公开(公告)号：US20100019146A1

公开(公告)日：2010-01-28

申请号：US12501011

申请日：2009-07-10

Applicant: Hidetoshi Nishiyama ,  Mitsuru Koizumi ,  Mitsuo Suga

Inventor： Hidetoshi Nishiyama ,  Mitsuru Koizumi ,  Mitsuo Suga

IPC: G01N23/225

CPC classification number: H01J37/20 ,  H01J37/28 ,  H01J2237/2004

Abstract: Specimen holder, specimen inspection apparatus, and specimen inspection method for observing or inspecting a specimen consisting of cultured cells. The specimen holder has a body portion and a film. The body portion has a specimen-holding surface opened to permit access from the outside. The film has a first surface forming the specimen-holding surface. The specimen disposed on the first surface of the film can be irradiated with a primary beam for observation or inspection of the specimen via the film. A region coated with an electrically conductive film is formed on the bottom surface of the body portion facing away from the specimen-holding surface. An optically transparent region not coated with the electrically conductive film is also formed on the bottom surface.

Abstract translation: 样本支架，样本检查装置和用于观察或检查由培养细胞组成的标本的样本检查方法。 试样架具有主体部分和薄膜。 主体部分具有打开以允许从外部进入的标本保持表面。 膜具有形成试样保持面的第一表面。 可以利用主光束照射设置在膜的第一表面上的试样，以通过膜观察或检查试样。 涂覆有导电膜的区域形成在主体部分的背离标本保持表面的底表面上。 未涂覆导电膜的光学透明区域也形成在底面上。

公开(公告)号：US20070145289A1

公开(公告)日：2007-06-28

申请号：US11584559

申请日：2006-10-23

Applicant: Chih-Yu Chao ,  Wen-Jiunn Hsieh

Inventor： Chih-Yu Chao ,  Wen-Jiunn Hsieh

IPC: G21K5/10

CPC classification number: H01J37/20 ,  H01J2237/188 ,  H01J2237/2004 ,  H01J2237/262

Abstract: A closed observational device for an electron microscope is formed of a housing. The housing includes a liquid chamber formed therein, at least one view hole formed at each of a top side thereof and a bottom side thereof and communicating with the liquid chamber and coaxially aligned with the other, and a film mounted to and sealing each of the view holes. Accordingly, a general specimen or a live cell can be placed into the liquid chamber for microscopic observation under the electron microscope. Besides, the present invention can enclose the liquid inside the housing to prevent the liquid from exhausting outward or volatilization.

Abstract translation: 用于电子显微镜的闭合观察装置由壳体形成。 壳体包括形成在其中的液体室，形成在其顶侧和底侧的至少一个视野孔，并且与液体室连通并与另一个同轴地对准，并且安装并密封每个 查看孔。 因此，可以在电子显微镜下将一般试样或活细胞置于液室内进行显微镜观察。 此外，本发明可以将液体包围在壳体内，以防止液体向外或挥发中排出。

公开(公告)号：US09857320B2

公开(公告)日：2018-01-02

申请号：US15314141

申请日：2015-06-18

Applicant: LEIBNIZ-INSTITUT FUER NEUE MATERIALIEN GEMEINNUETZIGE GMBH

Inventor： Niels De Jonge ,  Diana B. Peckys

IPC: G01N23/00 ,  G01N23/225 ,  H01J37/20

CPC classification number: G01N23/2251 ,  G01N2223/307 ,  G01N2223/418 ,  G01N2223/612 ,  H01J37/20 ,  H01J37/228 ,  H01J2237/2004 ,  H01J2237/24455 ,  H01J2237/2802

Abstract: The present invention relates to a device and a method for the stoichiometric analysis of samples.In order to study the spatial distribution of different proteins in the plasma membrane of a complete cell within a short time frame, a device and a method are proposed for the stoichiometric analysis of samples.The object is established by means of a device for the stoichiometric analysis of samples, said device comprising a) a sample processing device comprising a sample holder for holding the sample, means for setting the temperature, means for adding and removing fluids (including gases) and at least one fluid reservoir, b) an electron microscope with a detector, and c) a computer-controlled process control system for controlling the means for setting the temperature and the means for adding and removing fluids (including gases), a computer-controlled and automated imaging device that captures images by means of the electron microscope, a unit that stores the captured images and an image analysis unit controlled by the computer.

公开(公告)号：US09842722B2

公开(公告)日：2017-12-12

申请号：US14964586

申请日：2015-12-10

Applicant: Industrial Technology Research Institute

Inventor： Hsin-Hung Lee ,  Cheng-Yu Lee ,  Chun-Lin Chiang ,  Kun-Chih Tsai ,  Win-Ti Lin

IPC: H01J37/18 ,  H01J37/20 ,  H01J37/28 ,  G01N35/10

CPC classification number: H01J37/20 ,  G01N35/1095 ,  H01J37/28 ,  H01J2237/18 ,  H01J2237/2004 ,  H01J2237/2065

Abstract: An electronic microscope includes a carrier, a first driving unit, a flow-buffer unit and an electron source. The carrier carries a sample. The first driving unit drives a first fluid to flow along a first flow path, wherein the first flow path passes through the sample. The flow-buffer unit is disposed on the first flow path to perform buffering on the first fluid, wherein the first fluid flows through the flow-buffer unit and the carrier in sequence. The electron source provides an electron beam to the sample.

公开(公告)号：US20170062174A1

公开(公告)日：2017-03-02

申请号：US15252403

申请日：2016-08-31

Applicant: Hitachi High-Tech Science Corporation

Inventor： Toshiyuki Iwahori ,  Masakatsu Hasuda

IPC: H01J37/18 ,  H01J37/285 ,  H01J37/20

CPC classification number: H01J37/185 ,  H01J37/20 ,  H01J37/285 ,  H01J2237/2002 ,  H01J2237/2004 ,  H01J2237/2006 ,  H01J2237/31749

Abstract: Provided is a transport device in which a reduction in the size and a reduction in the cost of the entire device are possible. A transport unit which is interposed between a sub-chamber and a first treatment chamber is provided with: a transport bar which transports a sample along a right-left direction in a preliminary sample chamber; a support which supports the transport bar; a case which supports the support so as to be rotatable around a rotation axis intersecting the right-left direction; and a second O-ring which seals an inside of the case. The preliminary sample chamber and a first treatment space can communicate with each other through the inside of the case. The transport bar is made so as to be able to transition between a first state where the transport bar can transport the sample between the preliminary sample chamber and the inside of the case, and a second state where the transport bar can transport the sample between the first treatment space and the inside of the case, by rotation of the support with respect to the case.

Abstract translation: 提供了可以减小整体装置的尺寸和降低成本的运送装置。 插入在副室和第一处理室之间的输送单元设置有：在预备样品室中沿左右方向输送样品的输送条; 支持运输栏; 支撑支撑件以能够围绕与左右方向相交的旋转轴线旋转的壳体; 以及密封壳体内部的第二O形环。 预备样品室和第一处理空间可以通过壳体内部彼此连通。 输送条被制成能够在输送条可以在预备样品室和壳体内部之间输送样品的第一状态和第二状态之间转移，其中输送条可以将样品在 第一处理空间和壳体的内部，通过支撑件相对于壳体的旋转。

公开(公告)号：US09449787B2

公开(公告)日：2016-09-20

申请号：US14817551

申请日：2015-08-04

Applicant: Vivekananda P. Adiga ,  Gabriel Dunn ,  Alexander K. Zettl ,  A. Paul Alivisatos

Inventor： Vivekananda P. Adiga ,  Gabriel Dunn ,  Alexander K. Zettl ,  A. Paul Alivisatos

IPC: G02B1/12 ,  H01J37/16 ,  H01J37/26 ,  H01J37/20 ,  H01L29/68 ,  H01L29/78 ,  H01L29/16 ,  C23C18/08 ,  H01L29/66 ,  G02B21/16

CPC classification number: H01J37/20 ,  C23C18/08 ,  G02B1/12 ,  G02B21/16 ,  H01J37/26 ,  H01J2237/2003 ,  H01J2237/2004 ,  H01L29/1606 ,  H01L29/66045 ,  Y10T29/49002

Abstract: This disclosure provides systems, methods, and apparatus related to liquid flow cells for microscopy. In one aspect, a device includes a substrate having a first and a second oxide layer disposed on surfaces of the substrate. A first and a second nitride layer are disposed on the first and second oxide layers, respectively. A cavity is defined in the first oxide layer, the first nitride layer, and the substrate, with the cavity including a third nitride layer disposed on walls of the substrate and the second oxide layer that define the cavity. A channel is defined in the second oxide layer. An inlet port and an outlet port are defined in the second nitride layer and in fluid communication with the channel. A plurality of viewports is defined in the second nitride layer. A first graphene sheet is disposed on the second nitride layer covering the plurality of viewports.

Abstract translation: 本公开提供了与用于显微镜的液体流动池相关的系统，方法和装置。 一方面，一种器件包括具有设置在衬底表面上的第一和第二氧化物层的衬底。 第一和第二氮化物层分别设置在第一和第二氧化物层上。 在第一氧化物层，第一氮化物层和衬底中限定空腔，空腔包括设置在衬底的壁上的第三氮化物层和限定空腔的第二氧化物层。 在第二氧化物层中限定通道。 入口端口和出口限定在第二氮化物层中并与通道流体连通。 在第二氮化物层中限定多个视口。 第一石墨烯片被布置在覆盖多个视口的第二氮化物层上。

公开(公告)号：US09162211B2

公开(公告)日：2015-10-20

申请号：US12912468

申请日：2010-10-26

Applicant: Gerard Anne Nicolaas Van Veen ,  Jacobus Peter Johannes Peters ,  Pleun Dona ,  Alan Frank De Jong

Inventor： Gerard Anne Nicolaas Van Veen ,  Jacobus Peter Johannes Peters ,  Pleun Dona ,  Alan Frank De Jong

IPC: G01N15/14 ,  G01N21/05 ,  B01J19/00 ,  B01L3/00 ,  H01J37/20 ,  H01J37/26 ,  B01L7/00

CPC classification number: B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00824 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/0086 ,  B01J2219/00905 ,  B01J2219/0097 ,  B01L3/502761 ,  B01L7/54 ,  B01L2200/0668 ,  B01L2300/0822 ,  B01L2400/0415 ,  B01L2400/0439 ,  B01L2400/0442 ,  B01L2400/0487 ,  G01N2021/058 ,  H01J37/20 ,  H01J37/26 ,  H01J2237/2004 ,  Y10T436/143333

Abstract: A micro-reactor is provided for observing small particles, cells, bacteria, viruses or protein molecules in a fluid. The micro-reactor includes a first channel for containing the fluid and a second channel adjacent to the first channel. A gap connects the first channel and the second channel and a window transparent to the method of inspection is provided at the gap. A static or dynamic gradient, such as a gradient in concentration of a chemical or biological material, in pressure, in temperature, in electric potential, or in magnetic field, is applied across the gap, thereby causing the particles to cross the gap. By detecting a property of the particles upstream in the first channel and then applying a pressure burst over the channels when the property meets certain pre-set criteria, only selected particles can be placed in the gap.

Abstract translation: 提供了用于在流体中观察小颗粒，细胞，细菌，病毒或蛋白质分子的微反应器。 微反应器包括用于容纳流体的第一通道和与第一通道相邻的第二通道。 间隙连接第一通道和第二通道，并且在间隙处提供对检查方法透明的窗口。 在间隙中施加静态或动态梯度，例如化学或生物材料在压力，温度，电位或磁场中的浓度梯度，从而使颗粒穿过间隙。 通过检测第一通道上游的颗粒的性质，然后当性能达到某些预设标准时，在通道上施加压力突发，只有选定的颗粒才能放置在间隙中。

公开(公告)号：US08852408B2

公开(公告)日：2014-10-07

申请号：US12432037

申请日：2009-04-29

Applicant: S. Jay Chey ,  Mark den Heijer ,  Aparna Prabhakar ,  Frances M. Ross ,  Ranjani Sirdeshmukh

Inventor： S. Jay Chey ,  Mark den Heijer ,  Aparna Prabhakar ,  Frances M. Ross ,  Ranjani Sirdeshmukh

IPC: C25B9/00 ,  C25B9/06 ,  C25B9/12 ,  C25B1/04 ,  G01N27/416

CPC classification number: G01N27/416 ,  H01J37/20 ,  H01J2237/2004 ,  H01J2237/206 ,  H01J2237/26

Abstract: An electrochemical cell apparatus is disclosed where the cell has a chamber for containing an electrolyte. The chamber is situated between a bottom and a top substrate. One or more bottom windows are in the bottom substrate and one or more top windows are in the top substrate. Each window has a window cover facing the chamber. The top window and bottom window each have a portion in alignment so that an electron beam passes through both respective portions. A spacer is deposited between the top and bottom substrate and forming walls surrounding the chamber. Two or more electrodes, each having an interior portion that is within the chamber and electrically continuous with an exterior portion external to the chamber, are located on the chamber side of the bottom substrate.

Abstract translation: 公开了一种电化学电池装置，其中电池具有用于容纳电解质的室。 腔室位于底部和顶部衬底之间。 一个或多个底部窗口位于底部基板中，并且一个或多个顶部窗口位于顶部基板中。 每个窗口都有一个朝向腔室的窗口盖。 顶窗和底窗各自具有对准的部分，使得电子束通过两个相应的部分。 间隔件沉积在顶部和底部基底之间以及形成围绕该腔室的壁。 两个或更多个电极，每个具有位于室内部的内部部分并且与室外部的外部部分电连接，位于底部基板的腔室侧。

公开(公告)号：US20140227734A1

公开(公告)日：2014-08-14

申请号：US14343537

申请日：2012-09-07

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Takahiko Hariyama ,  Yasuharu Takaku ,  Hiroshi Suzuki ,  Yoshinori Muranaka ,  Isao Ohta ,  Masatsugu Shimomura ,  Daisuke Ishii

IPC: G01N1/44 ,  G01N23/225

CPC classification number: G01N1/44 ,  G01N1/2853 ,  G01N23/04 ,  G01N23/225 ,  G01N23/2252 ,  G01N2223/612 ,  H01J37/20 ,  H01J2237/2002 ,  H01J2237/2004

Abstract: Provided is an observation method by an electron microscope, in which a biological sample can be observed as it is alive and a situation that the biological sample is moving can be observed using an electron microscope, and a composition for evaporation suppression under vacuum, a scanning electron microscope, and a transmission electron microscope used in the method.The sample observation method by an electron microscope according to the invention includes applying a composition for evaporation suppression containing at least one kind selected from an amphiphilic compound, oils and fats, and an ionic liquid to the surface of a sample to form a thin film, and covering the sample with the thin film, and displaying an electron microscopic image of the sample, which is covered with the thin film and accommodated in a sample chamber under vacuum, on a display device.

Abstract translation: 提供一种电子显微镜的观察方法，其中可以观察生物样品的活性，并且可以使用电子显微镜观察生物样品移动的情况，以及用于真空下蒸发抑制的组合物，扫描 电子显微镜和用于该方法的透射电子显微镜。 通过根据本发明的电子显微镜的样品观察方法包括将包含选自两亲性化合物，油和脂肪中的至少一种的蒸发抑制组合物和离子液体施加到样品的表面以形成薄膜， 并用薄膜覆盖样品，并在显示装置上显示被薄膜覆盖并在真空下容纳在样品室中的样品的电子显微镜图像。

公开(公告)号：US20110303845A1

公开(公告)日：2011-12-15

申请号：US13201820

申请日：2010-01-20

Applicant: Toshie Yaguchi ,  Yasuhira Nagakubo ,  Takeo Kamino ,  Akira Watabe

Inventor： Toshie Yaguchi ,  Yasuhira Nagakubo ,  Takeo Kamino ,  Akira Watabe

IPC: H01J37/26

CPC classification number: H01J37/20 ,  H01J2237/006 ,  H01J2237/2002 ,  H01J2237/2003 ,  H01J2237/2004

Abstract: An object of the invention is to provide an electron beam device and a sample holding device for the electron beam device that can observe the reaction between a sample and a gas at high resolution while a gas atmosphere is maintained even by using thin diaphragms.To solve one of the problems described above, in an electron beam device having the function of separately exhausting an electron beam irradiation portion of an optical column, a sample chamber and an observation chamber, a gas supply means for supplying a gas to a sample and an exhaust means for exhausting a gas are provided to sample holding means, diaphragms are disposed above and below the sample to separate the gas atmosphere and vacuum of the sample chamber and to constitute a cell sealing the atmosphere around the sample, and a mechanism for spraying a gas is provided to the outside of the diaphragms. The gas sprayed outside the diaphragms has low electron beam scattering performance such as hydrogen, oxygen or nitrogen. The diaphragm is an amorphous film formed of a light element such as a carbon film, an oxide film and a nitride film capable of transmitting the electron beam.

Abstract translation: 本发明的目的是提供一种用于电子束装置的电子束装置和样品保持装置，其可以在通过使用薄隔膜保持气体气氛的同时以高分辨率观察样品和气体之间的反应。 为了解决上述问题之一，在具有分别排出光学柱的电子束照射部分，样品室和观察室的功能的电子束装置中，向样品供应气体的气体供给装置和 向样品保持装置提供用于排出气体的排气装置，在样品上方和下方设置隔膜以分离样品室的气体气氛和真空，并构成密封样品周围的气氛的单元，以及用于喷射的机构 气体被提供到隔膜的外部。 喷射在隔膜外部的气体具有低电子束散射性能，例如氢，氧或氮。 隔膜是由诸如碳膜，氧化膜和能够透射电子束的氮化物膜的光元件形成的非晶膜。