公开(公告)号：US20220389517A1

公开(公告)日：2022-12-08

申请号：US17815082

申请日：2022-07-26

Applicant: SHIZUOKA PREFECTURE ,  SHIZUOKA PREFECTURAL UNIVERSITY CORPORATION

Inventor： Ken YAMAGUCHI ,  Masatoshi KUSUHARA ,  Masakuni SERIZAWA ,  Tohru MOCHIZUKI ,  Keiichi OHSHIMA ,  Keiichi HATAKEYAMA ,  Kenichi URAKAMI ,  Shumpei OHNAMI ,  Yasuto AKIYAMA ,  Kouji MARUYAMA ,  Kengo INOUE ,  Yuji SHIMODA ,  Takeshi NAGASHIMA ,  Yoshinobu ISHIKAWA

IPC: C12Q1/6886 ,  G01N33/574

Abstract: An object of the present invention is to provide a method for predicting a risk of developing cancer. DNA samples were prepared from blood and cancer tissues of 2480 cancer patients and analyzed for the nucleotide sequences of exon regions using NGS. As a result, among the cancer patients, 7 patients were confirmed to have D49H mutation or A159D mutation which is a germ cell mutation.

公开(公告)号：US20240376549A1

公开(公告)日：2024-11-14

申请号：US18685363

申请日：2022-08-23

Applicant: SHIZUOKA PREFECTURE ,  SRL & SHIZUOKA CANCER CENTER COLLABORATIVE LABORATORIES INC.

Inventor： Keiichi HATAKEYAMA ,  Takashi SUGINO ,  Koji MURAMATSU ,  Keiichi OHSHIMA ,  Hirotsugu KENMOTSU ,  Kenichi URAKAMI ,  Yuji SHIMODA ,  Takeshi NAGASHIMA ,  Yuichi KAWANISHI ,  Ryutaro FUKUMURA

IPC: C12Q1/6886 ,  C12Q1/6806 ,  C12Q1/6874

Abstract: Provided are a method for detecting a gene mutation using an FFPE tissue section containing tumor cells regardless of the percentage of tumor cells, the method being capable of increasing the number of detectable gene mutations and mutant allele frequency, and a method capable of differentiating, even in the absence of blood samples, a somatic cell mutation from a germ cell line mutation. A method for detecting a gene mutation according to the present invention comprises: a dissociation step for dissociating a single cell population from a formalin-fixed paraffin-embedded tissue section containing tumor cells; a separation step for obtaining a tumor fraction containing the tumor cells from the single cell population; a collection step for collecting a nucleic acid molecule from the tumor fraction; and a sequencing step for subjecting the nucleic acid molecule to sequencing.

公开(公告)号：US20180080935A1

公开(公告)日：2018-03-22

申请号：US15656896

申请日：2017-07-21

Applicant: SHIZUOKA PREFECTURE

Inventor： Tohru MOCHIZUKI ,  Keiichi OHSHIMA ,  Keiichi HATAKEYAMA ,  Ken YAMAGUCHI ,  Kanako NAKAO

IPC: G01N33/574

CPC classification number: G01N33/57438 ,  G01N2333/70596 ,  G01N2800/7028

Abstract: An object of the present invention is to provide a method for collecting highly accurate data for diagnosis, useful in diagnosing the presence or absence of extrahepatic bile duct carcinoma, intrahepatic bile duct carcinoma, or gallbladder carcinoma, and a kit for diagnosis.When the concentration of CEACAM1 in a blood sample collected from a test subject is detected using a kit for diagnosis comprising an antibody specifically binding to CEACAM1 or a labeled product thereof, or the like and the CEACAM1 concentration is higher than the concentration of CEACAM1 in a blood sample derived from a non-carcinoma control subject or higher than a certain threshold value (cutoff value), data can be collected for diagnosing the test subject as having a high possibility of having extrahepatic bile duct carcinoma, intrahepatic bile duct carcinoma, or gallbladder carcinoma.

公开(公告)号：US20160153872A1

公开(公告)日：2016-06-02

申请号：US14978115

申请日：2015-12-22

Applicant: SHIMADZU CORPORATION ,  SHIZUOKA PREFECTURE

Inventor： Yutaka AOKI ,  Yukari UMINO ,  Taka-Aki SATO ,  Isamu HAYASHI ,  Keiichi HATAKEYAMA ,  Shun-ichiro OGURA

IPC: G01N1/30 ,  G01N33/68

CPC classification number: G01N1/30 ,  G01N33/68 ,  G01N33/6848

Abstract: A protein retrieval treatment system used for activating proteins contained in a deparaffinized tissue section obtained by removing paraffin from a formalin-fixed paraffinembedded tissue section. The protein retrieval treatment system includes: a dispensing unit for dispensing a retrieval treatment solution over a dispensing area including a measurement area on the deparaffinized tissue section; and a moist-heat treatment unit for heating, in a saturated water vapor, the deparaffinized tissue section with the dispensing area covered with the retrieval treatment solution.

Abstract translation: 一种蛋白质回收处理系统，用于激活通过从福尔马林固定的石蜡包裹的组织切片中除去石蜡而获得的脱石蜡组织切片中所含的蛋白质。 蛋白质回收处理系统包括：分配单元，用于在包括脱蜡组织部分上的测量区域的分配区域上分配回收处理溶液; 以及湿热处理单元，用于在饱和水蒸汽中加热脱蜡组织部分，其中所述分配区域被所述回收处理溶液覆盖。

公开(公告)号：US20200255901A1

公开(公告)日：2020-08-13

申请号：US16322895

申请日：2017-08-03

Applicant: Shizuoka Prefecture ,  Shizuoka Prefectural University Corporation

Inventor： Ken YAMAGUCHI ,  Masatoshi KUSUHARA ,  Masakuni SERIZAWA ,  Tohru MOCHIZUKI ,  Keiichi OHSHIMA ,  Keiichi HATAKEYAMA ,  Kenichi URAKAMI ,  Shumpei OHNAMI ,  Yasuto AKIYAMA ,  Kouji MARUYAMA ,  Kengo INOUE ,  Yuji SHIMODA ,  Takeshi NAGASHIMA ,  Yoshinobu ISHIKAWA

IPC: C12Q1/6886 ,  G01N33/574

Abstract: An object of the present invention is to provide a method for predicting a risk of developing cancer. DNA samples were prepared from blood and cancer tissues of 2480 cancer patients and analyzed for the nucleotide sequences of exon regions using NGS. As a result, among the cancer patients, 7 patients were confirmed to have D49H mutation or A159D mutation which is a germ cell mutation.