公开(公告)号：US20020127634A1

公开(公告)日：2002-09-12

申请号：US08463404

申请日：1995-06-05

Inventor： MICHAEL D. WEST ,  JERRY SHAY ,  WOODRING WRIGHT ,  ELIZABETH H. BLACKBURN

IPC: G01N031/00 ,  G01N035/00 ,  G01N035/02 ,  G01J003/42

CPC classification number: C12N15/10 ,  A61K31/522 ,  A61K31/70 ,  A61K31/7076 ,  A61K31/711 ,  A61K38/00 ,  C12N5/0018 ,  C12N5/163 ,  C12N9/1241 ,  C12N15/113 ,  C12N15/1137 ,  C12N2501/70 ,  C12N2510/04 ,  C12Q1/48 ,  C12Q1/68 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6876 ,  C12Q1/6886 ,  C12Q1/703 ,  C12Q2600/112 ,  C12Q2600/136 ,  C12Y207/07049 ,  G01N2333/91245 ,  Y02A50/409 ,  Y02A50/411 ,  Y10S977/773 ,  Y10S977/801 ,  Y10S977/918 ,  Y10S977/927 ,  Y10T436/10 ,  Y10T436/105831 ,  Y10T436/11 ,  Y10T436/115831 ,  C12Q2521/113

Abstract: Method and compositions are provided for the determination of telomere length and telomerase activity, as well as the ability to inhibit telomerase activity in the treatment of proliferative diseases. Particularly, primers are elongated under conditions which minimize interference from other genomic sequences, so as to obtain accurate determinations of telomeric length or telomerase activity. In addition, compositions are provided for intracellular inhibition of telomerase activity and means are shown for slowing the loss of telomeric repeats in aging cells.

Abstract translation: 提供了测定端粒长度和端粒酶活性的方法和组合物，以及抑制端粒酶活性治疗增殖性疾病的能力。 特别地，引物在使来自其它基因组序列的干扰最小化的条件下伸长，以获得端粒长度或端粒酶活性的准确测定。 此外，提供组合物用于细胞内抑制端粒酶活性，并且显示了用于减缓衰老细胞中端粒重复的丧失的手段。

公开(公告)号：US20020085200A1

公开(公告)日：2002-07-04

申请号：US09862881

申请日：2001-08-09

Inventor： Craig M. Herzinger ,  Steven E. Green ,  Ronald A. Synowicki

IPC: G01J003/42

CPC classification number: G01J3/28 ,  G01J3/42 ,  G01N21/274

Abstract: Disclosed are spectrophotometer systems and methodology for obtaining data of improved precision therefrom, including replacement of data determined to be suspect based on comparison of multiple baselines.

Abstract translation: 公开了分光光度计系统和方法，用于从其获得改进精度的数据，包括基于多个基线的比较来替换被确定为可疑的数据。

公开(公告)号：US20010050770A1

公开(公告)日：2001-12-13

申请号：US09752733

申请日：2001-01-03

Applicant: SHIMADZU CORPORATION

Inventor： Kazumi Yokota

IPC: G01J003/42

CPC classification number: G01J3/42 ,  G01J3/08

Abstract: In a spectrophotometer, light emitted from a light source is processed to provide monochromatic light by a monochromator, and reaches a sector mirror having a chopper function. The sector mirror is rotated by a motor, and a rotational frequency of the motor can be controlled by a controller. By rotation of the sector mirror, monochromatic light is alternately distributed to a sample cell and a reference cell through a mirror. In measuring a wavelength range where a detector having a photomultiplier with a fast response speed is used, the motor is rotated at a high speed, so that time for scanning a wavelength is shortened. In measuring a wavelength range where a detector having lead sulfide with a slow response speed is used, the motor is rotated at a low speed to obtain a sufficiently strong signal intensity. Accordingly, in the spectrophotometer, the analysis time can be shortened, and the sufficiently strong signal can be outputted at the same time.

Abstract translation: 在分光光度计中，对从光源发出的光进行处理，由单色仪提供单色光，并到达具有斩波功能的扇形镜。 扇形镜由电动机旋转，电动机的转速可由控制器控制。 通过扇形镜的旋转，单色光通过反射镜交替地分布到样品池和参考池。 在测量使用具有快速响应速度的光电倍增管的检测器的波长范围时，马达以高速旋转，从而缩短了扫描波长的时间。 在测量使用具有缓慢响应速度的具有硫化铅的检测器的波长范围时，马达以低速旋转以获得足够强的信号强度。 因此，在分光光度计中，能够缩短分析时间，能够同时输出足够强的信号。

公开(公告)号：US20040246477A1

公开(公告)日：2004-12-09

申请号：US10800320

申请日：2004-03-11

Inventor： John A. Moon ,  James S. Sirkis ,  Ralph Jones ,  Charles R. Winston ,  David R. Fournier ,  Joseph Pinto ,  Robert N. Brucato ,  James R. Dunphy ,  Christopher J. Chestnut

IPC: G01J003/00 ,  G01J003/42

CPC classification number: G01J3/06 ,  G01J3/02 ,  G01J3/0202 ,  G01J3/0208 ,  G01J3/021 ,  G01J3/0218 ,  G01J3/0256 ,  G01J3/0291 ,  G01J3/18 ,  G01J3/28 ,  G01J3/32 ,  G01J3/36 ,  G01J2003/064 ,  G01J2003/1819 ,  G02B6/2931 ,  G02B6/4215 ,  H04B10/077 ,  H04B10/07955 ,  H04Q2011/0026 ,  H04Q2011/0049 ,  H04Q2011/0083

Abstract: An optical spectrum analyzer (OSA) 10 sequentially or selectively samples (or filters) a spectral band(s) 11 of light from a broadband optical input signal 12 and measures predetermined optical parameters of the optical signal (e.g., spectral profile) of the input light 12. The OSA 10 is a free-space optical device that includes a collimator assembly 15, a diffraction grating 20 and a mirror 22. A launch pigtail emits into free space the input signal through the collimator assembly 15 and onto the diffraction grating 20, which separates or spreads spatially the collimated input light, and reflects the dispersed light onto the mirror 22. A null/4 plate 26 is disposed between the mirror 22 and the diffraction grating 20. The mirror reflects the separated light back through the null/4 plate 26 to the diffraction grating 20, which reflects the light back through the collimating lens 18. The lens 18 focuses spectral bands of light (null1-nullN) at different focal points in space. One of the spectral bands 11 is focused onto a receive pigtail 28, which then propagates to a photodetector 30. A pivoting mechanism 34 pivots the diffraction grating 20 or mirror 22 about a pivot point 36 to sequentially or selectively focus each spectral band 11 to the receive pigtail 28. A position sensor 42 detects the displacement of the diffraction grating 24 or mirror.

Abstract translation: 光谱分析仪（OSA）10顺序地或选择性地从宽带光输入信号12采样（或滤波）光谱带11，并且测量输入的光信号的光学参数（例如，光谱分布） OSA 10是自由空间光学装置，其包括准直器组件15，衍射光栅20和反射镜22.发射尾纤通过准直器组件15将信号发射到自由空间中，并将其输入到衍射光栅20上 ，其在空间上分离或扩展准直的输入光，并将分散的光反射到反射镜22上.λ/ 4板26设置在反射镜22和衍射光栅20之间。反射镜将分离的光反射回λ/ 4板26连接到衍射光栅20，其通过准直透镜18反射光。透镜18将光（λ1-λN）的光谱带聚焦在空间中的不同焦点处。 光谱带11中的一个聚焦在接收引线28上，接收引线28然后传播到光电检测器30.枢转机构34围绕枢转点36枢转衍射光栅20或反射镜22，以顺序地或选择性地将每个光谱带11聚焦到 接收尾纤28.位置传感器42检测衍射光栅24或反射镜的位移。

公开(公告)号：US20040239926A1

公开(公告)日：2004-12-02

申请号：US10445570

申请日：2003-05-27

Inventor： Alex Dimitri Sokolowski ,  Walter van Kruijsen

IPC: G01J003/42

CPC classification number: G01J3/42 ,  G01N21/11 ,  G01N21/3563 ,  G01N21/85 ,  G01N2021/8592

Abstract: A sampling system for performing a spectroscopic analysis on a polymer during its production is described. The system includes a transfer tube capable of transporting the polymer from a production site to a storage site. The tube includes an entry site connected to the production site; and an exit site connected to the storage site. A transparent window is inserted into the wall of the tube, and a door or other barrier is provided to stop the flow of polymer through the tube, so that it accumulates against the window. A spectroscopic device situated outside the tube then takes a reading of the polymer through the window, to perform the desired analysis. Related methods are also described.

Abstract translation: 描述了在其生产期间对聚合物进行光谱分析的采样系统。 该系统包括能够将聚合物从生产现场运输到储存场所的转运管。 管子包括连接到生产现场的入口点; 以及连接到存储站点的出口站点。 透明窗口插入管的壁中，并且提供门或其他屏障以阻止聚合物流过管，使得其积聚在窗户上。 位于管外的光谱装置然后通过窗口读取聚合物，以进行所需的分析。 还描述了相关方法。

公开(公告)号：US20030202179A1

公开(公告)日：2003-10-30

申请号：US10135854

申请日：2002-04-29

Applicant: The Curators of the University of Missouri

Inventor： David W. Larsen ,  Zhi Xu ,  Wayne Garver

IPC: G01J003/42

CPC classification number: G01J3/28 ,  G01J3/02 ,  G01J3/0224 ,  G01J3/0286 ,  G01J3/0291 ,  G01J3/42

Abstract: The invention concerns improvements in spectrophotometry. Aspects of the invention may be used independently or together to increase the sensitivity of spectrophotometry. One aspect of the invention is a spectrophotometer detection circuit. In this aspect of the invention, currents attributable to reference and sample beams are cancelled in the current mode. The detection circuit produces a first voltage proportional to the difference in currents and a second voltage proportional to one of the reference or sample beams. Both voltages are available to allow simultaneous measurement and analysis. Another aspect of the invention concerns thermal stability. According to the invention, thermal conductivity is established among the housing and optical system components to promote equilibrium. Another preferred embodiment has a unitary solid metal housing with a hollowed portion defined to mount and place optical system components. Recognition and identification of important noise sources in spectrophotometers forms an aspect of the invention contributing to the features and combinations of features in preferred embodiments. Many noise sources would not normally be considered in conventional spectrophotometry because the magnitude of particular noise sources dominates device performance.

Abstract translation: 本发明涉及分光光度法的改进。 本发明的各方面可以独立地或一起使用来提高分光光度法的灵敏度。 本发明的一个方面是分光光度计检测电路。 在本发明的这个方面，在当前模式下，归因于参考和采样光束的电流被消除。 检测电路产生与电流差成比例的第一电压和与参考或采样光束之一成比例的第二电压。 两种电压均可用于同时测量和分析。 本发明的另一方面涉及热稳定性。 根据本发明，在壳体和光学系统部件之间建立导热性以促进平衡。 另一个优选实施例具有整体的实心金属外壳，其具有限定为安装和放置光学系统部件的中空部分。 识别和识别分光光度计中的重要噪声源形成本发明的一个方面，其有利于优选实施例中的特征和特征的组合。 常规分光光度法通常不会考虑许多噪声源，因为特定噪声源的大小主导器件性能。

公开(公告)号：US20030169421A1

公开(公告)日：2003-09-11

申请号：US10303335

申请日：2002-11-25

Inventor： Peter Ehbets

IPC: G01J003/42

CPC classification number: G01J3/50 ,  G01J3/02 ,  G01J3/0208 ,  G01J3/021 ,  G01J3/024 ,  G01J3/0256 ,  G01J3/0272 ,  G01J3/0278 ,  G01J3/0291 ,  G01J3/10 ,  G01J3/501 ,  G01N21/251 ,  G01N21/255 ,  G01N2021/4757 ,  G01N2201/062

Abstract: A spectrophotometer for measuring light received from a sample is disclosed. The spectrophotometer includes a measurement head housing a sample and an illumination arrangement having a light source essentially continuous in the visible spectral range, formed by light-emitting diodes. The measurement head includes an illumination channel for each light emitting diode directing the light from each diode at a defined angle of incidence onto a measurement spot on the sample and a collecting arrangement capturing the light originating from the measurement spot. A spectrometer is optically connected to the collecting arrangement for splitting the captured light into its spectral components and for generating corresponding electrical signals. An electronic circuit, constructed for selectively controlling the light emitting diodes, controls the illumination arrangement and the spectrometer and processes the electrical measurement signals. A color densitometer, a variant of a spectorphotometer constructed in accordance with the subject disclosure, is also disclosed.

Abstract translation: 公开了一种用于测量从样品接收的光的分光光度计。 分光光度计包括容纳样品的测量头和具有由可见光谱范围内基本上连续的光源的照明装置，其由发光二极管形成。 测量头包括用于每个发光二极管的照明通道，其将来自每个二极管的光以规定的入射角度引导到样品上的测量点上，以及收集装置捕获源自测量点的光。 光谱仪光学连接到采集装置，用于将捕获的光分解成其光谱分量并产生相应的电信号。 构造用于选择性地控制发光二极管的电子电路控制照明装置和光谱仪并处理电测量信号。 还公开了彩色浓度计，根据本公开内容构造的分光光度计的变体。

公开(公告)号：US20030081204A1

公开(公告)日：2003-05-01

申请号：US10226592

申请日：2002-08-23

Inventor： Paul J. Cronin ,  Peter J. Miller

IPC: G01J003/42 ,  G01J003/00 ,  G01N021/64

CPC classification number: G01N21/645 ,  G01N21/31 ,  G01N2021/6421

Abstract: A method is described that includes measuring, at each of a set of W wavelength bins, a spectral response of at least one region of a sample stained with multiple stains, and determining the concentration of at least one of the stains in the region of the sample based in part on the spectral responses, the wavelength bins being chosen so that a matrix of elements that represent the responses of the stains at the wavelength bin has an inverse for which a mathematical stability is maximum relative to the inverses of other matrices of elements that represent the responses of the stains for other possible sets of wavelength bins.

Abstract translation: 描述了一种方法，其包括在一组W波长区段中的每一个处测量被多个污渍染色的样品的至少一个区域的光谱响应，以及确定在多个污渍区域中的至少一个污渍的浓度 样品部分地基于光谱响应，选择波长区，使得表示波长箱上的污点的响应的元素矩阵具有相对于元素的其它矩阵的逆的数学稳定性最大的逆矩阵 其代表着对于其它可能的波长箱组的污渍的响应。

公开(公告)号：US20020193948A1

公开(公告)日：2002-12-19

申请号：US10172563

申请日：2002-06-13

Applicant: CARL ZEISS JENA GmbH

Inventor： Dietrich Schweitzer ,  Lutz Leistritz ,  Martin Hammer ,  Karl-Heinz Donnerhacke

IPC: G06F019/00 ,  G01N033/48 ,  G01N033/50 ,  G06K009/00 ,  G01J003/42 ,  G01J003/427

CPC classification number: A61B3/12

Abstract: An arrangement and method for determining the two-dimensional distribution of fundus pigments, particularly of the xanthophyll macular pigment. The arrangement for carrying out the method comprises an illumination unit which illuminates the retina via an illumination beam path directed to the ocular fundus, observation optics located in the observation beam path proceeding from the ocular fundus, an image processing unit, elements for beam deflection and a central controlling and evaluating unit. In the method, a two-dimensional reflection image of the retina is recorded in a selected narrow-band wavelength region. In evaluating this two-dimensional reflection image, site-specific areas are established for determining the optical density and comparison values. The optical density of the fundus pigment at every fundus location is calculated from the negative logarithmic value of the quotient of the intensity value of the reflection image IR(null) at this fundus site to a comparison intensity value of the reflection image IR(null)Comparison. The suggested solution for the objective detection of the two-dimensional distribution of the optical density of the macular pigment xanthophyll is also suitable in principle for determining the distribution of other fundus pigments.

Abstract translation: 用于确定眼底颜料，特别是黄叶黄斑色素的二维分布的布置和方法。 用于执行该方法的装置包括：照明单元，其经由指向眼底的照明光束照射视网膜，位于观察光束路径中的观察光学器件，从眼底进入，图像处理单元，用于光束偏转的元件， 中央控制评估单位。 在该方法中，将视网膜的二维反射图像记录在所选择的窄带波长区域中。 在评估这种二维反射图像时，建立位置特定区域以确定光密度和比较值。 每个眼底位置的眼底颜料的光密度由该眼底位置的反射图像IR（lambd）的强度值的商数的负对数值与反射图像IR（lambd）的比较强度值计算， 比较。 用于目标检测黄斑色素叶黄素光密度二维分布的解决方案在原理上也适用于确定其他眼底颜料的分布。

公开(公告)号：US20020137052A1

公开(公告)日：2002-09-26

申请号：US09907795

申请日：2001-07-17

Applicant: Lynx Therapeutics, Inc.

Inventor： John Bridgham ,  Kevin P. Corcoran ,  George S. Golda ,  Sydney Brenner ,  Michael C. Pallas

IPC: C12Q001/68 ,  C12M001/34 ,  G01J003/42

CPC classification number: G01N21/6452 ,  B01J19/0046 ,  B01J2219/00274 ,  B01J2219/00317 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/10 ,  B01L2300/0877 ,  B01L2300/1822 ,  B01L2400/0403 ,  B01L2400/0475 ,  B01L2400/0622 ,  B01L2400/0644 ,  C12Q1/6874 ,  C40B60/14 ,  G01N15/1456 ,  G01N15/1475 ,  G01N15/1484 ,  G01N21/05 ,  G01N21/253 ,  G01N21/6428 ,  G01N2015/0015 ,  G01N2015/149 ,  G01N2021/0346 ,  C12Q2565/518 ,  C12Q2521/313 ,  C12Q2525/191 ,  C12Q2525/161

Abstract: An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array. A key feature of the invention is the correlation of the sequence of optical signals generated by each microparticle in the planar array during the analytical process.

Abstract translation: 提供了一种用于同时分析锚定到微粒的多个分析物的装置和系统。 每个具有单一类型分析物的均匀群体的微粒被布置为在流动室内部基本上固定的平面阵列，其中分析过程的步骤通过将一系列处理试剂通过流体系统递送到微粒进行， 微处理器控制。 响应于这样的处理步骤，在每个微粒的表面上产生光信号，其特征在于由微粒携带的分析物与递送的处理试剂之间的相互作用。多个分析物同时通过收集和记录 由平面阵列中的所有微粒产生的光信号。 本发明的一个关键特征是在分析过程中由平面阵列中的每个微粒产生的光信号序列的相关性。