中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

958 records (took 0.037 seconds)

    METHOD AND APPARATUS FOR ISOLATION, CAPTURE AND MOLECULAR ANALYSIS OF TARGET PARTICLES
    63.
    发明申请
    METHOD AND APPARATUS FOR ISOLATION, CAPTURE AND MOLECULAR ANALYSIS OF TARGET PARTICLES 审中-公开
    目标颗粒分离，捕获和分子分析的方法与装置

    公开(公告)号：US20160209299A1

    公开(公告)日：2016-07-21

    申请号：US14914714

    申请日：2014-08-28

    Applicant: APOCELL, INC.

    Inventor： Anoop MENACHERY ,  Vishal GUPTA ,  David K HASEGAWA ,  Ronald PETHIG

    IPC: G01N1/10 ,  G01N33/483 ,  G01N27/447

    CPC classification number: G01N1/10 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  G01N27/44717 ,  G01N27/44743 ,  G01N27/44769 ,  G01N30/0005 ,  G01N33/4833 ,  G01N2030/0065

    Abstract: Methods and apparatuses for target particle separation and isolation are disclosed. The apparatuses are flow chambers that include an infusion port configured to introduce a sample, and an elution flow port configured to receive an elution buffer. The flow chamber also includes an electrode coupled to a floor of the flow chamber, wherein the electrode includes at least a first zone with a first inter-electrode pitch and a second zone with a second inter-electrode pitch. The first zone generates a first electric field and the second zone generates a second electric field of a different magnitude than the first electric field when an electric signal is applied. The first electric field and the second electric field separate the target particle from the fluidic suspension via dielectrophoresis. The flow chamber also includes a waste disposal port that discharges the waste material, and a collection port configured that collects a target particle enriched sample.

    Abstract translation: 公开了用于目标颗粒分离和分离的方法和装置。 所述装置是流动室，其包括被配置为引入样品的输注端口和被配置为接收洗脱缓冲液的洗脱流动端口。 流动室还包括耦合到流动室的底板的电极，其中电极至少包括具有第一电极间距的第一区和具有第二电极间距的第二区。 第一区产生第一电场，并且当施加电信号时，第二区产生与第一电场不同大小的第二电场。 第一电场和第二电场通过介电电泳将目标颗粒与流体悬浮液分离。 流动室还包括排出废料的废物处理口，以及收集端口，其被配置为收集富含目标颗粒的样品。

    Multisort cell separation method
    66.
    发明申请
    Multisort cell separation method 审中-公开
    多重细胞分离法

    公开(公告)号：US20160186165A1

    公开(公告)日：2016-06-30

    申请号：US14944991

    申请日：2015-11-18

    Applicant: Miltenyi Biotec GmbH

    Inventor： Christian Dose ,  Volker Huppert ,  Burgund Kauling ,  Philipp Steinbrück

    IPC: C12N13/00 ,  G01N1/40

    CPC classification number: C12N13/00 ,  B03C1/01 ,  B03C1/288 ,  B03C2201/26 ,  C12N5/0087 ,  G01N1/40 ,  G01N33/54333 ,  G01N2001/4038

    Abstract: The invention is directed to a method for enriching target cells from a sample of cells characterized by: a) contacting the sample with a cell aggregation agent and first magnetic particles having an iron content of 0.1 pg to 5000 pg, coupled to a first antigen recognizing moiety; and second magnetic particles having an iron content of 0.05 fg to 100 fg and coupled to a second antigen recognizing moiety to obtain mixture a) b) applying a first magnetic field gradient to the mixture a) thereby removing the cells bound to the first antigen recognizing moiety coupled to the first magnetic particles, to obtain a mixture b) and obtaining an agglomerate comprising the cells of mixture a) bound to the cell aggregation agent c) applying a second magnetic field gradient to the mixture b) thereby immobilizing the cells bound to the second antigen recognizing moiety d) recovering the immobilized cells from the second magnetic field gradient as target cells.

    Abstract translation: 本发明涉及用于从细胞样品中富集靶细胞的方法，其特征在于：a）使样品与细胞聚集剂和铁含量为0.1pg至5000pg的第一磁性颗粒接触，第一磁性颗粒与第一抗原识别 部分; 和铁含量为0.05fg至100fg并与第二抗原识别部分偶联以获得混合物的第二磁性颗粒a）b）向混合物施加第一磁场梯度a）从而除去与第一抗原识别结合的细胞 与第一磁性颗粒结合的部分，以获得混合物b）并获得包含与细胞聚集剂结合的混合物a）的细胞的附聚物c）向混合物施加第二磁场梯度b）从而将结合到 第二抗原识别部分d）从作为靶细胞的第二磁场梯度回收固定化细胞。

    Separation of particles using multiplexed dielectrophoresis
    70.
    发明授权
    Separation of particles using multiplexed dielectrophoresis 有权
    使用多重介电电泳分离颗粒

    公开(公告)号：US09285338B2

    公开(公告)日：2016-03-15

    申请号：US13713802

    申请日：2012-12-13

    Applicant: University of Pittsburgh of the Commonwealth System of Higher Education

    Inventor： Samuel J. Dickerson ,  Steven P. Levitan ,  Donald M. Chiarulli

    IPC: G01N27/447 ,  B03C5/00 ,  B03C5/02

    CPC classification number: G01N27/447 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26

    Abstract: Systems, devices, and methods are presented that facilitate electronic manipulation and detection of submicron particles. Time-multiplexed dielectrophoresis can be employed by cycling between two or more disparate electric fields during separate portions of a duty cycle. By cycling between these two or more disparate electric fields, two or more disparate particle types can be separated from one another based on differences in electrical properties of the two or more disparate particle types.

    Abstract translation: 提出了促进亚微米颗粒的电子操作和检测的系统，装置和方法。 可以通过在占空比的分离部分期间在两个或更多个不同的电场之间循环来使用时间多路复用介电电泳。 通过在这两个或更多个不同的电场之间循环，基于两种或多种不同粒子类型的电性能的差异，可以将两种或更多种不同的粒子类型彼此分离。

Patent Agency Ranking