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    DETERMINING THE LIMIT OF DETECTION OF RARE TARGETS USING DIGITAL PCR
    1.
    发明申请
    DETERMINING THE LIMIT OF DETECTION OF RARE TARGETS USING DIGITAL PCR 审中-公开
    使用数字PCR测定稀有目标检测的限度

    公开(公告)号：WO2016197028A1

    公开(公告)日：2016-12-08

    申请号：PCT/US2016/035870

    申请日：2016-06-03

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： MAJUMDAR, Nivedita Sumi ,  WESSEL, Thomas

    IPC: G06F19/24 ,  G06F19/20

    CPC classification number: G06F19/24 ,  G06N7/005

    Abstract: A method for determining false positives calls in a biological data plot is provided. The method includes identifying a first data cluster as non-amplification data points within the biological data plot and identifying a second data cluster as wild-type positives within the biological data plot. The method further includes estimating a position in the biological data plot of a center of the first and second data clusters. The method further includes determining, for each data point within the first data cluster, a probability of belonging to the first data cluster and determining, for each data point within the second data cluster, a probability of belonging to the second data cluster. The method includes applying a probability threshold for each data point within the first and second data cluster to identify false positives.

    Abstract translation: 提供了一种用于在生物数据图中确定假阳性呼叫的方法。 该方法包括将第一数据簇识别为生物数据图中的非扩增数据点，并将第二数据簇识别为生物数据图中的野生型阳性。 该方法还包括估计第一和第二数据簇的中心的生物数据图中的位置。 该方法还包括为第一数据簇内的每个数据点确定属于第一数据簇的概率，并且针对第二数据簇内的每个数据点确定属于第二数据簇的概率。 该方法包括对第一和第二数据簇内的每个数据点应用概率阈值以识别误报。

    METHODS AND SYSTEMS FOR BACKGROUND SUBTRACTION IN AN IMAGE
    2.
    发明申请
    METHODS AND SYSTEMS FOR BACKGROUND SUBTRACTION IN AN IMAGE 审中-公开
    背景技术在图像中的方法和系统

    公开(公告)号：WO2013049440A1

    公开(公告)日：2013-04-04

    申请号：PCT/US2012/057710

    申请日：2012-09-28

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： CHENG, Francis T. ,  WESSEL, Thomas

    IPC: G06T7/00 ,  G06T5/50

    CPC classification number: G06T7/0012 ,  G06T5/50 ,  G06T7/70 ,  G06T2207/20068 ,  G06T2207/30024 ,  G06T2207/30072

    Abstract: A method for improving image quality is provided. The method includes receiving image data of a substrate, wherein the image data is generated by imaging the substrate, and an image is generated from the image data. The method further includes generating a background representation from a background noise portion of the image, wherein the background portion includes signal information undesired for further processing and generating a background subtracted image by subtracting the background representation from the image. In this way, a separate background image is not needed to subtract the background from the image including the regions-of-interest to improve image quality.

    Abstract translation: 提供了一种提高图像质量的方法。 该方法包括接收基板的图像数据，其中通过对基板进行成像而生成图像数据，并且从图像数据生成图像。 该方法还包括从图像的背景噪声部分生成背景表示，其中背景部分包括不期望用于进一步处理的信号信息，并且通过从图像中减去背景表示来生成背景相减图像。 以这种方式，不需要单独的背景图像从包括感兴趣区域的图像中减去背景以提高图像质量。

    METHODS AND SYSTEMS FOR BIOLOGICAL INSTRUMENT CALIBRATION
    3.
    发明申请
    METHODS AND SYSTEMS FOR BIOLOGICAL INSTRUMENT CALIBRATION 审中-公开
    生物仪器校准的方法和系统

    公开(公告)号：WO2016127124A2

    公开(公告)日：2016-08-11

    申请号：PCT/US2016/016882

    申请日：2016-02-05

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： CHU, Yong ,  MARKS, Jeffrey ,  FREUDENTHAL, Jacob ,  WESSEL, Thomas ,  WOO, David

    IPC: G01N21/27 ,  G01N21/64

    CPC classification number: G01N21/6428 ,  C12Q1/6851 ,  C12Q1/686 ,  G01N21/274 ,  G01N21/278 ,  G01N21/6452 ,  G01N21/6456 ,  G01N2021/6439 ,  G01N2021/6471 ,  G01N2201/127 ,  G01N2201/13 ,  G01N2333/922

    Abstract: In one exemplary embodiment, a method for calibrating an instrument is provided. The instrument includes an optical system capable of imaging florescence emission from a plurality of reaction sites. The method includes performing a region-of-interest (ROI) calibration to determine reaction site positions in an image. The method further includes performing a pure dye calibration to determine the contribution of a fluorescent dye used in each reaction site by comparing a raw spectrum of the fluorescent dye to a pure spectrum calibration data of the fluorescent dye. The method further includes performing an instrument normalization calibration to determine a filter normalization factor. The method includes performing an RNase P validation to validate the instrument is capable of distinguishing between two different quantities of sample.

    Abstract translation: 在一个示例性实施例中，提供了一种用于校准仪器的方法。 该仪器包括能够成像来自多个反应位点的荧光发射的光学系统。 该方法包括执行感兴趣区域（ROI）校准以确定图像中的反应位置位置。 该方法还包括进行纯染料校准，以通过将荧光染料的原始光谱与荧光染料的纯光谱校准数据进行比较来确定每个反应位点中使用的荧光染料的贡献。 该方法还包括执行仪器归一化校准以确定滤波器归一化因子。 该方法包括执行RNase P验证以验证仪器能够区分两种不同数量的样品。

    METHOD AND SYSTEM FOR DETERMINING AN AMPLIFICATION QUALITY METRIC
    5.
    发明申请
    METHOD AND SYSTEM FOR DETERMINING AN AMPLIFICATION QUALITY METRIC 审中-公开

    公开(公告)号：WO2013074204A3

    公开(公告)日：2013-05-23

    申请号：PCT/US2012/057707

    申请日：2012-09-28

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： WEISS, Howard ,  VAN HELEN, Eric ,  CHO, Jamie ,  WESSEL, Thomas

    IPC: G06F19/24

    Abstract: According to one exemplary embodiment, a method for providing a amplification quality metric to a user is provided. The method includes receiving amplification data from an amplification of a sample to generate an amplification curve. The amplification curve includes an exponential region and a transition region. The method further includes determining a first value of the transition region and determining a second value of the transition region. The first value is the beginning of the transition region and the second value is the end of the transition region. Next, the amplification quality metric is calculated based on at least the first value and the second value. Then, the amplification quality metric is displayed to the user.

    METHOD FOR STREAMLINING OPTICAL CALIBRATION
    6.
    发明申请
    METHOD FOR STREAMLINING OPTICAL CALIBRATION 审中-公开
    用于流光学校准的方法

    公开(公告)号：WO2013049776A1

    公开(公告)日：2013-04-04

    申请号：PCT/US2012/058197

    申请日：2012-09-30

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： FREUDENTHAL, Jacob K. ,  MARKS, Jeffrey A. ,  CHENG, Francis T. ,  WESSEL, Thomas

    IPC: G06T5/00 ,  G06T5/50

    CPC classification number: G06T7/0012 ,  G06T5/008 ,  G06T5/50 ,  G06T2207/10024 ,  G06T2207/10064 ,  G06T2207/10144 ,  G06T2207/20021 ,  G06T2207/20216 ,  G06T2207/30072

    Abstract: A method for calibrating a biological instrument is provided. The method comprises the steps of acquiring an image of at least one biological sample array, determining a first region of interest within the image, wherein the first region of interest comprises a first plurality of locations on the at least one biological array; and identifying within the first region of interest, a plurality of image elements associated with each of the first plurality of locations on the at least one biological array.

    Abstract translation: 提供了一种用于校准生物仪器的方法。 该方法包括以下步骤：获取至少一个生物样本阵列的图像，确定图像内的第一感兴趣区域，其中所述第一感兴趣区域包括所述至少一个生物阵列上的第一多个位置; 以及在所述第一感兴趣区域内识别与所述至少一个生物阵列上的所述第一多个位置中的每一个相关联的多个图像元素。

    METHODS AND SYSTEMS FOR INSTRUMENT VALIDATION
    7.
    发明申请
    METHODS AND SYSTEMS FOR INSTRUMENT VALIDATION 审中-公开
    仪器验证的方法和系统

    公开(公告)号：WO2016127032A1

    公开(公告)日：2016-08-11

    申请号：PCT/US2016/016730

    申请日：2016-02-05

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： WESSEL, Thomas ,  CHU, Yong ,  FREUDENTHAL, Jacob ,  WOO, David

    IPC: G01N21/27 ,  G01N21/64 ,  C12Q1/68 ,  G06F19/26

    CPC classification number: G01N21/6428 ,  G01N21/274 ,  G01N21/6452 ,  G01N21/6456 ,  G01N2021/6439 ,  G06F19/24

    Abstract: A method for validating an instrument is provided. The method includes receiving amplification data from a validation plate to generate a plurality of amplification curves (102, 202). The validation plate includes a sample of a first quantity and a second quantity, and each amplification curve includes an exponential region. The method further includes determining a set of fluorescence thresholds based on the exponential regions of the plurality of amplification curves (104, 204) and determining, for each fluorescence threshold of the set, a first set of cycle threshold (C t ) values of amplification curves generated from the samples of the first quantity and a second set of C t values of amplification curves generated from the samples of the second quantity (106, 206). The method includes calculating if the first and second quantities are sufficiently distinguishable based on C t values at each of the plurality of fluorescence thresholds (108, 208-218).

    Abstract translation: 提供了一种验证仪器的方法。 该方法包括从验证板接收放大数据以产生多个放大曲线（102,202）。 验证板包括第一数量和第二数量的样本，并且每个扩增曲线包括指数区域。 该方法还包括基于多个扩增曲线（104,204）的指数区域来确定一组荧光阈值，并且针对该组的每个荧光阈值确定放大曲线的第一组周期阈值（Ct）值 从第一数量的样本和从第二数量（106,206）的样本生成的第二组扩增曲线的Ctval值产生。 该方法包括基于多个荧光阈值（108,208-218）中的每一个的Ct值来计算第一和第二量是否足够可区分。

    METHODS AND SYSTEMS FOR A DIGITAL PCR EXPERIMENT DESIGNER
    9.
    发明申请
    METHODS AND SYSTEMS FOR A DIGITAL PCR EXPERIMENT DESIGNER 审中-公开
    数字PCR实验设计者的方法和系统

    公开(公告)号：WO2014043581A1

    公开(公告)日：2014-03-20

    申请号：PCT/US2013/059815

    申请日：2013-09-13

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： MAJUMDAR, Nivedita Sumi ,  WESSEL, Thomas ,  WOO, David C. ,  SIKORA, Marcin ,  JANAWAY, Gordon ,  RAIZADA, Shweta ,  LANKESTER, Joanna ,  MARKS, Jeffrey ,  WESSEL, Daniel

    IPC: G06F19/24

    CPC classification number: G06F19/20 ,  C12Q1/686 ,  G06F19/24

    Abstract: A computer-implemented method for designing a digital PCR (dPCR) experiment is provided. The method includes receiving, from a user, a selection of optimization type. The optimization type may be maximizing the dynamic range, minimizing the number of substrates including reaction sites needed for the experiment, determining a dilution factor, or determining the lower limit of detection, for example. The method further includes receiving, from the user, a precision measure for an experiment, and a minimum concentration of a target in a reaction site for the experiment. The method also includes determining a set of dPCR experiment design factors for the experiment based on the optimization type. The set of dPCR experiment design factors is then displayed to the user.

    Abstract translation: 提供了一种用于设计数字PCR（dPCR）实验的计算机实现方法。 该方法包括从用户接收优化类型的选择。 优化类型可以使动态范围最大化，例如最小化包括实验所需的反应位点，确定稀释因子或确定检测下限的底物数量。 该方法还包括从用户接收实验的精确度量，以及用于实验的反应部位中靶的最小浓度。 该方法还包括基于优化类型确定实验的一组dPCR实验设计因子。 然后将该组dPCR实验设计因子显示给用户。

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