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公开(公告)号：KR101415836B1
公开(公告)日：2014-07-09
申请号：KR1020120076917
申请日：2012-07-13
Applicant: 서울대학교산학협력단 , 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N7/01 , C12N15/63 , C12N15/33 , A61K39/145
Abstract: 조류 인플루엔자 바이러스의 HA 단백질 유래의 HA2 절편의 154번째 아미노산인 아스파라긴(N)에 N-글리칸 생성이 일어나지 않도록 154번째 아미노산인 아스파라긴 또는 156번째 아미노산인 세린(S)이나 트레오닌(T)을 다른 아미노산으로 치환하여 HA2 공통 에피톱에 대한 면역원성을 증가시키는 방법과 이러한 방법을 통해 면역원성이 증가된 재조합 바이러스가 제공된다. 본 발명의 HA2 공통 에피톱 면역원성 향상기술과 HA2 공통 에피톱 고면역원성 바이러스는 인플루엔자 예방을 위한 백신주 개발과 백신주로써 유용하게 이용될 수 있다.
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公开(公告)号：KR101423695B1
公开(公告)日：2014-08-04
申请号：KR1020120076916
申请日：2012-07-13
Applicant: 서울대학교산학협력단 , 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본 발명은 저병원성 조류 인플루엔자 바이러스의 PB2 단백질 코딩 폴리뉴클레오타이드를 유효성분으로 포함하는 H1N1 계통 조류 인플루엔자 바이러스 발육계란 고증식성, 마우스 무병원성 조성물, 상기 폴리뉴클레오타이드를 포함하는 재조합 발현 벡터, 상기 발현 벡터로 형질전환 된 약독화 재조합 바이러스, 및 상기 재조합 바이러스를 포함하는 백신 조성물에 관한 것이다. 본 발명의 재조합 발현 벡터는 인플루엔자 예방을 위한 백신주의 계태아 생산성을 높여주어 사독백신 효능 및 생산성 향상에 유용하게 이용될 수 있고, 마우스에 대한 병원성이 없고, 면역원성이 있어 생독백신에 활용될 수 있다는 장점을 갖는다.
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公开(公告)号：KR1020140010583A
公开(公告)日：2014-01-27
申请号：KR1020120076917
申请日：2012-07-13
Applicant: 서울대학교산학협력단 , 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N7/01 , C12N15/63 , C12N15/33 , A61K39/145
Abstract: Provided are: a method for improving immunogenicity of a HA2 common epitope of influenza virus by replacing asparagine, which is the 154th amino acid, and serine (S) or threonine (T), which is the 156the amino acid, with other amino acid such that N-glycan generation does not occur at asparagine (N), which is the 154th amino acid, of a HA2 fragment derived from a HA amino acid of avian influenza virus; and a recombinant virus with improved immunogenicity through the method. The technic for improving immunogenicity of a HA2 common epitope and the HA2 common epitope high immunogenicity virus of the present invention can be effectively used in developing a vaccine strain for preventing influenza and effectively used as a vaccine strain.
Abstract translation: 提供：通过将作为第154位氨基酸的天冬酰胺和作为氨基酸的156位的丝氨酸（S）或苏氨酸（T）与其它氨基酸这样的氨基酸取代来改善流感病毒的HA2常见表位的免疫原性的方法 来自禽流感病毒的HA氨基酸的HA2片段的第155位氨基酸的天冬酰胺（N）不发生N-聚糖生成; 和通过该方法具有改善的免疫原性的重组病毒。 用于提高本发明的HA2常见表位和HA2常见表位高免疫原性病毒的免疫原性的技术可以有效地用于开发用于预防流行性感冒并有效用作疫苗株的疫苗株。
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公开(公告)号：KR1020140010582A
公开(公告)日：2014-01-27
申请号：KR1020120076916
申请日：2012-07-13
Applicant: 서울대학교산학협력단 , 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: The present invention relates to: a composition for high productiveness of an embryonated egg of H1N1 family avian influenza and non-pathogenicity of a mouse including, as a active ingredient, a polynucleotide encoding a PB2 protein of a low pathogenic avian influenza virus; a recombinant expression vector including the polynucleotide; an attenuated recombinant virus transformed into the expression vector; and a vaccine composition including the recombinant expression vector. The recombinant expression vector of the present invention enhances chicken embryo productivity of a vaccine strain for preventing influenza, and thereby, having advantages of being capable of being effectively used in enhancing efficacy and productivity of killed vaccines, and additionally, has no pathogenicity for mice and has immunogenicity, and thereby having advantages of being capable of being effectively used as live vaccines. [Reference numerals] (AA) Inserting into the inside of pHW2000-BsmBI
Abstract translation: 本发明涉及一种用于H1N1家禽禽流感的胚胎卵和非致病性的高生产力的组合物，其包含作为活性成分的编码低致病性禽流感病毒的PB2蛋白的多核苷酸; 包含该多核苷酸的重组表达载体; 减毒的重组病毒转化成表达载体; 以及包含重组表达载体的疫苗组合物。 本发明的重组表达载体增强用于预防流感的疫苗株的鸡胚生产力，从而具有能够有效地用于提高杀死疫苗的功效和生产力的优点，此外，对小鼠没有致病性， 具有免疫原性，因此具有能够有效地用作活疫苗的优点。 （标号）（AA）插入pHW2000-BsmBI的内部
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公开(公告)号：KR101557191B1
公开(公告)日：2015-10-02
申请号：KR1020130075529
申请日：2013-06-28
Applicant: 조지아 스테이트 유니버시티 리서치 파운데이션, 인코포레이티드 , 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C07K14/11 , C12N7/01 , A61K39/145 , A61P31/16
Abstract: 본발명은넓은범위의방어능력을갖는인플루엔자단독백신또는이를이용한인플루엔자보강백신에관한것이다. 본발명의인플루엔자단독백신또는인플루엔자보강백신은다양한혈청형의인플루엔자바이러스감염에대하여 M2e단백질에대한면역력을증가시킴으로써전국적유행병과같이예측하지못한바이러스의확산에효과적으로대처할수 있는광범위한방어능력을가진다.
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Patent Agency Ranking6.发明授权신속 면역크로마토그라피법에 의한 Ｈ9형 조류인플루엔자 바이러스 진단 키트 및 이를 이용하여 Ｈ9형 조류인플루엔자를 진단하는 방법 有权使用快速免疫层析技术的H9型禽流感病毒诊断试剂盒及使用相同方法诊断H9型禽流感
公开(公告)号：KR101211593B1
公开(公告)日：2013-01-07
申请号：KR1020100087163
申请日：2010-09-06
Applicant: 대한민국(농림축산식품부 농림축산검역본부장) , 주식회사 바이오노트
IPC: G01N33/569 , G01N33/538 , G01N33/553 , C07K16/08
Abstract: 본발명은헤마글루티닌항원이 H9형조류인플루엔자에특이적인항체를이용한신속면역크로마토그라피법에의해닭 총배설강(분변) 스왑시료에서 H9형조류인플루엔자감염여부를신속하고정확하게진단할수 있는진단키트및 이를이용하여 H9형조류인플루엔자를진단하는방법에관한것이다.
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7.发明公开신속 면역크로마토그라피법에 의한 Ｈ5형 고병원성 및 일반조류인플루엔자 바이러스 항원 진단 스트립 및 그 제조방법 有权用于H5型高致病性流感病毒性流行性感冒病毒和使用快速免疫印迹的一般流行性流感病毒抗原的诊断条带及其制备方法
公开(公告)号：KR1020070072945A
公开(公告)日：2007-07-10
申请号：KR1020060000325
申请日：2006-01-03
Applicant: 대한민국(농림축산식품부 농림축산검역본부장) , 주식회사 바이오노트
IPC: G01N33/53
Abstract: A diagnostic strip for a H5 type highly pathogenic avian influenza and general avian influenza is provided to conveniently and rapidly diagnose whether a bird is infected by the H5 type highly pathogenic avian influenza and general avian influenza or not from stool in situ without special testing equipment. The diagnostic strip is characterized in that a monoclonal antibody common to general avian influenza virus in which hemaglutinin antigens are H1 to H15 and a monoclonal antibody specific to a highly pathogenic avian influenza in which a hemaglutinin antigen is H5 are used for diagnosing the highly pathogenic and the general avian influenza in which the hemaglutinin antigen is H5 from stool or tissue of chicken. The method for preparing the diagnostic strip comprises the steps of: (a) respectively absorbing the monoclonal antibody specific to the highly pathogenic avian influenza virus in which the hemaglutinin antigen is H5 and the monoclonal antibody common to general avian influenza virus into a membrane; (b) respectively attaching the antibody absorbed membrane obtained from the step(a) to gold particles so as to prepare an antibody-gold conjugate and then mixing it; and (c) precipitating the conjugate obtained from the step(b) in a basic material and then drying it.
Abstract translation: 提供一种H5型高致病性禽流感和一般禽流感的诊断条，以方便快捷地诊断鸟类是否由H5型高致病性禽流感和一般禽流感感染，或者不是在没有特殊检测设备的情况下从粪便中感染。 诊断条的特征在于，其中血凝素抗原为H1至H15的一般性禽流感病毒共有的单克隆抗体和其中血凝素抗原为H5的高致病性禽流感特异性的单克隆抗体用于诊断高致病性和 其中血凝素抗原为粪便或鸡组织中H5的一般性禽流感。 制备诊断条的方法包括以下步骤：（a）分别将其中血凝素抗原为H5的高致病性禽流感病毒和一般禽流感病毒共有的单克隆抗体特异性的单克隆抗体吸收到膜中; （b）将由步骤（a）获得的抗体吸收膜分别附着于金颗粒上，制备抗体 - 金缀合物，然后混合; 和（c）将由步骤（b）获得的缀合物沉淀在碱性材料中，然后干燥。
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公开(公告)号：KR1020070005867A
公开(公告)日：2007-01-10
申请号：KR1020050060920
申请日：2005-07-06
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: A virus strain for preparing a vaccine is provided to have excellent defending effect on low pathogenic avian influenza H9N2 virus strain. And a vaccine is provided to be able to effectively prevent diseases caused by the low pathogenic avian influenza H9N2 recording the high mortality of bird. The low pathogenic avian influenza H9N2 virus strain for preparing vaccine is characterized in that a motif of hemangioblast protein cleavage site amino acid sequence has TSGR and it shows the defending capacity against domestic low pathogenic avian influenza virus. The method for preparing a vaccine for the low pathogenic avian influenza H9N2 virus strain comprises the steps of: (a) inactivating the low pathogenic avian influenza H9N2 virus strain; and (b) mixing the inactivated virus with an adequate excipient.
Abstract translation: 提供用于制备疫苗的病毒株，以对低致病性禽流感H9N2病毒株具有优异的防御作用。 并提供疫苗能够有效预防由低致病性禽流感H9N2引起的疾病，记录鸟类的高死亡率。 用于制备疫苗的低致病性禽流感H9N2病毒株的特征在于成血管细胞蛋白切割位点氨基酸序列的基序具有TSGR，并且显示出针对国内低致病性禽流感病毒的防御能力。 用于制备低致病性禽流感H9N2病毒株的疫苗的方法包括以下步骤：（a）灭活低致病性禽流感H9N2病毒株; 和（b）将灭活的病毒与足够的赋形剂混合。
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公开(公告)号：KR1020060132154A
公开(公告)日：2006-12-21
申请号：KR1020050052324
申请日：2005-06-17
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: A recombinant protein of avian influenza virus and a diagnostic composition of avian influenza containing the same protein are provided to mass produce the antigen for diagnosis of avian influenza cheaply, so that the disease caused by avian influenza is prevented. The method for producing the recombinant nucleus protein of avian influenza virus comprises the steps of: (1) amplifying the gene encoding nucleocapsid having the nucleotide sequence of SEQ ID NO:1 as a common protein of MS96 strain with PCR, and cloning the PCR amplified gene into the TA cloning vector; (2) re-isolating the NP gene inserted into the TA cloning vector with a restriction enzyme EcoR I, and inserting the isolated NP gene into the baculovirus transition vector pBacPAK8 to prepare the baculovirus transition vector pBacAINP; (3) co-transfecting the Sf21 cell with the baculovirus and the transition vector pBacAINP to a recombinant baculovirus(rAINP) expressing the avian influenza virus NP protein; and (4) verifying the expression of avian influenza virus NP(nucleus protein) in the Sf21 cell by using a monoclonal antibody specific to the avian influenza virus NP.
Abstract translation: 提供禽流感病毒的重组蛋白和含有相同蛋白质的禽流感诊断组合物，以便大量生产用于禽流感诊断的抗原，从而防止禽流感引起的疾病。 制备禽流感病毒重组核蛋白的方法包括以下步骤：（1）用PCR扩增编码具有SEQ ID NO：1的核苷酸序列的核衣壳的基因作为MS96菌株的常见蛋白质，并将PCR扩增 基因进入TA克隆载体; （2）用限制酶EcoR I重新分离插入TA克隆载体的NP基因，将分离的NP基因插入杆状病毒转移载体pBacPAK8中以制备杆状病毒转移载体pBacAINP; （3）将杆状病毒和过渡载体pBacAINP的Sf21细胞共转染至表达禽流感病毒NP蛋白的重组杆状病毒（rAINP）; 和（4）通过使用特异于禽流感病毒NP的单克隆抗体来验证Sf21细胞中禽流感病毒NP（核蛋白）的表达。
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公开(公告)号：KR1020100136191A
公开(公告)日：2010-12-28
申请号：KR1020090054422
申请日：2009-06-18
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: PURPOSE: An antigen for hemoglutination for swine Japanese encephalitis virus is provided to reduce pollution caused by acetone extraction. CONSTITUTION: A method for preparing antigen for hemoglutination of high concentration for swine Japanese encephalitis virus comprises: a step of inoculating swine Japanese encephalitis virus to a tissue culture cells and planting; a step of freezing culture liquid; a step of thawing the culture liquid and repeating freezing and thawing once; a step of collecting cultured swine Japanese encephalitis virus; a step of inactivating swine Japanese encephalitis virus; a step of concentrating swine Japanese encephalitis virus; and a step of precipitating swine Japanese encephalitis virus by centrifugation.
Abstract translation: 目的：提供用于猪日本脑炎病毒血凝反应的抗原，以减少丙酮提取引起的污染。 构成：一种制备猪日本脑炎病毒高浓度血凝反应抗原的方法，包括：将日本脑炎病毒接种在组织培养细胞中并种植; 冷冻培养液的一个步骤; 解冻培养液并重复冻融一次; 收集日本猪日本脑炎病毒的一步; 灭活日本脑炎病毒的一步; 日本脑炎病毒浓缩一步; 并通过离心沉淀猪日本脑炎病毒的一个步骤。
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