公开(公告)号：AU2006330947A1

公开(公告)日：2007-07-05

申请号：AU2006330947

申请日：2006-12-21

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： BIBILLO AREK ,  MITSIS PAUL ,  PHAM THANG ,  CHRISTIANS FRED C ,  MURPHY DEVON ,  PARK INSIL ,  HANZEL DAVID K ,  LYLE JOHN ,  RANK DAVID R ,  CLARK SONYA ,  PELUSO PAUL ,  OTTO GEOFF

IPC: C12Q1/68

Abstract: Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.

公开(公告)号：AU2006330947B2

公开(公告)日：2012-04-12

申请号：AU2006330947

申请日：2006-12-21

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： BIBILLO AREK ,  MITSIS PAUL ,  PHAM THANG ,  CHRISTIANS FRED C ,  MURPHY DEVON ,  PARK INSIL ,  HANZEL DAVID K ,  LYLE JOHN ,  RANK DAVID R ,  CLARK SONYA ,  PELUSO PAUL ,  OTTO GEOFF

IPC: C12Q1/68

Abstract: Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.

公开(公告)号：ES2648313T3

公开(公告)日：2017-12-29

申请号：ES16157104

申请日：2006-12-21

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： HANZEL DAVID ,  OTTO GEOFF ,  MURPHY DEVON ,  PELUSO PAUL ,  PHAM THANG ,  RANK DAVID ,  MITSIS PAUL ,  CHRISTIANS FRED ,  BIBILLO AREK ,  PARK INSIL ,  CLARK SONYA ,  LYLE JOHN

IPC: C12Q1/68

Abstract: Una composición que comprende: una ADN polimerasa recombinante, comprendiendo dicha polimerasa recombinante una secuencia de aminoácidos que es al menos un 70 % idéntica a la SEQ ID NO:1 y comprende una sustitución de aminoácido en un sitio correspondiente a K512 respecto a la SEQ ID NO:1; y un análogo nucleotídico que está marcado en un grupo fosfato, en el que el resto de base del análogo se selecciona del grupo que consiste en adenina, timina, guanina y citidina.

公开(公告)号：CA2633524A1

公开(公告)日：2007-07-05

申请号：CA2633524

申请日：2006-12-21

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： BIBILLO AREK ,  CHRISTIANS FRED C ,  PARK INSIL ,  LYLE JOHN ,  OTTO GEOFF ,  PHAM THANG ,  RANK DAVID R ,  MURPHY DEVON ,  CLARK SONYA ,  MITSIS PAUL ,  PELUSO PAUL ,  HANZEL DAVID K

IPC: C12Q1/68

Abstract: Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.

公开(公告)号：WO2007076057A3

公开(公告)日：2008-06-26

申请号：PCT/US2006049122

申请日：2006-12-21

Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  HANZEL DAVID K ,  OTTO GEOFF ,  MURPHY DEVON ,  PELUSO PAUL ,  PHAM THANG ,  RANK DAVID R ,  MITSIS PAUL ,  CHRISTIANS FRED C ,  BIBILLO AREK ,  PARK INSIL ,  CLARK SONYA ,  LYLE JOHN

Inventor： HANZEL DAVID K ,  OTTO GEOFF ,  MURPHY DEVON ,  PELUSO PAUL ,  PHAM THANG ,  RANK DAVID R ,  MITSIS PAUL ,  CHRISTIANS FRED C ,  BIBILLO AREK ,  PARK INSIL ,  CLARK SONYA ,  LYLE JOHN

IPC: C12Q1/68

CPC classification number: C12N9/1252 ,  C07K2319/20 ,  C12P19/34 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Y207/07007 ,  Y02P20/52

Abstract: Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.

Abstract translation: 提供了包含具有改善核苷酸类似物进入活性位点区域并与活性位点区域中的核苷酸类似物配位的特征的聚合酶的组合物。 还提供了制备聚合酶和在测序和DNA复制和扩增中使用聚合酶的方法以及聚合酶活性的动力学模型和使用该模型的计算机实现的方法。

公开(公告)号：EP1963536A4

公开(公告)日：2010-03-03

申请号：EP06848948

申请日：2006-12-21

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： HANZEL DAVID K ,  OTTO GEOFF ,  MURPHY DEVON ,  PELUSO PAUL ,  PHAM THANG ,  RANK DAVID R ,  MITSIS PAUL ,  CHRISTIANS FRED C ,  BIBILLO AREK ,  PARK INSIL ,  CLARK SONYA ,  LYLE JOHN

IPC: C12Q1/68

CPC classification number: C12N9/1252 ,  C07K2319/20 ,  C12P19/34 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Y207/07007 ,  Y02P20/52

Abstract: Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.