公开(公告)号：US10001456B2

公开(公告)日：2018-06-19

申请号：US15511712

申请日：2015-08-28

Applicant: DH Technologies Development PTE Ltd.

Inventor： John L Campbell ,  Yves LeBlanc ,  Chang Liu

IPC: C07K1/14 ,  C07K1/00 ,  B01D59/44 ,  B01D15/38 ,  B01D15/36 ,  C12Q1/68 ,  G01N33/53 ,  H01J49/26 ,  G01N27/62 ,  H01J49/04 ,  H01J49/10

CPC classification number: G01N27/624 ,  G01N33/6803 ,  H01J49/04 ,  H01J49/10

Abstract: Methods and systems for separating and/or quantifying peptides using differential mobility spectrometry (DMS) are provided herein. In accordance with various aspects of the applicant's teachings, the methods and systems can provide for the separation of one or more peptides, for example, peptides that may be difficult to separate with conventional techniques, such as mass spectrometry (MS), by complexing the peptides with a metal cation (e.g., Ca2+) prior to DMS. In some aspects, the present teachings can prevent proton stripping from ionized peptides that can occur in DMS to prevent unintended and/or undesirable alterations to the peptide's charge state distribution.

公开(公告)号：US09134273B2

公开(公告)日：2015-09-15

申请号：US14397236

申请日：2013-05-17

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Yves LeBlanc

IPC: G01N33/50 ,  B01D59/44 ,  G01N27/62 ,  H01J49/00

CPC classification number: G01N27/622 ,  G01N27/624 ,  G01N33/50 ,  G01N2800/02 ,  H01J49/00

Abstract: Methods and systems for performing ion mobility spectrometry are provided herein. In accordance with various aspects of the applicant's teachings, the methods and systems can provide for the separation of biologically relevant acids that may be difficult to separate with conventional MS techniques. In various aspects, methods and systems in accordance with applicant's teachings can enable a differential mobility spectrometer to resolve biologically relevant acids through the use of CO2 as the drift gas in combination with Acetone.

Abstract translation: 本文提供了用于进行离子迁移光谱法的方法和系统。 根据申请人的教导的各个方面，所述方法和系统可以提供与常规MS技术难以分离的生物相关酸的分离。 在各个方面，根据申请人的教导的方法和系统可以使差示迁移谱仪能够通过使用CO 2作为与丙酮组合的漂移气体来分解生物相关的酸。

公开(公告)号：US20150076342A1

公开(公告)日：2015-03-19

申请号：US14395705

申请日：2013-05-17

Applicant: DH Technologies Development Pte. Ltd.

Inventor： John L. Campbell ,  Thomas Covey ,  Yves LeBlanc ,  Bradley B. Schneider

IPC: H01J49/16 ,  G01N27/62 ,  H01J49/00 ,  H01J49/04

CPC classification number: H01J49/165 ,  G01N27/622 ,  H01J49/0081 ,  H01J49/0431

Abstract: An electrospray ion source method and system is provided for detecting emitter failure comprising a liquid chromatography column suitable for chromatographic separation of a sample. The column can have an inlet for receiving the sample; and an outlet for ejecting the sample. A make-up flow channel is provided for introducing make-up flow of liquid to the sample post-column, wherein the make-up flow normalizes the spray current. An electrospray ionization source is provided having one or more electrospray ionization emitter nozzles for receiving the make-up flow containing sample. A power supply can provide a voltage to the one or more emitter nozzles, and a measurement device can measure and monitor the spray current.

Abstract translation: 提供了一种用于检测发射器故障的电喷雾离子源方法和系统，其包括适于样品色谱分离的液相色谱柱。 该柱可以具有用于接收样品的入口; 以及用于喷射样品的出口。 提供了补充流动通道，用于将液体的补充流引入样品后柱，其中补充流使标准化喷雾电流。 提供了具有一个或多个电喷雾电离发射器喷嘴的电喷雾离子源，用于接收含有补充流的样品。 电源可以向一个或多个发射器喷嘴提供电压，并且测量装置可以测量和监测喷射电流。

公开(公告)号：US20140264009A1

公开(公告)日：2014-09-18

申请号：US14361378

申请日：2012-11-21

Applicant: DH Technologies Development Pte. Ltd.

Inventor： John Lawrence Campbell ,  Yves LeBlanc

IPC: G01N27/62

CPC classification number: G01N27/624 ,  G01N27/622 ,  G01N33/6848 ,  H01J49/0072

Abstract: An apparatus and method are provided for analyzing samples of molecules. The apparatus comprises a mass analysis system including a differential mobility spectrometer, which includes at least three filter electrodes defining two ion flow paths where the filter electrodes generate electric fields for passing through selected portions of the sample ions based on the mobility characteristics of the sample ions. The differential mobility spectrometer also includes a voltage source that provides DC and RF voltages to at least one of the filter electrodes to generate the electric field, a first and a second ion inlet that receive sample ions, and an ion outlet that outputs the selected portion of the sample ions. A mass spectrometer receives some or all of the selected portion of the sample ions.

Abstract translation: 提供了用于分析分子样品的装置和方法。 该装置包括质量分析系统，其包括差动迁移谱仪，其包括限定两个离子流动路径的至少三个过滤器电极，其中过滤电极基于样品离子的迁移率特性产生用于穿过样本离子的选定部分的电场 。 差动迁移谱仪还包括电压源，其向至少一个滤波电极提供DC和RF电压以产生电场;接收样品离子的第一和第二离子入口;以及输出所选部分的离子出口 的样品离子。 质谱仪接收样品离子的部分或全部选定部分。

公开(公告)号：US09299549B2

公开(公告)日：2016-03-29

申请号：US14367236

申请日：2012-12-07

Applicant: DH Technologies Development Pte. Ltd.

Inventor： John Lawrence Campbell ,  Yves LeBlanc

IPC: H01J49/00 ,  H01J49/10

CPC classification number: H01J49/0072 ,  H01J49/0077 ,  H01J49/10 ,  H01J49/107

Abstract: An apparatus, system and method of providing that allow for the generation of reagent ions within an inner region of a mass spectrometer for use in ion-ion reactions such as PTRs and ETD using a reagent ion generator. The location where these reagent ions are generated can be as close as possible to the point of action, or the reaction zone where the reagent ion and analyte ions will interact via ion-ion reactions to cause, e.g., PTRs and/or ETD.

Abstract translation: 一种提供允许在质谱仪的内部区域内产生试剂离子以用于离子离子反应如PTR和ETD的试剂离子发生器的装置，系统和方法。 产生这些试剂离子的位置可以尽可能接近作用点，或反应区，其中试剂离子和分析物离子将通过离子 - 离子反应相互作用以引起例如PTR和/或ETD。

公开(公告)号：US11085925B2

公开(公告)日：2021-08-10

申请号：US15779944

申请日：2016-11-29

Applicant: DH Technologies Development Pte. Ltd.

Inventor： John L. Campbell ,  Yves LeBlanc ,  Chang Liu

IPC: G01N33/00 ,  G01N33/58 ,  G01N27/624 ,  G01N33/68 ,  H01J49/00

Abstract: Methods and systems for separating and/or quantifying compounds, using differential mobility spectrometry (DMS) are provided herein. In accordance with various aspects of the applicants' teachings, the methods and systems can provide for the quantification of one or more compounds, for example, using isomeric labels that can be less costly to produce relative to conventional tags that incorporate stable-isotope labels. The present teachings can quantify the relative amount of a compound based on the effect of using an easily charged functional group as well as a functional group positioned at a resonant or non-resonant position through a DMS. In some aspects, methods and systems in accordance with various aspects of the present teachings provide for the detection and/or quantification of the analytes labeled with isomeric tags that can be differentiated via a DMS upstream of a first stage mass analyzer and/or prior to fragmentation of the labeled analyte, for example.

公开(公告)号：US11031225B2

公开(公告)日：2021-06-08

申请号：US16335077

申请日：2017-09-13

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Thomas R. Covey ,  Yves LeBlanc ,  Bradley B. Schneider

IPC: H01J49/06 ,  G01N30/72 ,  G01N30/84

Abstract: Because most ion optics of mass spectrometry systems are subject to ion deposition and may exhibit significantly different behavior following substantial contamination (e.g., loss of sensitivity), fouled surfaces must be regularly cleaned to maintain sensitivity. While the surfaces of front-end components (e.g., curtain plates, orifice plates, Qjet, Q0, IQ0) may be relatively easy to clean, the fouling of components contained within the downstream high-vacuum chambers (e.g., Q1, IQ1) can incur substantial delays and expense as the high-vacuum chambers must be vented and substantially disassembled prior to cleaning. Methods and systems for controlling contamination of components of mass spectrometer systems are provided herein. By reducing the transmission of contaminating ions during non-data acquisition periods, the present teachings can increase throughput, improve robustness, and/or decrease the downtime typically required to vent/disassemble/clean the fouled components.

公开(公告)号：US20180369831A1

公开(公告)日：2018-12-27

申请号：US15779933

申请日：2016-11-29

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Don W. Arnold ,  Thomas R. Covey ,  Yves LeBlanc ,  Chang Liu ,  John L. Campbell

IPC: B03C1/28 ,  B03C1/01 ,  B03C1/033 ,  G01N1/38

Abstract: Methods and apparatus for processing fluids are described. In various aspects, a fluid processing system may include a magnetic assembly that includes a plurality of magnetic structures configured to generate a magnetic field gradient within a fluid container. The magnetic structures may be formed as a plurality of electromagnets configured to be individually actuated by a controller. Each of the electromagnets may generate a magnetic field within the fluid container. The electromagnets may be differentially actuated to create a magnetic field gradient within the fluid container to agitate, mix, or otherwise influence magnetic particles disposed within the fluid container. Activation of the electromagnets of an electromagnetic structure may generate a magnetic field gradient that influences magnetic particles in an x-y direction. In addition, activation of the electromagnets of a plurality of electromagnetic structures may generate magnetic field gradients that influences magnetic particles in an x-y direction and z-direction.

公开(公告)号：US20150129762A1

公开(公告)日：2015-05-14

申请号：US14401399

申请日：2013-05-17

Applicant: DH Technologies Development Pte. Ltd.

Inventor： John Lawrence Campbell ,  Yves LeBlanc

IPC: G01N27/62 ,  H01J49/00

CPC classification number: G01N27/624 ,  H01J49/0031 ,  H01J49/0431

Abstract: Methods and systems for performing mass spectrometry of analytes labeled with isobaric tags are provided herein. In accordance with various aspects of the applicants' teachings, the methods and systems can enable enhanced discrimination between an analyte of interest and one or more interfering species when using isobaric tagging techniques.

Abstract translation: 本文提供了用等压标签标记的分析物进行质谱分析的方法和系统。 根据申请人的教导的各个方面，当使用等压标签技术时，方法和系统可以增强感兴趣的分析物与一种或多种干扰物质之间的增强的区分。

公开(公告)号：US20190221415A1

公开(公告)日：2019-07-18

申请号：US16335077

申请日：2017-09-13

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Thomas R. Covey ,  Yves LeBlanc ,  Bradley B. Schneider

IPC: H01J49/06 ,  G01N30/84 ,  G01N30/72

CPC classification number: H01J49/067 ,  G01N30/7233 ,  G01N30/84

Abstract: Because most ion optics of mass spectrometry systems are subject to ion deposition and may exhibit significantly different behavior following substantial contamination (e.g., loss of sensitivity), fouled surfaces must be regularly cleaned to maintain sensitivity. While the surfaces of front-end components (e.g., curtain plates, orifice plates, Qjet, Q0, IQ0) may be relatively easy to clean, the fouling of components contained within the downstream high-vacuum chambers (e.g., Q1, IQ1) can incur substantial delays and expense as the high-vacuum chambers must be vented and substantially disassembled prior to cleaning. Methods and systems for controlling contamination of components of mass spectrometer systems are provided herein. By reducing the transmission of contaminating ions during non-data acquisition periods, the present teachings can increase throughput, improve robustness, and/or decrease the downtime typically required to vent/disassemble/clean the fouled components.