公开(公告)号：US20180328934A1

公开(公告)日：2018-11-15

申请号：US15779944

申请日：2016-11-29

Applicant: DH Technologies Development Pte. Ltd.

Inventor： John L. Campbell ,  Yves LeBlanc ,  Chang Liu

IPC: G01N33/58 ,  G01N27/62 ,  G01N33/68 ,  H01J49/00

CPC classification number: G01N33/582 ,  G01N27/624 ,  G01N33/58 ,  G01N33/6848 ,  H01J49/0045

Abstract: Methods and systems for separating and/or quantifying compounds, using differential mobility spectrometry (DMS) are provided herein. In accordance with various aspects of the applicants' teachings, the methods and systems can provide for the quantification of one or more compounds, for example, using isomeric labels that can be less costly to produce relative to conventional tags that incorporate stable-isotope labels. The present teachings can quantify the relative amount of a compound based on the effect of using an easily charged functional group as well as a functional group positioned at a resonant or non-resonant position through a DMS. In some aspects, methods and systems in accordance with various aspects of the present teachings provide for the detection and/or quantification of the analytes labeled with isomeric tags that can be differentiated via a DMS upstream of a first stage mass analyzer and/or prior to fragmentation of the labeled analyte, for example.

公开(公告)号：US20170074871A1

公开(公告)日：2017-03-16

申请号：US15121803

申请日：2015-02-27

Applicant: DH Technologies Development PTE Ltd.

Inventor： John L. Campbell ,  Thomas R. Covey ,  Chang Liu ,  Subhasish Purkayastha

IPC: G01N33/543 ,  B01F13/08 ,  B01F13/00 ,  B01L3/00 ,  G01N1/38

CPC classification number: G01N33/54326 ,  B01F13/0059 ,  B01F13/0818 ,  B01L3/50273 ,  B01L3/502792 ,  B01L2200/0647 ,  B01L2300/0819 ,  B01L2400/0427 ,  B01L2400/043 ,  G01N1/38 ,  G01N27/745 ,  G01N35/0098 ,  G01N2001/386

Abstract: Methods and apparatus for processing fluids on a macro- or micro-scale are described. In various aspects, a fluid may have a plurality of elongated (i.e., substantially rod-shaped) magnetic elements disposed therein within a fluid container. An illustrative fluid container is an actuator electrode or a processing vial of a microfluidic device, such as a digital microfluidic device. A magnet component may be configured to generate a magnetic force sufficient to influence the movement of the plurality of elongated magnetic elements within the fluid to be processed. For example, the magnetic force (or magnetic force gradient) may influence the plurality of elongated magnetic elements to rotate, spin, and/or move laterally side-to-side. The shape and movements of the plurality of elongated magnetic elements facilitate the rapid and efficient processing of the fluid, such as fluid mixing and/or fluid separation.

Abstract translation: 描述了在宏观或微观尺度上处理流体的方法和装置。 在各个方面，流体可以具有设置在流体容器内的多个细长（即基本棒状）的磁性元件。 说明性的流体容器是诸如数字微流体装置的微流体装置的致动器电极或处理小瓶。 磁体部件可以被配置为产生足以影响待处理流体中的多个细长磁性元件的运动的磁力。 例如，磁力（或磁力梯度）可以影响多个细长的磁性元件以使它们在侧面上相互旋转，旋转和/或横向移动。 多个细长磁性元件的形状和运动有助于流体的快速和有效的处理，例如流体混合和/或流体分离。

公开(公告)号：US09196468B2

公开(公告)日：2015-11-24

申请号：US14395705

申请日：2013-05-17

Applicant: DH Technologies Development Pte. Ltd.

Inventor： John L. Campbell ,  Thomas Covey ,  Yves LeBlanc ,  Bradley B. Schneider

IPC: B05B5/025 ,  G01N30/02 ,  H01J49/00 ,  F23D11/22 ,  H01J49/16 ,  G01N27/62 ,  H01J49/04

CPC classification number: H01J49/165 ,  G01N27/622 ,  H01J49/0081 ,  H01J49/0431

Abstract: An electrospray ion source method and system is provided for detecting emitter failure comprising a liquid chromatography column suitable for chromatographic separation of a sample. The column can have an inlet for receiving the sample; and an outlet for ejecting the sample. A make-up flow channel is provided for introducing make-up flow of liquid to the sample post-column, wherein the make-up flow normalizes the spray current. An electrospray ionization source is provided having one or more electrospray ionization emitter nozzles for receiving the make-up flow containing sample. A power supply can provide a voltage to the one or more emitter nozzles, and a measurement device can measure and monitor the spray current.

Abstract translation: 提供了一种用于检测发射器故障的电喷雾离子源方法和系统，其包括适于样品色谱分离的液相色谱柱。 该柱可以具有用于接收样品的入口; 以及用于喷射样品的出口。 提供了补充流动通道，用于将液体的补充流引入样品后柱，其中补充流使归一化喷雾电流。 提供了具有一个或多个电喷雾电离发射器喷嘴的电喷雾离子源，用于接收含有补充流的样品。 电源可以向一个或多个发射器喷嘴提供电压，并且测量装置可以测量和监测喷射电流。

公开(公告)号：US20180108521A1

公开(公告)日：2018-04-19

申请号：US15556698

申请日：2016-03-08

Applicant: DH Technologies Development Pte Ltd.

Inventor： John L. Campbell

IPC: H01J49/00 ,  G01N30/72 ,  G01N30/86 ,  H01J49/42

CPC classification number: H01J49/0081 ,  G01N30/7233 ,  G01N30/8631 ,  G01N30/8644 ,  G01N30/8689 ,  H01J49/0027 ,  H01J49/0036 ,  H01J49/004 ,  H01J49/4265

Abstract: A method and apparatus for improving the quality of spectra of a sample obtained from a tandem mass spectrometer system containing an ion trap. The method and apparatus includes the setting of an upper and lower threshold limit on peak intensity and only triggering an enhanced product ion scan when a detected intensity of a peak in an initial scan falls between the upper and lower threshold limits. The spectra obtained from an enhanced product ion scan conducted in this manner are useful in library matching of spectra. The ion trap may be a linear ion trap and the sample may be a peptide.

公开(公告)号：US09360455B2

公开(公告)日：2016-06-07

申请号：US14655263

申请日：2013-12-13

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： John L. Campbell ,  Yves Leblanc

IPC: G01N33/92 ,  G01N27/62 ,  H01J49/00 ,  H01J49/04

CPC classification number: G01N27/624 ,  G01N27/622 ,  G01N33/92 ,  G01N2458/15 ,  H01J49/0031 ,  H01J49/0422 ,  Y10T436/24

Abstract: A method for analyzing a sample that contains a plurality of lipid isomers is described that involves forming one or more lipid metal ion adducts and transporting the one or more lipid metal ion adducts through a differential mobility spectrometer to cause separation of the one or more lipid metal ion adducts from each other. The lipid isomers can be chosen, for example, from fatty acids, glycerolipids, glycerophospholipids, sphingolipids, saccharolipids, polyketides, sterol lipids, and prenol lipids. Particular examples include phosphatidylcholine regioisomers such as 1-palmatoyl-2-oleoyl-sn-phosphatidylcholine (POPC) and 1-oleoyl-2-palmatoyl-sn-phosphatidylcholine and triacylglycerols containing palmetic and oleic acid groups. The metal chosen can include a cationization reagent that contains sodium, potassium, silver or lithium.

Abstract translation: 描述了分析含有多种脂质异构体的样品的方法，其涉及形成一种或多种脂质金属离子加合物并将一种或多种脂质金属离子加合物输送通过差速迁移谱仪，以引起一种或多种脂质金属 离子加合物彼此。 脂质异构体可以选自例如脂肪酸，甘油脂，甘油磷脂，鞘脂，蔗糖脂，聚酮化合物，甾醇脂质和牛油醇脂质。 特别的实例包括磷脂酰胆碱区域异构体如1-棕榈酰-2-油酰-sn-磷脂酰胆碱（POPC）和1-油酰-2-棕榈酰-sn-磷脂酰胆碱和含有油酸和油酸基团的三酰基甘油。 所选择的金属可以包括含有钠，钾，银或锂的阳离子化试剂。

公开(公告)号：US20150076342A1

公开(公告)日：2015-03-19

申请号：US14395705

申请日：2013-05-17

Applicant: DH Technologies Development Pte. Ltd.

Inventor： John L. Campbell ,  Thomas Covey ,  Yves LeBlanc ,  Bradley B. Schneider

IPC: H01J49/16 ,  G01N27/62 ,  H01J49/00 ,  H01J49/04

CPC classification number: H01J49/165 ,  G01N27/622 ,  H01J49/0081 ,  H01J49/0431

Abstract: An electrospray ion source method and system is provided for detecting emitter failure comprising a liquid chromatography column suitable for chromatographic separation of a sample. The column can have an inlet for receiving the sample; and an outlet for ejecting the sample. A make-up flow channel is provided for introducing make-up flow of liquid to the sample post-column, wherein the make-up flow normalizes the spray current. An electrospray ionization source is provided having one or more electrospray ionization emitter nozzles for receiving the make-up flow containing sample. A power supply can provide a voltage to the one or more emitter nozzles, and a measurement device can measure and monitor the spray current.

Abstract translation: 提供了一种用于检测发射器故障的电喷雾离子源方法和系统，其包括适于样品色谱分离的液相色谱柱。 该柱可以具有用于接收样品的入口; 以及用于喷射样品的出口。 提供了补充流动通道，用于将液体的补充流引入样品后柱，其中补充流使标准化喷雾电流。 提供了具有一个或多个电喷雾电离发射器喷嘴的电喷雾离子源，用于接收含有补充流的样品。 电源可以向一个或多个发射器喷嘴提供电压，并且测量装置可以测量和监测喷射电流。

公开(公告)号：US11085925B2

公开(公告)日：2021-08-10

申请号：US15779944

申请日：2016-11-29

Applicant: DH Technologies Development Pte. Ltd.

Inventor： John L. Campbell ,  Yves LeBlanc ,  Chang Liu

IPC: G01N33/00 ,  G01N33/58 ,  G01N27/624 ,  G01N33/68 ,  H01J49/00

Abstract: Methods and systems for separating and/or quantifying compounds, using differential mobility spectrometry (DMS) are provided herein. In accordance with various aspects of the applicants' teachings, the methods and systems can provide for the quantification of one or more compounds, for example, using isomeric labels that can be less costly to produce relative to conventional tags that incorporate stable-isotope labels. The present teachings can quantify the relative amount of a compound based on the effect of using an easily charged functional group as well as a functional group positioned at a resonant or non-resonant position through a DMS. In some aspects, methods and systems in accordance with various aspects of the present teachings provide for the detection and/or quantification of the analytes labeled with isomeric tags that can be differentiated via a DMS upstream of a first stage mass analyzer and/or prior to fragmentation of the labeled analyte, for example.

公开(公告)号：US10656147B2

公开(公告)日：2020-05-19

申请号：US15121803

申请日：2015-02-27

Applicant: DH Technologies Development PTE Ltd.

Inventor： John L. Campbell ,  Thomas R. Covey ,  Chang Liu ,  Subhasish Purkayastha

IPC: G01N33/553 ,  G01N33/543 ,  G01N1/38 ,  B01F13/00 ,  B01F13/08 ,  G01N35/00 ,  B01L3/00 ,  G01N27/74

Abstract: Methods and apparatus for processing fluids on a macro- or micro-scale are described. In various aspects, a fluid may have a plurality of elongated (i.e., substantially rod-shaped) magnetic elements disposed therein within a fluid container. An illustrative fluid container is an actuator electrode or a processing vial of a microfluidic device, such as a digital microfluidic device. A magnet component may be configured to generate a magnetic force sufficient to influence the movement of the plurality of elongated magnetic elements within the fluid to be processed. For example, the magnetic force (or magnetic force gradient) may influence the plurality of elongated magnetic elements to rotate, spin, and/or move laterally side-to-side. The shape and movements of the plurality of elongated magnetic elements facilitate the rapid and efficient processing of the fluid, such as fluid mixing and/or fluid separation.

公开(公告)号：US20180369831A1

公开(公告)日：2018-12-27

申请号：US15779933

申请日：2016-11-29

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Don W. Arnold ,  Thomas R. Covey ,  Yves LeBlanc ,  Chang Liu ,  John L. Campbell

IPC: B03C1/28 ,  B03C1/01 ,  B03C1/033 ,  G01N1/38

Abstract: Methods and apparatus for processing fluids are described. In various aspects, a fluid processing system may include a magnetic assembly that includes a plurality of magnetic structures configured to generate a magnetic field gradient within a fluid container. The magnetic structures may be formed as a plurality of electromagnets configured to be individually actuated by a controller. Each of the electromagnets may generate a magnetic field within the fluid container. The electromagnets may be differentially actuated to create a magnetic field gradient within the fluid container to agitate, mix, or otherwise influence magnetic particles disposed within the fluid container. Activation of the electromagnets of an electromagnetic structure may generate a magnetic field gradient that influences magnetic particles in an x-y direction. In addition, activation of the electromagnets of a plurality of electromagnetic structures may generate magnetic field gradients that influences magnetic particles in an x-y direction and z-direction.