公开(公告)号：US20140023993A1

公开(公告)日：2014-01-23

申请号：US14009473

申请日：2012-04-05

Applicant: Haishan Zeng ,  Harvey Lui ,  David McLean ,  Anthony Lee ,  Hequn Wang ,  Shuo Tang

Inventor： Haishan Zeng ,  Harvey Lui ,  David McLean ,  Anthony Lee ,  Hequn Wang ,  Shuo Tang

IPC: A61B5/00 ,  A61N5/06 ,  A61B18/18

CPC classification number: A61B5/0064 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/4836 ,  A61B5/6831 ,  A61B5/6832 ,  A61B18/18 ,  A61N5/06 ,  A61N5/0616 ,  G01J3/0218 ,  G01J3/027 ,  G01J3/44 ,  G02B21/0052 ,  G02B21/0076 ,  G02B21/06

Abstract: A multiphoton microscope is provided. The microscope includes: an excitation source for providing an optical excitation beam at an excitation wavelength λ; a scanner for scanning the excitation beam on a sample; an objective for irradiating the sample with the excitation beam scanned by the scanner and for collecting an emission beam from the sample; a first detector for detecting a plurality of multiphoton signals; and an emission light path allowing transmission from the objective to the first detector a wavelength band limited to greater than or equal to λ/2 and less than λ, wherein the plurality of multiphoton signals have wavelengths within the wavelength band; wherein the plurality of multiphoton signals comprises a first multiphoton signal and a second multiphoton signal of different types. Fast image capture rate multiphoton microscopes for in vivo imaging, as well as photothermolysis methods using the microscopes are also provided.

Abstract translation: 提供多光子显微镜。 显微镜包括：用于在激发波长λ下提供光学激发光束的激发源; 用于扫描样品上的激发光束的扫描器; 用扫描仪扫描的激发光束照射样品并收集来自样品的发射光束的目的; 用于检测多个多光子信号的第一检测器; 以及允许从所述物镜传输到所述第一检测器的发射光路，所述波长带被限制在大于或等于λ/ 2且小于λ的波长带，其中所述多个多光子信号具有所述波长带内的波长; 其中所述多个多光子信号包括不同类型的第一多光子信号和第二多光子信号。 还提供用于体内成像的快速图像捕获率多光子显微镜以及使用显微镜的光热解方法。

公开(公告)号：US20130335729A1

公开(公告)日：2013-12-19

申请号：US13966447

申请日：2013-08-14

Applicant: CRESCENT DIAGNOSTICS (IRELAND) LIMITED

Inventor： Mark Robert Towler

IPC: A61B5/00 ,  G01J3/44

CPC classification number: A61B5/0075 ,  A61B5/0059 ,  A61B5/441 ,  A61B5/4504 ,  A61B5/4509 ,  G01J3/44

Abstract: Methods of diagnosing bone disease such as osteoporosis are provided. The methods comprise detecting changes in the physical or chemical structure of a keratinized tissue as correlates of disease. The methods include detecting changes in the hardness, modulus, or level of sulfur bonding, particularly the level of disulfide bonding, in a keratinized tissue sample such as nail, hair, or skin. Changes in these variables serve as diagnostic markers of bone diseases that are associated with changes in bone elasticity and bone density.

Abstract translation: 提供诊断骨质疏松症等骨疾病的方法。 所述方法包括检测角质化组织的物理或化学结构的变化作为疾病的相关性。 所述方法包括检测角化组织样品如指甲，毛发或皮肤中的硫键的硬度，模量或水平的变化，特别是二硫键的水平。 这些变量的变化用作与骨骼弹性和骨密度变化相关的骨骼疾病的诊断标记。

公开(公告)号：US20130321801A1

公开(公告)日：2013-12-05

申请号：US13983451

申请日：2012-02-03

Applicant: Robert J. Levis ,  Johanan H. Odhner

Inventor： Robert J. Levis ,  Johanan H. Odhner

IPC: G01J3/44

CPC classification number: G01J3/44 ,  G01J3/10 ,  G01N21/65 ,  G01N2021/655

Abstract: In apparatus and methods of Raman spectroscopy in air, a target region is excited by a laser pump pulse exceeding the critical power for self-focusing in air and having a duration after self-focusing of 15 fs or less. A laser probe pulse having a duration in the range of 200 fs to 100 ps and an energy of at least 20 μJ is directed at the excited target region. Stimulated Raman scattering from the interaction between the excited target region and the laser probe pulse is detected. The target region can be outside the spectrometer, with ambient air in between used for the self-focusing.

Abstract translation: 在空气中的拉曼光谱仪的装置和方法中，目标区域被激光泵浦脉冲激发，该激光泵脉冲超过空气中的自聚焦的临界功率，并且具有15fs或更小的自聚焦之后的持续时间。 具有200fs至100ps范围内的持续时间和至少20μJ的能量的激光探针脉冲指向激发的目标区域。 检测激发目标区域与激光探头脉冲之间的相互作用引起的受激拉曼散射。 目标区域可以在光谱仪外部，其间的环境空气用于自聚焦。

公开(公告)号：US08582096B2

公开(公告)日：2013-11-12

申请号：US12969295

申请日：2010-12-15

Applicant: Zhongping Chen ,  Gangjun Liu ,  Mihael Balu ,  Bruce Tromberg ,  Eric Olaf Potma

Inventor： Zhongping Chen ,  Gangjun Liu ,  Mihael Balu ,  Bruce Tromberg ,  Eric Olaf Potma

IPC: G01J3/44

CPC classification number: G01J3/44 ,  A61B1/00165 ,  A61B1/043 ,  A61B5/0066 ,  A61B5/0084 ,  A61B5/02007 ,  A61B5/441 ,  G01J3/02 ,  G01J3/0218 ,  G01J3/0297 ,  G01N21/65 ,  G01N2021/653 ,  G01N2201/08 ,  G01N2201/0866

Abstract: A fiber-delivered probe suitable for CARS imaging of thick tissues is practical. The disclosed design is based on two advances. First, a major problem in CARS probe design is the presence of a very strong anti-Stokes component in silica delivery fibers generated through a FWM process. Without proper spectral filtering, this component affects the CARS image from the tissue sample. The illustrated embodiments of the invention efficiently suppress this spurious anti-Stokes component through the use of a separate fiber for excitation delivery and for signal detection, which allows the incorporation of dichroic optics for anti-Stokes rejection. Second, the detection of backscattered CARS radiation from the sample is optimized by using a large core multi mode fiber in the detection channel. This scheme produces high quality CARS images free of detector aperture effects. Miniaturization of this fiber-delivered probe results in a practical handheld probe for clinical CARS imaging.

Abstract translation: 适用于厚组织CARS成像的纤维输送探针是实用的。 所公开的设计基于两个进展。 首先，CARS探针设计中的一个主要问题是在通过FWM工艺生成的二氧化硅输送纤维中存在非常强的反斯托克斯组分。 没有适当的光谱过滤，该组件会影响来自组织样本的CARS图像。 本发明的所示实施例通过使用用于激发传递和信号检测的单独光纤来有效地抑制这种寄生的反斯托克斯分量，这允许并入用于反斯托克斯抑制的二向色光学器件。 其次，通过在检测通道中使用大型核心多模光纤，优化了样品背散射CARS辐射的检测。 该方案可生成高品质的CARS图像，无需检测器孔径效果。 该光纤传送探针的小型化导致临床CARS成像的实用手持式探头。

公开(公告)号：US08537354B2

公开(公告)日：2013-09-17

申请号：US12931868

申请日：2011-02-11

Applicant: John Maier ,  Michael Fuhrman

Inventor： John Maier ,  Michael Fuhrman

IPC: G01J3/44

CPC classification number: G01J3/02 ,  G01J3/0297 ,  G01J3/44 ,  G01N21/276 ,  G01N21/65 ,  G01N2201/1293

Abstract: A system and method for providing an instrument response correction. A sample is illuminated to generate a first plurality and a second plurality of interacted photons. The first plurality of interacted photons may be detected by a dispersive spectrometer to generate a reference spectrum representative of the sample. The second plurality of interacted photons may be passed through a tunable filter and detected using an imaging detector to generate at least one hyperspectral image. This hyperspectral image may comprise a Raman hyperspectral image or an infrared hyperspectral image. A system may comprise an illumination source, a collection optics, a dispersive spectrometer, a fiber optic, a tunable filter, and an imaging detector.

Abstract translation: 一种用于提供仪器响应校正的系统和方法。 照射样品以产生第一多个和第二多个相互作用的光子。 可以通过色散光谱仪检测第一组多个相互作用的光子，以产生代表样品的参考光谱。 可以将第二多个相互作用的光子通过可调滤光器并使用成像检测器进行检测以产生至少一个高光谱图像。 该高光谱图像可以包括拉曼高光谱图像或红外光谱图像。 系统可以包括照明源，收集光学器件，分散光谱仪，光纤，可调谐滤光器和成像检测器。

公开(公告)号：US20130222798A1

公开(公告)日：2013-08-29

申请号：US13757411

申请日：2013-02-01

Applicant: Sony Corporation

Inventor： Suguru Dowaki ,  Kazuhiro Nakagawa ,  Eriko Matsui

IPC: G01J3/44

CPC classification number: G01J3/44 ,  G01N21/65

Abstract: The present disclosure provides a method of measuring a Raman scattered light which is capable of detecting a Raman scattered light derived from a specimen at a high sensitivity, and a container for a Raman scattered light measurement specimen for use therein. The method of measuring a Raman scattered light includes radiating an exciting light to a specimen on a sheet member made of a material different from a material of an accommodating section and disposed within the accommodating section, thereby detecting a Raman scattered light.

Abstract translation: 本公开内容提供了一种测量能够以高灵敏度检测来自样本的拉曼散射光的拉曼散射光的测量方法和用于其中的拉曼散射光测量样本的容器。 测量拉曼散射光的方法包括：将激励光照射到由不同于容纳部的材料的材料制成的片材上，并设置在容纳部内，从而检测拉曼散射光。

公开(公告)号：US08514394B2

公开(公告)日：2013-08-20

申请号：US12853222

申请日：2010-08-09

Applicant: Wei Yang ,  Ming Chai ,  Charlie Zhang ,  Patrick Yang

Inventor： Wei Yang ,  Ming Chai ,  Charlie Zhang ,  Patrick Yang

IPC: G01J3/28

CPC classification number: G01J3/44 ,  G01J3/02 ,  G01J3/0208 ,  G01J3/021 ,  G01J3/0235 ,  G01J3/0291 ,  G01J3/1804

Abstract: A spectrograph having multiple excitation wavelength ranges is disclosed. The spectrograph can include a wavelength switching mechanism to switch between different wavelength ranges in accordance with the wavelength of an incoming light signal. The wavelength switching mechanism can include multiple optical assemblies (or elements) corresponding to the different wavelength ranges for processing the incoming light signal. The mechanism can also include a switching component for switching the optical assemblies to align the appropriate assembly with the incoming light signal. Each optical assembly can include one or more transmission gratings to disperse the incoming light signal into multiple wavelengths within a particular wavelength range and a reflecting mirror proximate to the grating(s) to reflect the wavelengths of light back through the grating(s) to photodetectors for measuring to wavelengths to generate a light spectrum. The spectrograph can be used in Raman spectroscopy.

Abstract translation: 公开了具有多个激发波长范围的光谱仪。 光谱仪可以包括根据入射光信号的波长在不同波长范围之间切换的波长切换机构。 波长切换机构可以包括对应于用于处理入射光信号的不同波长范围的多个光学组件（或元件）。 该机构还可以包括用于切换光学组件以将适当的组件与入射光信号对准的切换部件。 每个光学组件可以包括一个或多个透射光栅以将入射光信号分散在特定波长范围内的多个波长和靠近光栅的反射镜，以将光的波长反射回光栅到光电检测器 用于测量波长以产生光谱。 该光谱仪可用于拉曼光谱。

公开(公告)号：US20130182249A1

公开(公告)日：2013-07-18

申请号：US13825152

申请日：2011-09-20

Applicant: Sharon M. Weiss ,  Yang Jiao ,  Judson D. Ryckman ,  Peter N. Ciesielski ,  G. Kane Jennings

Inventor： Sharon M. Weiss ,  Yang Jiao ,  Judson D. Ryckman ,  Peter N. Ciesielski ,  G. Kane Jennings

IPC: G01J3/02 ,  B21D22/00 ,  G01J3/44

CPC classification number: G01J3/0267 ,  B21D22/00 ,  B82Y30/00 ,  B82Y40/00 ,  G01J3/44 ,  G01N21/658

Abstract: Provided are patterned nanoporous gold (“P-NPG”) films that may act as at least one of an effective and stable surface-enhanced Raman scattering (“SERS”) substrate. Methods of fabricating the P-NPG films using a low-cost stamping technique are also provided. The P-NPG films may provide uniform SERS signal intensity and SERS signal intensity enhancement by a factor of at least about 1×107 relative to the SERS signal intensity from a non-enhancing surface.

Abstract translation: 提供可以作为有效和稳定的表面增强拉曼散射（“SERS”）底物中的至少一种的图案化纳米多孔金（“P-NPG”）膜。 还提供了使用低成本冲压技术制造P-NPG膜的方法。 相对于来自非增强表面的SERS信号强度，P-NPG膜可以提供均匀的SERS信号强度和SERS信号强度增强因子至少约1×10 7。

公开(公告)号：US20130155402A1

公开(公告)日：2013-06-20

申请号：US13819397

申请日：2011-08-31

Applicant: Ian D. Walton ,  William E. Doering ,  Michael J. Natan

Inventor： Ian D. Walton ,  William E. Doering ,  Michael J. Natan

IPC: G01J3/28 ,  G01J3/44

CPC classification number: G01J3/28 ,  G01J3/0208 ,  G01J3/0218 ,  G01J3/0272 ,  G01J3/0291 ,  G01J3/0294 ,  G01J3/2803 ,  G01J3/44 ,  G01N21/85 ,  G01N21/8901 ,  G07D7/1205

Abstract: An inline spectroscopic reader having a light source, one or more optics heads, a spectrometer and a data processing system in digital communication with the spectrometer detector. The optics heads include transmission optics providing for the illumination of a target with light from the light source and detection optics providing for the collection of light from the target. Typically, the target is moving with respect to the optics head during spectroscopic interrogation. The spectroscopic reader is thus an inline reader well suited to provide spectrum based production or analytical decision making in real time as the target moves along a production or analysis line. Also disclosed are methods including the steps of illuminating a target with light from a light source; collecting light from the target; obtaining a digitized spectrum with a spectrometer; extracting information content from the digitized spectrum; and basing a contemporaneous process decision upon the information content.

Abstract translation: 具有光源，一个或多个光学头，光谱仪和与光谱仪检测器进行数字通信的数据处理系统的在线分光镜读取器。 光学头包括传输光学器件，用于提供来自光源的光的目标照明和用于从目标物体收集光的检测光学器件。 通常，目标在光谱询问期间相对于光学头移动。 因此，光谱阅读器是一种在线阅读器，非常适合随着目标沿生产或分析线路的移动而实时提供基于频谱的生产或分析决策。 还公开了包括用来自光源的光照射目标的步骤的方法; 从目标收集光; 用光谱仪获得数字化光谱; 从数字化频谱中提取信息内容; 并对信息内容进行同时代的过程决策。

公开(公告)号：US20130063722A1

公开(公告)日：2013-03-14

申请号：US13608539

申请日：2012-09-10

Applicant: William B. SPARKS

Inventor： William B. SPARKS

IPC: G01J3/447

CPC classification number: G01J3/447 ,  G01J3/44

Abstract: A single-shot real-time spectropolarimeter for use in astronomy and other sciences that captures and encodes some or all of the Stokes polarization parameters simultaneously using only static, robust optical components with no moving parts is described. The polarization information is encoded onto the spectrograph at each wavelength along the spatial dimension of the 2D output data array. The varying embodiments of the concept include both a two-Stokes implementation (in which any two of the three Stokes polarization parameters are measured) and a full Stokes implementation (in which all three of the Stokes polarization parameters are measured), each of which is provided in either single beam or dual beam forms.

Abstract translation: 描述了用于天文学和其他科学的单次实时分光偏振计，其仅使用没有移动部件的静态，坚固的光学部件同时捕获和编码部分或全部斯托克斯极化参数。 偏振信息沿着2D输出数据阵列的空间维度被编码在每个波长的光谱仪上。 该概念的变化实施例包括双斯托克斯实现（其中测量三个斯托克斯极化参数中的任何两个）和全斯托克斯实现（其中测量所有三个斯托克斯极化参数），每个都是 以单梁或双梁形式提供。