公开(公告)号：US11118171B2

公开(公告)日：2021-09-14

申请号：US17117870

申请日：2020-12-10

Applicant: Nanjing Tech University

Inventor： Hanjie Ying ,  Dong Liu ,  Yong Chen ,  Qingguo Liu ,  Pingkai Ouyang ,  Ming Lei ,  Huanqing Niu ,  Bin Yu ,  Di Zhang ,  Yuhan Yang ,  Shishi Wu

IPC: C12N9/52 ,  C12N1/20 ,  C12N15/52 ,  C12N15/77 ,  C12P13/08 ,  C12R1/15

Abstract: The invention discloses a recombinant Corynebacterium glutamicum for producing lysine by biofilm continuous fermentation and a construction method thereof, wherein the recombinant Corynebacterium glutamicum is constructed by overexpressing a protease gene FtsH in a Corynebacterium glutamicum; and the construction method comprises the following steps of: (1) performing PCR on a genome of the Corynebacterium glutamicum, and amplifying the FtsH gene to obtain an amplified FtsH gene segment; (2) cloning the FtsH gene segment to an overexpression plasmid to obtain a recombinant plasmid; and (3) introducing the recombinant plasmid into the Corynebacterium glutamicum, and screening to obtain the recombinant Corynebacterium glutamicum. The invention has the beneficial effects that the Corynebacterium glutamicum for overexpressing the protease FtsH is constructed in the invention, and a film-forming ability of the Corynebacterium glutamicum is enhanced, such that a yield of continuous immobilized fermentation of the Corynebacterium glutamicum is increased by 38.2% than that of free fermentation of an original bacterium, and a fermentation cycle is shortened by 26.4%.

公开(公告)号：US10400106B2

公开(公告)日：2019-09-03

申请号：US15801210

申请日：2017-11-01

Applicant: NANJING TECH UNIVERSITY

Inventor： Hanjie Ying ,  Lingxia Zhong ,  Chenjie Zhu ,  Chenglun Tang ,  Xiao Zhang ,  Yong Chen ,  Jinglan Wu ,  Tao Gan ,  Jungiang Shan ,  Dong Liu

IPC: C08L97/02 ,  C08L23/06 ,  C08L23/12 ,  C08L25/06 ,  C08L27/06 ,  C08L55/02 ,  C08L69/00 ,  C08L97/00 ,  B29C48/00 ,  B29C48/92 ,  B29C48/16 ,  B29C48/40 ,  B29C48/38 ,  B29K1/00 ,  B29K101/12

Abstract: It discloses a lignin enhanced wood-plastic composite, belonging to the field of polymer based composites. The wood-plastic composite is composed of one or more of plant fiber, lignin, a recycled thermoplastic plastic, mineral powder and a processing aid, and is processed by a screw granulating extrusion process. Compared with the prior art, the mechanical properties, heat stability and aging resistance of a wood-plastic material are improved by adding lignin into the wood-plastic material according to the present invention.

公开(公告)号：US12215123B2

公开(公告)日：2025-02-04

申请号：US18505822

申请日：2023-11-09

Applicant: NANJING TECH UNIVERSITY

Inventor： Hanjie Ying ,  Tao Shen ,  Chenglun Tang ,  Dong Liu ,  Yong Chen ,  Chenjie Zhu ,  Pengpeng Yang ,  Wei Zhuang

IPC: C07H19/20 ,  A61P35/00 ,  C07H19/173

Abstract: A cordycepin-based derived compound with an anti-tumor effect, and a structure of the compound is as shown in formula I; a cordycepin derivative and a pharmaceutical composition thereof provided by the invention have a good anti-tumor proliferation effect; compared with a parent drug, the cordycepin derivative has better affinity to cell membranes, so that a half-life period of in-vivo metabolism of the drug is longer, and in-vivo remaining time of the drug is longer; compared with other nucleoside anti-tumor drugs, the cordycepin derivative and the pharmaceutical composition thereof provided by the invention have wider types and action ranges of tumors, have excellent inhibition effects on a gastric cancer, a pancreatic cancer, a liver cancer, a small cell lung cancer, a colorectal cancer, melanoma, an ovarian cancer and the like, and have lower side effects and better curative effects.

公开(公告)号：US20210155917A1

公开(公告)日：2021-05-27

申请号：US17117870

申请日：2020-12-10

Applicant: Nanjing Tech University

Inventor： Hanjie Ying ,  Dong Liu ,  Yong Chen ,  Qingguo Liu ,  Pingkai Ouyang ,  Ming Lei ,  Huanqing Niu ,  Bin Yu ,  Di Zhang ,  Yuhan Yang ,  Shishi Wu

IPC: C12N9/52 ,  C12N1/20 ,  C12R1/15 ,  C12N15/52 ,  C12N15/77 ,  C12P13/08

Abstract: The invention discloses a recombinant Corynebacterium glutamicum for producing lysine by biofilm continuous fermentation and a construction method thereof, wherein the recombinant Corynebacterium glutamicum is constructed by overexpressing a protease gene FtsH in a Corynebacterium glutamicum; and the construction method comprises the following steps of: (1) performing PCR on a genome of the Corynebacterium glutamicum, and amplifying the FtsH gene to obtain an amplified FtsH gene segment; (2) cloning the FtsH gene segment to an overexpression plasmid to obtain a recombinant plasmid; and (3) introducing the recombinant plasmid into the Corynebacterium glutamicum, and screening to obtain the recombinant Corynebacterium glutamicum. The invention has the beneficial effects that the Corynebacterium glutamicum for overexpressing the protease FtsH is constructed in the invention, and a film-forming ability of the Corynebacterium glutamicum is enhanced, such that a yield of continuous immobilized fermentation of the Corynebacterium glutamicum is increased by 38.2% than that of free fermentation of an original bacterium, and a fermentation cycle is shortened by 26.4%.

公开(公告)号：US20210147887A1

公开(公告)日：2021-05-20

申请号：US17117788

申请日：2020-12-10

Applicant: Nanjing Tech University

Inventor： Hanjie Ying ,  Dong Liu ,  Yong Chen ,  Pingkai Ouyang ,  Qingguo Liu ,  Huanqing Niu ,  Bin Yu ,  Xiwei Peng ,  Ming Lei ,  Xingyuan Cao

IPC: C12P13/08 ,  C12N9/22 ,  C12N9/14 ,  C12N11/12

Abstract: The invention discloses a method for producing lysine by utilizing adsorption and immobilized fermentation of a recombinant Corynebacterium glutamicum, wherein the recombinant Corynebacterium glutamicum is constructed by simultaneously overexpressing an adenosine triphosphate ATPase while knocking out an extracellular nuclease ExeR in a Corynebacterium glutamicum. The recombinant Corynebacterium glutamicum can effectively improve eDNA secretion of the Corynebacterium glutamicum and reduce eDNA degradation of the Corynebacterium glutamicum, so that the Corynebacterium glutamicum can be more easily adsorbed on a surface of a solid carrier for immobilized fermentation, such that a yield of continuous immobilized fermentation of the Corynebacterium glutamicum is increased by 49.67% than that of free fermentation of an original bacterium, and a fermentation cycle is shortened by 29.17%.

公开(公告)号：US12024732B2

公开(公告)日：2024-07-02

申请号：US18253861

申请日：2021-08-18

Applicant: NANJING TECH UNIVERSITY

Inventor： Chenjie Zhu ,  Hanjie Ying ,  Zhuotao Tan ,  Wei Zhuang ,  Yaping Fu ,  Yaoying Han ,  Ming Li ,  Yong Chen ,  Dong Liu ,  Huanqing Niu ,  Pengpeng Yang

IPC: C12P17/16 ,  C12N9/04 ,  C12P17/12 ,  C12P17/14

CPC classification number: C12P17/16 ,  C12N9/0006 ,  C12P17/12 ,  C12P17/14 ,  C12Y101/01001 ,  C12Y101/01255

Abstract: A method for preparing a nitrogen-containing heterocyclic compound and a derivative thereof by an enzymatic-chemical cascade method, comprising: reacting an alcohol, an amine, an alcohol dehydrogenase, a flavin molecule and a coenzyme in a solvent to obtain the nitrogen-containing heterocyclic compound and the derivative thereof; compared with the prior art, the method is a green and economical enzymatic-chemical cascade method, and is used for synthesizing nitrogen-containing heterocyclic compounds and derivatives thereof; compared with a common toxic chemical catalyst, the alcohol dehydrogenase is selected as a catalyst in the method, which has the characteristics of high substrate specificity, no pollution, high catalytic efficiency, no toxic solvents and simple post-treatment.

公开(公告)号：US11098331B2

公开(公告)日：2021-08-24

申请号：US17117788

申请日：2020-12-10

Applicant: Nanjing Tech University

Inventor： Hanjie Ying ,  Dong Liu ,  Yong Chen ,  Pingkai Ouyang ,  Qingguo Liu ,  Huanqing Niu ,  Bin Yu ,  Xiwei Peng ,  Ming Lei ,  Xingyuan Cao

IPC: C12P13/08 ,  C12N11/084 ,  C12N11/096 ,  C12N9/14 ,  C12N9/22 ,  C12N11/12 ,  C12N11/14

Abstract: The invention discloses a method for producing lysine by utilizing adsorption and immobilized fermentation of a recombinant Corynebacterium glutamicum, wherein the recombinant Corynebacterium glutamicum is constructed by simultaneously overexpressing an adenosine triphosphate ATPase while knocking out an extracellular nuclease ExeR in a Corynebacterium glutamicum. The recombinant Corynebacterium glutamicum can effectively improve eDNA secretion of the Corynebacterium glutamicum and reduce eDNA degradation of the Corynebacterium glutamicum, so that the Corynebacterium glutamicum can be more easily adsorbed on a surface of a solid carrier for immobilized fermentation, such that a yield of continuous immobilized fermentation of the Corynebacterium glutamicum is increased by 49.67% than that of free fermentation of an original bacterium, and a fermentation cycle is shortened by 29.17%.