公开(公告)号：US07821634B2

公开(公告)日：2010-10-26

申请号：US12106233

申请日：2008-04-18

Applicant: Robert Dillon ,  Lee Grodzins ,  Stephanie Melikian

Inventor： Robert Dillon ,  Lee Grodzins ,  Stephanie Melikian

IPC: G01J3/30

CPC classification number: G01J3/443 ,  G01J3/02 ,  G01J3/0272 ,  G01N21/67 ,  G01N21/718

Abstract: Multiple energy sources, such as a laser and electrical current, are employed, in close coordination, spatially and temporally, to clean a sample, vaporize its material and excite vapor atoms for the purpose of atomic emission spectroscopy. These methods permit better monitoring and control of the individual processes in real time, lead to higher consistency and higher quality optical emission spectra, and enhance the measurements of non-conducting solids, liquids and gases. Additionally, a portable instrument is provided with both laser source and spectrometer optically coupled to a hand-holdable unit.

Abstract translation: 在诸如激光和电流之间的多个能源，在空间和时间上紧密协调地被采用以清洁样品，汽化其材料并激发蒸气原子以用于原子发射光谱。 这些方法允许实时更好地监测和控制各个过程，从而提高一致性和更高质量的光发射光谱，并增强非导电固体，液体和气体的测量。 此外，便携式仪器具有与手持式单元光学耦合的激光源和光谱仪。

公开(公告)号：US07764373B2

公开(公告)日：2010-07-27

申请号：US12067381

申请日：2006-09-20

Applicant: Takashi Fujii ,  Naohiko Goto ,  Megumi Miki ,  Takuya Nayuki ,  Koshichi Nemoto ,  Nobuyuki Tanaka

Inventor： Takashi Fujii ,  Naohiko Goto ,  Megumi Miki ,  Takuya Nayuki ,  Koshichi Nemoto ,  Nobuyuki Tanaka

IPC: G01J3/30

CPC classification number: G01N21/718

Abstract: Fine particles such as nanoparticles and microparticles is irradiated to generate plasma by focusing an ultrashort pulse laser beam 15 emitted from a laser device 16. More preferably, the plasma is generated by a filament 14 generated in the ultrashort pulse laser beam 15. A constituent of the fine particles is measured based on an emission spectrum from the plasma.

Abstract translation: 通过聚焦从激光装置16发射的超短脉冲激光束15照射细粒，例如纳米颗粒和微粒，以产生等离子体。更优选地，等离子体由在超短脉冲激光束15中产生的细丝14产生。 基于来自等离子体的发射光谱来测量细颗粒。

公开(公告)号：US07764369B2

公开(公告)日：2010-07-27

申请号：US11856887

申请日：2007-09-18

Applicant: Stefan Hell ,  Volker Westphal ,  Norbert Quaas

Inventor： Stefan Hell ,  Volker Westphal ,  Norbert Quaas

IPC: G01N21/00 ,  G01J3/30

CPC classification number: G01N21/6458 ,  G01N21/6489 ,  G01N21/91 ,  G01N21/956 ,  G02B21/0076 ,  G03F7/105 ,  G03F7/70616 ,  G03F7/70675

Abstract: A method of producing spatial fine structures comprises the steps of: selecting a luminophore from the group of luminophores displaying two different states, one of the two states being an active state in which luminescence light is obtainable from the luminophore, the other of the two states being an inactive state in which no luminescence light is obtainable from the luminophore, and the luminophore being reversibly, but essentially completely, transferable out the one state into the other state by means of an optical signal; adding the luminophore to a material; forming a spatial fine structure of the material; and fluorescence-microscopically examining whether the desired fine structure is present. The step of fluorescence-microscopically examining comprises the sub-steps of: outside measuring points of interest, transferring the luminophore into the other state with the optical signal, the luminophore being essentially completely transferred into the inactive state outside the measuring points, and measuring luminescence light only emitted by the luminophore in the active state, to even resolve lines of the fine structure at a distance of less than 100 nm.

Abstract translation: 一种产生空间精细结构的方法包括以下步骤：从显示两种不同状态的发光体组中选择发光体，两种状态中的一种状态是可从发光体获得发光的活性状态，两种状态中的另一种状态 是其中从发光体不能获得发光的无活性状态，并且发光体是可逆的，但基本上完全可通过光信号转移到一个状态到另一状态; 将发光体添加到材料中; 形成材料的空间精细结构; 并进行荧光显微镜检查是否存在所需的精细结构。 荧光显微镜检查的步骤包括以下子步骤：在感兴趣的测量点之外，用光信号将发光体转移到另一状态，发光体基本上完全转移到测量点外的无效状态，并测量发光 发光体仅在活性状态下发光，甚至在小于100nm的距离处解决精细结构的线。

公开(公告)号：US07733483B2

公开(公告)日：2010-06-08

申请号：US11374287

申请日：2006-04-20

Applicant: Andreas Hecker

Inventor： Andreas Hecker

IPC: G01J3/30

CPC classification number: G01N21/6486 ,  G01N21/6458 ,  G01N21/648 ,  G02B21/16

Abstract: A method of ascertaining the orientation of molecules in a biological specimen by total internal reflection includes focusing illuminating light through an objective in different positions in a plane of a pupil of the objective so as to generate a plurality of respective differently oriented evanescent fields in the specimen. Respective different fluorescence intensities resulting from the differently oriented evanescent fields are correlated with respective different orientations of molecules in the specimen.

Abstract translation: 通过全内反射来确定生物样品中分子的取向的方法包括将目标的照明光聚焦在物镜的瞳孔平面中的不同位置，以便在样本中产生多个相应的不同取向的渐逝场 。 由不同取向的消逝场产生的各自不同的荧光强度与样品中分子的各自不同取向相关。

公开(公告)号：US20100106444A1

公开(公告)日：2010-04-29

申请号：US12388222

申请日：2009-02-18

Applicant: EDGAR GENIO ,  EDWARD W. BUDIARTO

Inventor： EDGAR GENIO ,  EDWARD W. BUDIARTO

IPC: G01J3/40 ,  G01J3/30 ,  G06F19/00

CPC classification number: G01J3/02 ,  G01J3/0205 ,  G01J3/0218 ,  G01J3/027 ,  G01J3/0291 ,  G01J3/2803 ,  G01N21/276

Abstract: In a spectrographic workpiece metrology system having an optical viewing window, the viewing window is calibrated against a reference sample of a known absolute reflectance spectrum to produce a normalized reflectance spectrum of the reference sample, which is combined with the absolute reflectance spectrum to produce a correction factor. Successive production workpieces are measured through the window and calibrated against the viewing window reflectance, and transformed to absolute reflectance spectra using the same correction factor without having to re-load the reference sample.

Abstract translation: 在具有光学观察窗口的光谱工件测量系统中，观察窗口针对已知绝对反射光谱的参考样本进行校准，以产生参考样本的归一化反射光谱，其与绝对反射光谱相结合以产生校正 因子。 通过窗口测量连续生产工件，并根据观察窗反射率进行校准，并使用相同的校正因子将其转换为绝对反射光谱，而无需重新加载参考样品。

公开(公告)号：US07701573B2

公开(公告)日：2010-04-20

申请号：US12329688

申请日：2008-12-08

Applicant: Jeffrey Cohen ,  John Maier

Inventor： Jeffrey Cohen ,  John Maier

IPC: G01J3/30

CPC classification number: G01J3/02 ,  G01J3/0264 ,  G01J3/0289 ,  G01J3/0291 ,  G01N21/6458 ,  G01N21/6486 ,  G01N21/65 ,  G01N21/658 ,  G01N2021/6417 ,  G01N2021/6423 ,  G01N2021/656

Abstract: A system and method of correlating Raman measurements with digital images of a sample so as to classify the sample's disease state. A spectroscopic data set is obtained for the sample positioned in the field of view of a spectroscopic device. With the sample removed from the field of view, the sample is treated with a contrast enhancing agent. The treated sample is repositioned in the spectroscopic device's field of view and a digital image of the treated sample is obtained. The spectroscopic data set is linked with the digital image by defining a transformation to map the image spatial coordinates of the digital image to the spectral spatial coordinates of the spectroscopic data. For the spectroscopic data set of the sample, the database is searched to identify a spectroscopic data set, of a known sample having well characterized pathology, which matches the sample's spectroscopic data set.

Abstract translation: 将拉曼测量与样本的数字图像相关联的系统和方法，以对样本的疾病状态进行分类。 对于位于分光装置的视野中的样品获得光谱数据集。 从样品中取出样品后，用对比度增强剂处理样品。 经处理的样品在分光装置的视野中重新定位，并获得处理过的样品的数字图像。 通过定义将数字图像的图像空间坐标映射到光谱数据的光谱空间坐标的变换，光谱数据集与数字图像相关联。 对于样本的光谱数据集，搜索数据库以识别具有良好表征的病理学的已知样品的光谱数据集，其与样品的光谱数据集相匹配。

公开(公告)号：US20100070197A1

公开(公告)日：2010-03-18

申请号：US12625970

申请日：2009-11-25

Applicant: Hong Wang ,  Xun Guo ,  Chunwei Liu

Inventor： Hong Wang ,  Xun Guo ,  Chunwei Liu

IPC: G01J3/44 ,  G06F19/00 ,  G01J3/30

CPC classification number: G01J3/02 ,  G01J3/0208 ,  G01J3/021 ,  G01J3/0264 ,  G01J3/44 ,  G01N21/65 ,  G01N21/658 ,  G01N21/7703 ,  G01N2021/7773 ,  G01N2201/084 ,  G01N2201/129

Abstract: A sensor-network system for spectrally sensing a chemical or biological substance includes a plurality of probe assemblies that each includes a sensor comprising a nano structured surface, wherein the nano structured surface can adsorb molecules of a sample material captured adjacent to the sensor; a laser configured to emit a laser beam to illuminate the molecules adsorbed to the nano structured surface, and a spectrometer that can obtain spectral data from light scattered by the molecules adsorbed to the nano structured surface. A control center includes a computer storage configured to store spectral signatures each associated with a chemical or biological substance and a spectral analyzer that can determine a spectral signature matching at least one of the spectral signatures stored in the computer storage thereby to identify, in the sample material, the chemical or biological substance associated with the one of the spectral signatures.

Abstract translation: 用于光谱感测化学或生物物质的传感器网络系统包括多个探针组件，每个探针组件包括包含纳米结构化表面的传感器，其中所述纳米结构化表面可以吸附邻近所述传感器捕获的样品材料的分子; 配置为发射激光束以照射吸附到纳米结构化表面的分子的激光器，以及可以由吸附到纳米结构化表面的分子散射的光获得光谱数据的光谱仪。 控制中心包括被配置为存储每个与化学或生物物质相关联的光谱特征的计算机存储器，以及光谱分析器，其可以确定与存储在计算机存储器中的至少一个光谱特征匹配的光谱签名，从而在样本中识别 材料，与光谱特征之一相关联的化学或生物物质。

公开(公告)号：US20100067003A1

公开(公告)日：2010-03-18

申请号：US12516341

申请日：2007-11-26

Applicant: Laura Marcu ,  Javier A. Jo ,  Daniel Elson

Inventor： Laura Marcu ,  Javier A. Jo ,  Daniel Elson

IPC: G01J3/30

CPC classification number: G01N21/6408 ,  G01J3/02 ,  G01J3/0205 ,  G01J3/021 ,  G01J3/0218 ,  G01J3/0294 ,  G01J3/2889 ,  G01J3/4406 ,  G01N21/6402 ,  G01N21/6486 ,  G01N2021/6421 ,  G01N2021/6484

Abstract: One embodiment of the present invention provides a system that characterizes a biological sample by analyzing light emissions from the biological sample in response to an excitation. The system first radiates the biological sample with a laser impulse to cause the biological sample to produce a responsive light emission. Next, the system uses a wavelength splitting device to split the responsive light emission into a set of spectral bands of different central wavelengths. The system applies temporal delays to the set of spectral bands so that each spectral band arrives at an optical detector at a different time, thereby allowing the optical detector to temporally resolve the responsive light emission for each spectral band separately. Next, the system captures the delayed spectral bands within a single detection window of the optical detector. The system then processes the captured spectral bands.

Abstract translation: 本发明的一个实施方案提供了一种通过响应于激发分析来自生物样品的光发射来表征生物样品的系统。 系统首先用激光脉冲辐射生物样品，使生物样品产生响应性发光。 接下来，系统使用波长分离装置将响应光发射分成不同中心波长的一组光谱带。 该系统将时间延迟应用于频谱带集合，使得每个频谱带在不同时间到达光学检测器，从而允许光学检测器分别时间地分辨每个频谱带的响应光发射。 接下来，系统捕获在光学检测器的单个检测窗口内的延迟的光谱带。 然后系统处理捕获的光谱带。

公开(公告)号：US20090305426A1

公开(公告)日：2009-12-10

申请号：US12543470

申请日：2009-08-18

Applicant: Richard REEL ,  Eric S. Nordman

Inventor： Richard REEL ,  Eric S. Nordman

IPC: G01N33/00 ,  G01J3/30

CPC classification number: G01N21/64 ,  F21V5/00 ,  G01N21/645 ,  G01N27/44721 ,  G01N2021/6463 ,  G01N2201/068 ,  Y10T436/143333

Abstract: System and method for fluorescent light excitation and detection from samples to enhance the numerical aperture and/or reduce the cross-talk of the fluorescent light.

Abstract translation: 用于荧光灯激发和检测样品的系统和方法，以提高数值孔径和/或减少荧光灯的串扰。

公开(公告)号：US20090303474A1

公开(公告)日：2009-12-10

申请号：US12543904

申请日：2009-08-19

Applicant: Marcus Dyba ,  Hilmar Gugel

Inventor： Marcus Dyba ,  Hilmar Gugel

IPC: G01J3/30

CPC classification number: G01N21/6458 ,  G02B21/00 ,  G02B21/16

Abstract: A method and a microscope, in particular a laser scanning fluorescence microscope, for high spatial resolution examination of samples, the sample (1) to be examined comprising a substance that can be repeatedly converted from a first state (Z1, A) into a second state (Z2, B), the first and the second states (Z1, A; Z2, B) differing from one another in at least one optical property, comprising the steps that the substance in a sample region (P) to be recorded is firstly brought into the first state (Z1, A), and that the second state (Z2, B) is induced by means of an optical signal (4), spatially delimited subregions being specifically excluded within the sample region (P) to be recorded, are defined in that the optical signal (4) is provided in such a way that a standing wave with defined intensity zero points (5) is formed in the sample region (P) to be recorded.

Abstract translation: 一种用于样品的高空间分辨率检查的方法和显微镜，特别是激光扫描荧光显微镜，待检查的样品（1）包括可以从第一状态（Z1，A）重复转换成第二状态 状态（Z2，B），在至少一个光学特性中彼此不同的第一和第二状态（Z1，A; Z2，B）包括以下步骤：待记录的样本区域（P）中的物质是 首先进入第一状态（Z1，A），并且通过光信号（4）感应第二状态（Z2，B），在要记录的采样区域（P）内特别排除空间划分的子区域 定义为光信号（4）的设置方式是在要记录的样本区域（P）中形成具有限定强度零点（5）的驻波。