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公开(公告)号:WO2013138706A2
公开(公告)日:2013-09-19
申请号:PCT/US2013/032002
申请日:2013-03-15
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: PALLAS, Michael , NURSE, James C. , MAHER, Kevin , FONSECA, Jorge , GHENCIU, Eliodor , FOSTER, Evan
IPC: B01L3/00
CPC classification number: B01L3/50857 , B01L3/50 , B01L3/50851 , B01L2200/141 , B01L2300/0893 , B01L2300/0896 , B01L2300/161 , C12Q1/6806 , C12Q1/686 , G03F7/2002
Abstract: An article for holding a plurality of biological samples includes a substrate a substrate comprising a first surface and an opposing second surface and a plurality of reaction sites in the substrate. Each of the reaction sites extends from an opening in the first surface to an opening in the second surface. The reaction sites comprise a hexagonal shape and are configured to provide sufficient surface tension by capillary action to hold respective biological samples. The reaction sites have a density over at least a portion of the surfaces that is at least 170 holes per square millimeter. At least one of the surfaces may have a surface roughness characterized by an arithmetic average roughness (Ra) that is less than or equal to 5 nanometers.
Abstract translation: 用于保持多个生物样品的制品包括基底,该基底包括基底中的第一表面和相对的第二表面和多个反应位点。 每个反应位置从第一表面中的开口延伸到第二表面中的开口。 反应位点包括六边形形状,并且构造成通过毛细作用提供足够的表面张力以保持各自的生物样品。 反应位点的表面密度至少在每平方毫米170个孔以上。 至少一个表面可以具有由小于或等于5纳米的算术平均粗糙度(Ra)表征的表面粗糙度。
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公开(公告)号:WO2011041703A2
公开(公告)日:2011-04-07
申请号:PCT/US2010/051165
申请日:2010-10-01
Applicant: LIFE TECHNOLOGIES CORPORATION , BREVNOV, Maxim G. , NURSE, James C. , STRAY, James
Inventor: BREVNOV, Maxim G. , NURSE, James C. , STRAY, James
CPC classification number: G01N1/4005 , G01N2001/4016
Abstract: A device for sample processing can include at least one chamber having an egress, said chamber being configured to receive a sample for processing, a filter through which at least some sample portions in the at least one chamber flow, and a barrier member disposed in a first state to contain sample in the at least one chamber. Upon sufficient conditions, the barrier member can be alterable to a second state to permit flow of at least some sample portions contained in the chamber in a flow direction toward the egress and through the filter.
Abstract translation: 用于样品处理的装置可以包括具有出口的至少一个室,所述室被配置为接收用于处理的样品,过滤器,所述至少一个室中的至少一些样品部分通过所述过滤器和设置在所述室中的阻挡构件 第一状态以在至少一个室中容纳样品。 在足够的条件下,阻挡构件可以改变到第二状态,以允许包含在腔室中的至少一些样品部分沿流动方向流向出口并通过过滤器。
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公开(公告)号:EP2825310A2
公开(公告)日:2015-01-21
申请号:EP13713043.1
申请日:2013-03-15
Applicant: Life Technologies Corporation
Inventor: PALLAS, Michael , NURSE, James C. , MAHER, Kevin , FONSECA, Jorge , GHENCIU, Eliodor , FOSTER, Evan
CPC classification number: B01L3/50857 , B01L3/50 , B01L3/50851 , B01L2200/141 , B01L2300/0893 , B01L2300/0896 , B01L2300/161 , C12Q1/6806 , C12Q1/686 , G03F7/2002
Abstract: A system for performing biological reactions is provided. The system includes a chip including a substrate and a plurality of reaction sites. The plurality of reaction sites are each configured to include a liquid sample of at most one nanoliter. Further, the system includes a control system configured to initiate biological reactions within the liquid samples. The system further includes a detection system configured to detect biological reactions on the chip. According to various embodiments, the chip includes at least 20000 reaction sites. In other embodiments, the chip includes at least 30000 reaction sites.
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公开(公告)号:EP2483657A2
公开(公告)日:2012-08-08
申请号:EP10821349.7
申请日:2010-10-01
Applicant: Life Technologies Corporation
Inventor: BREVNOV, Maxim G. , NURSE, James C. , STRAY, James
CPC classification number: G01N1/4005 , G01N2001/4016
Abstract: A device for sample processing can include at least one chamber having an egress, said chamber being configured to receive a sample for processing, a filter through which at least some sample portions in the at least one chamber flow, and a barrier member disposed in a first state to contain sample in the at least one chamber. Upon sufficient conditions, the barrier member can be alterable to a second state to permit flow of at least some sample portions contained in the chamber in a flow direction toward the egress and through the filter.
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公开(公告)号:EP1789195B1
公开(公告)日:2010-10-27
申请号:EP05791667.8
申请日:2005-08-26
Applicant: Life Technologies Corporation
Inventor: VANN, Charles S. , BANERJEE, Debjyoti, Dept. of Mechanical Eng. , GEISER, Timothy G. , NURSE, James C. , BEARD, Nigel P.
IPC: B01L3/02
CPC classification number: G01N27/44743 , B01J4/008 , B01J2219/00315 , B01J2219/0036 , B01J2219/00418 , B01J2219/00653 , B01L3/0241 , B01L3/0268 , B01L3/502792 , B01L2300/0816 , B01L2300/0819 , B01L2300/089 , B01L2400/02 , B01L2400/0409 , B01L2400/0427
Abstract: A method for dispensing liquid for use in biological analysis may comprise positioning liquid to be dispensed via electrowetting. The positioning may comprise aligning the liquid with a plurality of predetermined locations. The method may further comprise dispensing the aligned liquid from the plurality of predetermined locations through a plurality of openings respectively aligned with the predetermined locations. The dispensing may be via electrowetting.
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