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公开(公告)号：US11940427B2
公开(公告)日：2024-03-26
申请号：US17448063
申请日：2021-09-20
Applicant: Roche Diagnostics Operations, Inc.
Inventor： Benjamin Tiemann , Stefan Quint , Florian Schweinberger
CPC classification number: G01N30/7266 , G01N30/88 , G01N2030/027
Abstract: A liquid chromatography-mass spectrometry (LC-MS) apparatus including an ionization source coupled to a mass spectrometer and a liquid chromatographic (LC) system coupled to the ionization source. The LC system comprises multiple fluidic streams alternately connectable to the ionization source, thereby assigning a detection time window to each fluidic stream from the multiple fluidic streams when connected to the ionization source. The LC-MS apparatus further comprises a controller configured to carry out steps of monitoring an ionization current of the ionization source for the multiple fluidic streams and identifying differences in flow conditions between the multiple fluidic streams based on the monitored ionization current. The controller is further configured to carry out adjusting detection conditions of one or more of the multiple fluidic streams responsive to the identified differences, thereby enabling eluates of interest from each fluidic stream to be detected by the mass spectrometer in the respective detection time window.
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公开(公告)号：US20250069875A1
公开(公告)日：2025-02-27
申请号：US18719252
申请日：2022-12-13
Applicant: Roche Diagnostics Operations, Inc.
Inventor： Stefan Quint , Florian Schweinberger , Benjamin Tiemann , Marius Wagner
IPC: H01J49/00
Abstract: A method for characterization of a mass spectrometry instrument (100) comprising at least one mass analyzing cell (102, 104, 106) is proposed. The method comprising the steps of analyzing a sample (110) comprising at least one substance having a known molecular weight by means of the mass spectrometry instrument (100) so as to provide a mass spectrum (116, 118, 144, 146) of the sample (110), determining an outer envelope and an inner envelope of the mass spectrum (116, 118, 144, 146), calculating a squared difference between the outer envelope and the inner envelope, and determining a deviation of the calculated squared difference from a theoretical mass to charge ratio value of the substance.
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公开(公告)号：US11016098B2
公开(公告)日：2021-05-25
申请号：US16158931
申请日：2018-10-12
Applicant: Roche Diagnostics Operations, Inc.
Inventor： Uwe Kobold , Roland Thiele , Noah Weiss , Benjamin Tiemann
Abstract: A method and an apparatus for determining a concentration of a target analyte in a sample of bodily fluid are disclosed. The method involves providing a sample of bodily fluid including the target analyte, providing an internal standard solution including a mixture of components having a plurality of isotopes of the target analyte, wherein a concentration of each isotope is unknown, adding the internal standard solution to the sample, analyzing the sample including the internal standard solution using a mass spectrometer, creating a sample function curve based on signal intensities, wherein the signal intensities define arbitrary units, transferring an analyte signal into a corresponding arbitrary analyte unit by means of the sample function curve, and transferring the arbitrary analyte unit into the concentration of a target analyte by means of a standardization function representing a curve of concentrations depending on the arbitrary units.
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公开(公告)号：US20230326729A1
公开(公告)日：2023-10-12
申请号：US18333174
申请日：2023-06-12
Applicant: Roche Diagnostics Operations, Inc.
Inventor： Benjamin Tiemann , Daniel Intelmann
CPC classification number: H01J49/0009 , H01J49/063 , H01J49/0031 , H01J49/009 , G01N30/724
Abstract: A method for optimizing at least one parameter setting of at least one mass spectrometry device (110) operating at unit resolution is disclosed. The method comprises the following steps:
a) determining at least one analyte detection window for detecting an analyte of interest with the mass spectrometry device (110), wherein the analyte detection window is defined by a central mass to charge ratio value of the analyte and a predefined width, wherein the central mass to charge ratio value of the analyte is set to a theoretical mass to charge ratio value of the analyte of interest having more than one decimal place and/or a mass to charge ratio value of the analyte of interest determined by a high resolution mass spectrometry measurement having more than one decimal place;
b) determining at least one internal standard detection window for detecting an internal standard substance with the mass spectrometry device (110), wherein the internal standard detection window is defined by a central mass to charge ratio value of the internal standard substance and the pre-defined width, wherein the central mass to charge ratio value of the internal standard substance is set to a mass to charge ratio value of the internal standard substance calculated relative to the analyte of interest and having more than one decimal place and/or to a mass to charge ratio value of the internal standard substance determined by a high resolution mass spectrometry measurement having more than one decimal place.-
公开(公告)号：US20210063361A1
公开(公告)日：2021-03-04
申请号：US16994118
申请日：2020-08-14
Applicant: Roche Diagnostics Operations, Inc.
Inventor： Stefan Quint , Benjamin Tiemann , Florian Schweinberger , Martin Stingl
Abstract: An automated method of monitoring a state of an analyzer is provided including a mass spectrometer (MS) with an electrospray ionization (ESI) source coupled to a liquid chromatography (LC) stream, including monitoring an electrospray ionization current of the ESI source and identifying a condition of multiple conditions of the analyzer based on the monitored ionization current of the ESI source, one of the conditions being a presence of a dead volume in a liquid chromatography stream of the analyzer downstream of an LC column of the LC stream.
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Patent Agency Ranking
公开(公告)号：US12255059B2
公开(公告)日：2025-03-18
申请号：US17805482
申请日：2022-06-06
Applicant: Roche Diagnostics Operations, Inc.
Inventor： Stefan Quint , Florian Schweinberger , Benjamin Tiemann
IPC: H01J49/42
Abstract: A method for multiple transition monitoring of at least one analyte in a sample using a quadrupole mass analyzer is provided and comprises at least one voltage application step, wherein a direct current (DC) voltage and a radio frequency (AC) voltage are applied between two pairs of electrodes of at least one mass filter of the analyzer, wherein the AC voltage has an amplitude VAC and the DC voltage has an applicable voltage VDC, wherein a supplementary AC voltage is superimposed on top of the AC and the DC voltage, wherein an amplitude ΔVDC of the supplementary AC voltage is ≤ V DC , max 2 b + 1 , wherein VDC,max is a maximum voltage output of the DC voltage and b is a bit size of at least one electronics board of the mass filter of the analyzer; and wherein at least one transition of the analyte is determined with at least one detector of the analyzer.
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公开(公告)号：US20210181166A1
公开(公告)日：2021-06-17
申请号：US17113164
申请日：2020-12-07
Applicant: Roche Diagnostics Operations, Inc.
Inventor： Stefan Quint , Marian Roetzer , Florian Schweinberger , Benjamin Tiemann , Marius Wagner , Ralf Zimmermann
Abstract: An analytical system including a mass spectrometer (MS) coupled to liquid chromatography (LC) via an ionization source (IS), and an automated method of maintaining the analytical system in a QC-compliant status are described. The method comprises determining a deviation of the analytical system from a QC-compliant status by determining a deviation of one or more predetermined parameters in an m/z spectrum above one or more predetermined thresholds, triggering an IS and/or MS maintenance procedure upon determining a deviation from the QC-compliant status, determining a return or a failed return to the QC-compliant status during and/or after the IS and/or MS maintenance procedure by determining a return or a failed return respectively of the one or more predetermined parameters in an m/z spectrum below the one or more predetermined thresholds, triggering another IS and/or MS maintenance procedure upon determining a failed return to the QC-compliant status.
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公开(公告)号：US20190388402A1
公开(公告)日：2019-12-26
申请号：US16562629
申请日：2019-09-06
Applicant: Roche Diagnostics Operations, Inc.
Inventor： Ewelina Hegel , Hans-Peter Josel , Eloisa Lopez-Calle , Josef Roedl , Noah Weiss , Benjamin Tiemann
IPC: A61K31/436 , A61K47/18 , A61K9/08
Abstract: The present description relates to a liquid composition having at least one immune-modulatory macrolide compound and an essentially cell-free blood hemolysate. The present description further relates to a method for manufacturing a stabilized immune-modulatory macrolide calibration solution, by a) preparing a solution comprising an essentially cell-free blood hemolysate, b) admixing a predetermined amount of at least one immune-modulatory macrolide into said solution comprising an essentially cell-free blood hemolysate, and, thereby c) manufacturing a stabilized immune-modulatory macrolide calibration solution. Furthermore, the present description relates to kits, uses, devices, methods and to an immune-modulatory macrolide compound calibration solution related thereto.
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9.发明申请公开(公告)号：US20190049460A1
公开(公告)日：2019-02-14
申请号：US16158931
申请日：2018-10-12
Applicant: Roche Diagnostics Operations, Inc.
Inventor： Uwe Kobold , Roland Thiele , Noah Weiss , Benjamin Tiemann
Abstract: A method and an apparatus for determining a concentration of a target analyte in a sample of bodily fluid are disclosed. The method involves providing a sample of bodily fluid including the target analyte, providing an internal standard solution including a mixture of components having a plurality of isotopes of the target analyte, wherein a concentration of each isotope is unknown, adding the internal standard solution to the sample, analyzing the sample including the internal standard solution using a mass spectrometer, creating a sample function curve based on signal intensities, wherein the signal intensities define arbitrary units, transferring an analyte signal into a corresponding arbitrary analyte unit by means of the sample function curve, and transferring the arbitrary analyte unit into the concentration of a target analyte by means of a standardization function representing a curve of concentrations depending on the arbitrary units.
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公开(公告)号：US12123857B2
公开(公告)日：2024-10-22
申请号：US16996225
申请日：2020-08-18
Applicant: Roche Diagnostics Operations, Inc.
Inventor： Marc Kaminski , Stefan Quint , Benjamin Tiemann
CPC classification number: G01N30/88 , G01N30/20 , G06F16/285 , G01N2030/027 , G01N2030/202 , G01N2030/8804
Abstract: A method of monitoring a state of a liquid chromatography (LC) stream of an automated analyzer is provided including automatically monitoring a system pressure of an injection assembly of the liquid chromatography stream to generate a time series of system pressures, classifying the time series in one of two or more predetermined classes indicating different states of the LC stream and triggering a response based on the classification result.
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