movement in three orthogonal directions to an arm operable to receive a pipette tip and to facilitate movement of fluid into and out of the pipette tip. In addition, the apparatus can include a tray for receiving pipette tips, receptacles for receiving tubes, an apparatus for forming an emulsion, a centrifuge for breaking the emulsion, a device for forming particles that include copies of the polynucleotide, a device for enriching the particles and an apparatus for loading such particles onto a sensor array. The apparatus can further include receptacles for holding containers of reagent solutions. Optionally, the robot can include a gripper arm in addition to the pipette receiving arm. A method for calibrating the z-position of a translation device moving a pipette Hp is included. An imaging system is used for assessing attachement of pipette tip.

    Abstract translation: 一种装置包括机器人系统，该机器人系统在三个正交方向上提供可操作以接收移液管尖端的臂并促进流体进入和移出移液管尖端的运动。 此外，该装置可以包括用于接收移液管吸头的托盘，用于接收管的容器，用于形成乳液的装置，用于破乳的离心机，用于形成包括多核苷酸的拷贝的颗粒的装置，用于富集 颗粒和用于将这种颗粒装载到传感器阵列上的装置。 该装置还可以包括容纳试剂溶液容器的容器。 可选地，除了移液管接收臂之外，机器人还可以包括夹持臂。 包括用于校准移动移液管Hp的翻译装置的z位置的方法。 成像系统用于评估移液管尖端的附着。

    PURIFICATION SYSTEMS AND METHODS
    4.
    发明申请
    PURIFICATION SYSTEMS AND METHODS 审中-公开
    净化系统和方法

    公开(公告)号：WO2012109615A1

    公开(公告)日：2012-08-16

    申请号：PCT/US2012/024756

    申请日：2012-02-10

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  HINZ, Wolfgang ,  JONES, Ryan ,  LIGHT, David

    Inventor： HINZ, Wolfgang ,  JONES, Ryan ,  LIGHT, David

    IPC: C08J3/12 ,  C12N15/10 ,  B01D15/34 ,  G01N15/02

    CPC classification number: C08J3/12 ,  B07B1/00 ,  C08J2300/14 ,  C12N15/1017

    Abstract: A method of preparing hydrogel particles includes applying a solution including a plurality of hydrogel particles to a stir cell. A retentate side of a filter defines a lower surface of the stir cell. The filter has the retentate side and a permeate side. The method further includes, while stirring the solution within the stir cell, dispensing a buffer solution at a first flow rate to the stir cell and drawing a permeate from the permeate side of the filter using a pump at a second flow rate, the permeate including a subset of the plurality of hydrogel particles.

    Abstract translation: 制备水凝胶颗粒的方法包括将包含多个水凝胶颗粒的溶液施加到搅拌槽。 过滤器的滞留侧限定了搅拌槽的下表面。 过滤器具有滞留侧和渗透侧。 该方法还包括在搅拌槽内搅拌溶液的同时，以第一流速向缓冲池分配缓冲溶液，并以第二流量使用泵从过滤器的渗透侧抽出渗透物，渗透物包括 多个水凝胶颗粒的一个子集。

    DEVICE PREPARATION USING CONDENSED NUCLEIC ACID PARTICLES
    5.
    发明公开
    DEVICE PREPARATION USING CONDENSED NUCLEIC ACID PARTICLES 审中-公开
    VORBEREITUNG EINER VORRICHTUNG UNTER VERWENDUNG VON KONDENSIERTENNUKLEINSÄUREPARTIKELN

    公开(公告)号：EP3044329A1

    公开(公告)日：2016-07-20

    申请号：EP14777230.5

    申请日：2014-09-12

    Applicant: Life Technologies Corporation

    Inventor： LUO, Guobin ,  SEDOVA, Marina ,  LIGHT, David ,  JONES, Ryan ,  ALANJARY, Mohammad

    IPC: C12Q1/68

    Abstract: A method of sequencing a nucleic acid strand includes receiving particles having nucleic acid strands coupled to a polymer matrix, exposing the particles to a solution including a condensing agent, and applying the particles to a surface, the particles depositing on the surface.

    Abstract translation: 对核酸链进行测序的方法包括接收具有与聚合物基质偶联的核酸链的颗粒，将颗粒暴露于包含缩合剂的溶液中，并将颗粒施加到表面上，颗粒沉积在表面上。

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