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公开(公告)号：US09810618B2
公开(公告)日：2017-11-07
申请号：US14808211
申请日：2015-07-24
Applicant: Abbott Laboratories
Inventor： Jiong Wu , Michael R. Buhl , Giacomo Vacca
CPC classification number: G01N15/1434 , G01N15/1459 , G01N21/6428 , G01N33/49 , G01N2015/0069 , G01N2015/008 , G01N2015/1006 , G01N2015/1402 , G01N2015/1477 , G01N2015/1486 , G01N2015/1488 , G01N2021/6439
Abstract: Provided herein are systems and methods for analyzing blood samples, and more specifically for performing a basophil analysis. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; and then (b) using measurements of light scatter and fluorescence emission to distinguish basophils from other WBC sub-populations. In one embodiment, the systems and methods include performing a basophil cluster analysis of the blood sample, based on the combination of light scatter and fluorescence measurements.
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公开(公告)号：US20170276591A1
公开(公告)日：2017-09-28
申请号：US15581395
申请日：2017-04-28
Applicant: Abbott Laboratories
Inventor： Martin Krockenberger , Jiong Wu , Bodo Roemer , Giacomo Vacca
CPC classification number: G01N15/1404 , G01N15/00 , G01N15/1429 , G01N15/1459 , G01N21/53 , G01N33/49 , G01N33/4915 , G01N33/492 , G01N2015/0073 , G01N2015/0076 , G01N2015/008 , G01N2015/0084 , G01N2015/1006 , G01N2015/1486 , G01N2800/22 , Y10T436/101666
Abstract: A method for identifying, analyzing, and quantifying the cellular components of whole blood by means of an automated hematology analyzer and the detection of the light scattered, absorbed, and fluorescently emitted by each cell. More particularly, the aforementioned method involves identifying, analyzing, and quantifying the cellular components of whole blood by means of a light source having a wavelength ranging from about 400 nm to about 450 nm and multiple in-flow optical measurements and staining without the need for lysing red blood cells.
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公开(公告)号：US20170176481A1
公开(公告)日：2017-06-22
申请号：US15378479
申请日：2016-12-14
Applicant: Abbott Laboratories
Inventor： Roger W. Accurso , Jiong Wu , Richard G. Kendall , James A. Janik , Ronald J. Shanafelter , Roger Tabey , Svitlana Y. Berezhna , Trevor D. Shields , Mahmoud Janbakhsh , Daryl Wong , Jorge Haro , Michael J. Kennedy
CPC classification number: G01N35/021 , G01D5/342 , G01N1/312 , G01N21/13 , G01N35/00029 , G01N35/00732 , G01N35/00871 , G01N35/0099 , G01N35/04 , G01N2035/00138 , G01N2035/00326 , G01N2035/00801 , G01N2035/0412 , G01N2035/0493 , G01N2201/0438
Abstract: Aspects of the present disclosure include systems and methods. According to certain embodiments, provided is an integrated analysis system that includes a first module including a sample analysis component and a first internal container conveyor system. The integrated analysis system further includes a second module including a second internal container conveyor system. The first and second modules are positioned adjacent each other such that the first and second internal container conveyor systems are aligned and adapted to transport containers from the first module to the second module. Also provided are methods of analyzing and preparing samples (e.g., blood and body fluid samples), as well as components that find use within the analysis systems of the present disclosure.
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公开(公告)号：US20160018313A1
公开(公告)日：2016-01-21
申请号：US14808725
申请日：2015-07-24
Applicant: Abbott Laboratories
Inventor： Jiong Wu , Giacomo Vacca
CPC classification number: G01N15/1434 , G01N15/147 , G01N21/6428 , G01N33/5047 , G01N33/5094 , G01N33/582 , G01N35/1002 , G01N2015/0065 , G01N2015/0069 , G01N2015/1006 , G01N2015/1486 , G01N2015/149 , G01N2021/6439
Abstract: A method whereby one or more fluorescent dyes are used to bind and stain nucleic acids in certain blood cells, such as, for example, white blood cells, nucleated red blood cells, and reticulocytes, and to induce fluorescent emissions upon excitation of photons from a given source of light, such as, for example, a laser, at an appropriate wavelength. More particularly, this invention provides a method whereby a fluorescent trigger is used in a data collection step for collecting events that emit strong fluorescence, in order to separate white blood cells and nucleated red blood cells from red blood cells and platelets without the need for using a lysing agent.
Abstract translation: 一种或多种荧光染料用于结合和染色某些血细胞（例如白细胞，有核红细胞和网织红细胞）中的核酸的方法，以及在从 给定光源，例如激光，以适当的波长。 更具体地，本发明提供了一种方法，其中在用于收集发射强荧光的事件的数据收集步骤中使用荧光触发器，以便将白细胞和有核红细胞与红细胞和血小板分离而不需要使用 裂解剂。
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公开(公告)号：US20160018310A1
公开(公告)日：2016-01-21
申请号：US14808205
申请日：2015-07-24
Applicant: Abbott Laboratories
Inventor： Jiong Wu , Giacomo Vacca
CPC classification number: G01N15/1434 , G01N15/1459 , G01N21/6428 , G01N33/49 , G01N2015/008 , G01N2015/1006 , G01N2015/1477 , G01N2015/1488 , G01N2021/6439
Abstract: Systems and methods for analyzing blood samples, and more specifically for performing a white blood cell (WBC) differential analysis. The systems and methods screen WBCs by means of fluorescence staining and a fluorescence triggering strategy. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder WBC reagent(s), suitable for assays of samples containing fragile WBCs. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye, which corresponds in emission spectrum to an excitation source of a hematology instrument; (b) using a fluorescence trigger to screen the blood sample for WB Cs; and (c) using measurements of (1) axial light loss, (2) intermediate angle scatter, (3) 90° polarized side scatter, (4) 90° depolarized side scatter, and (5) fluorescence emission to perform a differentiation analysis.
Abstract translation: 用于分析血液样品的系统和方法，更具体来说用于进行白细胞（WBC）差异分析。 系统和方法通过荧光染色和荧光触发策略筛选WBC。 因此，基本上消除了来自未分解的红细胞（RBC）和裂解的RBC的片段的干扰。 这些系统和方法还可以开发出相对温和的WBC试剂，适用于含有脆弱WBC的样品的测定。 在一个实施方案中，系统和方法包括：（a）用发射光谱对应于血液学仪器的激发源的专用细胞膜可渗透荧光染料染色血液样品; （b）使用荧光触发器筛选WB Cs的血样; 和（c）使用（1）轴向光损失，（2）中角散射，（3）90°偏振侧散射，（4）90°去极化侧散射的测量，和（5）荧光发射来进行微分分析 。
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Patent Agency Ranking
公开(公告)号：US11193927B2
公开(公告)日：2021-12-07
申请号：US16330289
申请日：2017-09-07
Applicant: Abbott Laboratories
Inventor： Wenjing Li , Emily H. Lin , Jiong Wu
Abstract: Methods, devices, and systems for automated cellular analysis of a body fluid sample are disclosed. The methods, devices, and systems apply watershed transform to data, generated by flowing a body fluid sample through a flow cytometer, to determine threshold(s) to be used for analysis of the data.
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公开(公告)号：US10774359B2
公开(公告)日：2020-09-15
申请号：US15979230
申请日：2018-05-14
Applicant: Abbott Laboratories
Inventor： Jiong Wu , Emily H. Lin , Jihping Yang
Abstract: Herein is provided a simple, reliable and accurate method for cellular analysis on hematology analyzers. In various aspects, the methods provide separation and/or differentiation between red blood cells (RBCs) and white blood cells (WBCs) by utilizing a fluorescent dye to selectively stain WBCs such that they emit stronger fluorescence signals. The method provides optimal detection limits on WBCs and RBCs, thereby allowing analysis of samples with sparse cellular concentrations. As few as one reagent may be used to prepare a single dilution for body fluid analysis, in order to simplify the body fluid analysis. Minimal damage to WBCs is attained using the lysis-free approach described in aspects of the disclosure.
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公开(公告)号：US10648995B2
公开(公告)日：2020-05-12
申请号：US15378479
申请日：2016-12-14
Applicant: Abbott Laboratories
Inventor： Roger W. Accurso , Jiong Wu , Richard G. Kendall , James A. Janik , Ronald J. Shanafelter , Robert Fahey , Svitlana Y. Berezhna , Trevor D. Shields , Mahmoud Janbakhsh , Daryl Wong , Jorge Haro , Michael J. Kennedy
Abstract: Aspects of the present disclosure include systems and methods. According to certain embodiments, provided is an integrated analysis system that includes a first module including a sample analysis component and a first internal container conveyor system. The integrated analysis system further includes a second module including a second internal container conveyor system. The first and second modules are positioned adjacent each other such that the first and second internal container conveyor systems are aligned and adapted to transport containers from the first module to the second module. Also provided are methods of analyzing and preparing samples (e.g., blood and body fluid samples), as well as components that find use within the analysis systems of the present disclosure.
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公开(公告)号：US10481071B2
公开(公告)日：2019-11-19
申请号：US15704972
申请日：2017-09-14
Applicant: Abbott Laboratories
Inventor： Jiong Wu , Giacomo Vacca
Abstract: Systems and methods for analyzing blood samples, and more specifically for performing a white blood cell (WBC) differential analysis. The systems and methods screen WBCs by means of fluorescence staining and a fluorescence triggering strategy. As such, interference from unlyzed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder WBC reagent(s), suitable for assays of samples containing fragile WBCs. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye, which corresponds in emission spectrum to an excitation source of a hematology instrument; (b) using a fluorescence trigger to screen the blood sample for WBCs; and (c) using measurements of (1) axial light loss, (2) intermediate angle scatter, (3) 90° polarized side scatter, (4) 90° depolarized side scatter, and (5) fluorescence emission to perform a differentiation analysis.
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公开(公告)号：US20190204301A1
公开(公告)日：2019-07-04
申请号：US16330289
申请日：2017-09-07
Applicant: Abbott Laboratories
Inventor： Wenjing Li , Emily H. Lin , Jiong Wu
CPC classification number: G01N33/5094 , G01N33/5002 , G01N33/52
Abstract: Methods, devices, and systems for automated cellular analysis of a body fluid sample are disclosed. The methods, devices, and systems apply watershed transform to data, generated by flowing a body fluid sample through a flow cytometer, to determine threshold(s) to be used for analysis of the data.
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