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公开(公告)号：US20220389517A1
公开(公告)日：2022-12-08
申请号：US17815082
申请日：2022-07-26
Inventor： Ken YAMAGUCHI , Masatoshi KUSUHARA , Masakuni SERIZAWA , Tohru MOCHIZUKI , Keiichi OHSHIMA , Keiichi HATAKEYAMA , Kenichi URAKAMI , Shumpei OHNAMI , Yasuto AKIYAMA , Kouji MARUYAMA , Kengo INOUE , Yuji SHIMODA , Takeshi NAGASHIMA , Yoshinobu ISHIKAWA
IPC: C12Q1/6886 , G01N33/574
Abstract: An object of the present invention is to provide a method for predicting a risk of developing cancer. DNA samples were prepared from blood and cancer tissues of 2480 cancer patients and analyzed for the nucleotide sequences of exon regions using NGS. As a result, among the cancer patients, 7 patients were confirmed to have D49H mutation or A159D mutation which is a germ cell mutation.
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公开(公告)号：US20240376549A1
公开(公告)日：2024-11-14
申请号：US18685363
申请日：2022-08-23
Inventor： Keiichi HATAKEYAMA , Takashi SUGINO , Koji MURAMATSU , Keiichi OHSHIMA , Hirotsugu KENMOTSU , Kenichi URAKAMI , Yuji SHIMODA , Takeshi NAGASHIMA , Yuichi KAWANISHI , Ryutaro FUKUMURA
IPC: C12Q1/6886 , C12Q1/6806 , C12Q1/6874
Abstract: Provided are a method for detecting a gene mutation using an FFPE tissue section containing tumor cells regardless of the percentage of tumor cells, the method being capable of increasing the number of detectable gene mutations and mutant allele frequency, and a method capable of differentiating, even in the absence of blood samples, a somatic cell mutation from a germ cell line mutation. A method for detecting a gene mutation according to the present invention comprises: a dissociation step for dissociating a single cell population from a formalin-fixed paraffin-embedded tissue section containing tumor cells; a separation step for obtaining a tumor fraction containing the tumor cells from the single cell population; a collection step for collecting a nucleic acid molecule from the tumor fraction; and a sequencing step for subjecting the nucleic acid molecule to sequencing.
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3.发明申请公开(公告)号：US20180080935A1
公开(公告)日：2018-03-22
申请号：US15656896
申请日：2017-07-21
Applicant: SHIZUOKA PREFECTURE
Inventor： Tohru MOCHIZUKI , Keiichi OHSHIMA , Keiichi HATAKEYAMA , Ken YAMAGUCHI , Kanako NAKAO
IPC: G01N33/574
CPC classification number: G01N33/57438 , G01N2333/70596 , G01N2800/7028
Abstract: An object of the present invention is to provide a method for collecting highly accurate data for diagnosis, useful in diagnosing the presence or absence of extrahepatic bile duct carcinoma, intrahepatic bile duct carcinoma, or gallbladder carcinoma, and a kit for diagnosis.When the concentration of CEACAM1 in a blood sample collected from a test subject is detected using a kit for diagnosis comprising an antibody specifically binding to CEACAM1 or a labeled product thereof, or the like and the CEACAM1 concentration is higher than the concentration of CEACAM1 in a blood sample derived from a non-carcinoma control subject or higher than a certain threshold value (cutoff value), data can be collected for diagnosing the test subject as having a high possibility of having extrahepatic bile duct carcinoma, intrahepatic bile duct carcinoma, or gallbladder carcinoma.
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公开(公告)号：US20200255901A1
公开(公告)日：2020-08-13
申请号：US16322895
申请日：2017-08-03
Inventor： Ken YAMAGUCHI , Masatoshi KUSUHARA , Masakuni SERIZAWA , Tohru MOCHIZUKI , Keiichi OHSHIMA , Keiichi HATAKEYAMA , Kenichi URAKAMI , Shumpei OHNAMI , Yasuto AKIYAMA , Kouji MARUYAMA , Kengo INOUE , Yuji SHIMODA , Takeshi NAGASHIMA , Yoshinobu ISHIKAWA
IPC: C12Q1/6886 , G01N33/574
Abstract: An object of the present invention is to provide a method for predicting a risk of developing cancer. DNA samples were prepared from blood and cancer tissues of 2480 cancer patients and analyzed for the nucleotide sequences of exon regions using NGS. As a result, among the cancer patients, 7 patients were confirmed to have D49H mutation or A159D mutation which is a germ cell mutation.
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