公开(公告)号：US20140234933A1

公开(公告)日：2014-08-21

申请号：US14159044

申请日：2014-01-20

Applicant: Danisco US Inc.

Inventor： Nigel DUNN-COLEMAN ,  Frits GOEDEGEBUUR ,  Michael WARD ,  Jian YAO

IPC: C12N9/42 ,  C12P7/14

CPC classification number: C12N9/2437 ,  C11D3/38 ,  C11D3/38636 ,  C11D3/38645 ,  C12N9/2445 ,  C12N15/1137 ,  C12N2310/11 ,  C12P7/10 ,  C12P7/14 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C12Y302/01004 ,  Y02E50/16 ,  Y02E50/17

Abstract: The present invention provides a novel endoglucanase nucleic acid sequence, designated egl7, and the corresponding EGVII amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVII, recombinant EGVII proteins and methods for producing the same.

Abstract translation: 本发明提供了一种命名为egl7的新型内切葡聚糖酶核酸序列和相应的EGVII氨基酸序列。 本发明还提供了包含编码EGVII的核酸序列，重组EGVII蛋白的表达载体和宿主细胞及其制备方法。

公开(公告)号：US20140209092A1

公开(公告)日：2014-07-31

申请号：US14130878

申请日：2012-07-09

Applicant: William F. McDonald ,  Shannon Scott Urban ,  Jason L. Martin

Inventor： William F. McDonald ,  Shannon Scott Urban ,  Jason L. Martin

IPC: C13K13/00 ,  C13K1/02

CPC classification number: C13K13/002 ,  C12P2201/00 ,  C12P2203/00 ,  C13K1/02 ,  C13K13/007 ,  Y02E50/16

Abstract: Methods and systems for pretreating lignocellulosic biomass are disclosed. An acid solution between 1% to 1.6% sulfuric acid is applied to the biomass. The biomass is subjected to an elevated temperature to cause the production of xylose, glucose, and furfural. Adjustments to temperature, acid concentration, and time can generate at least 80% or 90% of theoretical xylose, 45% or 50% of the theoretical glucose, and less than 4000 ppm of furfural in the xylose liquor. A portion of the resulting xylose liquor may be separated from the glucan solids. The xylose liquor, still highly acidic, can be recycled to reduce subsequent acid loading requirements. Makeup acid solution is added to the xylose liquor and subsequent biomass to ensure a proper solids to liquids ratio. The biomass is again treated to higher temperatures to yield sugars. The process may be repeated for each subsequent cycle.

Abstract translation: 公开了用于预处理木质纤维素生物质的方法和系统。 将1％至1.6％硫酸的酸溶液施用于生物质。 生物质经历升高的温度以产生木糖，葡萄糖和糠醛。 温度，酸浓度和时间的调整可以产生理论木糖的至少80％或90％，理论葡萄糖的45％或50％，和木糖液中的糠醛少于4000ppm。 所得木糖液体的一部分可以与葡聚糖固体分离。 仍然高度酸性的木糖液体可被再循环以减少随后的酸负荷需求。 将补充酸溶液加入到木糖液和随后的生物质中以确保适当的固体与液体的比例。 再次将生物质处理至较高温度以产生糖。 可以为每个后续循环重复该过程。

公开(公告)号：US20140193870A1

公开(公告)日：2014-07-10

申请号：US14201733

申请日：2014-03-07

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： William T. BEESON, IV ,  James H. DOUDNA CATE ,  Michael A. MARLETTA

IPC: C12P7/58

CPC classification number: C12P19/02 ,  A23K10/14 ,  A23K50/30 ,  A23K50/75 ,  C11D3/386 ,  C12N9/18 ,  C12P7/58 ,  C12P2201/00 ,  C12Y101/03004 ,  C12Y101/99018 ,  C12Y301/01017 ,  D21C5/00 ,  D21C5/005

Abstract: The present disclosure relates to hydrolysis of hexose-δ-lactones by use of an S. thermophile extracellular aldonolactonase. In particular the present disclosure relates to compositions including a S. thermophile extracellular aldonolactonase and methods of use thereof.

Abstract translation: 本公开内容涉及通过使用嗜热脂肪酶胞外aldonolactonase水解己糖-δ-内酯。 特别地，本公开涉及包含嗜热链球菌细胞外α-淀粉酶的组合物及其使用方法。

公开(公告)号：US08771480B2

公开(公告)日：2014-07-08

申请号：US13922641

申请日：2013-06-20

Applicant: Xyleco, Inc.

Inventor： Marshall Medoff

IPC: C10G65/02

CPC classification number: B01J19/08 ,  B01J19/081 ,  B01J19/085 ,  B01J2219/08 ,  B01J2219/0879 ,  C08B1/00 ,  C10G3/00 ,  C10G11/00 ,  C10G15/10 ,  C10G47/00 ,  C10G2300/1011 ,  C10L1/02 ,  C10L1/04 ,  C10L2200/0469 ,  C10L2270/02 ,  C10L2270/04 ,  C10L2290/02 ,  C10L2290/06 ,  C10L2290/26 ,  C10L2290/34 ,  C10L2290/36 ,  C10L2290/543 ,  C12P5/00 ,  C12P5/02 ,  C12P5/023 ,  C12P7/02 ,  C12P7/04 ,  C12P7/10 ,  C12P7/14 ,  C12P7/16 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C12P2203/00 ,  H01M8/1007 ,  H01M8/1013 ,  H01M8/16 ,  H01M8/22 ,  Y02E50/16 ,  Y02E50/343 ,  Y02P30/20 ,  Y02T50/678

Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce ethanol and/or butanol, e.g., by fermentation.

公开(公告)号：US20140154756A1

公开(公告)日：2014-06-05

申请号：US14092906

申请日：2013-11-27

Applicant: API Intellectual Property Holdings, LLC

Inventor： Kimberly NELSON ,  Theodora RETSINA ,  Vesa PYLKKANEN ,  Ryan O'CONNOR

IPC: D21C5/00

CPC classification number: C08B15/08 ,  C07G1/00 ,  C08B15/00 ,  C08B15/02 ,  C08B37/0057 ,  C08H8/00 ,  C08L1/02 ,  C12P7/04 ,  C12P7/10 ,  C12P7/14 ,  C12P7/16 ,  C12P7/28 ,  C12P7/46 ,  C12P7/56 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C12P2203/00 ,  C13K1/02 ,  D21C3/04 ,  D21C3/06 ,  D21C3/20 ,  D21C5/00 ,  D21C5/005 ,  D21C9/002 ,  D21C9/007 ,  D21C9/10 ,  D21C11/0007 ,  D21H11/18 ,  Y02E50/16 ,  Y02P40/44

Abstract: Processes disclosed are capable of converting biomass into high-crystallinity nanocellulose with surprisingly low mechanical energy input. In some variations, the process includes fractionating biomass with an acid (such as sulfur dioxide), a solvent (such as ethanol), and water, to generate cellulose-rich solids and a liquid containing hemicellulose and lignin; and mechanically treating the cellulose-rich solids to form nanofibrils and/or nanocrystals. The total mechanical energy may be less than 500 kilowatt-hours per ton. The crystallinity of the nanocellulose material may be 80% or higher, translating into good reinforcing properties for composites. The nanocellulose material may include nanofibrillated cellulose, nanocrystalline cellulose, or both. In some embodiments, the nanocellulose material is hydrophobic via deposition of some lignin onto the cellulose surface. Optionally, sugars derived from amorphous cellulose and hemicellulose may be separately fermented, such as to monomers for various polymers. These polymers may be combined with the nanocellulose to form completely renewable composites.

公开(公告)号：US20140154749A1

公开(公告)日：2014-06-05

申请号：US13902246

申请日：2013-05-24

Applicant: Xyleco, Inc.

Inventor： Marshall Medoff

IPC: C13K1/02 ,  C12P7/16 ,  C12P19/14 ,  C12P19/02 ,  C12P7/56 ,  C12P7/46 ,  C12P7/10 ,  C12P7/40

CPC classification number: C12P13/24 ,  A23K10/12 ,  A23K10/30 ,  A23K10/37 ,  A23K20/163 ,  A23K40/00 ,  A23K50/10 ,  A23K50/80 ,  A23L5/36 ,  A23L7/115 ,  A23V2002/00 ,  A61K8/97 ,  A61Q19/00 ,  B01D39/18 ,  C08H8/00 ,  C08J3/28 ,  C08L1/02 ,  C08L5/14 ,  C12P7/10 ,  C12P7/16 ,  C12P7/40 ,  C12P7/46 ,  C12P7/56 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/12 ,  C12P13/14 ,  C12P13/20 ,  C12P13/222 ,  C12P13/227 ,  C12P19/02 ,  C12P19/14 ,  C12P19/44 ,  C12P2201/00 ,  C12P2203/00 ,  C13K1/02 ,  D21C5/005 ,  D21C9/001 ,  Y02A40/818 ,  Y02E50/10 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/30 ,  Y02P60/877

Abstract: Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids.

Abstract translation: 加工生物质（例如，植物生物量，动物生物量，微生物和城市废物生物质）以产生有用的产物，例如食品和氨基酸。

公开(公告)号：US20140147907A1

公开(公告)日：2014-05-29

申请号：US14169174

申请日：2014-01-31

Applicant: XYLECO, INC.

Inventor： Marshall Medoff ,  Thomas Craig Masterman

IPC: C12P19/02

CPC classification number: C12P7/10 ,  C07H3/02 ,  C07H3/04 ,  C12N1/20 ,  C12N1/38 ,  C12P5/02 ,  C12P7/04 ,  C12P7/06 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/52 ,  C12P7/54 ,  C12P7/56 ,  C12P7/58 ,  C12P7/6409 ,  C12P19/02 ,  C12P2201/00 ,  C12P2203/00 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/343

Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to proceed ethanol and/or butanol, e.g., by fermentation.

Abstract translation: 加工生物质（例如，植物生物量，动物生物质和城市废物生物质）以产生有用的产物，例如燃料。 例如，系统可以使用原料，例如纤维素和/或木质纤维素材料，例如通过发酵来进行乙醇和/或丁醇。

公开(公告)号：US08728779B2

公开(公告)日：2014-05-20

申请号：US13312695

申请日：2011-12-06

Applicant: Marshall Medoff

Inventor： Marshall Medoff

IPC: C12P7/52 ,  C12P7/62 ,  C12P7/40 ,  H01J37/317 ,  H01J37/06 ,  C12P1/00 ,  C12P7/42

CPC classification number: C12P13/24 ,  A23K10/12 ,  A23K10/30 ,  A23K10/37 ,  A23K20/163 ,  A23K40/00 ,  A23K50/10 ,  A23K50/80 ,  A23L5/36 ,  A23L7/115 ,  A23V2002/00 ,  A61K8/97 ,  A61Q19/00 ,  B01D39/18 ,  C08H8/00 ,  C08J3/28 ,  C08L1/02 ,  C08L5/14 ,  C12P7/10 ,  C12P7/16 ,  C12P7/40 ,  C12P7/46 ,  C12P7/56 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/12 ,  C12P13/14 ,  C12P13/20 ,  C12P13/222 ,  C12P13/227 ,  C12P19/02 ,  C12P19/14 ,  C12P19/44 ,  C12P2201/00 ,  C12P2203/00 ,  C13K1/02 ,  D21C5/005 ,  D21C9/001 ,  Y02A40/818 ,  Y02E50/10 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/30 ,  Y02P60/877

Abstract: Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids.

公开(公告)号：US20140134668A1

公开(公告)日：2014-05-15

申请号：US14079015

申请日：2013-11-13

Applicant: UNIVERSITY OF TENNESSEE RESEARCH FOUNDATION

Inventor： NICOLE LABBE ,  BONNIE H. OWNLEY ,  KIMBERLY D. GWINN ,  NAIMA MOUSTAID-MOUSSA ,  DORIS H. D'SOUZA

IPC: C12Q1/18

CPC classification number: A61K36/899 ,  A01N37/06 ,  A01N65/00 ,  A01N65/40 ,  A01N65/44 ,  A23L33/105 ,  A23V2002/00 ,  A61K31/192 ,  A61K2236/33 ,  C10G3/00 ,  C10G2300/1014 ,  C10L1/02 ,  C10L2200/0469 ,  C10L2290/26 ,  C12P2201/00 ,  Y02P30/20 ,  A23V2200/10 ,  A23V2200/328

Abstract: Switchgrass is an increasingly important biofuel crop, but knowledge of switchgrass fungal pathogens is not extensive. The purpose of this research was to identify the fungal pathogens that decrease crop yield of switchgrass grown in Tennessee and to investigate a potential sustainable disease management strategy from a value-added by-product of the switchgrass biofuel conversion process. The specific objectives were 1) to identify and characterize prevalent fungal pathogens of switchgrass in Tennessee, 2) assess switchgrass seed produced in the United States for seedborne fungal pathogens, and 3) evaluate switchgrass extractives for antimicrobial activity against plant pathogens.

Abstract translation: 柳枝稷是一个越来越重要的生物燃料作物，但柳枝稷真菌病原体的知识并不广泛。 这项研究的目的是确定降低田纳西州生长柳枝稷的作物产量的真菌病原体，并从柳枝稷生物燃料转化过程的附加副产品中调查潜在的可持续疾病管理策略。 具体目标是1）确定田纳西州柳枝稷常见真菌病原体的特征，2）评估在美国生产的种子真菌病原体的柳枝稷种子，3）评估柳枝稷提取物对植物病原体的抗菌活性。

公开(公告)号：US08716537B2

公开(公告)日：2014-05-06

申请号：US13656892

申请日：2012-10-22

Applicant: Xyleco, Inc.

Inventor： Marshall Medoff

IPC: C07C27/04 ,  C07C27/00

CPC classification number: B01J19/085 ,  B01J19/081 ,  B01J19/121 ,  B01J19/123 ,  B01J19/126 ,  B01J2219/00006 ,  B01J2219/08 ,  B01J2219/0869 ,  B01J2219/0871 ,  B01J2219/0879 ,  B01J2219/089 ,  B02C23/18 ,  C07C27/00 ,  C07C27/04 ,  C07C27/24 ,  C08B1/00 ,  C12M45/00 ,  C12P7/10 ,  C12P19/00 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C13K1/02 ,  D21B1/02 ,  D21B1/063 ,  G21K5/00 ,  H01J37/3002 ,  Y02E50/16 ,  Y02E50/17

Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy or sugary materials, to produce ethanol and/or butanol, e.g., by fermentation.

Abstract translation: 加工生物质（例如，植物生物量，动物生物质和城市废物生物质）以产生有用的产物，例如燃料。 例如，系统可以使用诸如纤维素和/或木质纤维素材料和/或淀粉或糖类材料的原料来生产乙醇和/或丁醇，例如通过发酵。