Abstract:
In an atomic absorption photometer, depending on a fact whether a background correction is carried out or not, a pattern of a pulse lighting of light sources and a timing of sampling a light receiving signal are changed. Namely, at the time of a background correction measurement, one cycle is divided into three periods, that is, a period of lighting HCL, a period of lighting D2L, and an off period, and at the time of a measurement without a background correction, one cycle is divided into the period of lighting HCL and the off period. Accordingly, at the time of the measurement without the background correction, the period of lighting HCL and the off period become longer, and signal-to-noise ratio of the light receiving signal is improved. Accordingly, an absorbance with high accuracy can be calculated.
Abstract:
At the time of measurement of a sample, before a sample is introduced into a graphite tube, signal voltages which would be detected by a photomultiplier in connection with all programmable combinations of atomizing temperatures of a graphite tube, widths of entrance and exit slits provided in a spectrometer, and wavelengths into which the light is to be decomposed by the diffraction grating are stored in memory beforehand. At the time of measurement of a sample, an amplification factor of the photomultiplier is controlled by a negative high-voltage controller according to measurement requirements, or measurement is performed after an optimum detector signal voltage is set by controlling the amplification factor of the detector signal output from an amplifier.
Abstract:
Output light spectrum P2(null) data from an optical amplifier and input light spectrum P1(null) data of signal light are prepared, the difference between the P2(null) and a value obtained by multiplying the P1(null) by a provisional gain GT is determined (Steps S232), for the obtained spectrum data, a noise removing process such as a moving average process and the like is performed and then, a spline interpolation process is also performed, whereby ASE light spectrum P3(null) data is prepared and an ASE light level P ASE is determined (Steps S233 through S235). In addition, a noise figure-measuring device 10 calculates the number of channels of WDM light and signal light wavelengths of the respective channels based on the P1(null) or P2(null), and performs analysis to calculate a noise figure NF and the like of an appointed wavelength range around the center of each wavelength thus calculated.
Abstract:
An atomic absorption spectrophotometer including a self reverse type hollow cathode lamp and a photomultiplier tube further includes a preliminary tester for measuring a strength of a signal L when a smaller current is supplied to the hollow cathode lamp and a strength of a signal H when a larger current is supplied while a voltage V applied between a cathode and an anode of the photomultiplier is changed. It also includes an optimal voltage detector for detecting a value V0 of the voltage V at which a super ratio U (which is defined as a ratio of a first ratio L0/H0 to a second ratio L1/H1) is closest to unity under a condition that two values V1 and V0 of the voltage V are arbitrarily chosen so that a ratio H1/H0 or a ratio L1/L0 is a predetermined value. The value V0 is used in the following proper measurement of a sample analysis to apply voltage V to the photomultiplier tube of the photometric detector, where a larger current and a smaller current are alternately supplied to the hollow cathode lamp to perform the background correction. This automatically improves the accuracy of the background correction.
Abstract:
A compact and high-performance spectroscope capable of providing a resolution of 0.1 pm or less as in the case of a large-sized spectroscope with an increased focal length and suitable for measuring the spectral distribution of an excimer laser beam. A collimating optical system collimates light under measurement passing through an entrance slit. The collimated light is incident on a diffraction grating and diffracted at angles differing depending on wavelengths. An imaging optical system focuses a beam of light diffracted by the diffraction grating. An exit slit or a light distribution detector is placed in a focal plane of the imaging optical system. A beam diameter-expanding optical system is placed at least between the collimating optical system and the diffraction grating to expand the diameter of the beam of light collimated by the collimating optical system at least in the direction of dispersion of the diffraction grating.
Abstract:
An optical detection system is adapted particularly for detection of biological reactions, especially fluorescent or chemilluminescent reactions. An excitation source, preferably a laser, illuminates a portion of an object to be examined, the portion preferably comprising one microlocation out of an array of microlocations. An intervening optical detection platform serves to direct the excitation radiation, preferably through use of a scanning system, most preferably through use of an x-y mirror-based scanning system to the portion of the object to be illuminated. A detector, preferably a photomultiplier tube, receives the emitted radiation from the objects to be examined, the detector being characterized in that the diameter of the region examined by the detector is the same as or smaller than the diameter of the illuminated region, and comprises less than the entire surface of the object to be examined, and most preferably images a whole or a part of a single microlocation. Preferably, the excitation source is coupled to the optical detection platform via an optical fiber. In operation, a confocal microscopy system is formed in which the excitation radiation is substantially in focus at the surface of the object to be examined, the excitation radiation having a lateral extent less than the entire diameter of the object to be examined and the detection system having a lateral field of view of a diameter substantially the same as or less than the diameter of the excitation region. In one aspect of this invention, the optical detection platform includes an excitation detector which measures reflected excitation radiation from the object to be examined. This information is then compared to prestored information regarding the location of the microlocations and interstitial regions on the object to be examined, whereby alignment information is obtained. The excitation radiation may then be precisely directed to a given microlocation or portion thereof so as to perform the examining through the confocal system. Significant increases in signal-to-noise ratio are achieve.