公开(公告)号：US07928380B2

公开(公告)日：2011-04-19

申请号：US12342897

申请日：2008-12-23

Applicant: Mitsuo Suga ,  Hidetoshi Nishiyama

Inventor： Mitsuo Suga ,  Hidetoshi Nishiyama

IPC: G01N23/02

CPC classification number: H01J37/20 ,  H01J37/26 ,  H01J2237/2004 ,  H01J2237/2005 ,  H01J2237/201 ,  H01J2237/204 ,  H01J2237/206 ,  H01J2237/262 ,  H01J2237/28

Abstract: A sample holder used in SEM (scanning electron microscopy) or TEM (transmission electron microscopy) permitting observation and inspection at higher resolution. The holder has a frame-like member provided with an opening that is covered with a film. The film has a first surface on which a sample is held. The thickness D of the film and the length L of the portion of the film providing a cover over the opening in the frame-like member satisfy a relationship given by L/D

Abstract translation: 用于SEM（扫描电子显微镜）或TEM（透射电子显微镜）中的样品架，允许以更高分辨率观察和检查。 保持器具有设置有被膜覆盖的开口的框架状构件。 该膜具有保持样品的第一表面。 膜的厚度D和在框状构件的开口上设置盖的膜的部分的长度L满足由L / D <200,000给出的关系。

公开(公告)号：US20100243888A1

公开(公告)日：2010-09-30

申请号：US12725816

申请日：2010-03-17

Applicant: Hidetoshi Nishiyama ,  Mitsuru Koizumi ,  Mitsuo Suga

Inventor： Hidetoshi Nishiyama ,  Mitsuru Koizumi ,  Mitsuo Suga

IPC: G01N23/22 ,  H01J37/26

CPC classification number: H01J37/20 ,  H01J37/244 ,  H01J2237/2003 ,  H01J2237/2004 ,  H01J2237/2443 ,  H01J2237/24475

Abstract: An inspection apparatus and method capable of well observing or inspecting a specimen contained in a liquid. The inspection apparatus has a film including first and second surfaces. Furthermore, the apparatus has a vacuum chamber for reducing the pressure in the ambient in contact with the second surface of the film, primary beam irradiation column connected with the vacuum chamber, and a shutter for partially partitioning the space between the film and the primary beam irradiation column within the vacuum chamber. A liquid sample is held on the first surface of the film. The primary beam irradiation column irradiates the sample. Backscattered electrons (a secondary beam) produced from the sample by the primary beam irradiation are directed at the shutter, producing secondary electrons (a tertiary signal).

Abstract translation: 能够良好地观察或检查包含在液体中的试样的检查装置和方法。 检查装置具有包括第一表面和第二表面的膜。 此外，该装置具有用于减小与膜的第二表面接触的环境中的压力的​​真空室，与真空室连接的主光束照射塔，以及用于部分地分隔膜和主光束之间的空间的遮板 真空室内的照射柱。 液体样品被保持在膜的第一表面上。 主光束照射列照射样品。 通过主光束照射由样品产生的后向散射电子（次级光束）指向快门，产生二次电子（第三信号）。

公开(公告)号：US20100051803A1

公开(公告)日：2010-03-04

申请号：US12546069

申请日：2009-08-24

Applicant: Mitsuru Koizumi ,  Hidetoshi Nishiyama

Inventor： Mitsuru Koizumi ,  Hidetoshi Nishiyama

IPC: G01N23/00

CPC classification number: H01J37/28 ,  H01J2237/022 ,  H01J2237/0268 ,  H01J2237/2004 ,  H01J2237/2608

Abstract: A particle beam system is offered which can prevent contamination of the inside of the objective lens, the objective lens being located at the front end of the optical column. The particle beam system has an optical column equipped with a particle beam source for emitting a particle beam and a beam passage pipe through which the beam passes. The system further includes a vacuum chamber connected with the front end portion of the column. The beam passed through the pipe is released from the front end of the column. An inner pipe is detachably disposed inside the beam passage pipe located at the front-end side of the column.

Abstract translation: 提供一种粒子束系统，其可以防止物镜内部的污染，物镜位于光学柱的前端。 粒子束系统具有装备有用于发射粒子束的粒子束源和光束通过的射束通过管的光学柱。 该系统还包括与柱的前端部连接的真空室。 通过管道的梁从柱的前端释放。 内管可拆卸地设置在位于塔的前端侧的梁通道管内。

公开(公告)号：US07482587B1

公开(公告)日：2009-01-27

申请号：US11600398

申请日：2006-11-15

Applicant: Dudley Sean Finch

Inventor： Dudley Sean Finch

IPC: G01N1/28 ,  G01N13/16 ,  B01D63/00

CPC classification number: H01J37/26 ,  H01J37/20 ,  H01J2237/20 ,  H01J2237/2002 ,  H01J2237/2004

Abstract: The present invention disclosure relates to the use of a silicon substrate with a thin film membrane as a transparent substrate for the imaging of biological- and material-related specimens using a microscope such as a transmission electron microscope (TEM). More specifically, the present invention relates to an improved substrate design that incorporates the fabrication of a circular shape that allows easier insertion into traditional specimen holders used in TEMs. In addition to an improved shape, the present invention incorporates microscopic surface texture on the gripping surface that assists in handling. The invention also encompasses surface modification techniques for enhanced biocompatibility of the thin film membrane for biomedical applications.

Abstract translation: 本发明涉及使用具有薄膜膜的硅衬底作为透明衬底的用途，用于使用透射电子显微镜（TEM）等显微镜对生物和材料相关样品进行成像。 更具体地，本发明涉及一种改进的基板设计，其包括制造圆形形状，其允许更容易地插入到用于TEM中的传统样品保持器中。 除了改进的形状之外，本发明还在辅助处理的抓握表面上结合微观表面纹理。 本发明还包括用于增强用于生物医学应用的薄膜膜的生物相容性的表面改性技术。

公开(公告)号：US20070145287A1

公开(公告)日：2007-06-28

申请号：US11452333

申请日：2006-06-14

Applicant: Chih-Yu Chao ,  Wen-Jiunn Hsieh

Inventor： Chih-Yu Chao ,  Wen-Jiunn Hsieh

IPC: G21K5/10

CPC classification number: H01J37/20 ,  H01J2237/188 ,  H01J2237/2004 ,  H01J2237/262

Abstract: A specimen box for an electron microscope capable of observing a general specimen or a live cell is formed of a housing. The housing includes a receiving chamber formed therein and at least one view hole formed on each of a top side thereof and a bottom side thereof and communicating with the receiving chamber and coaxially aligned with the other. The distance between a bottom end of the view hole located on the top side of the housing and a top end of the view hole located on the bottom side of the housing is smaller than 50 μm. The housing is ultra-thin to enable penetration of the electron beam therethrough even though a liquid specimen is injected into the housing. A general specimen or a live cell can be put into the housing for the microscopic observation under the electron microscope.

Abstract translation: 用于观察一般样品或活细胞的电子显微镜的标本盒由壳体形成。 壳体包括形成在其中的接收室和形成在其顶侧和底侧中的每一个上的至少一个视孔，并与接收室连通并与另一个同轴对准。 位于壳体顶部的视野孔的底端与位于壳体底侧的视野孔的顶端之间的距离小于50μm。 壳体是超薄的，以使得即使将液体样本注入到壳体中也能够穿过电子束。 一般标本或活细胞可以放入外壳中进行显微镜观察。

公开(公告)号：US11994663B2

公开(公告)日：2024-05-28

申请号：US16960287

申请日：2019-01-07

Applicant: HENNYZ B.V.

Inventor： Hendrik Willem Zandbergen

IPC: G02B21/32 ,  H01J37/20

CPC classification number: G02B21/32 ,  H01J37/20 ,  H01J2237/2001 ,  H01J2237/2004 ,  H01J2237/204 ,  H01J2237/2802

Abstract: The present invention is in the field of a vacuum transfer assembly, such as for cryotransfer, and specifically a TEM vacuum transfer assembly, which can be used in microscopy, a sample holder, a vacuum housing, a sample holder stage and a sample holder coupling unit for use in the assembly, and a microscope comprising said assembly as well as a method of vacuum transfer into a microscope.

公开(公告)号：US20170205363A1

公开(公告)日：2017-07-20

申请号：US15314141

申请日：2015-06-18

Applicant: LEIBNIZ-INSTITUT FUER NEUE MATERIALIEN GEMEINNUETZIGE GESELLSCHAFT MIT BESCHRAENKTER HAFT

Inventor： Niels DE JONGE ,  Diana B. PECKYS

IPC: G01N23/225 ,  H01J37/20

CPC classification number: G01N23/2251 ,  G01N2223/307 ,  G01N2223/418 ,  G01N2223/612 ,  H01J37/20 ,  H01J37/228 ,  H01J2237/2004 ,  H01J2237/24455 ,  H01J2237/2802

Abstract: The present invention relates to a device and a method for the stoichiometric analysis of samples.In order to study the spatial distribution of different proteins in the plasma membrane of a complete cell within a short time frame, a device and a method are proposed for the stoichiometric analysis of samples.The object is established by means of a device for the stoichiometric analysis of samples, said device comprising a) a sample processing device comprising a sample holder for holding the sample, means for setting the temperature, means for adding and removing fluids (including gases) and at least one fluid reservoir, b) an electron microscope with a detector, and c) a computer-controlled process control system for controlling the means for setting the temperature and the means for adding and removing fluids (including gases), a computer-controlled and automated imaging device that captures images by means of the electron microscope, a unit that stores the captured images and an image analysis unit controlled by the computer.

公开(公告)号：US20160042912A1

公开(公告)日：2016-02-11

申请号：US14817551

申请日：2015-08-04

Applicant: Vivekananda P. Adiga ,  Gabriel Dunn ,  Alexander K. Zettl ,  A. Paul Alivisatos

Inventor： Vivekananda P. Adiga ,  Gabriel Dunn ,  Alexander K. Zettl ,  A. Paul Alivisatos

IPC: H01J37/20 ,  G02B1/12

CPC classification number: H01J37/20 ,  C23C18/08 ,  G02B1/12 ,  G02B21/16 ,  H01J37/26 ,  H01J2237/2003 ,  H01J2237/2004 ,  H01L29/1606 ,  H01L29/66045 ,  Y10T29/49002

Abstract: This disclosure provides systems, methods, and apparatus related to liquid flow cells for microscopy. In one aspect, a device includes a substrate having a first and a second oxide layer disposed on surfaces of the substrate. A first and a second nitride layer are disposed on the first and second oxide layers, respectively. A cavity is defined in the first oxide layer, the first nitride layer, and the substrate, with the cavity including a third nitride layer disposed on walls of the substrate and the second oxide layer that define the cavity. A channel is defined in the second oxide layer. An inlet port and an outlet port are defined in the second nitride layer and in fluid communication with the channel. A plurality of viewports is defined in the second nitride layer. A first graphene sheet is disposed on the second nitride layer covering the plurality of viewports.

Abstract translation: 本公开提供了与用于显微镜的液体流动池相关的系统，方法和装置。 一方面，一种器件包括具有设置在衬底表面上的第一和第二氧化物层的衬底。 第一和第二氮化物层分别设置在第一和第二氧化物层上。 在第一氧化物层，第一氮化物层和衬底中限定空腔，空腔包括设置在衬底的壁上的第三氮化物层和限定空腔的第二氧化物层。 在第二氧化物层中限定通道。 入口端口和出口限定在第二氮化物层中并与通道流体连通。 在第二氮化物层中限定多个视口。 第一石墨烯片被布置在覆盖多个视口的第二氮化物层上。

公开(公告)号：US20130149459A1

公开(公告)日：2013-06-13

申请号：US13807888

申请日：2011-07-01

Applicant: Pascal Bruna ,  Denis Busardo

Inventor： Pascal Bruna ,  Denis Busardo

IPC: B01J19/08

CPC classification number: B01J19/085 ,  A61M15/00 ,  A61M2205/0205 ,  B05B11/0005 ,  B65D81/24 ,  B65D83/75 ,  C03C23/0055 ,  C08J7/123 ,  C08J7/18 ,  C23C14/48 ,  H01J37/3171 ,  H01J2237/2004

Abstract: A method of surface treating a fluid dispenser device, the method including a step of modifying, by ion implantation using multi-charged and multi-energy ion beams, at least one surface to be treated of at least a portion of the device in contact with the fluid. The modified surface has properties limiting the formation of a biofilm and thus the appearance and/or proliferation of bacteria on the modified surface, the multi-charged ions being selected from helium, boron, carbon, nitrogen, oxygen, neon, argon, krypton, and xenon, ionic implantation being carried out to a depth of 0 μm to 3 μm.

Abstract translation: 一种表面处理流体分配装置的方法，该方法包括通过使用多电荷和多能离子束的离子注入来修饰至少一个待处理表面的步骤，所述至少一个待处理表面与装置的至少一部分接触 流体。 改性表面具有限制生物膜的形成，从而限制细菌在改性表面上的外观和/或增殖的性质，多电荷离子选自氦，硼，碳，氮，氧，氖，氩，氪， 并且氙，离子注入进行到0〜3μm的深度。

公开(公告)号：US20120120226A1

公开(公告)日：2012-05-17

申请号：US13299241

申请日：2011-11-17

Applicant: Niels de Jonge

Inventor： Niels de Jonge

IPC: H04N7/18

CPC classification number: G01N23/2204 ,  B01L3/502715 ,  G01N23/2251 ,  H01J37/20 ,  H01J2237/2003 ,  H01J2237/2004 ,  H01J2237/2802

Abstract: In one aspect, the present invention relates to a microfluidic chamber. In one embodiment, the microfluidic chamber has a first sub-chamber and at least one second sub-chamber. The first sub-chamber has a first window and a second window. Both the first window and the second window are transparent to electrons of certain energies. The second window is positioned substantially parallel and opposite to the first window defining a first volume therebetween. The first window and the second window are separated by a distance that is sufficiently small such that an electron beam that enters from the first window can propagate through the first sub-chamber and exit from the second window. The at least one second sub-chamber is in fluid communication with the first sub-chamber and has a second volume that is greater than the first volume of the first sub-chamber.

Abstract translation: 一方面，本发明涉及一种微流体室。 在一个实施例中，微流体室具有第一子室和至少一个第二子室。 第一子室具有第一窗口和第二窗口。 第一窗口和第二窗口对于某些能量的电子是透明的。 第二窗口基本平行且相对于第一窗口定位，在第一窗口之间限定第一容积。 第一窗口和第二窗口被分开足够小的距离，使得从第一窗口进入的电子束可以传播通过第一子室并从第二窗口排出。 所述至少一个第二子室与所述第一子室流体连通并且具有大于所述第一子室的第一容积的第二容积。