公开(公告)号：WO2019051237A1

公开(公告)日：2019-03-14

申请号：PCT/US2018/049966

申请日：2018-09-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： LIANG, Xiquan ,  POTTER, Robert ,  PENG, Lansha

IPC: C12N15/63

Abstract: The present disclosure relates to methods, kits, and compositions for improving the efficiency of homologous recombination. In particular, the disclosure relates to methods for cloning DNA molecules directly into a genome with the combined use of promoter trapping and short homology arms, nuclear localization signal, and/or binding one or more DNA binding agents (TAL effector domain or truncated guide RNA bound by Cas9) to specific sites thereby displacing or restructuring chromatin at the target locus, and/or it increasing the accessibility of the target locus to further enzymatic modifications. The methods and compositions provided herein are, inter alia, useful for genome editing and enhancing enzymatic processes involved therein.

公开(公告)号：WO2021071909A1

公开(公告)日：2021-04-15

申请号：PCT/US2020/054526

申请日：2020-10-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： LIANG, Xiquan ,  TANG, Pei-Zhong ,  POTTER, Robert

IPC: C12Q1/6806 ,  C12N15/10

Abstract: The present disclosure relates, in part, to improved methods of making single‑stranded DNA (ssDNA) from double‑stranded DNA (dsDNA), as well as use of the resulting ssDNA for genome engineering. The disclosure also relates, in part, to improved methods of genetic modification using single stranded DNA binding proteins.

公开(公告)号：WO2017205650A1

公开(公告)日：2017-11-30

申请号：PCT/US2017/034520

申请日：2017-05-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： LIANG, Xiquan ,  POTTER, Robert ,  RAVINDER, Namritha ,  KUMAR, Shantanu

IPC: C12N15/10 ,  C12N15/90

Abstract: The present disclosure generally relates to compositions and methods for improving the efficiency of homologous recombination. In particular, the disclosure relates to reagents and the use of such reagents.

Abstract translation: 本公开总体上涉及用于改善同源重组效率的组合物和方法。 具体而言，本公开涉及试剂和这些试剂的用途。

公开(公告)号：WO2017184799A1

公开(公告)日：2017-10-26

申请号：PCT/US2017/028482

申请日：2017-04-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： POTTER, Robert ,  CARTE, Jason

IPC: C12N15/11 ,  C12N15/10

Abstract: The present disclosure generally relates to compositions and methods for the genetic modification of cells. In particular, the disclosure relates to CRISPR reagents and the use of such reagents. Further disclosed are nucleic acid compositions with reduced cytotoxicity.

Abstract translation: 本公开总体上涉及用于细胞遗传修饰的组合物和方法。 特别地，本公开涉及CRISPR试剂和这些试剂的用途。 还公开了具有降低的细胞毒性的核酸组合物。

公开(公告)号：WO2016065364A1

公开(公告)日：2016-04-28

申请号：PCT/US2015/057401

申请日：2015-10-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： POTTER, Robert ,  CHESNUT, Jonathan ,  LIANG, Xiquan

IPC: C12N15/90 ,  C12N9/22

CPC classification number: C12N9/22 ,  C07K2319/00 ,  C07K2319/81 ,  C12N15/902 ,  C12N2310/20

Abstract: The present disclosure generally relates to compositions and methods for improving the efficiency of homologous recombination. In particular, the disclosure relates to reagents and the use of such reagents.

Abstract translation: 本公开一般涉及用于提高同源重组效率的组合物和方法。 特别地，本公开涉及试剂和这些试剂的用途。

公开(公告)号：WO2023039598A1

公开(公告)日：2023-03-16

申请号：PCT/US2022/076352

申请日：2022-09-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： CHESNUT, Jonathan ,  IHLAN, Alicia ,  POTTER, Robert ,  SONG, Youngzee

IPC: A61K38/46 ,  C12N9/22 ,  C12N15/52 ,  C12N15/62 ,  C12N15/90

Abstract: The present disclosure relates to recombinant nucleases, recombinant nucleases operatively linked to nucleic acid binding domains, and methods of making and using them.

公开(公告)号：WO2022006368A2

公开(公告)日：2022-01-06

申请号：PCT/US2021/040037

申请日：2021-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： KORE, Anilkumar ,  ANNAMALAI, Senthilvelan ,  MUTHIAN, Shanmugasundaram ,  POTTER, Robert ,  VONDERFECHT, Tyson

IPC: C07H21/00 ,  C07H1/00 ,  C12N15/00 ,  A61K31/7088 ,  A61K38/45 ,  A61K47/549 ,  C12N15/11 ,  C12N2310/3231 ,  C12N2310/3519 ,  C12N2320/32 ,  C12N9/1247 ,  C12Y207/07006

Abstract: This specification generally relates to trinucleotide RNA cap analogs, methods of use thereof, and kits comprising same. In particular, the trinucleotide cap analogs provided herein permit ready detection and/or isolation of capped RNA transcripts in vitro and translation of capped mRNAs in vivo.

公开(公告)号：WO2021178809A1

公开(公告)日：2021-09-10

申请号：PCT/US2021/021104

申请日：2021-03-05

Applicant: LIFE TECHNOLOGIES CORPORATION ,  THERMO FISHER SCIENTIFIC GENEART GMBH

Inventor： POTTER, Robert ,  NETUSCHIL, Nikolai

IPC: C12Q1/686 ,  C12N15/10 ,  C40B50/00 ,  C12N15/1031 ,  C40B50/06

Abstract: The present disclosure generally relates to compositions and methods for the synthesis of nucleic acid molecules with low error rates. Provided, as examples, are compositions and methods for high throughput synthesis and assembly of nucleic acid molecules, in many instances, with high sequence fidelity. In many instances, thermostable mismatch recognition proteins (e.g., thermostable mismatch binding protein, thermostable mismatch endonucleases) will be present in compositions and use methods provided.

公开(公告)号：WO2016057951A3

公开(公告)日：2016-04-14

申请号：PCT/US2015/054986

申请日：2015-10-09

Applicant: LIFE TECHNOLOGIES CORPORATION ,  THERMO FISHER SCIENTIFIC GENEART GMBH

Inventor： RAVINDER, Namritha ,  HEIL, Korbinian ,  GUO, Yizhu ,  LIANG, Xiquan ,  POTTER, Robert

IPC: C12Q1/68 ,  C12N15/113 ,  C12N15/10

Abstract: The present disclosure generally relates to compositions and methods for the genetic modification of cells. In particular, the disclosure relates to CRISPR reagents and the use of such reagents.

公开(公告)号：EP3464587A1

公开(公告)日：2019-04-10

申请号：EP17731961.3

申请日：2017-05-25

Applicant: Life Technologies Corporation

Inventor： LIANG, Xiquan ,  POTTER, Robert ,  RAVINDER, Namritha ,  KUMAR, Shantanu

IPC: C12N15/10 ,  C12N15/90