公开(公告)号：US20210157115A1

公开(公告)日：2021-05-27

申请号：US17141137

申请日：2021-01-04

Applicant: University of Southern California

Inventor： Thai V. Truong ,  Sara Madaan ,  Daniel B. Holland ,  Scott E. Fraser

IPC: G02B21/00 ,  G02B21/36 ,  H04N19/597 ,  H04N13/254 ,  H04N13/111

Abstract: Volumetric imaging with selective volume illumination (SVI) using light field detection is provided using various systems and techniques. A volumetric imaging apparatus includes a light source configured to emit an illumination light that propagates via an illumination light path to illuminate a three-dimensional (3D) sample; and an optical system arranged with respect to the light source to receive a light field, which comes from the illuminated 3D sample. The light field propagates via a detection light path, and the light source, the optical system, or both, are configurable to perform SVI, which selects a volume of a 3D-confined illumination of the 3D sample based on the 3D sample to be illuminated and a light field detection (LFD) process to be applied. Further, the volume of the 3D-confined illumination is a selected 3D volume of the 3D sample to be particularly excited by the 3D-confined illumination for imaging.

公开(公告)号：US20180127785A1

公开(公告)日：2018-05-10

申请号：US15573732

申请日：2016-05-13

Applicant: University of Southern California

Inventor： Jason Junge ,  Timothy Hunt ,  Scott E. Fraser

IPC: C12N15/90 ,  C12N9/22 ,  C07K14/435 ,  C12N15/11

CPC classification number: C12N15/907 ,  C07K14/43504 ,  C07K2319/60 ,  C07K2319/80 ,  C07K2319/81 ,  C12N9/22 ,  C12N15/102 ,  C12N15/11 ,  C12N15/85 ,  C12N2310/20 ,  C12N2800/80

Abstract: Described herein are methods and compositions for genomic editing. Endonucleases for genomic editing involve inducing breaks in double stranded DNA, for which knock-ins are notoriously inefficient for relying on random integration of homologous DNA sequences into the break site by repair proteins. To address these issues, described herein are novel recombinant fusion proteins that actively recruit linear DNA inserts in closer proximity to the genomic cleavage site, increasing integration efficiency of large DNA fragments into the genome. Such improvements to genomic editing technology allow one to use lower linear DNA concentrations without sacrificing efficiency and can be further combined with other features, such as fluorescent protein reporting systems.

公开(公告)号：US20230203541A1

公开(公告)日：2023-06-29

申请号：US18088317

申请日：2022-12-23

Applicant: UNIVERSITY OF SOUTHERN CALIFORNIA

Inventor： Jason Junge ,  Timothy Hunt ,  Scott E. Fraser

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/85 ,  C12N15/10 ,  C07K14/435 ,  C12N15/11

CPC classification number: C12N15/907 ,  C12N9/22 ,  C12N15/85 ,  C12N15/102 ,  C07K14/43504 ,  C12N15/11 ,  C07K2319/60 ,  C07K2319/80 ,  C12N2310/20 ,  C07K2319/81 ,  C12N2800/80

Abstract: Described herein are methods and compositions for genomic editing. Endonucleases for genomic editing involve inducing breaks in double stranded DNA, for which knock-ins are notoriously inefficient for relying on random integration of homologous DNA sequences into the break site by repair proteins. To address these issues, described herein are novel recombinant fusion proteins that actively recruit linear DNA inserts in closer proximity to the genomic cleavage site, increasing integration efficiency of large DNA fragments into the genome. Such improvements to genomic editing technology allow one to use lower linear DNA concentrations without sacrificing efficiency and can be further combined with other features, such as fluorescent protein reporting systems.

公开(公告)号：US20230138764A1

公开(公告)日：2023-05-04

申请号：US17910305

申请日：2021-03-12

Applicant: University of Southern California

Inventor： Thai Truong ,  Kevin Keomanee-Dizon ,  Scott E. Fraser

IPC: G01N21/64 ,  G02B21/00 ,  G02B21/36 ,  G02B21/24

Abstract: Systems and methods are provided for increasing photon collection during imaging with a light-sheet fluorescence microscope. In one example, one or more adaptive optical elements may be positioned in a detection light path between a detection objective and an imaging sensor. A depth of field of the detection objective is adjusted as a function of a thickness of an excitation light-sheet used to illuminate a sample during imaging. As a result, the detection objective captures more fluorescence photons generated by light-sheet excitation.

公开(公告)号：US11535871B2

公开(公告)日：2022-12-27

申请号：US15573732

申请日：2016-05-13

Applicant: University of Southern California

Inventor： Jason Junge ,  Timothy Hunt ,  Scott E. Fraser

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/85 ,  C07K14/435 ,  C12N15/11 ,  C12N15/10

Abstract: Described herein are methods and compositions for genomic editing. Endonucleases for genomic editing involve inducing breaks in double stranded DNA, for which knock-ins are notoriously inefficient for relying on random integration of homologous DNA sequences into the break site by repair proteins. To address these issues, described herein are novel recombinant fusion proteins that actively recruit linear DNA inserts in closer proximity to the genomic cleavage site, increasing integration efficiency of large DNA fragments into the genome. Such improvements to genomic editing technology allow one to use lower linear DNA concentrations without sacrificing efficiency and can be further combined with other features, such as fluorescent protein reporting systems.

公开(公告)号：US11492657B2

公开(公告)日：2022-11-08

申请号：US16606038

申请日：2018-02-28

Applicant: University of Southern California

Inventor： Scott E. Fraser ,  Simon Restrepo ,  Joseph P. Dunham

IPC: C12Q1/682 ,  C12Q1/6834 ,  C12Q1/6841 ,  C12N15/11 ,  G01N21/64

Abstract: Described herein is a method to create dendritic biocompatible polymers from pairs of complementary dendritic nucleic acid monomers in a controlled manner, using polymerization triggers. The dendritic monomers are constituted of nucleic acids and an organic polymer capable of self-assembly. Each polymer contains approximately 200 dendrites that can be used to attach labels and constitute a biologically compatible signal amplification technology. Depending on the context this technology could be used to reveal the presence of a large variety of analytes such as specific nucleic acid molecules, small molecules, proteins, and peptides.

公开(公告)号：US10901193B2

公开(公告)日：2021-01-26

申请号：US16079979

申请日：2017-02-24

Applicant: University of Southern California

Inventor： Thai V. Truong ,  Sara Madaan ,  Daniel B. Holland ,  Scott E. Fraser

IPC: G02B21/00 ,  G02B21/36 ,  H04N19/597 ,  H04N13/254 ,  H04N13/111

Abstract: Volumetric imaging with selective volume illumination (SVI) using light field detection is provided using various systems and techniques. A volumetric imaging apparatus includes a light source configured to emit an illumination light that propagates via an illumination light path to illuminate a three-dimensional (3D) sample; and an optical system arranged with respect to the light source to receive a light field, which comes from the illuminated 3D sample. The light field propagates via a detection light path, and the light source, the optical system, or both; are configurable to perform SVI, which selects a volume of a 3D-confined illumination of the 3D sample based on the 3D sample to be illuminated and a light field detection (LFD) process to be applied. Further, the volume of the 3D-confined illumination is a selected 3D volume of the 3D sample to be particularly excited by the 3D-confined illumination for imaging.

公开(公告)号：US10856735B2

公开(公告)日：2020-12-08

申请号：US16465070

申请日：2017-11-27

Applicant: UNIVERSITY OF SOUTHERN CALIFORNIA

Inventor： Yu Chen ,  Scott E. Fraser

IPC: G01B9/02 ,  G06T5/00 ,  A61B3/10 ,  A61B3/12 ,  A61B3/14 ,  A61B3/15

Abstract: This disclosure relates to the field of Optical Coherence Tomography (OCT). This disclosure particularly relates to an OCT system that generates an image with improved quality. In one example, the OCT system may generate an improved Bscan image by using multiple shaping functions to shape the raw A-scans. In another example, the OCT system may generate the improved B-scan image by forming multiple apodization patterns on a detector and acquiring raw A-scans by using the apodization patterns. A better diagnosis of a health condition may be reached by using the improved images generated by the OCT system of this disclosure.

公开(公告)号：US20190064493A1

公开(公告)日：2019-02-28

申请号：US16079979

申请日：2017-02-24

Applicant: University of Southern California

Inventor： Thai V. Truong ,  Sara Madaan ,  Daniel B. Holland ,  Scott E. Fraser

IPC: G02B21/00 ,  G02B21/36 ,  H04N13/111 ,  H04N13/275 ,  H04N19/597 ,  H04N13/194 ,  H04N13/254

Abstract: Systems and techniques relating to optimizing volumetric imaging with selective volume illumination (SVI) using light field detection, in one aspect, include: a light source configured to emit an illumination light that propagates via an illumination light path to illuminate a three-dimensional (3D) sample; and an optical system arranged with respect to the light source to receive a light field, which comes from the illuminated 3D sample, wherein the light field propagates via a detection light path; wherein the light source, the optical system, or both, are configurable to select a volume of a 3D-confined illumination of the 3D sample based on the 3D sample to be illuminated and a light field detection (LFD) process to be applied.

公开(公告)号：US09763569B2

公开(公告)日：2017-09-19

申请号：US15104491

申请日：2015-02-04

Applicant: UNIVERSITY OF SOUTHERN CALIFORNIA ,  CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Jeffrey P. Fingler ,  Scott E. Fraser

IPC: A61B3/14 ,  A61B3/00 ,  A61B3/10 ,  A61B3/12 ,  A61B5/00 ,  G01B9/02

CPC classification number: A61B3/0025 ,  A61B3/0041 ,  A61B3/102 ,  A61B3/1233 ,  A61B5/7203 ,  G01B9/02076 ,  G01B9/02091 ,  G01B2290/65

Abstract: This disclosure relates to the field of Optical Coherence Tomography (OCT). This disclosure particularly relates to an OCT system with improved motion contrast. This disclosure particularly relates to motion contrast methods for such OCT systems. The OCT system of this disclosure may have a configuration that scans a physical object, which has a surface and a depth, with a beam of light that has a beam width and a direction; acquires OCT signals from the scan; forms at least one A-scan using the acquired OCT signals; forms at least one B-scan cluster set using the acquired OCT signals that includes at least two B-scan clusters that each include at least two B-scans. The B-scans within each B-scan cluster set are parallel to one another and parallel to the direction of the beam of light. The OCT system may have a configuration that calculates OCT motion contrast using the at least one B-scan cluster set. This OCT system may form and display an image of the physical object.