公开(公告)号：US20040196556A1

公开(公告)日：2004-10-07

申请号：US10771413

申请日：2004-02-05

Inventor： Gregory G. Cappiello

IPC: G02B005/18

CPC classification number: G02B6/2938 ,  G01J3/02 ,  G01J3/0208 ,  G01J3/0286 ,  G01J3/14 ,  G01J3/18 ,  G01J3/2803 ,  G01J3/36 ,  G02B5/18 ,  G02B6/29307 ,  G02B6/2931 ,  G02B6/3807 ,  G02B7/008 ,  G02B7/028 ,  G02B27/1073 ,  G02B27/1086 ,  H04B10/077 ,  H04B10/07955 ,  H04J14/02 ,  H04J14/0206 ,  H04J14/0209 ,  H04J14/0216

Abstract: A diffraction grating and devices employing same are disclosed. In one particular exemplary embodiment, the present invention may be realized as a diffraction grating comprising a reflective material having a blazed surface with a blaze angle between about 33 degrees and about 41 degrees, and an optically transmissive material disposed adjacent the reflective material having an index of refraction (n), wherein the blazed surface of the reflective material has approximately (350null30)*n number of grooves per millimeter.

Abstract translation: 公开了衍射光栅和采用它的器件。 在一个具体的示例性实施例中，本发明可以被实现为包括具有闪耀角度在约33度和约41度之间的闪耀表面的反射材料的衍射光栅，以及邻近反射材料设置的透光材料，其具有指数 （n），其中反射材料的闪耀表面具有每毫米大约（350±30）个数量的凹槽。

公开(公告)号：US06750457B2

公开(公告)日：2004-06-15

申请号：US09942472

申请日：2001-08-29

Applicant: David M. Heffelfinger ,  Aram P. Schiffman ,  Bala S. Manian

Inventor： David M. Heffelfinger ,  Aram P. Schiffman ,  Bala S. Manian

IPC: G01N2100

CPC classification number: G01N21/6452 ,  G01J3/10 ,  G01J3/14 ,  G01J3/36 ,  G01J3/4406 ,  G01N27/44721 ,  G01N2021/6463

Abstract: An apparatus and method in which illumination light and collected emitted light share a pathway and subsequently are physically separated. The optical configuration is designed such that at the point of separation, the illumination light is at has a smaller cross sectional area than the collected light. Collected light is directed away from the pathway of the illumination light and to detection optics. This configuration is adaptable to illumination and light collection across a broad wavelength spectrum. This configuration is adaptable to scanning in a limited depth of field to allow high throughput optical analysis of samples.

Abstract translation: 照明光和收集的发射光共享通路并随后物理分离的装置和方法。 光学构造被设计成使得在分离点处，照明光具有比收集的光更小的横截面面积。 收集的光被引导离开照明光的路径和检测光学器件。 该配置适用于宽波长光谱下的照明和光采集。 该配置适用于在有限的景深中的扫描，以允许样品的高通量光学分析。

公开(公告)号：US06747738B2

公开(公告)日：2004-06-08

申请号：US10188160

申请日：2002-07-01

Applicant: David J. Knapp

Inventor： David J. Knapp

IPC: G01J306

CPC classification number: G01J3/14 ,  H04N9/045

Abstract: An optical system (20) provides a variable dispersion that helps to collect multi-spectral information on an object within the field of view. The system (20) includes at least two sets (24, 26) of optical elements (42x 42y, 44x, 44y) that have minimal deviation of a center wavelength and a non-zero deviation of at least one other wavelength. By rotating the sets of optical elements (24, 26) relative to one another or together, the degree of wavelength dispersion and the direction of the wavelength dispersion can be varied, respectively. By selectively rotating the sets of optical elements, the system also can be operated in a non-dispersed “white light” mode with no net dispersion at the image plane.

公开(公告)号：US20030133109A1

公开(公告)日：2003-07-17

申请号：US10047156

申请日：2002-01-17

Inventor： James E. Murguia

IPC: G01J003/28

CPC classification number: G01J3/14 ,  G01J3/2823

Abstract: Tomographic approaches to hyperspectral imaging, such as CTHIS1 (Chromotomographic Hyperspectral Imaging Sensor), can eliminate the need for the slit, filter, or resonant cavity and substantially increase the optical throughput of the system. These systems capture a large fraction of the photon energy from the entire spectral band over the entire frame time. CTHIS uses a rotating direct view prism as the dispersing element, consequently, the extended image must be reconstructed from the blurred measured data. Only radiation from monochromatic sources such as LEDs, LASERs and signals with high spectral definition, such as the flames of chemical reactions, remain un-blurred in passing through the prism. Thus, the position, wavelength, and temporal evolution of LEDs, LASERs and certain flames can be easily identified in a wide field of view with minimal signal processing.

Abstract translation: CTHIS1（Chromotomographic Hyperspectral Imaging Sensor，高光谱成像传感器）可以消除对缝隙，滤波器或谐振腔的需求，并大大增加系统的光通量。 这些系统在整个帧时间内从整个光谱带捕获大部分光子能量。 CTHIS使用旋转直视棱镜作为分散元件，因此，扩展图像必须从模糊的测量数据重建。 来自诸如LED，LASER和具有高光谱清晰度的信号（例如化学反应的火焰）的单色源的辐射在通过棱镜时保持不模糊。 因此，LED，激光器和某些火焰的位置，波长和时间演变可以在宽视野内以最小的信号处理被容易地识别。

公开(公告)号：US06583865B2

公开(公告)日：2003-06-24

申请号：US09932844

申请日：2001-08-17

Applicant: David A. Basiji ,  William E. Ortyn

Inventor： David A. Basiji ,  William E. Ortyn

IPC: G01J314

CPC classification number: G06K9/00127 ,  G01J3/02 ,  G01J3/0208 ,  G01J3/0232 ,  G01J3/14 ,  G01J3/2823 ,  G01J3/2889 ,  G01N15/14 ,  G01N21/6458 ,  G01N2015/1497

Abstract: Light from an object moving through an imaging system is collected, dispersed, and imaged onto a time delay integration (TDI) detector that is inclined relative to an axis of motion of the object, producing a pixilated output signal. In one embodiment, the movement of the image object over the TDI detector is asynchronous with the movement of the output signal producing an output signal that is a composite of the image of the object at varying focal point along the focal plane. In another embodiment, light from the object is periodically incident on the inclined TDI detector, producing a plurality of spaced apart images and corresponding output signals that propagate across the TDI detector. The inclined plane enables images of FISH probes or other components within an object to be produced at different focal points, so that the 3D spatial relationship between the FISH probes or components can be resolved.

公开(公告)号：US06556297B1

公开(公告)日：2003-04-29

申请号：US09724638

申请日：2000-11-28

Applicant: Gregory G. Cappiello

Inventor： Gregory G. Cappiello

IPC: G01J328

CPC classification number: H04J14/02 ,  G01J3/02 ,  G01J3/0208 ,  G01J3/0286 ,  G01J3/14 ,  G01J3/18 ,  G01J3/2803 ,  G02B5/18 ,  G02B6/2931 ,  G02B6/29385 ,  G02B6/29398 ,  G02B7/008 ,  G02B7/028 ,  G02B27/1073 ,  G02B27/108 ,  G02B27/1086 ,  G02B27/123 ,  G02B27/126 ,  H04B10/077 ,  H04B10/07955 ,  H04J14/0201

Abstract: A device for monitoring wavelength division multiplexed optical signals for use in an optical network and in an optical performance monitor. A device has a structure for supporting components of the device. An optical component is supported at one end of the structure for transmitting the optical signals. A diffraction grating is supported at an opposing end of the structure for diffracting the optical signals from the optical component. An optical sensor is supported in relation to the diffraction grating by the structure for monitoring the optical signals. A telephoto lens assembly is supported by the structure and disposed between the optical sensor and the diffraction grating, the lens assembly having a focal length for focusing the optical signals in relation to the optical sensor. Thermal effects on the structure are balanced against thermal effects on the lens assembly. A prism is disposed between the lens assembly and diffraction grating. The prism is configured to anamorphically compress the diffracted optical signals. Thermal effects on the diffraction grating are balanced against thermal effects on the lens and prism.

Abstract translation: 一种用于监视在光网络和光学性能监视器中使用的波分复用光信号的装置。 装置具有用于支撑装置的部件的结构。 光学部件被支撑在用于传输光信号的结构的一端。 衍射光栅被支撑在用于衍射光学部件的光学信号的结构的相对端。 通过用于监测光信号的结构，相对于衍射光栅支撑光学传感器。 长焦透镜组件由该结构支撑并且设置在光学传感器和衍射光栅之间，透镜组件具有用于相对于光学传感器聚焦光学信号的焦距。 对结构的热效应与透镜组件的热效应相平衡。 棱镜设置在透镜组件和衍射光栅之间。 棱镜被配置成变形地压缩衍射的光信号。 对衍射光栅的热效应与透镜和棱镜上的热效应相平衡。

公开(公告)号：US20030044967A1

公开(公告)日：2003-03-06

申请号：US09942472

申请日：2001-08-29

Inventor： David M. Heffelfinger ,  Aram P. Schiffman ,  Bala S. Manian

IPC: C12M001/34 ,  G01J003/42 ,  G01J003/427

CPC classification number: G01N21/6452 ,  G01J3/10 ,  G01J3/14 ,  G01J3/36 ,  G01J3/4406 ,  G01N27/44721 ,  G01N2021/6463

Abstract: An apparatus and method in which illumination light and collected emitted light share a pathway and subsequently are physically separated. The optical configuration is designed such that at the point of separation, the illumination light is at has a smaller cross sectional area than the collected light. Collected light is directed away from the pathway of the illumination light and to detection optics. This configuration is adaptable to illumination and light collection across a broad wavelength spectrum. This configuration is adaptable to scanning in a limited depth of field to allow high throughput optical analysis of samples.

Abstract translation: 照明光和收集的发射光共享通路并随后物理分离的装置和方法。 光学构造被设计成使得在分离点处，照明光具有比收集的光更小的横截面面积。 收集的光被引导离开照明光的路径和检测光学器件。 该配置适用于宽波长光谱下的照明和光采集。 该配置适用于在有限的景深中的扫描，以允许样品的高通量光学分析。

公开(公告)号：US20030020908A1

公开(公告)日：2003-01-30

申请号：US10175543

申请日：2002-06-18

Applicant: Amnis Corporation

Inventor： Keith L. Frost ,  James K. Riley ,  David A. Basiji ,  William E. Ortyn

IPC: G01N021/64

CPC classification number: G01J3/10 ,  G01J3/14 ,  G01J3/28 ,  G01J3/4406 ,  G01N15/147 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/6456 ,  G01N2015/1472 ,  G01N2015/1486 ,  G01N2015/1497 ,  G01N2021/6423 ,  G01N2021/6441 ,  G01N2201/1293

Abstract: In an instrument generating images from the fluorescent emissions of a plurality of fluorescent dyes carried by objects in a flow stream, spectral dispersion is used to expand the images of the objects along one axis of a two-dimensional photosensor array according to emission wavelength. The dispersion is unable to completely separate the emissions of a plurality of dyes because the emission spectra of the dyes overlap in wavelength. The method of the present invention accomplishes accurate estimation of the intensity of the light received from each of a plurality of dyes by decomposing the two dimensional spectrally dispersed images into a set of characteristic parameters using either an iterative curve fitting optimization method or a linear algebraic method.

Abstract translation: 在从由流体中的物体携带的多个荧光染料的荧光发射产生图像的仪器中，使用光谱色散来根据发射波长来扩展沿着二维光电传感器阵列的一个轴的物体的图像。 由于染料的发射光谱在波长上重叠，所以色散不能完全分离多种染料的发射。 本发明的方法通过使用迭代曲线拟合优化方法或线性代数方法将二维光谱分散图像分解成一组特征参数，从而准确估计从多种染料中的每一种接收的光的强度 。

公开(公告)号：US06485150B1

公开(公告)日：2002-11-26

申请号：US09898778

申请日：2001-07-03

Applicant: Ronald G. Driggers ,  Ellis E. Burroughs, Jr. ,  Donald N. Williams ,  Charles S. Bendall

Inventor： Ronald G. Driggers ,  Ellis E. Burroughs, Jr. ,  Donald N. Williams ,  Charles S. Bendall

IPC: G02B504

CPC classification number: G02B27/126 ,  G01J3/04 ,  G01J3/10 ,  G01J3/12 ,  G01J3/14 ,  G01J2003/1282 ,  G01J2003/1286 ,  G02B27/1086

Abstract: A tunable spectral source includes an enclosure having first and second apertures; an optical dispersive element positioned in the enclosure; and multiple pixel source elements that are individually controllable for selectively directing one or more broadband light signals through the first aperture to irradiate the optical dispersive element. Each of the broadband light signals irradiates the optical dispersive element at a unique angle of incidence. The optical dispersive element disperses the broadband light signals into spectral component signals at dispersion angles that are dependent upon the angle of incidence of each broadband light signal that irradiates the optical dispersive element. The portions of the spectral component signals that are emitted through the second aperture are determined by selecting one or more particular pixel source elements to irradiate the optical dispersive element.

Abstract translation: 可调谐光谱源包括具有第一和第二孔的外壳; 位于外壳中的光学分散元件; 以及可独立控制的多个像素源元件，用于选择性地引导一个或多个宽带光信号穿过第一孔径以照射光学色散元件。 每个宽带光信号以独特的入射角照射光学色散元件。 光学色散元件将宽带光信号分散成分散角的光谱分量信号，这取决于照射光学色散元件的每个宽带光信号的入射角。 通过选择一个或多个特定像素源元件来照射光学色散元件来确定通过第二孔径发射的光谱分量信号的部分。

公开(公告)号：US06377344B2

公开(公告)日：2002-04-23

申请号：US09295554

申请日：1999-04-21

Applicant: Guenter Schoeppe

Inventor： Guenter Schoeppe

IPC: G01J330

CPC classification number: G01J3/02 ,  G01J3/0205 ,  G01J3/021 ,  G01J3/14 ,  G01J3/36 ,  G01J2003/1286 ,  G02B21/0064

Abstract: A device for the adjustable coupling of wavelengths or wavelength regions into the illumination beam path of a microscope, preferably in the beam path of a confocal microscope, comprising at least one dispersive element for wavelength separation of the illumination light and at least one at least partially reflecting element arranged in the wavelength-separated portion of the illumination light for reflecting back a wavelength region in the direction of the microscope illumination, and a device for the adjustable detection of object light coming from an illuminated object, preferably in a microscope beam path, comprising at least one dispersive element for wavelength separation of the object light and means arranged in the wavelength-separated portion of the object light for the adjustable stopping down or cutting out of at least one wavelength region and deflection in the direction of at least one detector.

Abstract translation: 用于将波长或波长区域可调节耦合到显微镜的照明光束路径中的装置，优选地在共焦显微镜的光束路径中，包括用于照明光的波长分离的至少一个色散元件和至少部分地至少部分地 布置在照明光的波长分离部分中的反射元件，用于反射显微照明方向上的波长区域;以及用于可调节地检测来自被照射物体的物体光，优选在显微镜光路中的装置， 包括用于对象光的波长分离的至少一个色散元件和布置在物体光的波长分离部分中的装置，用于可调节地停止或切除至少一个波长区域并沿着至少一个检测器的方向偏转 。