公开(公告)号：US20230290627A1

公开(公告)日：2023-09-14

申请号：US18121550

申请日：2023-03-14

Applicant: Labcyte, Inc. ,  DH Technologies Development Pte. Ltd.

Inventor： Sammy Datwani ,  Don W. Arnold ,  Lucien P. Ghislain ,  Chang Liu ,  Thomas Covey

IPC: H01J49/04 ,  H01J49/16 ,  H01J49/00 ,  B01L3/02 ,  G01N29/22 ,  G01N29/02

CPC classification number: H01J49/0454 ,  H01J49/0404 ,  H01J49/165 ,  H01J49/0031 ,  B01L3/0268 ,  G01N29/222 ,  G01N29/02 ,  H01J49/045 ,  H01J49/0445 ,  B01L2400/0439 ,  B01L2400/0436

Abstract: A system and method are provided for loading a sample into an analytical instrument using acoustic droplet ejection (“ADE”) in combination with a continuous flow sampling probe. An acoustic droplet ejector is used to eject small droplets of a fluid sample containing an analyte into the sampling tip of a continuous flow sampling probe, where the acoustically ejected droplet combines with a continuous, circulating flow stream of solvent within the flow probe. Fluid circulation within the probe transports the sample through a sample transport capillary to an outlet that directs the analyte away from the probe to an analytical instrument, e.g., a device that detects the presence, concentration quantity, and/or identity of the analyte. When the analytical instrument is a mass spectrometer or other type of device requiring the analyte to be in ionized form, the exiting droplets pass through an ionization region, e.g., an electrospray ion source, prior to entering the mass spectrometer or other analytical instrument. The method employs active flow control and enables real-time kinetic measurements.

公开(公告)号：US20210384023A1

公开(公告)日：2021-12-09

申请号：US17410758

申请日：2021-08-24

Applicant: Labcyte, Inc. ,  DH Technologies Development Pte. Ltd.

Inventor： Sammy Datwani ,  Don W. Arnold ,  Lucien P. Ghislain ,  Chang Liu ,  Thomas Covey

IPC: H01J49/04 ,  H01J49/16 ,  H01J49/00 ,  B01L3/02 ,  G01N29/28 ,  G01N29/24 ,  G01N24/00 ,  G01N1/38 ,  G01N29/22 ,  G01N29/02 ,  G01N35/10 ,  B05B17/06

Abstract: A system and method are provided for loading a sample into an analytical instrument using acoustic droplet ejection (“ADE”) in combination with a continuous flow sampling probe. An acoustic droplet ejector is used to eject small droplets of a fluid sample containing an analyte into the sampling tip of a continuous flow sampling probe, where the acoustically ejected droplet combines with a continuous, circulating flow stream of solvent within the flow probe. Fluid circulation within the probe transports the sample through a sample transport capillary to an outlet that directs the analyte away from the probe to an analytical instrument, e.g., a device that detects the presence, concentration quantity, and/or identity of the analyte. When the analytical instrument is a mass spectrometer or other type of device requiring the analyte to be in ionized form, the exiting droplets pass through an ionization region, e.g., an electrospray ion source, prior to entering the mass spectrometer or other analytical instrument. The method employs active flow control and enables real-time kinetic measurements.

公开(公告)号：US20250166984A1

公开(公告)日：2025-05-22

申请号：US18934048

申请日：2024-10-31

Applicant: Labcyte Inc.

Inventor： Chang Liu ,  Jing Ma ,  Heguang Ji ,  Stephen Hinkson ,  Christian Miller ,  Xu Du

IPC: H01J49/04 ,  H01J49/16

Abstract: Systems and methods are disclosed herein that prevent over-production of acoustically ejected droplets into a mass spectrometer, without increase to the time required to conduct pre-ejection sample analysis. This is accomplished by decoupling the repetition rate of energy pulses delivered to the sample during an analysis mode from the repetition rate of energy pulses delivered to the sample during ejection mode. The repetition rate during analysis is higher than the repetition rate used during droplet ejection, which is reduced to a level that can be ingested by the acoustically emitted mass spectrometry system.

公开(公告)号：US11133163B2

公开(公告)日：2021-09-28

申请号：US16985076

申请日：2020-08-04

Applicant: Labcyte, Inc. ,  DH Technologies Development Pte. Ltd.

Inventor： Sammy Datwani ,  Don W. Arnold ,  Lucien P. Ghislain ,  Chang Liu ,  Thomas Covey

IPC: H01J49/04 ,  H01J49/16 ,  H01J49/00 ,  B01L3/02 ,  G01N29/28 ,  G01N29/24 ,  G01N24/00 ,  G01N1/38 ,  G01N29/22 ,  G01N29/02 ,  G01N35/10 ,  B05B17/06

Abstract: A system and method are provided for loading a sample into an analytical instrument using acoustic droplet ejection (“ADE”) in combination with a continuous flow sampling probe. An acoustic droplet ejector is used to eject small droplets of a fluid sample containing an analyte into the sampling tip of a continuous flow sampling probe, where the acoustically ejected droplet combines with a continuous, circulating flow stream of solvent within the flow probe. Fluid circulation within the probe transports the sample through a sample transport capillary to an outlet that directs the analyte away from the probe to an analytical instrument, e.g., a device that detects the presence, concentration quantity, and/or identity of the analyte. When the analytical instrument is a mass spectrometer or other type of device requiring the analyte to be in ionized form, the exiting droplets pass through an ionization region, e.g., an electrospray ion source, prior to entering the mass spectrometer or other analytical instrument. The method employs active flow control and enables real-time kinetic measurements.

公开(公告)号：US20210090867A1

公开(公告)日：2021-03-25

申请号：US16985076

申请日：2020-08-04

Applicant: Labcyte, Inc. ,  DH Technologies Development Pte. Ltd.

Inventor： Sammy Datwani ,  Don W. Arnold ,  Lucien P. Ghislain ,  Chang Liu ,  Thomas Covey

IPC: H01J49/04 ,  H01J49/16 ,  H01J49/00 ,  B01L3/02

Abstract: A system and method are provided for loading a sample into an analytical instrument using acoustic droplet ejection (“ADE”) in combination with a continuous flow sampling probe. An acoustic droplet ejector is used to eject small droplets of a fluid sample containing an analyte into the sampling tip of a continuous flow sampling probe, where the acoustically ejected droplet combines with a continuous, circulating flow stream of solvent within the flow probe. Fluid circulation within the probe transports the sample through a sample transport capillary to an outlet that directs the analyte away from the probe to an analytical instrument, e.g., a device that detects the presence, concentration quantity, and/or identity of the analyte. When the analytical instrument is a mass spectrometer or other type of device requiring the analyte to be in ionized form, the exiting droplets pass through an ionization region, e.g., an electrospray ion source, prior to entering the mass spectrometer or other analytical instrument. The method employs active flow control and enables real-time kinetic measurements.

公开(公告)号：US12154776B2

公开(公告)日：2024-11-26

申请号：US18121550

申请日：2023-03-14

Applicant: LABCYTE INC. ,  DH Technologies Development Pte. Ltd.

Inventor： Sammy Datwani ,  Don W. Arnold ,  Lucien P. Ghislain ,  Chang Liu ,  Thomas Covey

IPC: H01J49/04 ,  B01L3/02 ,  G01N29/02 ,  G01N29/22 ,  H01J49/00 ,  H01J49/16

Abstract: A system and method are provided for loading a sample into an analytical instrument using acoustic droplet ejection (“ADE”) in combination with a continuous flow sampling probe. An acoustic droplet ejector is used to eject small droplets of a fluid sample containing an analyte into the sampling tip of a continuous flow sampling probe, where the acoustically ejected droplet combines with a continuous, circulating flow stream of solvent within the flow probe. Fluid circulation within the probe transports the sample through a sample transport capillary to an outlet that directs the analyte away from the probe to an analytical instrument, e.g., a device that detects the presence, concentration quantity, and/or identity of the analyte. When the analytical instrument is a mass spectrometer or other type of device requiring the analyte to be in ionized form, the exiting droplets pass through an ionization region, e.g., an electrospray ion source, prior to entering the mass spectrometer or other analytical instrument. The method employs active flow control and enables real-time kinetic measurements.

公开(公告)号：US11637006B2

公开(公告)日：2023-04-25

申请号：US17410758

申请日：2021-08-24

Applicant: Labcyte, Inc. ,  DH Technologies Development Pte. Ltd.

Inventor： Sammy Datwani ,  Don W. Arnold ,  Lucien P. Ghislain ,  Chang Liu ,  Thomas Covey

IPC: G01N29/02 ,  H01J49/04 ,  H01J49/16 ,  H01J49/00 ,  B01L3/02 ,  G01N29/22

Abstract: A system and method are provided for loading a sample into an analytical instrument using acoustic droplet ejection (“ADE”) in combination with a continuous flow sampling probe. An acoustic droplet ejector is used to eject small droplets of a fluid sample containing an analyte into the sampling tip of a continuous flow sampling probe, where the acoustically ejected droplet combines with a continuous, circulating flow stream of solvent within the flow probe. Fluid circulation within the probe transports the sample through a sample transport capillary to an outlet that directs the analyte away from the probe to an analytical instrument, e.g., a device that detects the presence, concentration quantity, and/or identity of the analyte. When the analytical instrument is a mass spectrometer or other type of device requiring the analyte to be in ionized form, the exiting droplets pass through an ionization region, e.g., an electrospray ion source, prior to entering the mass spectrometer or other analytical instrument. The method employs active flow control and enables real-time kinetic measurements.

公开(公告)号：US10770277B2

公开(公告)日：2020-09-08

申请号：US16198667

申请日：2018-11-21

Applicant: Labcyte, Inc. ,  DH Technologies Development Pte. Ltd.

Inventor： Sammy Datwani ,  Don W. Arnold ,  Lucien P. Ghislain ,  Chang Liu ,  Thomas Covey

IPC: H01J49/04 ,  H01J49/16 ,  H01J49/00 ,  B01L3/02

Abstract: A system and method are provided for loading a sample into an analytical instrument using acoustic droplet ejection (“ADE”) in combination with a continuous flow sampling probe. An acoustic droplet ejector is used to eject small droplets of a fluid sample containing an analyte into the sampling tip of a continuous flow sampling probe, where the acoustically ejected droplet combines with a continuous, circulating flow stream of solvent within the flow probe. Fluid circulation within the probe transports the sample through a sample transport capillary to an outlet that directs the analyte away from the probe to an analytical instrument, e.g., a device that detects the presence, concentration quantity, and/or identity of the analyte. When the analytical instrument is a mass spectrometer or other type of device requiring the analyte to be in ionized form, the exiting droplets pass through an ionization region, e.g., an electrospray ion source, prior to entering the mass spectrometer or other analytical instrument. The method employs active flow control and enables real-time kinetic measurements.

公开(公告)号：US20190157060A1

公开(公告)日：2019-05-23

申请号：US16198667

申请日：2018-11-21

Applicant: Labcyte, Inc. ,  DH Technologies Development Pte. Ltd.

Inventor： Sammy Datwani ,  Donald W. Arnold ,  Lucien P. Ghislain ,  Chang Liu ,  Thomas Covey

IPC: H01J49/04 ,  H01J49/00

CPC classification number: H01J49/0454 ,  B01L3/0268 ,  B01L2400/0439 ,  H01J49/0031 ,  H01J49/0404 ,  H01J49/165

Abstract: A system and method are provided for loading a sample into an analytical instrument using acoustic droplet ejection (“ADE”) in combination with a continuous flow sampling probe. An acoustic droplet ejector is used to eject small droplets of a fluid sample containing an analyte into the sampling tip of a continuous flow sampling probe, where the acoustically ejected droplet combines with a continuous, circulating flow stream of solvent within the flow probe. Fluid circulation within the probe transports the sample through a sample transport capillary to an outlet that directs the analyte away from the probe to an analytical instrument, e.g., a device that detects the presence, concentration quantity, and/or identity of the analyte. When the analytical instrument is a mass spectrometer or other type of device requiring the analyte to be in ionized form, the exiting droplets pass through an ionization region, e.g., an electrospray ion source, prior to entering the mass spectrometer or other analytical instrument. The method employs active flow control and enables real-time kinetic measurements.