公开(公告)号：US20180334699A1

公开(公告)日：2018-11-22

申请号：US15979230

申请日：2018-05-14

Applicant: Abbott Laboratories

Inventor： Jiong Wu ,  Emily H. Lin ,  Jihping Yang

IPC: C12Q1/04 ,  G01N33/50

CPC classification number: C12Q1/04 ,  G01N33/5094

Abstract: Herein is provided a simple, reliable and accurate method for cellular analysis on hematology analyzers. In various aspects, the methods provide separation and/or differentiation between red blood cells (RBCs) and white blood cells (WBCs) by utilizing a fluorescent dye to selectively stain WBCs such that they emit stronger fluorescence signals. The method provides optimal detection limits on WBCs and RBCs, thereby allowing analysis of samples with sparse cellular concentrations. As few as one reagent may be used to prepare a single dilution for body fluid analysis, in order to simplify the body fluid analysis. Minimal damage to WBCs is attained using the lysis-free approach described in aspects of the disclosure.

公开(公告)号：US20180128731A1

公开(公告)日：2018-05-10

申请号：US15704972

申请日：2017-09-14

Applicant: Abbott Laboratories

Inventor： Jiong Wu ,  Giacomo Vacca

IPC: G01N15/14 ,  G01N21/64 ,  G01N33/49 ,  G01N15/00 ,  G01N15/10

CPC classification number: G01N15/1434 ,  G01N15/1459 ,  G01N21/6428 ,  G01N33/49 ,  G01N2015/008 ,  G01N2015/1006 ,  G01N2015/1477 ,  G01N2015/1488 ,  G01N2021/6439

Abstract: Systems and methods for analyzing blood samples, and more specifically for performing a white blood cell (WBC) differential analysis. The systems and methods screen WBCs by means of fluorescence staining and a fluorescence triggering strategy. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder WBC reagent(s), suitable for assays of samples containing fragile WBCs. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye, which corresponds in emission spectrum to an excitation source of a hematology instrument; (b) using a fluorescence trigger to screen the blood sample for WBCs; and (c) using measurements of (1) axial light loss, (2) intermediate angle scatter, (3) 90° polarized side scatter, (4) 90° depolarized side scatter, and (5) fluorescence emission to perform a differentiation analysis.

公开(公告)号：US09638621B2

公开(公告)日：2017-05-02

申请号：US15044636

申请日：2016-02-16

Applicant: Abbott Laboratories

Inventor： Martin Krockenberger ,  Jiong Wu ,  Bodo Roemer ,  Giacomo Vacca

IPC: G01N31/00 ,  G01N15/14 ,  G01N15/00 ,  G01N21/53 ,  G01N33/49 ,  G01N15/10

CPC classification number: G01N15/1404 ,  G01N15/00 ,  G01N15/1429 ,  G01N15/1459 ,  G01N21/53 ,  G01N33/49 ,  G01N33/4915 ,  G01N33/492 ,  G01N2015/0073 ,  G01N2015/0076 ,  G01N2015/008 ,  G01N2015/0084 ,  G01N2015/1006 ,  G01N2015/1486 ,  G01N2800/22 ,  Y10T436/101666

Abstract: A method for identifying, analyzing, and quantifying the cellular components of whole blood by means of an automated hematology analyzer and the detection of the light scattered, absorbed, and fluorescently emitted by each cell. More particularly, the aforementioned method involves identifying, analyzing, and quantifying the cellular components of whole blood by means of a light source having a wavelength ranging from about 400 nm to about 450 nm and multiple in-flow optical measurements and staining without the need for lysing red blood cells.

公开(公告)号：US09267931B2

公开(公告)日：2016-02-23

申请号：US14562073

申请日：2014-12-05

Applicant: Abbott Laboratories

Inventor： Martin Krockenberger ,  Jiong Wu ,  Bodo Roemer ,  Giacomo Vacca

IPC: G01N33/00 ,  G01N31/00 ,  G01N33/49 ,  G01N15/00 ,  G01N21/53 ,  G01N15/14

CPC classification number: G01N15/1404 ,  G01N15/00 ,  G01N15/1429 ,  G01N15/1459 ,  G01N21/53 ,  G01N33/49 ,  G01N33/4915 ,  G01N33/492 ,  G01N2015/0073 ,  G01N2015/0076 ,  G01N2015/008 ,  G01N2015/0084 ,  G01N2015/1006 ,  G01N2015/1486 ,  G01N2800/22 ,  Y10T436/101666

Abstract: A method for identifying, analyzing, and quantifying the cellular components of whole blood by means of an automated hematology analyzer and the detection of the light scattered, absorbed, and fluorescently emitted by each cell. More particularly, the aforementioned method involves identifying, analyzing, and quantifying the cellular components of whole blood by means of a light source having a wavelength ranging from about 400 nm to about 450 nm and multiple in-flow optical measurements and staining without the need for lysing red blood cells.

Abstract translation: 一种通过自动血液分析仪识别，分析和定量全血细胞成分的方法，以及每个细胞散射，吸收和荧光发射的光的检测。 更具体地，上述方法包括通过具有波长范围为约400nm至约450nm的光源和多次流入光学测量和染色来鉴定，分析和定量全血的细胞组分，并且不需要 裂解红细胞。

公开(公告)号：US20150160189A1

公开(公告)日：2015-06-11

申请号：US14562073

申请日：2014-12-05

Applicant: Abbott Laboratories

Inventor： Martin Krockenberger ,  Jiong Wu ,  Bodo Roemer ,  Giacomo Vacca

IPC: G01N33/49 ,  G01N21/53

CPC classification number: G01N15/1404 ,  G01N15/00 ,  G01N15/1429 ,  G01N15/1459 ,  G01N21/53 ,  G01N33/49 ,  G01N33/4915 ,  G01N33/492 ,  G01N2015/0073 ,  G01N2015/0076 ,  G01N2015/008 ,  G01N2015/0084 ,  G01N2015/1006 ,  G01N2015/1486 ,  G01N2800/22 ,  Y10T436/101666

Abstract: A method for identifying, analyzing, and quantifying the cellular components of whole blood by means of an automated hematology analyzer and the detection of the light scattered, absorbed, and fluorescently emitted by each cell. More particularly, the aforementioned method involves identifying, analyzing, and quantifying the cellular components of whole blood by means of a light source having a wavelength ranging from about 400 nm to about 450 nm and multiple in-flow optical measurements and staining without the need for lysing red blood cells.

Abstract translation: 一种通过自动血液分析仪识别，分析和定量全血细胞成分的方法，以及每个细胞散射，吸收和荧光发射的光的检测。 更具体地，上述方法包括通过具有波长范围为约400nm至约450nm的光源和多次流入光学测量和染色来鉴定，分析和定量全血的细胞组分，并且不需要 裂解红细胞。

公开(公告)号：US20140273061A1

公开(公告)日：2014-09-18

申请号：US14137392

申请日：2013-12-20

Applicant: Abbott Laboratories

Inventor： Jiong Wu ,  Ji Lin

IPC: G01N33/80

CPC classification number: G01N33/56972 ,  G01N15/0205 ,  G01N15/1434 ,  G01N15/1459 ,  G01N21/47 ,  G01N33/49 ,  G01N2015/0073 ,  G01N2015/0076 ,  G01N2015/0084 ,  G01N2015/0277 ,  G01N2015/1006 ,  G01N2015/1477 ,  G01N2015/1493

Abstract: Aspects of the invention include hematology analysis reagents, systems and methods that can be used to preserve blood cell morphology and integrity as well as provide sample integrity and optical clarity to facilitate optical analysis of blood samples. In some embodiments, the reagents include a non-phosphate organic buffer and a sphering surfactant. The pH and osmolality of the reagents may be adjusted to desired ranges. In addition, the reagents can be simply diluted with de-ionized water prior to use.

Abstract translation: 本发明的方面包括可用于保持血细胞形态和完整性的血液学分析试剂，系统和方法，以及提供样品完整性和光学透明度以促进血液样品的光学分析。 在一些实施方案中，试剂包括非磷酸盐有机缓冲剂和球形表面活性剂。 试剂的pH和重量克分子渗透浓度可以调节到所需的范围。 此外，试剂可以在使用前用去离子水简单稀释。