公开(公告)号：US08326404B2

公开(公告)日：2012-12-04

申请号：US10596072

申请日：2004-11-26

Applicant: Haishan Zeng ,  Harvey Lui ,  Zhiwei Huang ,  David I. McLean

Inventor： Haishan Zeng ,  Harvey Lui ,  Zhiwei Huang ,  David I. McLean

IPC: A61B6/00

CPC classification number: A61B5/0071 ,  A61B5/0035 ,  A61B5/0075 ,  A61B5/0086 ,  A61B5/444 ,  A61B5/7264 ,  G01N21/6486 ,  G01N21/65

Abstract: Methods and apparatus for classifying tissue use features of Raman spectra and background fluorescent spectra. The spectra may be acquired in the near-infrared wavelengths. Principal component analysis and linear discriminant analysis of reference spectra may be used to obtain a classification function that accepts features of the Raman and background fluorescence spectra for test tissue and yields an indication as to the likelihood that the test tissue is abnormal. The methods and apparatus may be applied to screening for skin cancers or other diseases.

Abstract translation: 用于分类拉曼光谱和背景荧光光谱的组织使用特征的方法和装置。 可以在近红外波长中获取光谱。 可以使用参考光谱的主成分分析和线性判别分析来获得接受测试组织的拉曼特征和背景荧光光谱的分类函数，并产生关于测试组织异常的可能性的指示。 所述方法和装置可用于筛选皮肤癌或其它疾病。

公开(公告)号：US07697975B2

公开(公告)日：2010-04-13

申请号：US10453040

申请日：2003-06-03

Applicant: Haishan Zeng

Inventor： Haishan Zeng

IPC: A61B6/00

CPC classification number: G01N21/6486 ,  A61B1/042 ,  A61B1/043 ,  A61B5/0071 ,  A61B5/0084 ,  G01N15/1468 ,  G01N21/6456 ,  G01N2021/6419 ,  G01N2021/6421

Abstract: Methods and apparati for fluorescence imaging using multiple excitation-emission pairs is revealed. A target object is illuminated by light in at least two spectral regions, causing fluorescence emission in at least two spectral regions. The emitted light is collected and separated for analysis.

Abstract translation: 揭示了使用多个激发 - 发射对的荧光成像的方法和装置。 目标物体被至少两个光谱区域的光照亮，从而在至少两个光谱区域中产生荧光发射。 发射的光被收集和分离用于分析。

公开(公告)号：US06486948B1

公开(公告)日：2002-11-26

申请号：US09659300

申请日：2000-09-12

Applicant: Haishan Zeng

Inventor： Haishan Zeng

IPC: G01J344

CPC classification number: G01J3/1838 ,  G01J3/0221 ,  G01J3/04 ,  G01J3/44 ,  G01J2003/2866

Abstract: Systems and methods for rapid Raman spectroscopy. The speed is improved by providing light from a sample to a light-dispersive element, such as a holographic grating, in a pattern that inversely complements distortion caused by the grating. For example, if the grating imparts a curve to the spectral lines emanating from the grating, then the light is inserted into the grating in a curve in the opposite direction. Also calibration light guides able to transmit a known, or standard, light to the detection or spectroscopy system. The calibration light guide can be useful both with traditional light transmission guides and with the light transmission guides of the present invention.

Abstract translation: 快速拉曼光谱的系统和方法。 通过将样品中的光提供给诸如全息光栅的光分散元件，以与由光栅引起的失真相反的图案来提高速度。 例如，如果光栅对从光栅发出的光谱线施加曲线，则将光以相反方向的曲线插入光栅。 还有校准光导能够将已知或标准的光传输到检测或光谱系统。 校准光导可以与传统的光传输引导件和本发明的光传输引导件一起使用。

公开(公告)号：US06269818B1

公开(公告)日：2001-08-07

申请号：US09084865

申请日：1998-05-26

Applicant: Harvey Lui ,  Calum Macaulay ,  Haishan Zeng ,  David I. McLean ,  Robert Bissonnette

Inventor： Harvey Lui ,  Calum Macaulay ,  Haishan Zeng ,  David I. McLean ,  Robert Bissonnette

IPC: A61B1900

CPC classification number: A61K49/0036 ,  A61K41/0071 ,  A61K49/0017 ,  A61N5/062

Abstract: In one aspect, the invention provides a diagnostic method for identifying psoriatic plaques in which Porphyrins, particularly protoporphyrin IX, are elevated as compared to normal skin and skin of patients with other dermatological diseases, including other forms of psoriatic plaque. Psoriatic plaques with elevated porphyrin levels may be detected by fluorescence and spectral analysis. Endogenous porphyrins in psoriatic plaques may be activated with visible light to treat psoriatic plaques having elevated porphyrin concentrations. Skin conditions may be optimized to increase the endogenous concentration of porphyrins in psoriatic plaques. A topical formulation may be applied to psoriatic plaques to optimize skin conditions such as pH, iron concentration, temperature, hydration, calcium concentration, oxygenation, electrical conductivity and estrogen concentration to increase the concentration of endogenous porphyrins.

Abstract translation: 一方面，本发明提供了一种鉴别银屑病斑块的诊断方法，其中卟啉，特别是原卟啉IX，与具有其他皮肤病学疾病，包括其他形式的银屑病斑块的患者的正常皮肤和皮肤相比升高。 通过荧光和光谱分析可以检测到卟啉水平升高的银屑病斑块。 银屑病斑块中的内源性卟啉可以用可见光激活以治疗具有升高的卟啉浓度的银屑病斑块。 可以优化皮肤状况以增加银屑病斑块中卟啉的内源性浓度。 局部制剂可以应用于银屑病斑块以优化皮肤状况，例如pH，铁浓度，温度，水合，钙浓度，氧合，电导率和雌激素浓度以增加内源卟啉的浓度。

公开(公告)号：US6021344A

公开(公告)日：2000-02-01

申请号：US984391

申请日：1997-12-03

Applicant: Harvey Lui ,  Haishan Zeng ,  Calum E. MacAulay ,  Branko Palcic ,  David I. McLean

Inventor： Harvey Lui ,  Haishan Zeng ,  Calum E. MacAulay ,  Branko Palcic ,  David I. McLean

IPC: A61B5/00 ,  A61B5/103 ,  A61B10/00

CPC classification number: A61B5/444 ,  A61B5/0059 ,  A61B5/0071 ,  A61B2090/502

Abstract: Apparatus for the diagnosis of a skin disease site by visual fluorescence inspection comprising an excitation light source for illuminating the disease site, a light guide for transmitting the excitation light directly to the disease site to generate fluorescence light and viewing goggles for processing the excitation light reflected and the fluorescence light emitted from the disease site to provide a fluorescence image of the disease site to a user. The fluorescence image is used to aid the medical assessment of skin conditions and the diagnosis of cutaneous diseases by supplementing the visual assessment of skin lesions made by the naked eye. The apparatus can be used in several modes of operation that permit the viewing of full color fluorescence images and enhanced two color images. The apparatus can also use image intensifying equipment to amplify fluorescence light so that even very weak fluorescing objects can be seen. A method for acquiring and viewing the fluorescence images is also disclosed.

Abstract translation: 用于通过视觉荧光检查诊断皮肤疾病部位的装置，包括用于照射疾病部位的激发光源，用于将激发光直接传播到疾病部位以产生荧光的光导和观察用于处理反射的激发光的护目镜 以及从疾病部位发出的荧光，向用户提供疾病部位的荧光图像。 荧光图像用于通过补充肉眼皮肤损伤的视觉评估来帮助对皮肤状况的医学评估和皮肤疾病的诊断。 该装置可以用于允许观看全色荧光图像和增强的双色图像的几种操作模式。 该装置还可以使用图像增强设备来放大荧光，使得甚至可以看到非常弱的荧光物体。 还公开了一种用于获取和观察荧光图像的方法。

公开(公告)号：US20120259229A1

公开(公告)日：2012-10-11

申请号：US13516715

申请日：2010-12-17

Applicant: Hequn Wang ,  Haishan Zeng ,  Harvey Lui ,  Jianhua Zhao

Inventor： Hequn Wang ,  Haishan Zeng ,  Harvey Lui ,  Jianhua Zhao

IPC: A61B6/00

CPC classification number: G01N21/65 ,  A61B5/0075 ,  A61B5/444

Abstract: A micro-Raman spectrometer system for use in differentiating tumor lesions from normal skin detects specific characteristics of Raman spectra indicative of cancer. A peak at 899 cm−1 and a higher intensity region in the 1325 cm−1 to 1330 cm−1 range indicate the presence of tumors. The spectrometer system may be applied for skin cancer detection and for mapping the margins of lesions. Cancer detection methods as described herein have achieved diagnostic sensitivity of 95.8% and specificity of 93.8%.

Abstract translation: 用于区分肿瘤病变与正常皮肤的微拉曼光谱仪系统检测指示癌症的拉曼光谱的特征。 在899cm-1处的峰和1325cm-1至1330cm-1范围内的较高强度区域表示存在肿瘤。 光谱仪系统可以应用于皮肤癌检测和用于映射病变的边缘。 本文所述的癌症检测方法具有95.8％的诊断敏感性和93.8％的特异性。

公开(公告)号：US20120089030A1

公开(公告)日：2012-04-12

申请号：US13144574

申请日：2010-01-18

Applicant: Kevin Guze ,  Michael Short ,  Haishan Zeng ,  Stephen Sonis

Inventor： Kevin Guze ,  Michael Short ,  Haishan Zeng ,  Stephen Sonis

IPC: A61B6/00

CPC classification number: G01N21/65 ,  A61B5/0088 ,  A61B5/682 ,  G01N2021/656

Abstract: A method and system for characterizing tissue includes a probe connected to a red LASER source and a Raman spectroscope. The probe includes at least excitation fiber and one or more emission fibers that connect the probe with the LASER source and the Raman spectroscope. The excitation fiber is connected to the red LASER source and terminates in the first end of the probe adjacent the tip of the probe. The emission fibers are connected to the Raman spectroscope and terminate in the first end of the probe adjacent the tip of the probe. In one embodiment, the excitation fiber extends through the central portion of the probe and one or more emission fibers are arranged around the excitation fiber. The tip of the probe is intended to come in contact with the tissue to be examined. The tip includes a central opening to allow red LASER radiation to project out of the end of the red excitation fiber on to the tissue and to permit Raman spectra to enter the emission fiber(s) and travel to the Raman spectroscope. The tip is constructed to have a predefined focal length to position the first end of the probe a predefined distance from the surface of the tissue being examined. The tip can be removable and tips having different focal lengths can be used to accommodate different types of tissues and examinations. A detector can convert the Raman spectra into signals and data for analysis by a computer system. The Raman spectra for tissue in a predefined location can be profiled such that the system can distinguish between healthy and diseased tissue.

Abstract translation: 用于表征组织的方法和系统包括连接到红色激光源和拉曼光谱仪的探针。 该探针至少包括激发光纤和将探头与激光源和拉曼光谱仪连接的一个或多个发射光纤。 激发光纤连接到红色激光源，并且终止于探头的第一端附近的探针尖端。 发射光纤连接到拉曼光谱仪并终止在探头的第一端附近探针的尖端。 在一个实施例中，激发光纤延伸穿过探针的中心部分，并且一个或多个发射光纤布置在激发光纤周围。 探针的尖端旨在与要检查的组织接触。 尖端包括中心开口，以允许红色激光辐射从红色激发光纤的末端突出到组织上，并允许拉曼光谱进入发射光纤并行进到拉曼光谱仪。 尖端被构造成具有预定的焦距，以将探针的第一端定位在距待检查组织的表面的预定距离处。 尖端可以是可移除的，并且可以使用具有不同焦距的尖端来适应不同类型的组织和检查。 检测器可以将拉曼光谱转换为信号和数据，以便计算机系统进行分析。 可以对预定位置中的组织的拉曼光谱进行分析，使得系统可以区分健康组织和患病组织。

公开(公告)号：US20120062885A1

公开(公告)日：2012-03-15

申请号：US13255911

申请日：2010-06-21

Applicant: Juqiang Lin ,  Rong Chen ,  Shangyuan Feng ,  Yongzeng Li ,  Guannan Chen ,  Zufang Huang ,  Min Cheng ,  Yun Yu ,  Haishan Zeng

Inventor： Juqiang Lin ,  Rong Chen ,  Shangyuan Feng ,  Yongzeng Li ,  Guannan Chen ,  Zufang Huang ,  Min Cheng ,  Yun Yu ,  Haishan Zeng

IPC: G01J3/44

CPC classification number: G01N1/00 ,  G01N21/00 ,  G01N21/658 ,  G01N27/447

Abstract: This invention provides a body fluid detection method by using surface enhanced Raman spectroscopy. In this method, some biological macromolecules in body fluid samples could be separated with membrane electrophoresis technique firstly. Next, samples are cut off along with the substrates and touched with glacial acetic acid. Transparent colloid formed while incubating. Then add enhancing substrates and continue to incubate and stir. When solid impurities precipitated, stop incubating and stand for layering. In the end, take upper layer resulted to be tested using SERS detection method and build SERS database. This invention successfully eliminated disturbance of other complex components on the SERS detection of protein, DNA and RNA. High quality SERS spectrum obtained is beneficial to the analysis and process of SERS spectrum. Thus body fluid can be differentiated by comparing body fluid SERS spectrum belonging to the healthy people and patients.

Abstract translation: 本发明通过使用表面增强拉曼光谱法提供体液检测方法。 在这种方法中，体液样品中的一些生物大分子首先用膜电泳技术分离。 接下来，将样品与基底一起切割并与冰醋酸接触。 孵化时形成透明胶体。 然后加入增强底物，继续孵育和搅拌。 当固体杂质沉淀时，停止孵育并代替分层。 最后，使用SERS检测方法测试上层，构建SERS数据库。 本发明成功地消除了其他复合成分对蛋白质，DNA和RNA的SERS检测的影响。 获得的高质量SERS光谱有利于SERS光谱的分析和处理。 因此，通过比较属于健康人和患者的体液SERS谱可以区分体液。

公开(公告)号：US20080221457A1

公开(公告)日：2008-09-11

申请号：US10596072

申请日：2004-11-26

Applicant: Haishan Zeng ,  Harvey Lui ,  Zhiwei Huang ,  David I. McLean

Inventor： Haishan Zeng ,  Harvey Lui ,  Zhiwei Huang ,  David I. McLean

IPC: A61B6/00

CPC classification number: A61B5/0071 ,  A61B5/0035 ,  A61B5/0075 ,  A61B5/0086 ,  A61B5/444 ,  A61B5/7264 ,  G01N21/6486 ,  G01N21/65

Abstract: Methods and apparatus for classifying tissue use features of Raman spectra and background fluorescent spectra. The spectra may be acquired in the near-infrared wavelengths. Principal component analysis and linear discriminant analysis of reference spectra may be used to obtain a classification function that accepts features of the Raman and background fluorescence spectra for test tissue and yields an indication as to the likelihood that the test tissue is abnormal. The methods and apparatus may be applied to screening for skin cancers or other diseases.

Abstract translation: 用于分类拉曼光谱和背景荧光光谱的组织使用特征的方法和装置。 可以在近红外波长中获取光谱。 可以使用参考光谱的主成分分析和线性判别分析来获得接受测试组织的拉曼特征和背景荧光光谱的分类函数，并产生关于测试组织异常的可能性的指示。 所述方法和装置可用于筛选皮肤癌或其它疾病。

公开(公告)号：US07383077B2

公开(公告)日：2008-06-03

申请号：US10761703

申请日：2004-01-21

Applicant: Haishan Zeng

Inventor： Haishan Zeng

IPC: A61B5/00

CPC classification number: A61B5/0086 ,  A61B5/0071 ,  A61B5/0075 ,  G01N21/65 ,  G01N2021/656

Abstract: An apparatus for in vivo, endoscopic laser Raman spectroscopy probe and methods for its use are described. The probe comprises a fiber bundle assemble small enough to fit through an endoscope instrument channel, a novel combination of special coatings to the fiber bundle assembly, including a short-pass filter on the illumination fiber and a long-pass filter on the collection fiber bundle, a novel filter adapter comprising collimating lenses, focusing lenses, a band-pass filter, and a notch filter, and a round-to-parabolic linear array fiber bundle. The apparatus further comprises a laser to deliver illumination light and a spectrometer to analyze Raman-scattered light from a sample. The analysis of Raman spectra from in vivo measurements can discover molecular and structural changes associated with neoplastic transformations and lead to early diagnosis and treatment of cancer.

Abstract translation: 描述了一种用于体内内镜激光拉曼光谱探针的装置及其使用方法。 探针包括纤维束，其尺寸足够小以穿过内窥镜仪器通道，将特殊涂层与纤维束组件的新颖组合包括在照明光纤上的短路滤光器和收集纤维束上的长通滤光片 包括准直透镜，聚焦透镜，带通滤光器和陷波滤波器以及圆抛物线性阵列光纤束的新型滤光片适配器。 该装置还包括用于传送照明光的激光和用于分析来自样品的拉曼散射光的光谱仪。 来自体内测量的拉曼光谱分析可以发现与肿瘤转化相关的分子和结构变化，并导致癌症的早期诊断和治疗。