公开(公告)号：US20180170973A1

公开(公告)日：2018-06-21

申请号：US15887381

申请日：2018-02-02

Applicant: Kaneka Corporation

Inventor： Masakatsu Nishihachijyo ,  Keita Yamashita ,  Osamu Odawara ,  Takeyuki Tsuchidate ,  Masayuki Takano ,  Shinichi Yoshida

IPC: C07K14/31 ,  C07K1/22 ,  B01J20/24 ,  B01J20/286 ,  B01J20/32

CPC classification number: C07K14/31 ,  B01J20/24 ,  B01J20/286 ,  B01J20/3217 ,  B01J20/3278 ,  B01J2220/52 ,  C07K1/22 ,  C12N5/10 ,  C12N15/09 ,  C12P21/02

Abstract: A protein includes two or more amino acid sequences, wherein each amino acid sequence is derived from a sequence selected from the group consisting of SEQ ID NOs:1 to 5. The amino acid sequence closest to the N-terminus includes a substitution of an amino acid residue at a position corresponding to position 4 or 7 of SEQ ID NO: 5 with an amino acid residue other than Arg, and productivity of the protein in a transformant producing the protein is improved, as compared to productivity of a protein including the amino acid sequence without the substitution.

公开(公告)号：US09981205B2

公开(公告)日：2018-05-29

申请号：US14787428

申请日：2014-05-07

Applicant: HITACHI, LTD.

Inventor： Keisuke Shibuya ,  Yui Sugita ,  Ryouichi Haga ,  Masaru Nanba

IPC: B01D15/38 ,  B01J20/24 ,  B01J20/286 ,  B01J20/32 ,  B01J20/34 ,  C07K1/22

CPC classification number: B01D15/3809 ,  B01D15/3876 ,  B01J20/24 ,  B01J20/286 ,  B01J20/3272 ,  B01J20/3425 ,  B01J20/3475 ,  B01J20/3483 ,  B01J2220/58 ,  C07K1/22

Abstract: Provided is a purification device for purifying a useful substance (i.e., target substance) without using an acidic eluent. The purification device purifying the target substance comprises a column and a filler packed in the column. The filler contains a carrier, a temperature responsive polyamino acid bonded to the carrier, and a binding substance bonded to the temperature responsive polyamino acid and specifically bonding to the target substance.

公开(公告)号：US20180044374A1

公开(公告)日：2018-02-15

申请号：US15798784

申请日：2017-10-31

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Annika Forss ,  Mats Ander ,  Tomas Bjorkman ,  Hans Blom ,  Jesper Hansson ,  Gustav Rodrigo

IPC: C07K1/16 ,  B01D15/18 ,  B01J20/285 ,  B01D15/34 ,  C07K16/00 ,  B01J20/286

CPC classification number: C07K1/165 ,  B01D15/1807 ,  B01D15/1864 ,  B01D15/34 ,  B01J20/285 ,  B01J20/286 ,  C07K1/22 ,  C07K14/31 ,  C07K16/00 ,  C07K2317/21 ,  C07K2317/31 ,  C07K2319/30

Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising at least 15 mg/ml multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, wherein the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml; b) contacting a liquid sample comprising an immunoglobulin with the separation matrix; c) washing the separation matrix with a washing liquid; d) eluting the immunoglobulin from the separation matrix with an elution liquid; and e) cleaning the separation matrix with a cleaning liquid comprising at least 0.5 M NaOH.

公开(公告)号：US20170320977A1

公开(公告)日：2017-11-09

申请号：US15526512

申请日：2015-12-11

Applicant: ExxonMobil Research and Engineering Company

Inventor： Matthew W. HOLTCAMP ,  Matthew S. BEDOYA ,  Charles J. HARLAN ,  Quanchang LI ,  Machteld M.W. MERTENS

IPC: C08F10/02

CPC classification number: C08F10/02 ,  B01D15/00 ,  B01D53/02 ,  B01D53/0462 ,  B01D53/047 ,  B01D53/228 ,  B01D2253/106 ,  B01D2253/204 ,  B01D2253/306 ,  B01D2253/308 ,  B01D2253/311 ,  B01D2256/245 ,  B01D2257/102 ,  B01D2257/302 ,  B01D2257/304 ,  B01D2257/306 ,  B01D2257/404 ,  B01D2257/504 ,  B01D2257/702 ,  B01D2257/80 ,  B01J20/06 ,  B01J20/08 ,  B01J20/10 ,  B01J20/103 ,  B01J20/264 ,  B01J20/28011 ,  B01J20/28057 ,  B01J20/28069 ,  B01J20/28078 ,  B01J20/286 ,  B01J20/3042 ,  B01J20/3236 ,  B01J20/3238 ,  B01J23/42 ,  B01J23/44 ,  B01J29/0325 ,  B01J31/06 ,  B01J31/125 ,  B01J31/127 ,  B01J35/1019 ,  B01J35/1023 ,  B01J35/1028 ,  B01J35/1038 ,  B01J35/1042 ,  B01J35/1047 ,  B01J35/1057 ,  B01J35/1061 ,  B01J37/036 ,  B01J2220/86 ,  B01J2231/641 ,  B01J2231/646 ,  C01B37/00 ,  C01B39/00 ,  C07F7/00 ,  C08F2/00 ,  C08F2/10 ,  C08F2/42 ,  C08F4/65916 ,  C08F4/65925 ,  C08G77/50 ,  C08G77/58 ,  C08G77/60 ,  C10G25/003 ,  C10G35/06 ,  C10G45/00 ,  C10G45/04 ,  C10G45/34 ,  C10G45/44 ,  C10G45/46 ,  C10G45/60 ,  C10G45/64 ,  C10G47/02 ,  C10G50/00 ,  C10K1/32 ,  C10M101/02 ,  C10M177/00 ,  C10M2203/1006 ,  C10N2270/00

Abstract: A catalyst system comprising a combination of: 1) one or more catalyst compounds comprising at least one nitrogen linkage; 2) a support comprising an organosilica material, which is a mesoporous organosilica material; and 3) an optional activator. Useful catalysts include pyridyldiamido transition metal complexes, HN5 compounds, and bis(imino)pyridyl complexes. The organosilica material is a polymer of at least one monomer of Formula [Z1OZ2SiCH2]3(1), where Z1 represents a hydrogen atom, a C1-C4alkyl group, or a bond to a silicon atom of another monomer and Z2 represents a hydroxyl group, a C1-C4alkoxy group, a C1-C6 alkyl group, or an oxygen atom bonded to a silicon atom of another monomer. This invention further relates to processes to polymerize olefins comprising contacting one or more olefins with the above catalyst system.

公开(公告)号：US20170313791A1

公开(公告)日：2017-11-02

申请号：US15526521

申请日：2015-12-11

Applicant: ExxonMobil Research and Engineering Company

Inventor： Machteld M.W. MERTENS ,  Jo Ann M. CANICH ,  Suzzy C.H. HO ,  Quanchang LI

IPC: C08F6/00 ,  B01D15/08

CPC classification number: C08F6/005 ,  B01D15/00 ,  B01D15/08 ,  B01D53/02 ,  B01D53/0462 ,  B01D53/047 ,  B01D67/0088 ,  B01D69/10 ,  B01D71/70 ,  B01D2253/108 ,  B01D2253/20 ,  B01D2253/25 ,  B01D2256/245 ,  B01D2257/302 ,  B01D2257/304 ,  B01D2257/40 ,  B01D2257/504 ,  B01D2257/7022 ,  B01D2257/80 ,  B01J20/0229 ,  B01J20/0237 ,  B01J20/06 ,  B01J20/08 ,  B01J20/10 ,  B01J20/103 ,  B01J20/16 ,  B01J20/18 ,  B01J20/22 ,  B01J20/262 ,  B01J20/264 ,  B01J20/28011 ,  B01J20/28057 ,  B01J20/28061 ,  B01J20/28064 ,  B01J20/28066 ,  B01J20/28069 ,  B01J20/28071 ,  B01J20/28073 ,  B01J20/28076 ,  B01J20/28078 ,  B01J20/28083 ,  B01J20/286 ,  B01J20/3042 ,  B01J20/3204 ,  B01J20/3212 ,  B01J20/3236 ,  B01J20/3238 ,  B01J20/3272 ,  B01J23/44 ,  B01J31/0274 ,  B01J35/1019 ,  B01J35/1023 ,  B01J35/1028 ,  B01J35/1061 ,  B01J37/0213 ,  B01J37/0236 ,  B01J2220/86 ,  C01B37/00 ,  C07F7/0807 ,  C07F7/081 ,  C07F7/0818 ,  C08G77/26 ,  C08G77/60 ,  C10G25/003 ,  C10G31/09 ,  C10G35/06 ,  C10G45/00 ,  C10G45/04 ,  C10G45/34 ,  C10G45/44 ,  C10G45/46 ,  C10G45/52 ,  C10G45/60 ,  C10G45/64 ,  C10G47/02 ,  C10G50/00 ,  C10G57/02 ,  C10K1/32 ,  C10M101/02 ,  C23C16/56 ,  Y02P20/582

Abstract: A photovoltaic module comprising: (a) a photovoltaic laminate including: two or more electrically conducting dements extending through the photovoltaic laminate so that power is moved from one photovoltaic module towards another photovoltaic module or towards an inverter; and (b) one or more connectors connected to each of the two or more electrically conducting elements by a connection joint, each of the one or more connectors include: two or more opposing terminals that each are connected to and extend from one of the two or more electrically conducing elements; wherein a dielectric space is located between the two or more opposing terminals and the dielectric space blocks material used to form a connection joint from passing from a first terminal to a second terminal, the material from the connection joint cools before the material passes from one terminal to a second terminal, the material fails to travel from the first terminal to the second terminal, or a combination thereof.

公开(公告)号：US09733234B2

公开(公告)日：2017-08-15

申请号：US14738688

申请日：2015-06-12

Applicant: Janusz B. Pawliszyn ,  German Augusto Gomez Rios

Inventor： Janusz B. Pawliszyn ,  German Augusto Gomez Rios

IPC: G01N1/02 ,  G01N30/00 ,  H01J49/16 ,  G01N33/50 ,  G01N1/40 ,  H01J49/04 ,  B01J20/286 ,  A61B10/00 ,  G01N30/06 ,  G01N30/72

CPC classification number: G01N33/50 ,  A61B10/0045 ,  B01J20/286 ,  G01N1/405 ,  G01N30/7233 ,  G01N2030/009 ,  G01N2030/062 ,  G01N2560/00 ,  H01J49/0409 ,  H01J49/168 ,  Y10T428/2933

Abstract: A device is described for generating ionized molecules for analysis in a mass spectrometer. The device includes: a solid substrate having one or more edges and a coated area that is coated with an extraction phase comprising an extraction polymer. The solid substrate may have at least two edges that meet at an angle from about 8° to about 180°. Mass spectrometry systems that include such a device are also described. Methods of analyzing a molecule previously extracted from a sample onto the device are also described.

公开(公告)号：US20170226153A1

公开(公告)日：2017-08-10

申请号：US15501020

申请日：2015-07-27

Applicant: Univerzita Pardubice

Inventor： Rudolf Kupcik ,  Zuzana Bilkova ,  Pavel Rehulka ,  Jan Macak

IPC: C07K1/22 ,  C07H21/04 ,  B01D15/42 ,  B01J20/28 ,  B01D15/38 ,  C07H1/06 ,  B01J20/06

CPC classification number: C07K1/22 ,  B01D15/3804 ,  B01D15/3828 ,  B01D15/388 ,  B01D15/3885 ,  B01D15/422 ,  B01D2239/0258 ,  B01J20/06 ,  B01J20/103 ,  B01J20/28004 ,  B01J20/28009 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/3236 ,  B01J20/3293 ,  C07H1/06 ,  C07H21/04 ,  C07K2319/21 ,  G01N2030/567 ,  H01F1/0054

Abstract: The invention relates to a method for separation of biopolymer molecules, particularly biopolymer molecules from the group consisting of mono- a multi-phosphorylated peptides, recombinant peptides/proteins with a polyhistidine tag (His-tag) or with another chemically similar biospecific tag, cysteine-containing peptides/proteins and nucleic acids, in which a biopolymer molecule is bound in a binding solution by a specific binding to a carrier, which contains a core with dimensions in nano- and/or submicro- and/or microscale, which is composed of oxide of at least one transition metal and/or silicon oxide, on whose surface is deposited at least one continuous or non-continuous layer and/or nanoparticles of magnetic metal oxide and/or such nanoparticles are deposited in its inner structure, and subsequently undesirable and non-specifically bound components are washed off at least once from the carrier-bound bio-molecules by a washing solution, whereupon biopolymer molecules are eluted from it by changing pH and/or by using an elution solution. The invention also relates to a carrier for application of this method.

公开(公告)号：US20170210871A1

公开(公告)日：2017-07-27

申请号：US15327940

申请日：2015-07-22

Applicant: DAICEL CORPORATION

Inventor： Toru SHIBATA ,  Yasuto MORISHITA ,  Yosuke ITO

IPC: C08J9/26 ,  C07K1/22 ,  B01D15/38 ,  B01J20/24 ,  B01J20/28 ,  C07K16/00 ,  C08J3/075

CPC classification number: C08J9/26 ,  B01D15/3809 ,  B01J20/24 ,  B01J20/28019 ,  B01J20/28042 ,  B01J20/285 ,  B01J20/286 ,  B01J20/3007 ,  B01J20/3078 ,  B01J20/3212 ,  B01J20/3274 ,  C07K1/22 ,  C07K16/00 ,  C08J3/075 ,  C08J3/16 ,  C08J2301/12

Abstract: The present invention provides a technique for preparing a porous cellulose medium, through the gelation of a solution in which cellulose acetate as a raw material is dissolved, which does not cause a reaction involving the migration of substances. Specifically, a method for producing a porous cellulose medium is provided which includes the steps of gelling a flowable homogeneous composition containing cellulose acetate, an organic solvent, and water through a decrease in temperature; and hydrolyzing the cellulose acetate contained in the resulting gel.

公开(公告)号：US09676816B2

公开(公告)日：2017-06-13

申请号：US14378230

申请日：2014-01-21

Applicant: THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSAS

Inventor： Suresh Kumar Thallapuranam ,  Srinivas Jayanthi ,  Jacqueline Morris ,  Alicia Brown ,  David McNabb ,  Ralph Henry

IPC: C12N9/64 ,  C07K14/00 ,  C07K1/22 ,  B01D15/20 ,  B01D15/22 ,  B01D15/38 ,  B01D69/08 ,  B01J20/28 ,  B01J20/286

CPC classification number: C07K1/22 ,  B01D15/206 ,  B01D15/22 ,  B01D15/3809 ,  B01D69/08 ,  B01J20/28078 ,  B01J20/286 ,  C07K14/00 ,  C07K2319/20 ,  C07K2319/50 ,  C12N9/6424 ,  C12Y304/21043

Abstract: In one aspect, affinity tags for recombinant protein purification are described herein which, in some embodiments, can mitigate or overcome disadvantages of prior affinity tag systems. In some embodiments, for example, affinity tags described herein permit efficient elution of desired recombinant proteins with simplified solution systems, such as alkali metal salt solutions. An affinity tag described herein comprises an amino acid sequence including a repeating amino acid unit of BXXXBXX, wherein B is an amino acid selected from the group consisting of histidine, lysine and arginine and X is an amino acid selected from the group consisting of amino acids other than histidine, lysine and arginine.

公开(公告)号：US09663559B2

公开(公告)日：2017-05-30

申请号：US15063471

申请日：2016-03-07

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Martin Hall ,  Sture Larsson ,  Andreas Muranyi ,  Gustav Rodrigo ,  Jinyu Zou ,  Per-Mikael Aberg

IPC: C07K14/31 ,  B01D15/38 ,  B01J20/286 ,  C07K1/22 ,  B01J20/32 ,  C07K16/00 ,  C07K17/00

CPC classification number: B01J20/24 ,  B01D15/3809 ,  B01J20/28019 ,  B01J20/285 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3274 ,  B01J20/3293 ,  B01J2220/52 ,  B01J2220/54 ,  C07K1/22 ,  C07K14/31 ,  C07K16/00 ,  C07K17/00 ,  C07K17/10 ,  C07K2317/55

Abstract: The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.